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超声造影对鼻咽癌放化疗疗效的预测研究 被引量:1
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作者 高泳 卢露 廖新红 《右江民族医学院学报》 2020年第3期286-290,共5页
目的探讨鼻咽部超声造影预测鼻咽癌原发灶放化疗疗效的价值。方法收集广西医科大学第一附属医院放疗科拟接受放化疗治疗的鼻咽癌患者共20例,所有患者均经鼻内窥镜活检病理证实。于治疗前分别行鼻咽部超声造影和常规磁共振成像(MRI)扫描... 目的探讨鼻咽部超声造影预测鼻咽癌原发灶放化疗疗效的价值。方法收集广西医科大学第一附属医院放疗科拟接受放化疗治疗的鼻咽癌患者共20例,所有患者均经鼻内窥镜活检病理证实。于治疗前分别行鼻咽部超声造影和常规磁共振成像(MRI)扫描,分别勾画肿瘤超声造影平均强化区、高增强区及低增强区,获取超声造影时间-强度曲线(TIC)及相关定量参数;于治疗结束13周时再次行常规MRI扫描。根据实体瘤疗效评价标准将患者分为CR组和非CR组,采用成组设计t检验分析CR组与非CR组间肿瘤不同强化区域超声造影定量参数的差异性;采用Spearman等级相关分析评价肿瘤不同强化区域超声造影定量参数与鼻咽癌治疗疗效的相关性。结果超声造影定量参数中,CR组与非CR组间肿瘤平均强化区、高增强区、低增强区的峰值强度(PI)、平均渡越时间(MTT)、衰减速率(Slope)值差异均具有统计学意义(P<0.05),其中CR组PI、Slope值均高于非CR组,而MTT值低于非CR组。CR组与非CR组间肿瘤高增强区的达峰时间(TTP)差异具有统计学意义(P<0.05),且CR组低于非CR组。肿瘤不同强化区域的PI值、Slope均与治疗效果呈正相关,而TTP均与治疗效果呈负相关;平均强化区、低增强区的MTT与治疗效果呈负相关。鼻咽癌病灶超声造影TIC曲线主要表现为两型:Ⅰ型(速升-速降-缓降型)和Ⅱ型(速升-速降-平台型)。CR组中,肿瘤平均强化区和高增强区TIC曲线均以Ⅰ型为主;非CR组中,低增强区TIC曲线以Ⅱ型为主。结论鼻咽部超声造影定量参数可以较好预测鼻咽癌治疗的敏感性,可为临床制定个体化治疗方案提供有用的影像学资料。 展开更多
关键词 鼻咽肿瘤癌 超声造影 MRI 放化疗 定量参数
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同步放化疗治疗晚期鼻咽癌远期疗效评价 被引量:12
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作者 熊强 胡蓉环 +3 位作者 刘建辉 罗旭俊 刘光金 丁剑午 《实用临床医学(江西)》 CAS 2005年第10期37-38,共2页
目的:研究同步放化疗对晚期鼻咽癌远期疗效的影响.方法:128例Ⅲ、Ⅳa期鼻咽癌随机分成两组(单放组和放化同步组).单放组64例,采用6MEV,直线加速器,以常规分割方式放疗,鼻咽部剂量68~70 GY,颈部根治量60~68 GY,预防量46~50 GY;同步放... 目的:研究同步放化疗对晚期鼻咽癌远期疗效的影响.方法:128例Ⅲ、Ⅳa期鼻咽癌随机分成两组(单放组和放化同步组).单放组64例,采用6MEV,直线加速器,以常规分割方式放疗,鼻咽部剂量68~70 GY,颈部根治量60~68 GY,预防量46~50 GY;同步放化组64例,除了按上述放疗外,并同步行DDP30mg+5-Fu0.75g+CF0.2 g连续静脉点滴5 d化疗,并在放疗结束前一周,再行同样化疗一周期.结果:单放组和同步放化组肿瘤消退率分别为81.25%、95.75%(P>0.05),颈部转移灶完全消退率分别为84.375%、90.625%(P>0.05).五年无瘤生存率分别为25%,39.06%(P<0.05).五年生存率分别为37.5%、56.25%(P<0.05).远处转移率分别为37.5%、12.5%(P<0.05).出现远处转移平均时间单放组16个月,放化同步组32个月(P<0.02).结论:同步放化组在三年、五年总生存率(OS)和无瘤生存率(DPS)方面及出现远处转移率和转移平均时间方面均要优于单放组,且有显著性差异. 展开更多
关键词 肿瘤() 同步放化疗 单纯放射治疗
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血清EB病毒特异性DNA酶抗体与鼻咽癌分期的关系 被引量:2
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作者 李泽卿 周玫 +3 位作者 田勇泉 肖健云 赵素萍 陶正德 《医学研究生学报》 CAS 2000年第4期221-223,共3页
目的 :探讨血清非洲淋巴细胞瘤病毒特异性 DNA酶 ( Epstein-Barr virus-specific deoxynuclease,EBV-DNase)抗体与鼻咽癌 ( nasopharyngeal carcinoma,NPC)分期的关系。方法 :用 Western Blot法检测 2 64例 NPC血清 ,按“92分期法”对... 目的 :探讨血清非洲淋巴细胞瘤病毒特异性 DNA酶 ( Epstein-Barr virus-specific deoxynuclease,EBV-DNase)抗体与鼻咽癌 ( nasopharyngeal carcinoma,NPC)分期的关系。方法 :用 Western Blot法检测 2 64例 NPC血清 ,按“92分期法”对所有血清进行分期 ,分别计算各 T、N、M期及临床病期的 EBV-DNase抗体阳性率并进行统计学处理。 结果 :NPC各 T、N、M组及临床分期组血清 EBV-DNase抗体阳性率均无显著差异 ( P>0 .0 5 )。 结论 :早期 ( 期 ) NPC血清 EBV-DNase抗体阳性率与 ~ 期血清无明显差异 ,EBV-DNase抗体有可能成为 展开更多
关键词 肿瘤 EB病毒特异性DNA酶抗体 WESTERN BLOT法 诊断
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Screening for nasopharyngeal carcinoma in high-incidence regions——Next steps
