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龟皮胶原蛋白的提取及分析 被引量:3
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作者 邓燕 印大中 江南 《食品研究与开发》 CAS 北大核心 2006年第6期62-64,共3页
采用胃蛋白酶酶解法从龟皮中提取胶原蛋白,经SDS-PAGE电泳发现酶解后的电泳带型变化可知,用胃蛋白酶酶解,猪皮的酶解速率不如龟皮,龟皮胶原更易降解为小分子肽,易于被身体吸收利用;氨基酸成分分析显示,龟皮胶原相对于猪皮胶原含有更全... 采用胃蛋白酶酶解法从龟皮中提取胶原蛋白,经SDS-PAGE电泳发现酶解后的电泳带型变化可知,用胃蛋白酶酶解,猪皮的酶解速率不如龟皮,龟皮胶原更易降解为小分子肽,易于被身体吸收利用;氨基酸成分分析显示,龟皮胶原相对于猪皮胶原含有更全面的氨基酸和含有高于猪皮胶原的羟脯氨酸,具有更丰富的营养价值。这对于龟皮胶原蛋白的开发利用具有十分重要的意义。 展开更多
关键词 龟皮 胶原蛋白 提取 SDS—PAGE 氨基酸
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龟皮胶原蛋白的提取及结构表征(Ⅰ) 被引量:3
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作者 黄文 印大中 朱泽瑞 《湖北大学学报(自然科学版)》 CAS 北大核心 2010年第4期434-437,共4页
采用酶法从乌龟皮中提取胶原蛋白,用聚丙烯酰胺凝胶电泳(SDS-PAGE)方法检测龟皮胶原蛋白多肽产品分子量的分布,并将龟皮胶原蛋白和Ⅰ型胶原蛋白样品进行液相色谱分析(HPLC),比较其氨基酸成分和含量.SDS-PAGE电泳结果分析表明龟皮胶原为... 采用酶法从乌龟皮中提取胶原蛋白,用聚丙烯酰胺凝胶电泳(SDS-PAGE)方法检测龟皮胶原蛋白多肽产品分子量的分布,并将龟皮胶原蛋白和Ⅰ型胶原蛋白样品进行液相色谱分析(HPLC),比较其氨基酸成分和含量.SDS-PAGE电泳结果分析表明龟皮胶原为Ⅰ型胶原蛋白,分子量约为288ku.龟皮胶原蛋白中,甘氨酸的含量最高,占总量的24.69%;其次是谷氨酸、精氨酸和丙氨酸,分别为9.95%、8.08%和9.31%;天冬氨酸、赖氨酸、丝氨酸、苏氨酸、亮氨酸、苯丙氨酸、异亮氨酸、缬氨酸、酪氨酸等含量在5.58%~1.28%之间;组氨酸和蛋氨酸的含量最低,仅占0.79%和0.09%.由于龟皮胶原蛋白的含量高于Ⅰ型胶原蛋白,且与Ⅰ型胶原蛋白的氨基酸含量差异显著,因此,龟皮胶原蛋白可以作为一种重要的皮胶原进行开发和利用. 展开更多
关键词 龟皮 胶原蛋白 SDS-PAGE 氨基酸
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龟皮提取物对大鼠皮肤中羟脯氨酸的影响 被引量:2
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作者 吴耀松 廖艳阳 朱泽瑞 《临床皮肤科杂志》 CAS CSCD 北大核心 2006年第5期299-300,共2页
关键词 衰老 胶原蛋白 龟皮提取物 大鼠
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水发鱼皮工艺研究 被引量:14
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作者 周婉君 王剑河 +4 位作者 吴燕燕 李来好 郝淑贤 岑剑伟 陈胜军 《食品科学》 EI CAS CSCD 北大核心 2007年第8期233-236,共4页
本实验以罗非鱼片加工中产生的下脚料——鱼皮为原料,研究水发鱼皮加工工艺技术。经过实验筛选出最佳工艺条件为:鱼皮在25℃下,浓度为0.0225g/L的碱液中浸泡6h,达到最佳水发效果,然后在90℃恒温烫漂30s,最后用无菌冰水浸泡至pH7,于4℃... 本实验以罗非鱼片加工中产生的下脚料——鱼皮为原料,研究水发鱼皮加工工艺技术。经过实验筛选出最佳工艺条件为:鱼皮在25℃下,浓度为0.0225g/L的碱液中浸泡6h,达到最佳水发效果,然后在90℃恒温烫漂30s,最后用无菌冰水浸泡至pH7,于4℃下贮藏在30d。在此工艺条件下生产的水发罗非鱼皮,口感爽脆,质量最佳。罗非鱼水发鱼皮工艺技术的研究为罗非鱼皮的开发利用提供了新的途径。 展开更多
关键词 罗非鱼 水发龟皮 工艺技术
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龟嗜皮菌的快速鉴定及其在鳄蜥皮肤病原检测中的应用 被引量:2
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作者 江海英 陈金平 +4 位作者 黄铭威 林立波 李林妙 张秀娟 李官钰 《动物学杂志》 CAS CSCD 北大核心 2021年第1期111-118,共8页
龟嗜皮菌(Austwickia chelonae)感染爬行类、鸟类、哺乳类等,造成野生动物和家养动物患皮肤病甚至死亡,近年来这种病原体在Ⅰ级保护动物鳄蜥(Shinisaurus crocodilurus)的救护种群中暴发。传统的病原检测方法费时耗力。本文基于龟嗜皮... 龟嗜皮菌(Austwickia chelonae)感染爬行类、鸟类、哺乳类等,造成野生动物和家养动物患皮肤病甚至死亡,近年来这种病原体在Ⅰ级保护动物鳄蜥(Shinisaurus crocodilurus)的救护种群中暴发。传统的病原检测方法费时耗力。本文基于龟嗜皮菌全基因组序列开发了特异性高、方便快捷的龟嗜皮菌检测方法,并应用于鳄蜥的皮肤病风险预测。本研究开发的3对检测龟嗜皮菌的特异引物中,AC3引物的使用效果最好。 展开更多
关键词 鉴定 肤病 鳄蜥
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Intravascular Access in Chelonia mydas and Dermochelys coriacea Using the Seldinger Technique U Itrasou nd-Guided
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作者 Gustavo Henrique Pereira Dutra Fálbio Futema +1 位作者 Flávio Augusto Marques dos Santos Cristiane Lassalvia 《Journal of Life Sciences》 2014年第5期385-393,共9页
