Orah is a hybrid of "Temple" tangor and "Dancy" tangerine, and due to its excellent performance in cultivation in Wuming of Guangxi Province for three years, it has been popularized in Guangxi, Chongqing, Sichuan ...Orah is a hybrid of "Temple" tangor and "Dancy" tangerine, and due to its excellent performance in cultivation in Wuming of Guangxi Province for three years, it has been popularized in Guangxi, Chongqing, Sichuan and Yunnan rapidly, with a planting area over 130 000 hm^2. However, there also have been some problems existing in practical production, such as leaf yellowing and canker. The causes for leaf yellowing mainly include the physiologic type and the diseases and pests type. This paper analyzed the causes and put forwards corresponding countermeasures.展开更多
Four diterpenoids, including a new ent-kaurane diterpene (1), were isolated from the rhizome of Aralia fargesii Franch. On the basis of chemical and spectral evidence (IR, EI-MS, HREI-MS, H-1-NMR, C-13-NMR and HMQC), ...Four diterpenoids, including a new ent-kaurane diterpene (1), were isolated from the rhizome of Aralia fargesii Franch. On the basis of chemical and spectral evidence (IR, EI-MS, HREI-MS, H-1-NMR, C-13-NMR and HMQC), the structure of compound 1 was established to be 17-acetoxy-16alpha-ent-kauran-19-oic acid The other three known compounds were identified as ent-pimera-8(14) 15-dien-19-oic acid (2), 16alpha-hydroxy-( -)-kauran-19-oic acid (3) and 16alpha-17-dihydroxy-ent-kauran-19-oic acid (4). The three known diterpenoids were obtained from this plant for the first time.展开更多
[Objective] The aim was to explore response characters of NDVI of different types of vegetation to climate change. [Method] Based on NDVI data acquired by SPOT/VGT and meteorological data of five meteorological statio...[Objective] The aim was to explore response characters of NDVI of different types of vegetation to climate change. [Method] Based on NDVI data acquired by SPOT/VGT and meteorological data of five meteorological stations during 19982011, the change trend of NDVI and the relevant correlation with meteorological factors were analyzed in the research area. [Result] NDVI of different types of vegetation in Zhalong wetland kept increasing, especially after 2004. Of the vegetations, NDVI was of significant positive correlation with average temperature, average minimum temperature, average maximum temperature, rainfall and average relative humidity in a ten-day period (P 0.01). In addition, NDVI responses are of different characters to meteorological factors. The responses of NDVI of vegetations were of lag phase to meteorological factors in Zhalong wetland. Specifically, the lag phase of ten-day average temperature and ten-day average minimum temperature was 10-20 d; the lag phase of ten-day average maximum temperature was 20 -30 d; the lag phase of ten-day rainfall was 20 d; the lag phase of ten-day averager relative humidity was 0-10 d. [Conclusion] The research provides references for further exploration of vegetations’ responses to climate change and formulation of vegetation protection and utilization under background of climate change.展开更多
[Objective] This study was to obseve the flower bud differentiation process of Gentiana triflora Pall., and to clarify the flower bud development law and the correlation between flower buds differentiation and externa...[Objective] This study was to obseve the flower bud differentiation process of Gentiana triflora Pall., and to clarify the flower bud development law and the correlation between flower buds differentiation and external morphology. [Method] The paraffin section method was used to observe the flower bud of Gentiana triflora un- der microscope. [Result] The flower bud differentiation process of Gentiana triflora could be divided into six stages: the undifferentiated stage, initial differentiation stage, sepal primordia differentiation stage, petal primordia differentiation stage, stamen pri- mordia differentiation stage and the pistil primordia differentiation stage. The flower bud differentiation started from early May, when the plant had 10-11 stem nodes, and completed in early June, lasted about 40 d. The effect of plant height on flower bud differentiation was not significant. [Conclusion] The study provided theoretical basis for the high-yield cultivation, variety selection and flowering regulation of Gentiana triflora.展开更多