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作者 Allan Hildesheim 《广西医科大学学报》 CAS 2024年第9期1247-1251,共5页
Epstein-Barr virus(EBV)infection is a well-established risk factor in the development of nonkeratinizing and undifferentiated forms of nasopharyngeal carcinoma(NPC)common in parts of China and Southeast Asia.Early det... Epstein-Barr virus(EBV)infection is a well-established risk factor in the development of nonkeratinizing and undifferentiated forms of nasopharyngeal carcinoma(NPC)common in parts of China and Southeast Asia.Early detection of NPC can significantly improve survival rates,as the 5-year survival rate for patients diagnosed at an early stage can exceed 90%after treatment.Studies have demonstrated that screening for NPC using EBV markers is an effective tool for identifying individuals with the disease.Future efforts should focus on implementing screening programs in high-incidence populations,assessing and refining screening algorithms,and exploring new,potentially more cost-effective screening methods.It is crucial to ensure that any new approaches are validated as superior or non-inferior to existing protocol before being adopted on a wider scale.The success of these screening tools in reducing NPC-related morbidity and mortality will depend on their effective implementation and ensuring access for the populations most in need of preventive interventions.This opinion piece briefly summarizes the current evidence supporting EBV-based screening for NPC detection and discusses future steps,including:1)the implementation of effective NPC screening programs,2)the evaluation of improvements in screening methodologies,and 3)the consideration of novel approaches to screening. 展开更多
关键词 nasopharyngeal carcinoma Epstein-Barr virus SCREENING prevention cancer
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Significance of the Expression of CyclinD1 and Ki67 Antigen in Nasopharyngeal Carcinoma 被引量:1
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作者 胡国清 石小燕 《The Chinese-German Journal of Clinical Oncology》 CAS 2004年第1期24-28,65,共6页
Objective: To detect the expression of cyclinD1 and Ki67 in nasopharyngeal carcinoma (NPC) and their correlation with the biological behaviors and prognosis. Methods: 56 cases of biopsy specimens ... Objective: To detect the expression of cyclinD1 and Ki67 in nasopharyngeal carcinoma (NPC) and their correlation with the biological behaviors and prognosis. Methods: 56 cases of biopsy specimens of NPC which had been embedded with para?n in 1996 in our hospital were collected and immunostained with cyclinD1 and Ki67 monoclonal antibodies by means of the streptavidin peroxides method. The patients were followed up periodically, and then their biological behaviors and prognosis were statistically analyzed. Results: The percentage of