患者,男,18岁,因纳差、双下肢乏力、肌肉酸痛1 d于2014年9月16日入院。既往无鼻炎病史,家族中无遗传性疾病史。体格检查示体温（T）37.0℃,血压（BP）100/80 mm Hg,神清,精神可,轮椅入病房,查体合作;双下肢肌力2级,双上肢肌张力正常,双...患者,男,18岁,因纳差、双下肢乏力、肌肉酸痛1 d于2014年9月16日入院。既往无鼻炎病史,家族中无遗传性疾病史。体格检查示体温（T）37.0℃,血压（BP）100/80 mm Hg,神清,精神可,轮椅入病房,查体合作;双下肢肌力2级,双上肢肌张力正常,双下肢肌张力减低;双下肢肌肉压痛,生理反射减弱,双上肢生理反射存在,四肢病理反射未引出;余无异常。展开更多
Objective: To examine the role of recombinant adenovirus vector containing CTLA4Ig gene(Ad-CTLA4Ig) in the treatment of induced allergic rhinitis in mice.Methods: Allergic rhinitis was induced by sensitizing and chall...Objective: To examine the role of recombinant adenovirus vector containing CTLA4Ig gene(Ad-CTLA4Ig) in the treatment of induced allergic rhinitis in mice.Methods: Allergic rhinitis was induced by sensitizing and challenging with ovalbumin(OVA).Ad-CTLA4Ig was intraperitoneally injected 30 min before OVA challenge.Adenovirus vector without inserted CTLA4Ig cDNA served as the control.The symptoms and morphological changes of nasal mucosa of each group were observed, and the serum levels of IgE against OVA were detected with ELISA.Results: There were no obvious symptoms and pathological changes in Ad-CTLA4Ig treated group, in which the serum OVA-specific IgE levels were significantly lower than that in control groups(P< 0.05).Conclusion: Ad-CTLA4Ig prevents and treats allergic rhinitis of mice,implying the possibility of the usage of Ad-CTLA4Ig against allergic rhinitis in clinic in future.展开更多
Objective: To evaluate the effects of different surgical procedures on allergic fungal sinusitis. Methods: Thirty-one patients with allergic fungal sinusitis under endoscopic sinus surgery(24 cases) and Caldwell-Luc o...Objective: To evaluate the effects of different surgical procedures on allergic fungal sinusitis. Methods: Thirty-one patients with allergic fungal sinusitis under endoscopic sinus surgery(24 cases) and Caldwell-Luc operation(7 cases) after medical treatment were investigated. Results: No complication was observed during one year's follow-up. No patient who conducted endoscopic sinus surgery reacurred and 3 patients who received traditional surgery required a second operation. Conclusion: This confirmed endoscopic sinus surgery provided a mini-traumatic and effective treatment. Combined treatment of surgical and medical ways was useful to the development of allergic fungal sinusitis.展开更多
Although the PHMG （polyhexamethylene guanidine） and other oligomer guanidines are known as highly efficient biocides against a broad spectrum of microorganisms and eukaryotic cells, the cell protection by PHMG deriv...Although the PHMG （polyhexamethylene guanidine） and other oligomer guanidines are known as highly efficient biocides against a broad spectrum of microorganisms and eukaryotic cells, the cell protection by PHMG derivatives has been established firstly in this study. The antiviral protection was also exhibited after 15 min pretreatment of different cell cultures with low-concentration of PHMG salts. Monolayers of the continuous bovine tracheal cells culture （TCC） and primary culture of chicken embryo fibroblasts （FCE） were treated with aqueous solutions of PHMG chloride salts or PHMG succinate. The molecules of PHMG polycation adhered to the plasma membrane of the cells tested as they were treated with PHMG for 15-30 min. The viral material was added to the cell cultures after the wash-out carried out twice to rid of unbound PHMG. The viruses of Equine herpesvirus type 1, Rhinotracheitis infectious bovine and Equine infectious anemia virus were used. The protective effect from the cytopathic action of herpes and retroviruses was exhibited after 15 min pretreatment of cell monolayer with PHMG chloride at the TCC concentrations of 10^-3 - 10^-2% and FCE concentrations of 10^-5 - 10^-4%. The unique antiviral properties of PHMG salts represented in our research had never been shown before.展开更多