Five forest landscapes in the maps of forest distribution in Heilongjiang Province in 1896 and 1986 were digitized and analyzed through GIS software. The results show that during the 90 years the total patch areas for...Five forest landscapes in the maps of forest distribution in Heilongjiang Province in 1896 and 1986 were digitized and analyzed through GIS software. The results show that during the 90 years the total patch areas for Picea and Abies forest, Pinus sylvestris var. mongolica Litvin forest and Pinus koraiensis Sieb. et Zucc. forest had decreased about 87%, 40% and 84%, respectively. However, the total patch areas for broad leaved forest at the north of 51°N area had increased about 500%. The north frontier of broad leaved forest had moved towards northwest about 290 km. The fractal dimensions of Picea and Abies forest and mixed coniferous and broad leaved forest in 1986 were less than theirs in 1896, respectively. However, the fractal dimension of Pinus synvestris forest, Pinus koraiensis and broad leaved forest were more than theirs in 1896, respectively. Picea and Abies forest, Pinus synvestris forest, Pinus koraiensis and broad leaved forest became more fragmented. The transition percentages of Pinus sylvestris forest, Picea and Abies forest, Pinus koraiensis forest to themselves were less than the percentages to the mixed coniferous and broad leaved forest, respectively.展开更多
With the help of GIS tool of ARC/INFO, ARCVIEW and FRAGSTATS, the map of forest resource distribution of Heilongjiang Province was analyzed in 1896, 1949 and 1981. Using total area, mean patch size, patch density, coe...With the help of GIS tool of ARC/INFO, ARCVIEW and FRAGSTATS, the map of forest resource distribution of Heilongjiang Province was analyzed in 1896, 1949 and 1981. Using total area, mean patch size, patch density, coefficient of patch size variation, mean patch fractal dimension and mean shape index, we studied the change of forest landscape pattern and the change of each patch types in this region. As a result, the total area of forest landscape and mean patch size decreased sharply, the quantity and density of patches increased, the juxtaposition of patches weakened, the shape of patch tended to become regular, and the border of patch simplified. All these showed that the forest landscape of this area tended to fragment gradually, and the fragment of Korean pine forest is the severest. The diversity of whole forest landscape and the evenness of landscape types distribution reduced gradually. Human impact, instead of climate change and forest community succession, is the most important reason for such dramatic changes.展开更多
AIM: To investigate the effect of herbal compound 861 (Cpd861) on the transforming growth factor-β1 (TGFβ1)/ activin receptor-like kinase 1 (ALK1, type Ⅰ receptor) signaling-pathway-related gene expression in the L...AIM: To investigate the effect of herbal compound 861 (Cpd861) on the transforming growth factor-β1 (TGFβ1)/ activin receptor-like kinase 1 (ALK1, type Ⅰ receptor) signaling-pathway-related gene expression in the LX-2 cell line, and the inhibitory mechanism of Cpd861 on the activation of LX-2 cells. METHODS: LX-2 cells were treated with TGFβ1 (5 ng/mL) Cpd861 (0.1 mg/mL), TGFβ1 (5 ng/mL) plus Cpd861 (5 ng/mL) for 24 h to investigate the effect of Cpd861 on the TGFβ1/ALK1 pathway. Real-time PCR was performed to examine the expression of α-SMA (α-smooth muscle actin), ALK1, Id1 (inhibitor of differentiation 1). Western blotting was carried out to measure the levels of α-SMA and phosphorylated Smad1, and immunocytochemical analysis for the expression of α-SMA. RESULTS: In LX-2 cells, TGFβ1/ALK1-pathway-related gene expression could be stimulated by TGFβ1, which led to excessive activation of the cells. Cpd861 decreased the activation of LX-2 cells by reducing the expression of α-SMA mRNA and protein expression. This effect was related to inhibition of the above TGFβ1/ALK1-pathway- related expression of genes such as Id1 and ALK1, and phosphorylation of Smad1 in LX-2 cells, even with TGFβ1 co-treatment for 24 h. CONCLUSION: Cpd861 can restrain the activation of LX-2 cells by inhibiting the TGFβ1/ALK1/Smad1 pathway.展开更多
Gracilaria lemaneiformis Bory is an economically important alga that is primarily used for agar production.Although tetraspores are ideal seeds for the cultivation of G.lemaneiformis,the most popular culture method is...Gracilaria lemaneiformis Bory is an economically important alga that is primarily used for agar production.Although tetraspores are ideal seeds for the cultivation of G.lemaneiformis,the most popular culture method is currently based on vegetative fragments,which is labor-intensive and time-consuming.In this study,we optimized the conditions for tetraspore release and evaluated the photosynthetic activities of different colonies formed from the branches of G.lemaneiformis using a PAM(pulse-amplitude-modulated) measuring system.The results showed that variations in temperature and salinityhad significant effects on tetraspore yield.However,variations in the photon flux density(from 15 μmol m-2 s-1 to 480 μmol m-2 s-1) had no apparent effect on tetraspore yield.Moreover,the PAM-parameters Y(I),Y(Ⅱ),ETR(I),ETR(Ⅱ) and Fv/Fm of colonies formed from different branches showed the same trend:parameter values of first generation branches>second generation branches>third generation branches.These results suggest that the photosynthetic activities of different colonies of branches changed with the same trend.Furthermore,photosynthesis in G.lemaneiformis was found to be involved in vegetative reproduction and tetraspore formation.Finally,the first generation branches grew slowly,but accumulated organic compounds to form large numbers of tetraspores.Taken together,these results showed that the first generation branches are ideal materials for the release of tetraspores.展开更多