cyclinD1 and Ki67 positive cells in the NPC specimens ranged from 0–54% and 0–31% respectively. The staining was nuclear. Of the 56 cases, 30 cases (56.6%) highly expressed cyclinD1 or Ki67 HPI and 26 cases (46.4%) lowly expressed cyclinD1, while only 16 cases (28.6%) showed Ki67 HPI (high proliferated index) and 40 cases (71.4%) showed Ki67 LPI (low proliferated index). Patients who lowly expressed cyclinD1 or highly expressed Ki67 had a higher radiosensitivity and a better prognosis. Conclusion: CyclinD1 and Ki67 immunohistochemical staining is considered to be useful, not only as an independent factor of radiosensitivity and prognosis respectively, but also as a means of determining the optimum treatment for each individual patient with NPC. 展开更多
关键词 CYCLIND1 KI67 RADIOSENSITIVITY prognosis nasopharyngeal carcinoma
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EGFR靶向性MRI探针的制备及其与NSCC细胞靶向结合的研究
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作者 刘成 李烁 张全明 《中国现代医学杂志》 CAS 2020年第3期22-26,共5页
目的探讨表皮生长因子受体(EGFR)靶向性磁共振成像(MRI)探针的制备及其与鼻咽部鳞状细胞癌(NSCC)细胞靶向结合的研究。方法利用NSCC表面EGFR作为靶受体,构建一种具有EGFR靶向性MRI探针,检测探针的性质,将探针与NSCC和正常鼻咽细胞孵育,... 目的探讨表皮生长因子受体(EGFR)靶向性磁共振成像(MRI)探针的制备及其与鼻咽部鳞状细胞癌(NSCC)细胞靶向结合的研究。方法利用NSCC表面EGFR作为靶受体,构建一种具有EGFR靶向性MRI探针,检测探针的性质,将探针与NSCC和正常鼻咽细胞孵育,进行普鲁士蓝染色、间接免疫荧光及MRI,观察铁染色、荧光染色及T2WI信号强度变化,并计算信号强度变化率。结果EGFR靶向性MRI探针的粒径为(77.0±1.5)nm,超顺磁性氧化铁(SPIO)负载率为(306.0±4.9)μg/ml,阿霉素(DOX)负载率为(103.3±3.1)μg/ml,弛豫率为(110.4±2.9),提示EGFR靶向性MRI探针制备成功。EGFR靶向性MRI探针与NSCC细胞共孵育后,细胞内可见不同量的蓝色铁Fe颗粒,随着SPIO浓度的升高而增加;EGFR靶向性MRI探针与正常鼻咽细胞共孵育,细胞内未见明显铁颗粒存在。西妥昔单抗(阳性对照)对NSCC细胞膜有强烈的绿色荧光表达,阴性对照呈现很微弱的绿色荧光表达,EGFR靶向性MRI探针与DOX荧光强度相近。随浓度5、10、20、40和80 mg/ml梯度增高,EGFR靶向性MRI探针T1信号基本无变化,EGFR靶向性MRI探针T2信号强度逐渐减弱,EGFR靶向性MRI探针在20、40和80 mg/ml 3个时间点比较,T2信号强度明显下降,其中80 mg/ml时T2信号强度下降最显著。EGFR靶向性MRI探针与NSCC细胞共孵育后MRI信号在T2WI上有不同程度的降低(P<0.05),SPIO浓度越高MRI信号强度降低越明显(P<0.05)。结论EGFR靶向性MRI探针的粒径小,可降低MRI的T2信号强度,具有高度的靶向性。 展开更多
关键词 部鳞状细胞/肿瘤 表皮生长因子受体 多功能磁共振成像探针
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Regulation of Survivin and CDK4 by Epstein-Barr virus encoded latent mem-brane protein 1 in nasopharyngeal carcinoma cell lines 被引量:8
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作者 Mi Dan AI Li Li LI +3 位作者 Xiao Rong ZHAO Yong WU Jian Ping GONG Ya CAO 《Cell Research》 SCIE CAS CSCD 2005年第10期777-784,共8页
Latent membrane protein 1 (LMP1), an important protein encoded by Epstein Barr virus (EBV), has been implied to link with the pathogenesis of nasopharyngeal carcinoma (NPC). Its dual effects of increasing cell p... Latent membrane protein 1 (LMP1), an important protein encoded by Epstein Barr virus (EBV), has been implied to link with the pathogenesis of nasopharyngeal carcinoma (NPC). Its dual effects of increasing cell proliferation and inhibiting cell apoptosis have been confirmed. In this study, we