目的基于脑肠轴理论探讨柴胡加龙骨牡蛎汤对癫痫患者胶质纤维酸性蛋白、神经肽Y及S100B的影响。方法纳入2018年1月—2020年1月癫痫患者114例作为观察对象,随机分为两组,对照组57例选用电子生物反馈技术;联合组57例选用柴胡加龙骨牡蛎汤...目的基于脑肠轴理论探讨柴胡加龙骨牡蛎汤对癫痫患者胶质纤维酸性蛋白、神经肽Y及S100B的影响。方法纳入2018年1月—2020年1月癫痫患者114例作为观察对象,随机分为两组,对照组57例选用电子生物反馈技术;联合组57例选用柴胡加龙骨牡蛎汤联合电子生物反馈技术,持续治疗3个月。比较临床疗效及癫痫生活质量评定量表(epilepsy quality of life scale-31,QOLIE-31)、不良反应,治疗前后测定血清胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、神经肽Y(neuropeptide Y,NPY)及S100B水平。结果对照组有效率82.46%(47/57)低于联合组94.74%(54/57),有统计学意义(P<0.05)。与治疗前比,两组治疗后血清GFAP、NPY及S100B水平均降低,而且联合组治疗后血清GFAP、NPY及S100B水平低于对照组,有统计学意义(P<0.05)。对照组综合质量、情绪、总体健康、药物影响、认知功能、社会功能、精力及对发作担忧评分低于联合组,有统计学意义(P<0.05)。两组患者不良反应率分析,无统计学意义(P>0.05)。结论柴胡加龙骨牡蛎汤有疏利肝胆,和解少阳之功,对癫痫患者的疗效显著,可以降低神经细胞因子,提高生活质量,值得推广。展开更多
Objective To explore the effect and mechanism of Chaihu Longgu Muli Decoction(柴胡龙骨牡蛎汤,CHLGMLD)in rats with temporal lobe epilepsy(TLE).Methods A total of 80 Sprague-Dawley(SD)male rats were randomized into cont...Objective To explore the effect and mechanism of Chaihu Longgu Muli Decoction(柴胡龙骨牡蛎汤,CHLGMLD)in rats with temporal lobe epilepsy(TLE).Methods A total of 80 Sprague-Dawley(SD)male rats were randomized into control(CON),model(MOD),carbamazepine(CBZ,0.1 g/kg),CHLGMLD low dose(CHLGMLD-L,12.5 g/kg),and high dose(CHLGMLD-H,25 g/kg)groups,with 16 rats in each group.TLE rat models were established in the four groups with the use of lithium-pilocarpine except for the CON group.After the successful establishment of TLE models,all drugs were administered through gavage,and distilled water was given to rats in the CON and MOD groups for four weeks.The frequency and duration of seizures before and after treatment were recorded for the evaluation of the alleviation degree.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression levels of miR-146a-3p and miR-146a-5p.The expression levels of toll-like receptor 4(TLR4),interleukin-1 receptor-associated kinase 1(IRAK1),tumor necrosis factor(TNF)receptor-associated factor 6(TRAF6),TAK1-binding protein(TAB),nuclear factor-kappa B(NF-κB),and interleukin-1 beta(IL-1β)in hippocampus were tested by immunofluorescence assay.Correlation analysis between the above factors and expressions of miR-146a-3p and miR-146a-5p were performed separately.Results CHLGMLD decreased the frequency(P<0.05)and duration(P<0.01)of seizures in rats.CHLGMLD down-regulated the expression levels of miR-146a-5p and miR-146a-3p(P<0.05),and inhibited the expression levels of TLR4,IRAK1,TRAF6,TAB,NF-κB,and IL-1β(P<0.01).The correlation analysis revealed that the expression levels of TLR4,IRAK1,TRAF6,TAB,NF-κB,and IL-1β were positively correlated with the expression levels of miR-146a-3p and miR-146a-5p detected by qRT-PCR,respectively(P<0.01).Conclusion CHLGMLD can inhibite the TLR4 signaling pathway by lowering the expression levels of miR-146a-3p and miR-146a-5p to alleviate hippocampal dentate gyrus inflammation in TLE rats,thus relieving seizures.展开更多