Abstract The digestive enzyme activity and mRNA level of trypsin during the embryonic development of Cherax quadricarinatus were analyzed using biochemical and Fluorogenic Quantitative PCR (FQ-PCR) methods. The resu...Abstract The digestive enzyme activity and mRNA level of trypsin during the embryonic development of Cherax quadricarinatus were analyzed using biochemical and Fluorogenic Quantitative PCR (FQ-PCR) methods. The results show that the activities of trypsin and chymotrypsin had two different change patterns. Trypsin specific activity increased rapidly in the early stages of development and still remained high in preparation for the hatch stage. However, chymotrypsin activity peaked in stage 4 of embryonic development and decreased significantly in the last stage. The mRNA level of trypsin was elevated in all stages and two peak values were observed in stages 2 and 5 respectively. The results indicate that trypsin is very important for the utilization of the yolk during embryonic development and for the assimilation of dietary protein for larvae. The gene of trypsin is probably regulated at transcriptional level. The mRNA levels of trypsin can reflect not only trypsin activity, but also the regulatory mechanism for expression of trypsin gene to a certain degree.展开更多
In the Sanjiang Plain,Northeast China,the natural wetland is undergoing a rapid conversion into agricultural land,which has resulted in drastic ecological changes in the region. To investigate the effects of different...In the Sanjiang Plain,Northeast China,the natural wetland is undergoing a rapid conversion into agricultural land,which has resulted in drastic ecological changes in the region. To investigate the effects of different land uses on soil labile organic carbon,soils of Calamagrostis angustifolia wetland,Carex lasiocarpa wetland,dry farmland,paddy field,forest land and abandoned cultivated land were collected for measuring the contents of soil microbial biomass carbon (MBC),dissolved organic carbon (DOC),readily oxidizable carbon (ROC) and carbohydrate carbon (CHC). The results show that soil organic carbon contents follow the order: Carex lasiocarpa wetland>Calamagrostis angustifolia wetland>forest land>paddy field>dry farmland. The contents of MBC and DOC in Calamagrostis angustifolia and Carex lasiocarpa wetlands are significantly higher than those in other land use types. The contents of CHC and ROC are the highest in Calamagrostis angustifolia wetland and the lowest in dry farmland. The contents of all the labile organic carbon increase along with the years of abandonment of cultivated land. The ratios of MBC,DOC and ROC to SOC also follow the order: Carex lasiocarpa wetland>Calamagrostis angustifolia wetland>forest land>paddy field>dry farmland,while the ratio of CHC to SOC is paddy field>forest field>Carex lasiocarpa wetland>Carex lasiocarpa wetland>dry farmland. When natural wetlands were cultivated,the activity of soil organic carbon tends to reduce in some extent due to the disappearance of heterotrophic environment and the reduction of vegetation residue. Thus,the abandonment of cultivated land is an effective way for restoring soil organic carbon.展开更多
The Jiayin area along the Heilongjiang River in Northeast China has attracted stratigraphic and sedimentological interests recently because the Cretaceous--Tertiary (K/T) boundary should be located in this non-marine ...The Jiayin area along the Heilongjiang River in Northeast China has attracted stratigraphic and sedimentological interests recently because the Cretaceous--Tertiary (K/T) boundary should be located in this non-marine succession.Dinosaur remains disappear suddenly at the upper part of the Maastrichtian,whereas plants gradually evolved throughout the succession.Analysis of sedimentary facies has been carried out and paleo-environments through Upper Cretaceous to lower Paleocene sections are reconstructed.Fluvial to lacustrine sedimentary environments are inferred from the facies.Several debris flow events have been identified,and it is in these deposits that the dinosaur fossils are concentrated.展开更多