showed that the expression of Survivin and CDK4 protein in CNE-LMP1, a LMP1 positive NPC epithelial cell line, is higher than in LMP1 negative NPC epithelial cell line- CNE1, and the expression is LMP1 dosage-dependent. Although it was reported that Survivin specifically expressed in cell cycle G2/M phase, our studies suggested that LMP1 could promote the expression of Survivin in G0/G1, S and G2/ M phase. It also showed that Survivin and CDK4 could be accumulated more in the nuclei triggered by LMP1. More interestingly, Survivin and CDK4 could form a protein complex in the nuclei of CNE-LMP1 rather than in that of CNE1, which demonstrated that the interaction between these two proteins could be promoted by LMPI. These results strongly suggested that the role of LMP1 in the regulation of Survivin and CDK4 may also shed some light on the mechanism research of LMP1 in NPC. 展开更多
关键词 EBV latent membrane protein 1 cell cycle G1/S check point SURVIVIN CDK4. EBV latent membrane protein 1 cell cycle G1/S check point SURVIVIN CDK4.
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Involvement of regulatory volume decrease in the migration of nasopharyn-geal carcinoma cells 被引量:9
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作者 JianWenMAO LiWeiWANG +7 位作者 TimJACOB XueRongSUN HuiLI LinYanZHU PanLI PingZHONG SiHuaiNIE LiXinCHEN 《Cell Research》 SCIE CAS CSCD 2005年第5期371-378,共8页
The transwell chamber migration assay and CCD digital camera imaging techniques were used to investigate the relationship between regulatory volume decrease (RVD) and cell migration in nasopharyngeal carcinoma cells (... The transwell chamber migration assay and CCD digital camera imaging techniques were used to investigate the relationship between regulatory volume decrease (RVD) and cell migration in nasopharyngeal carcinoma cells (CNE-2Z cells). Both migrated and non-migrated CNE-2Z cells, when swollen by 47% hypotonic solution, exhibited RVD which was inhibited by extracellular application of chloride channel blockers adenosine 5’-triphosphate (ATP), 5-nitro-2-(3- phenylpropylamino) benzoic acid (NPPB) and tamoxifen. However, RVD rate in migrated CNE-2Z cells was bigger than that of non-migrated cells and the sensitivity of migrated cells to NPPB and tamoxifen was higher than that of non- migrated cells. ATP, NPPB and tamoxifen also inhibited migration of CNE-2Z cells. The inhibition of migration was positively correlated to the blockage of RVD, with a correlation coefficient (r) = 0.99, suggesting a functional relation- ship between RVD and cell migration. We conclude that RVD is involved in cell migration and RVD may play an important role in migratory process in CNE-2Z cells. 展开更多
关键词 tumor cells chloride channel blockers cell volume cell migration transwell chamber.