Strand-ed turtles need fluid parenterally. The jugular access is best for the maintenance and patency of the catheter. The Seldinger technique guided by ultrasound seems to be the safest for catheter insertion. Five j... Strand-ed turtles need fluid parenterally. The jugular access is best for the maintenance and patency of the catheter. The Seldinger technique guided by ultrasound seems to be the safest for catheter insertion. Five juveniles Chelonia mydas with fibropapillomatosis and an adult male leatherback turtle (Dermochelys coriacea) with altered buoyancy were sent to Santos Municipal Aquarium for rehabilitation. Turtles underwent catheterization of the jugular vein using the Seldinger technique with central venous polyurethane catheter monolumen 14 G to Dermochelys and 18 G for Chelonia, guided by ultrasound with 10 MHz transducer catheters were sutured to the skin and animals were subjected to fluid. In all turtles, the catheters were patency and were well established. There were no granulomatous reactions or related infections. The Dermochelys improved clinically after fluid resuscitation, and the catheter was removed one week after. In one Chelonia excision was 21% of its weight in tumors, and the animals received colloid catheter by enabling the mitigation of bleeding due to removal. The use of ultrasonography has enabled monitoring of all access as the patency of the catheter and fluid diffusivity. 展开更多
关键词 Sea turtles CHELONIA Dermochelys ultrassonography Seldinger technique.
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Sea Turtles in Vietnam: On the Edge of Extinction
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作者 Cuong The Chu The Duc Nguyen 《Journal of Life Sciences》 2015年第8期356-361,共6页
Five species of foraging and breeding sea turtles have been identified in Vietnam, they are Green turtle (Chelonia mydas), Loggerhead turtle (Caretta caretta), Olive ridley turtle (Lepidochelys olivacea), Hawksb... Five species of foraging and breeding sea turtles have been identified in Vietnam, they are Green turtle (Chelonia mydas), Loggerhead turtle (Caretta caretta), Olive ridley turtle (Lepidochelys olivacea), Hawksbill turtle (Eretmochelys imbricata) and Leatherback turtle (Dermochelys coriacea). Among them, with the exception of Loggerhead turtle (Caretta caretta), four species have been being lay eggs on the beaches along the country. However, these populations have been strongly suffered by human activities for decades. The number of foraging and breeding Hawksbill and Leatherback sea turtles has dramatically decreased, and Olive Ridley turtle has almost disappeared on their traditional nesting beaches. Beside the reason of harvesting eggs and nesting turtles by catch or by purpose, the developments in coastal areas where sea turtles laying eggs such as in shrimp aquaculture, tourist resort and residential area developments etc., also contributed to this decline of sea turtles. Despite the fact that the recent raising awareness programs on protection and conservation of the sea turtle were very well done with the participation of many organizations and provinces, this important animals still face the risk of extinction in Vietnam. 展开更多
关键词 Sea turtle NESTING foraging threats.
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