The effect of starvation on physiological and biochemical indicators of body, muscle and hepatopancreas were investigated for juvenile red swamp crawfish (Procambarus clarkii), and the results showed the effect was ...The effect of starvation on physiological and biochemical indicators of body, muscle and hepatopancreas were investigated for juvenile red swamp crawfish (Procambarus clarkii), and the results showed the effect was obvious. In a 70-day starvation experiment, mean body weight, exuvial rate, abdominal muscle ratio and hepatosomatic index decreased significantly. The percentage of protein, fat, ash and moisture were also significantly affected. The protein content decreased sharply at Day 30 onward and the percentage of fat decreased rapidly from Day 50 and on. The percentages of ash and moisture started to increase significantly at Day 40 and 30 respectively. After the starvation experiment, the hepatopancreatic total and neutral lipid content decreased slightly from 23.90% to 20.39% and 73.75% to 70.35%, while the hepatopancreatic polar lipid content increased from 23.81% to 27.32%. On the other hand, all the muscular total, neutral and polar lipid contents, and the muscular fatty acid compositions did not change significantly except for the hepatopancreatic fatty acid composition. The relative percentages of 14:0, 16:0, 16:1n-7 and 18:1n-9 were decreased by 55.17%, 11.11%, 8.5% and 6.70% from the initial values, and the relative percentages of 18:2n-6, 18:3n-3, 20:4n-6, 20:5n-3 and 22:6n-3 increased obviously from 5.2%, 3.9%,4, 2.4%, 10.6%, and 13.7% to 7.1%, 5.0%, 2.8%, 11.5%, and 14.4% respectively. Preference of utilization of fatty acids from hepatopancreas during starvation was as follows: 14:0〉16:0〉16:1n-7〉18:1n-9, and the conservation of highly unsaturated fatty acids was in the order of: 18:2n-6 〉18:3n-3〉20:4n-6〉20:5n-3〉22:6n-3.展开更多
Thermal expansion characteristics of semimetal nylon composites (nylon 1010 incorporated with metal oxides) were analyzed with thermal expansion instrument. The changes of composite weight after being heated and the h...Thermal expansion characteristics of semimetal nylon composites (nylon 1010 incorporated with metal oxides) were analyzed with thermal expansion instrument. The changes of composite weight after being heated and the heat absorption and release of the composites were analyzed by carrying out TG-DSC experiments. Experimental results show that the average thermal expansion coefficient of the composites rises as the average diameter of the metal oxides decrease from room temperature to 160 ℃. Thermal dynamics and physical properties of the nylon composites change with the addition of the oxides; the crystallization temperature rises from 180 ℃ of pure nylon to 190 ℃ (maximum) and the melting point of the oxide composites also increases continuously with the addition of the oxides. The water content of the oxide/nylon composite is related to the kind and content of the oxide. The water content reaches its maximum when the content of oxide is 10%, and the 10% Al2O3/nylon composite has a water absorption ratio up to 1%.展开更多
Lower Silurian Longmaxi Shale(SLS) in southeastern Sichuan Basin, China, was analyzed for major and selected trace elements, and their provenance, intensity of palaeoweathering of the source rocks were analyzed based ...Lower Silurian Longmaxi Shale(SLS) in southeastern Sichuan Basin, China, was analyzed for major and selected trace elements, and their provenance, intensity of palaeoweathering of the source rocks were analyzed based on these elements. The results show that SiO_2, Al_2O_3 and Fe_2O_3, are dominant major elements with average contents of 60.59%, 15.91% and 5.87% in Upper Silurian Longmaxi Shale(USLS), and 65.14%, 13.24% and 4.68% in Lower Silurian Longmaxi Shale(LSLS). The TiO_2-Zr plot, Hf(ppm) versus La/Th discriminant diagram, and abundance of Cr and Ni suggest a dominantly felsic source for the Longmaxi sediments. Average chemical index of alteration(CIA), plagioclase index of alteration(PIA) values(64.05% and 72.86%, respectively) imply low-degree chemical weathering of the source material in early Longmaxi time, and average CIA, PIA values(68.44% and 80.35%, respectively) imply moderate chemical weathering of the source material in late Longmaxi time.展开更多
In order to determine the area for oil and gas exploration in China’s north Sichuan basin,we have divided the time during which the Longmenshan foreland basin was formed into five periods,based on the sedimentary res...In order to determine the area for oil and gas exploration in China’s north Sichuan basin,we have divided the time during which the Longmenshan foreland basin was formed into five periods,based on the sedimentary response relationship of the foreland basin to structural evolution: 1) a late Triassic Noric period;2) an early-Middle Jurassic period;3) a late Jurassic to early Cretaceous period;4) a late Cretaceous to Paleogene-Neogene period and 5) the Quaternary period. As well,we analyzed the sedimentary environment and lithologic features of every basin-forming period. The results show that there are several favorable source-reservoir-cap assemblages in our study area,making it a major region for future oil and gas exploration in China’s northern Sichuan basin.展开更多
Present article is on Russian Far East carnivorous dinosaur paleoecology.Described summary is based on detailed study and comparison of isolated shed teeth morphology.