文摘患者,男,18岁,因纳差、双下肢乏力、肌肉酸痛1 d于2014年9月16日入院。既往无鼻炎病史,家族中无遗传性疾病史。体格检查示体温（T）37.0℃,血压（BP）100/80 mm Hg,神清,精神可,轮椅入病房,查体合作;双下肢肌力2级,双上肢肌张力正常,双下肢肌张力减低;双下肢肌肉压痛,生理反射减弱,双上肢生理反射存在,四肢病理反射未引出;余无异常。
基金Supported by the National Natural Science Foundation of China(No.39993430-2)Key Project of Ministry of Science and Techology(No.96-920-20-10)+1 种基金Academician Foundation of Chongqing (No.96-901-05-18)Tackling Key Project of 1999(No.1999-6-40)
文摘Objective: To examine the role of recombinant adenovirus vector containing CTLA4Ig gene(Ad-CTLA4Ig) in the treatment of induced allergic rhinitis in mice.Methods: Allergic rhinitis was induced by sensitizing and challenging with ovalbumin(OVA).Ad-CTLA4Ig was intraperitoneally injected 30 min before OVA challenge.Adenovirus vector without inserted CTLA4Ig cDNA served as the control.The symptoms and morphological changes of nasal mucosa of each group were observed, and the serum levels of IgE against OVA were detected with ELISA.Results: There were no obvious symptoms and pathological changes in Ad-CTLA4Ig treated group, in which the serum OVA-specific IgE levels were significantly lower than that in control groups(P< 0.05).Conclusion: Ad-CTLA4Ig prevents and treats allergic rhinitis of mice,implying the possibility of the usage of Ad-CTLA4Ig against allergic rhinitis in clinic in future.
文摘Objective: To evaluate the effects of different surgical procedures on allergic fungal sinusitis. Methods: Thirty-one patients with allergic fungal sinusitis under endoscopic sinus surgery(24 cases) and Caldwell-Luc operation(7 cases) after medical treatment were investigated. Results: No complication was observed during one year's follow-up. No patient who conducted endoscopic sinus surgery reacurred and 3 patients who received traditional surgery required a second operation. Conclusion: This confirmed endoscopic sinus surgery provided a mini-traumatic and effective treatment. Combined treatment of surgical and medical ways was useful to the development of allergic fungal sinusitis.
文摘Although the PHMG （polyhexamethylene guanidine） and other oligomer guanidines are known as highly efficient biocides against a broad spectrum of microorganisms and eukaryotic cells, the cell protection by PHMG derivatives has been established firstly in this study. The antiviral protection was also exhibited after 15 min pretreatment of different cell cultures with low-concentration of PHMG salts. Monolayers of the continuous bovine tracheal cells culture （TCC） and primary culture of chicken embryo fibroblasts （FCE） were treated with aqueous solutions of PHMG chloride salts or PHMG succinate. The molecules of PHMG polycation adhered to the plasma membrane of the cells tested as they were treated with PHMG for 15-30 min. The viral material was added to the cell cultures after the wash-out carried out twice to rid of unbound PHMG. The viruses of Equine herpesvirus type 1, Rhinotracheitis infectious bovine and Equine infectious anemia virus were used. The protective effect from the cytopathic action of herpes and retroviruses was exhibited after 15 min pretreatment of cell monolayer with PHMG chloride at the TCC concentrations of 10^-3 - 10^-2% and FCE concentrations of 10^-5 - 10^-4%. The unique antiviral properties of PHMG salts represented in our research had never been shown before.