文摘目的基于脑肠轴理论探讨柴胡加龙骨牡蛎汤对癫痫患者胶质纤维酸性蛋白、神经肽Y及S100B的影响。方法纳入2018年1月—2020年1月癫痫患者114例作为观察对象,随机分为两组,对照组57例选用电子生物反馈技术;联合组57例选用柴胡加龙骨牡蛎汤联合电子生物反馈技术,持续治疗3个月。比较临床疗效及癫痫生活质量评定量表(epilepsy quality of life scale-31,QOLIE-31)、不良反应,治疗前后测定血清胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、神经肽Y(neuropeptide Y,NPY)及S100B水平。结果对照组有效率82.46%(47/57)低于联合组94.74%(54/57),有统计学意义(P<0.05)。与治疗前比,两组治疗后血清GFAP、NPY及S100B水平均降低,而且联合组治疗后血清GFAP、NPY及S100B水平低于对照组,有统计学意义(P<0.05)。对照组综合质量、情绪、总体健康、药物影响、认知功能、社会功能、精力及对发作担忧评分低于联合组,有统计学意义(P<0.05)。两组患者不良反应率分析,无统计学意义(P>0.05)。结论柴胡加龙骨牡蛎汤有疏利肝胆,和解少阳之功,对癫痫患者的疗效显著,可以降低神经细胞因子,提高生活质量,值得推广。
基金National Natural Science Foundation of China(81874429)Natural Science Foundation of Hunan Province(2020JJ5294)+3 种基金Traditional Chinese Medicine Science&Research Project of Hunan Province(202145)Excellent Youth Program of Hunan Education Department(21B0081)Hunan Provincial Administration of Traditional Chinese Medicine(D2022027)Changsha Natural Science Foundation of China(KQ2202255).
文摘Objective To explore the effect and mechanism of Chaihu Longgu Muli Decoction(柴胡龙骨牡蛎汤,CHLGMLD)in rats with temporal lobe epilepsy(TLE).Methods A total of 80 Sprague-Dawley(SD)male rats were randomized into control(CON),model(MOD),carbamazepine(CBZ,0.1 g/kg),CHLGMLD low dose(CHLGMLD-L,12.5 g/kg),and high dose(CHLGMLD-H,25 g/kg)groups,with 16 rats in each group.TLE rat models were established in the four groups with the use of lithium-pilocarpine except for the CON group.After the successful establishment of TLE models,all drugs were administered through gavage,and distilled water was given to rats in the CON and MOD groups for four weeks.The frequency and duration of seizures before and after treatment were recorded for the evaluation of the alleviation degree.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression levels of miR-146a-3p and miR-146a-5p.The expression levels of toll-like receptor 4(TLR4),interleukin-1 receptor-associated kinase 1(IRAK1),tumor necrosis factor(TNF)receptor-associated factor 6(TRAF6),TAK1-binding protein(TAB),nuclear factor-kappa B(NF-κB),and interleukin-1 beta(IL-1β)in hippocampus were tested by immunofluorescence assay.Correlation analysis between the above factors and expressions of miR-146a-3p and miR-146a-5p were performed separately.Results CHLGMLD decreased the frequency(P<0.05)and duration(P<0.01)of seizures in rats.CHLGMLD down-regulated the expression levels of miR-146a-5p and miR-146a-3p(P<0.05),and inhibited the expression levels of TLR4,IRAK1,TRAF6,TAB,NF-κB,and IL-1β(P<0.01).The correlation analysis revealed that the expression levels of TLR4,IRAK1,TRAF6,TAB,NF-κB,and IL-1β were positively correlated with the expression levels of miR-146a-3p and miR-146a-5p detected by qRT-PCR,respectively(P<0.01).Conclusion CHLGMLD can inhibite the TLR4 signaling pathway by lowering the expression levels of miR-146a-3p and miR-146a-5p to alleviate hippocampal dentate gyrus inflammation in TLE rats,thus relieving seizures.