Recent geological and paleontological exploration in the Indus basin of Pakistan allowed the discoveries of numerous remains of non-marine reptiles( titanosaurian sauropod,abelisaurian and noasaurian theropod dinosaur...Recent geological and paleontological exploration in the Indus basin of Pakistan allowed the discoveries of numerous remains of non-marine reptiles( titanosaurian sauropod,abelisaurian and noasaurian theropod dinosaurs),and marine reptiles( crocodiles),flying reptiles( pterosaurs),marine and non-marine mammals,fishes,invertebrates,and plants,especially Pakistan is relatively rich in footprints / trackways in the Mesozoic.These vertebrates of Indo-Pakistan are very significant for paleobiogeographic study due to the present-day connection of this continent with Asia in Northern Hemisphere,whereas during past( Jurassic and pre-Jurassic) it was connected to the Gondwana. The Mesozoic vertebrates show close affinities with Gondwanan landmasses.The Cenozoic vertebrates show Eurasian affinity and migrated from Indo-Pak subcontinent to Eurasia or vice versa via Paleo Indus River systems along Western Indus Suture,after long journey of about 6 000 km the first collision of Indo-Pak subcontinent with Asia occurred at terminal Cretaceous.展开更多
基金Supported by the National Citrus Industry System(CARS-27)Key Project of science and Technology from the Agriculture Department of Guangxi Province(201504,201307)+4 种基金Scientific and Technological Development Project of Nanning City(NC20152302,20142062)Scientific and Technological Project of Wuming County(201501217)Fund of Guangxi Academy of Agricultural Sciences(NCZ2015010,GNK2015JM20,GNK2014YZ36,GNK2015YT51,GNK2016YM47)Central Financial Project of Agriculture Technology Extension in 2015Project for the Construction of Good Fruit Variety Breeding Center in Guangxi~~
文摘Orah is a hybrid of "Temple" tangor and "Dancy" tangerine, and due to its excellent performance in cultivation in Wuming of Guangxi Province for three years, it has been popularized in Guangxi, Chongqing, Sichuan and Yunnan rapidly, with a planting area over 130 000 hm^2. However, there also have been some problems existing in practical production, such as leaf yellowing and canker. The causes for leaf yellowing mainly include the physiologic type and the diseases and pests type. This paper analyzed the causes and put forwards corresponding countermeasures.
文摘Four diterpenoids, including a new ent-kaurane diterpene (1), were isolated from the rhizome of Aralia fargesii Franch. On the basis of chemical and spectral evidence (IR, EI-MS, HREI-MS, H-1-NMR, C-13-NMR and HMQC), the structure of compound 1 was established to be 17-acetoxy-16alpha-ent-kauran-19-oic acid The other three known compounds were identified as ent-pimera-8(14) 15-dien-19-oic acid (2), 16alpha-hydroxy-( -)-kauran-19-oic acid (3) and 16alpha-17-dihydroxy-ent-kauran-19-oic acid (4). The three known diterpenoids were obtained from this plant for the first time.
基金Supported by the Special Fund for Meteorological-Scientific Research in the Public Interest (GYHY201306036)Heilongjiang Science Technology Department Key Program (GZ09C102)~~
文摘[Objective] The aim was to explore response characters of NDVI of different types of vegetation to climate change. [Method] Based on NDVI data acquired by SPOT/VGT and meteorological data of five meteorological stations during 19982011, the change trend of NDVI and the relevant correlation with meteorological factors were analyzed in the research area. [Result] NDVI of different types of vegetation in Zhalong wetland kept increasing, especially after 2004. Of the vegetations, NDVI was of significant positive correlation with average temperature, average minimum temperature, average maximum temperature, rainfall and average relative humidity in a ten-day period (P 0.01). In addition, NDVI responses are of different characters to meteorological factors. The responses of NDVI of vegetations were of lag phase to meteorological factors in Zhalong wetland. Specifically, the lag phase of ten-day average temperature and ten-day average minimum temperature was 10-20 d; the lag phase of ten-day average maximum temperature was 20 -30 d; the lag phase of ten-day rainfall was 20 d; the lag phase of ten-day averager relative humidity was 0-10 d. [Conclusion] The research provides references for further exploration of vegetations’ responses to climate change and formulation of vegetation protection and utilization under background of climate change.
基金Supported by the Youth Fund of Heilongjiang University,China~~
文摘[Objective] This study was to obseve the flower bud differentiation process of Gentiana triflora Pall., and to clarify the flower bud development law and the correlation between flower buds differentiation and external morphology. [Method] The paraffin section method was used to observe the flower bud of Gentiana triflora un- der microscope. [Result] The flower bud differentiation process of Gentiana triflora could be divided into six stages: the undifferentiated stage, initial differentiation stage, sepal primordia differentiation stage, petal primordia differentiation stage, stamen pri- mordia differentiation stage and the pistil primordia differentiation stage. The flower bud differentiation started from early May, when the plant had 10-11 stem nodes, and completed in early June, lasted about 40 d. The effect of plant height on flower bud differentiation was not significant. [Conclusion] The study provided theoretical basis for the high-yield cultivation, variety selection and flowering regulation of Gentiana triflora.