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Mechanisms of cell immortalization mediated by EB viral activation of telomerase in nasopharyngeal carcinoma 被引量:7
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作者 LucyCassar AlexPinto 《Cell Research》 SCIE CAS CSCD 2006年第10期809-817,共9页
Nasopharyngeal carcinoma (NPC) is a common cancer in Southern China and Southeast Asia. The disease is a poorly differentiated carcinoma without effective cure, and the mechanism underlying its development remains l... Nasopharyngeal carcinoma (NPC) is a common cancer in Southern China and Southeast Asia. The disease is a poorly differentiated carcinoma without effective cure, and the mechanism underlying its development remains largely unknown. Of several factors identified in NPC aetiology in recent years, Epstein-Barr virus (EBV) infection has emerged to be most important. In almost all NPC cells, EBV uses several intracellular mechanisms to cause oncogenic evolution of the infected cells. One such mechanism by which EBV infection induces cellular immortalization is believed to be through the activation of telomerase, an enzyme that is normally repressed but becomes activated during cancer development. Studies show that greater than 85% of primary NPC display high telomerase activity by mechanisms involving EBV infection, consistent with the notion that EBV is commonly involved in inducing cell immortalization. More recently, different EBV proteins have been shown to activate or inhibit the human telomerase reverse transcriptase gene, by modulating intracellular signalling pathways. These findings suggest a new model with a number of challenges towards our understanding, molecular targeting and therapeutic intervention in NPC. 展开更多
关键词 EBV tumour antigen TELOMERASE TELOMERES cell immortalization carcinoma NASOPHARYNX
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NASOPHARYNGEAL CARCINOMA RADIOSENSITIVITY PREDICTION BY CYTOKINESIS-BLOCK MICRONUCLEUS ASSAY
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作者 杨星 史剑慧 程文英 《Nuclear Science and Techniques》 SCIE CAS CSCD 1995年第1期47-50,共4页
Cytokinesis-block micronucleus method is used to evaluate the radiosensitivity of a nasopharyngeal carcinoma cell line (CNE-1) and biopsies obtained from 31 patients with nasopharyngeal carcinoma. The number of micro... Cytokinesis-block micronucleus method is used to evaluate the radiosensitivity of a nasopharyngeal carcinoma cell line (CNE-1) and biopsies obtained from 31 patients with nasopharyngeal carcinoma. The number of micronuclei increases with theradiation dose. A good correlation was found between the radiosensitivity determined by the micro-nucleus assay and that measured by the colony-forming assay in CNE-1 cell line (r=-0.998). Moreover, the results of micronucleus assay for tumor cells from biopsies of patients with primary carcinoma look promising for the prediction of tumor radiosensitivity. These results are encouraging but fleed to be confirmed with a larger number of patients with a longer follow-up. 展开更多
关键词 Micronucleus assay RADIOSENSITIVITY Nasopharyngeal carcinoma
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Comparison of efficacy between two boost treatments in residual tumor of nasopharyngeal carcinoma after radical radiotherapy
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作者 Fan Zhao Oi Wang +1 位作者 Yanliang Sun Xinmei Luo 《The Chinese-German Journal of Clinical Oncology》 CAS 2007年第2期204-206,共3页