文摘Five forest landscapes in the maps of forest distribution in Heilongjiang Province in 1896 and 1986 were digitized and analyzed through GIS software. The results show that during the 90 years the total patch areas for Picea and Abies forest, Pinus sylvestris var. mongolica Litvin forest and Pinus koraiensis Sieb. et Zucc. forest had decreased about 87%, 40% and 84%, respectively. However, the total patch areas for broad leaved forest at the north of 51°N area had increased about 500%. The north frontier of broad leaved forest had moved towards northwest about 290 km. The fractal dimensions of Picea and Abies forest and mixed coniferous and broad leaved forest in 1986 were less than theirs in 1896, respectively. However, the fractal dimension of Pinus synvestris forest, Pinus koraiensis and broad leaved forest were more than theirs in 1896, respectively. Picea and Abies forest, Pinus synvestris forest, Pinus koraiensis and broad leaved forest became more fragmented. The transition percentages of Pinus sylvestris forest, Picea and Abies forest, Pinus koraiensis forest to themselves were less than the percentages to the mixed coniferous and broad leaved forest, respectively.
基金The research is supported by Study on the interaction of global change and terrestrial ecosystem in eastern China - 39899370 and the Northeast Forestry University research fund.
文摘With the help of GIS tool of ARC/INFO, ARCVIEW and FRAGSTATS, the map of forest resource distribution of Heilongjiang Province was analyzed in 1896, 1949 and 1981. Using total area, mean patch size, patch density, coefficient of patch size variation, mean patch fractal dimension and mean shape index, we studied the change of forest landscape pattern and the change of each patch types in this region. As a result, the total area of forest landscape and mean patch size decreased sharply, the quantity and density of patches increased, the juxtaposition of patches weakened, the shape of patch tended to become regular, and the border of patch simplified. All these showed that the forest landscape of this area tended to fragment gradually, and the fragment of Korean pine forest is the severest. The diversity of whole forest landscape and the evenness of landscape types distribution reduced gradually. Human impact, instead of climate change and forest community succession, is the most important reason for such dramatic changes.
文摘AIM: To investigate the effect of herbal compound 861 (Cpd861) on the transforming growth factor-β1 (TGFβ1)/ activin receptor-like kinase 1 (ALK1, type Ⅰ receptor) signaling-pathway-related gene expression in the LX-2 cell line, and the inhibitory mechanism of Cpd861 on the activation of LX-2 cells. METHODS: LX-2 cells were treated with TGFβ1 (5 ng/mL) Cpd861 (0.1 mg/mL), TGFβ1 (5 ng/mL) plus Cpd861 (5 ng/mL) for 24 h to investigate the effect of Cpd861 on the TGFβ1/ALK1 pathway. Real-time PCR was performed to examine the expression of α-SMA (α-smooth muscle actin), ALK1, Id1 (inhibitor of differentiation 1). Western blotting was carried out to measure the levels of α-SMA and phosphorylated Smad1, and immunocytochemical analysis for the expression of α-SMA. RESULTS: In LX-2 cells, TGFβ1/ALK1-pathway-related gene expression could be stimulated by TGFβ1, which led to excessive activation of the cells. Cpd861 decreased the activation of LX-2 cells by reducing the expression of α-SMA mRNA and protein expression. This effect was related to inhibition of the above TGFβ1/ALK1-pathway- related expression of genes such as Id1 and ALK1, and phosphorylation of Smad1 in LX-2 cells, even with TGFβ1 co-treatment for 24 h. CONCLUSION: Cpd861 can restrain the activation of LX-2 cells by inhibiting the TGFβ1/ALK1/Smad1 pathway.
基金Supported by the National Natural Science Foundation of China(No.30830015)the National Key Technology Research and Development Program of the 11th Five-Year Plan(No.2006BAD09A04)the National High Technology Research and Development Program of China(863Program,Nos.2006AA10A402,2007AA09Z406,2006AA05Z112,2006AA10A413)
文摘Gracilaria lemaneiformis Bory is an economically important alga that is primarily used for agar production.Although tetraspores are ideal seeds for the cultivation of G.lemaneiformis,the most popular culture method is currently based on vegetative fragments,which is labor-intensive and time-consuming.In this study,we optimized the conditions for tetraspore release and evaluated the photosynthetic activities of different colonies formed from the branches of G.lemaneiformis using a PAM(pulse-amplitude-modulated) measuring system.The results showed that variations in temperature and salinityhad significant effects on tetraspore yield.However,variations in the photon flux density(from 15 μmol m-2 s-1 to 480 μmol m-2 s-1) had no apparent effect on tetraspore yield.Moreover,the PAM-parameters Y(I),Y(Ⅱ),ETR(I),ETR(Ⅱ) and Fv/Fm of colonies formed from different branches showed the same trend:parameter values of first generation branches>second generation branches>third generation branches.These results suggest that the photosynthetic activities of different colonies of branches changed with the same trend.Furthermore,photosynthesis in G.lemaneiformis was found to be involved in vegetative reproduction and tetraspore formation.Finally,the first generation branches grew slowly,but accumulated organic compounds to form large numbers of tetraspores.Taken together,these results showed that the first generation branches are ideal materials for the release of tetraspores.
基金Supported by the NNSF of China (No.30670227)hanghai Agricultural Science & Technology Key Grant [6-1(2006)].
文摘Abstract The digestive enzyme activity and mRNA level of trypsin during the embryonic development of Cherax quadricarinatus were analyzed using biochemical and Fluorogenic Quantitative PCR (FQ-PCR) methods. The results show that the activities of trypsin and chymotrypsin had two different change patterns. Trypsin specific activity increased rapidly in the early stages of development and still remained high in preparation for the hatch stage. However, chymotrypsin activity peaked in stage 4 of embryonic development and decreased significantly in the last stage. The mRNA level of trypsin was elevated in all stages and two peak values were observed in stages 2 and 5 respectively. The results indicate that trypsin is very important for the utilization of the yolk during embryonic development and for the assimilation of dietary protein for larvae. The gene of trypsin is probably regulated at transcriptional level. The mRNA levels of trypsin can reflect not only trypsin activity, but also the regulatory mechanism for expression of trypsin gene to a certain degree.
基金Under the auspices of National Natural Science Foundation of China (No. 30470340)
文摘In the Sanjiang Plain,Northeast China,the natural wetland is undergoing a rapid conversion into agricultural land,which has resulted in drastic ecological changes in the region. To investigate the effects of different land uses on soil labile organic carbon,soils of Calamagrostis angustifolia wetland,Carex lasiocarpa wetland,dry farmland,paddy field,forest land and abandoned cultivated land were collected for measuring the contents of soil microbial biomass carbon (MBC),dissolved organic carbon (DOC),readily oxidizable carbon (ROC) and carbohydrate carbon (CHC). The results show that soil organic carbon contents follow the order: Carex lasiocarpa wetland>Calamagrostis angustifolia wetland>forest land>paddy field>dry farmland. The contents of MBC and DOC in Calamagrostis angustifolia and Carex lasiocarpa wetlands are significantly higher than those in other land use types. The contents of CHC and ROC are the highest in Calamagrostis angustifolia wetland and the lowest in dry farmland. The contents of all the labile organic carbon increase along with the years of abandonment of cultivated land. The ratios of MBC,DOC and ROC to SOC also follow the order: Carex lasiocarpa wetland>Calamagrostis angustifolia wetland>forest land>paddy field>dry farmland,while the ratio of CHC to SOC is paddy field>forest field>Carex lasiocarpa wetland>Carex lasiocarpa wetland>dry farmland. When natural wetlands were cultivated,the activity of soil organic carbon tends to reduce in some extent due to the disappearance of heterotrophic environment and the reduction of vegetation residue. Thus,the abandonment of cultivated land is an effective way for restoring soil organic carbon.
基金supported by the fund for the Projects 30220130698 and 40842002 of NSFC,Chinathe Project 111 of China (Jilin University)
文摘The Jiayin area along the Heilongjiang River in Northeast China has attracted stratigraphic and sedimentological interests recently because the Cretaceous--Tertiary (K/T) boundary should be located in this non-marine succession.Dinosaur remains disappear suddenly at the upper part of the Maastrichtian,whereas plants gradually evolved throughout the succession.Analysis of sedimentary facies has been carried out and paleo-environments through Upper Cretaceous to lower Paleocene sections are reconstructed.Fluvial to lacustrine sedimentary environments are inferred from the facies.Several debris flow events have been identified,and it is in these deposits that the dinosaur fossils are concentrated.
基金Supported by the Scientific Fund of Chinese Postdoctor (No. 2002032195).
文摘The effect of starvation on physiological and biochemical indicators of body, muscle and hepatopancreas were investigated for juvenile red swamp crawfish (Procambarus clarkii), and the results showed the effect was obvious. In a 70-day starvation experiment, mean body weight, exuvial rate, abdominal muscle ratio and hepatosomatic index decreased significantly. The percentage of protein, fat, ash and moisture were also significantly affected. The protein content decreased sharply at Day 30 onward and the percentage of fat decreased rapidly from Day 50 and on. The percentages of ash and moisture started to increase significantly at Day 40 and 30 respectively. After the starvation experiment, the hepatopancreatic total and neutral lipid content decreased slightly from 23.90% to 20.39% and 73.75% to 70.35%, while the hepatopancreatic polar lipid content increased from 23.81% to 27.32%. On the other hand, all the muscular total, neutral and polar lipid contents, and the muscular fatty acid compositions did not change significantly except for the hepatopancreatic fatty acid composition. The relative percentages of 14:0, 16:0, 16:1n-7 and 18:1n-9 were decreased by 55.17%, 11.11%, 8.5% and 6.70% from the initial values, and the relative percentages of 18:2n-6, 18:3n-3, 20:4n-6, 20:5n-3 and 22:6n-3 increased obviously from 5.2%, 3.9%,4, 2.4%, 10.6%, and 13.7% to 7.1%, 5.0%, 2.8%, 11.5%, and 14.4% respectively. Preference of utilization of fatty acids from hepatopancreas during starvation was as follows: 14:0〉16:0〉16:1n-7〉18:1n-9, and the conservation of highly unsaturated fatty acids was in the order of: 18:2n-6 〉18:3n-3〉20:4n-6〉20:5n-3〉22:6n-3.