Objective: To compare the efficacy between stereotactic radiotherapy (SRT) and intracavitary brachytherapy (brachytherapy) in residual tumor of nasopharyngeal carcinoma (NPC) after treating with conventional ex... Objective: To compare the efficacy between stereotactic radiotherapy (SRT) and intracavitary brachytherapy (brachytherapy) in residual tumor of nasopharyngeal carcinoma (NPC) after treating with conventional external beam radiotherapy. Methods: 60 patients with residual tumor of NPC after radical external beam radiotherapy (range 68 to 72 Gy) were randomized into SRT group (27 patients) and brachytherapy group (33 patients). Patients in SRT group received boost treatment of 10-20 Gy, 2-3 fractions, once every other day; patients in brachytherapy group were treated with boost 10-20 Gy, 5 Gy per fraction, twice a week. Results: Efficacy in the near future: in SRT group, the complete recession (CR), partial recession (PR) and no change (NC) rates were 77.8% (21/27), 18.5% (5/27), 3.7% (1/27), respectively and the efficacy rate was 96.3% (CR + PR); in brachytherapy group: the CR, PR and NC rates were 75.8% (25/33), 18.2% (6/33), 6.1% (2/33), respectively and the efficacy rate was 93.9% (CR + PR). The efficacy rates of the above two groups were compared (x^2 = 0.032, P 〉 0.05). Long term efficacy: in SRT group, 1-year and 3-year survival rates were 96.3%, 66.5% respectively and the median live time was 48 months; in brachytherapy group: 1-year and 3-year survival rates were 93.9%, 60.2% respectively and the median live time was 46 months. The survival rates of two groups were compared (x^2 = 0.172, P 〉 0.05). Conclusion: Both boost techniques of SRT and brachytherapy had elevated efficacy in patients with residual tumor of NPC and there was no obvious difference between the efficacy of the near and long term in SRT and brachytherapy group. 展开更多
关键词 nasopharyngeal neoplasms / radiotherapy EFFICACY
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Detection of Epstein-Barr virus DNA in plasma/serum:a useful serological indicator for diagnosis of nasopharyngeal carcinoma 被引量:1
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作者 买世娟 宗永生 +2 位作者 张敏 钟碧玲 林素暇 《Chinese Medical Journal》 SCIE CAS CSCD 2002年第12期1895-1897,157,共3页
OBJECTIVE: To compare the detection rates of Epstein-Barr virus (EBV) DNA in the serum/plasma between apparently healthy adults (AHAs) and nasopharyngeal carcinoma (NPC) patients in attempt to evaluate the efficiency ... OBJECTIVE: To compare the detection rates of Epstein-Barr virus (EBV) DNA in the serum/plasma between apparently healthy adults (AHAs) and nasopharyngeal carcinoma (NPC) patients in attempt to evaluate the efficiency of EBV DNA assay for serodiagnosis of NPC. METHODS: The plasma and serum were obtained from 58 AHAs and 66 untreated NPC patients. EBV DNA W-fragment was detected using nested ploymerase chain reaction (PCR). Immunoenzymatic assay for titration of IgA-VCA was also adopted. RESULTS: EBV DNA detection rate (84.85%) in the plasma/serum of 66 NPC patients was significantly higher than that (10.34%) in 58 AHAs. The sensitivity of plasma/serum EBV DNA assay (0.8485) was higher than that (0.8030) of titrating IgA-VCA (positive criterion >/= 1:40) though the specificities of these two tests were the same (0.8966). The correct rate, predictive value of a positive test, and Odds ratio of dual positivity (0.8387, 0.9792 and 141.0, respectively) were higher than those of single positivity either to plasma/serum EBV assay (0.5242, 0.7333 and 1.1423, respectively) or to IgA-VCA >/= 1:40 test (0.4839, 0.5385 and 1.0480, respectively). CONCLUSION: The EBV DNA detection in the plasma/serum using nested PCR may be a useful indicator for serodiagnosis of NPC. 展开更多
关键词 Antigens Viral DNA Viral Herpesvirus 4 Human Humans Immunoglobulin A Nasopharyngeal Neoplasms Research Support Non-U.S. Gov't Serologic Tests
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