基金Projects 50225519 supported by the National Natural Science Foundation of China and 0E4458the Youth Science Foundation of China University of Mining & Technology
文摘Thermal expansion characteristics of semimetal nylon composites (nylon 1010 incorporated with metal oxides) were analyzed with thermal expansion instrument. The changes of composite weight after being heated and the heat absorption and release of the composites were analyzed by carrying out TG-DSC experiments. Experimental results show that the average thermal expansion coefficient of the composites rises as the average diameter of the metal oxides decrease from room temperature to 160 ℃. Thermal dynamics and physical properties of the nylon composites change with the addition of the oxides; the crystallization temperature rises from 180 ℃ of pure nylon to 190 ℃ (maximum) and the melting point of the oxide composites also increases continuously with the addition of the oxides. The water content of the oxide/nylon composite is related to the kind and content of the oxide. The water content reaches its maximum when the content of oxide is 10%, and the 10% Al2O3/nylon composite has a water absorption ratio up to 1%.
基金Project(41302076)supported by the National Natural Science Foundation of ChinaProject(BJ14266)supported by MOST Special Fund from the State Key Laboratory of Continental Dynamics,Northwest University,ChinaProject(2014JQ5191)supported by Natural Science Basic Research Plan of Shaanxi Province,China
文摘Lower Silurian Longmaxi Shale(SLS) in southeastern Sichuan Basin, China, was analyzed for major and selected trace elements, and their provenance, intensity of palaeoweathering of the source rocks were analyzed based on these elements. The results show that SiO_2, Al_2O_3 and Fe_2O_3, are dominant major elements with average contents of 60.59%, 15.91% and 5.87% in Upper Silurian Longmaxi Shale(USLS), and 65.14%, 13.24% and 4.68% in Lower Silurian Longmaxi Shale(LSLS). The TiO_2-Zr plot, Hf(ppm) versus La/Th discriminant diagram, and abundance of Cr and Ni suggest a dominantly felsic source for the Longmaxi sediments. Average chemical index of alteration(CIA), plagioclase index of alteration(PIA) values(64.05% and 72.86%, respectively) imply low-degree chemical weathering of the source material in early Longmaxi time, and average CIA, PIA values(68.44% and 80.35%, respectively) imply moderate chemical weathering of the source material in late Longmaxi time.
基金Projects 40772198 and 50678182 supported by the National Natural Science Foundation of China09-3-094 by the Research Fund for Teaching Reform in Institutes of Higher Learning,Chongqing, China
文摘In order to determine the area for oil and gas exploration in China’s north Sichuan basin,we have divided the time during which the Longmenshan foreland basin was formed into five periods,based on the sedimentary response relationship of the foreland basin to structural evolution: 1) a late Triassic Noric period;2) an early-Middle Jurassic period;3) a late Jurassic to early Cretaceous period;4) a late Cretaceous to Paleogene-Neogene period and 5) the Quaternary period. As well,we analyzed the sedimentary environment and lithologic features of every basin-forming period. The results show that there are several favorable source-reservoir-cap assemblages in our study area,making it a major region for future oil and gas exploration in China’s northern Sichuan basin.
基金supported by the Russian Foundation of Fundamental Research Grants No. 07-05-00168-а and No. 10-05-00151-аsupported by the Key-Lab for Evolution of Past Life and Environment in NE Asia, Ministry of Education, China (Jilin University)
文摘Present article is on Russian Far East carnivorous dinosaur paleoecology.Described summary is based on detailed study and comparison of isolated shed teeth morphology.
文摘Recent geological and paleontological exploration in the Indus basin of Pakistan allowed the discoveries of numerous remains of non-marine reptiles( titanosaurian sauropod,abelisaurian and noasaurian theropod dinosaurs),and marine reptiles( crocodiles),flying reptiles( pterosaurs),marine and non-marine mammals,fishes,invertebrates,and plants,especially Pakistan is relatively rich in footprints / trackways in the Mesozoic.These vertebrates of Indo-Pakistan are very significant for paleobiogeographic study due to the present-day connection of this continent with Asia in Northern Hemisphere,whereas during past( Jurassic and pre-Jurassic) it was connected to the Gondwana. The Mesozoic vertebrates show close affinities with Gondwanan landmasses.The Cenozoic vertebrates show Eurasian affinity and migrated from Indo-Pak subcontinent to Eurasia or vice versa via Paleo Indus River systems along Western Indus Suture,after long journey of about 6 000 km the first collision of Indo-Pak subcontinent with Asia occurred at terminal Cretaceous.