One new sesquiterpene lactone, corialactone E （1）, one new neolignan, coriarianeolignan A （2）, together with three known apocarotenoids （3-5） and one known neolignan （6） have been isolated from a CHCI3 extract...One new sesquiterpene lactone, corialactone E （1）, one new neolignan, coriarianeolignan A （2）, together with three known apocarotenoids （3-5） and one known neolignan （6） have been isolated from a CHCI3 extract of the roots of Coriaria nepalensis. The structures including absolute configurations of 1-6 were elucidated through extensive NMR, HR-ESIMS, and CD data analysis. Structurally, compound I possessed novel variations in the structure, including the newly formed ether ring of C-3/O/C-9 and the lactone ring connecting C-13 and C-5. Compound 5 showed cytotoxic activity against SKOV3 （human ovarian cancer） cells with IC50 values of 4.67 μmol/L. In vivo system, compound 3 showed anti-convulsant activity by 34% at the dose of 5 mg/kg.展开更多
Two new compounds. corialins A （1） and B （2） were isolated from Coriaria nepulensis Wall. These new compounds were established as 7-hydroxy-3-[2,3-acetonidc-（3-methylhutane）] coumarin （1） and 3-O-β-D-glucopyr...Two new compounds. corialins A （1） and B （2） were isolated from Coriaria nepulensis Wall. These new compounds were established as 7-hydroxy-3-[2,3-acetonidc-（3-methylhutane）] coumarin （1） and 3-O-β-D-glucopyranosyl-3,4,5-1rihydroxy-1-（3- methyl-2-butenyl）-benzene （2）. on the basis of ID and 2D NMR techniques.展开更多
Four strains with typical morphology of actinomycete genus of Frankia were isolated from root nodules of Coriaria nepalensis. They were shown to nodulate the seedlings of host plant and hybridize with Frankia 16S rRNA...Four strains with typical morphology of actinomycete genus of Frankia were isolated from root nodules of Coriaria nepalensis. They were shown to nodulate the seedlings of host plant and hybridize with Frankia 16S rRNA targeted oligonucleotide probes, indicating that they did belong to the genus Frankia. Furthermore, by nifHDK probe hybridizations, the homologous fragments of nifHDK genes were detected among the bacteria, and they were located in various sizes of restriction fragments of total DNA, showing diverse patterns of restriction fragment length polymorphisms of nifHDK gene (nifHDK_RFLPs). The PCR_based amplification and cloning of nifH gene throw light on the molecular phylogeny of Coriaria_infective Frankia.展开更多
From the experiment of extraction of leaves of Coriaria sinica Maxim leaves and the effective inhibitory against 19 kinds of the fungus and bacteria,the result showed the alcoholoes compounds was more efficious than a...From the experiment of extraction of leaves of Coriaria sinica Maxim leaves and the effective inhibitory against 19 kinds of the fungus and bacteria,the result showed the alcoholoes compounds was more efficious than aliphatic compounds to every microoganism.For Ralstonia solanacearum as an example,Studying on the way of the suppression medicament working,it was found that the compoundscan controled Ralstonia solanacearum during its logarithmic phaseand stable trophophase.Through the photos of electron microscope,it was found that the compound can make the bacteria’chromatin pycnosis and appeard many(vacuoles,)destruct the formation of the cell wall then break the bacteria.展开更多
建立了超高效液相色谱-三重四极杆质谱联用技术测定血浆和尿液中马桑中毒标志物马桑亭和马桑宁的方法。血浆和尿液样品经固相支持液液萃取法提取净化后,溶于15%(v/v)甲醇水溶液中,以Cortecs C_(18)色谱柱(100 mm×2.1 mm, 1.6μm)...建立了超高效液相色谱-三重四极杆质谱联用技术测定血浆和尿液中马桑中毒标志物马桑亭和马桑宁的方法。血浆和尿液样品经固相支持液液萃取法提取净化后,溶于15%(v/v)甲醇水溶液中,以Cortecs C_(18)色谱柱(100 mm×2.1 mm, 1.6μm)作为分析柱进行分离,电喷雾负离子多反应监测(MRM)模式下检测,以氟苯尼考作为内标物,基质工作曲线内标法定量。血浆和尿液中马桑亭和马桑宁的平均加标回收率为86.2%～110%,相对标准偏差为5.1%～14.6%(n=6),血浆中马桑亭和马桑宁的检出限(S/N=3)分别为0.01μg/L和0.1μg/L,尿液中马桑亭和马桑宁的检出限分别为0.03μg/L和0.3μg/L。本法简单、灵敏、准确,可用于血浆和尿液中马桑亭和马桑宁的中毒检测。展开更多
基金Financial support from the National Natural Science Foundation of China(No.21132009)the National Science and Technology Project of China(No.2012ZX09301002-002)
文摘One new sesquiterpene lactone, corialactone E （1）, one new neolignan, coriarianeolignan A （2）, together with three known apocarotenoids （3-5） and one known neolignan （6） have been isolated from a CHCI3 extract of the roots of Coriaria nepalensis. The structures including absolute configurations of 1-6 were elucidated through extensive NMR, HR-ESIMS, and CD data analysis. Structurally, compound I possessed novel variations in the structure, including the newly formed ether ring of C-3/O/C-9 and the lactone ring connecting C-13 and C-5. Compound 5 showed cytotoxic activity against SKOV3 （human ovarian cancer） cells with IC50 values of 4.67 μmol/L. In vivo system, compound 3 showed anti-convulsant activity by 34% at the dose of 5 mg/kg.
基金supported by the Major State Basic Research Development Program of China(Nos. 2009CB522303 and 2009CB940900)the foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China(No.P2008-ZZ13)
文摘Two new compounds. corialins A （1） and B （2） were isolated from Coriaria nepulensis Wall. These new compounds were established as 7-hydroxy-3-[2,3-acetonidc-（3-methylhutane）] coumarin （1） and 3-O-β-D-glucopyranosyl-3,4,5-1rihydroxy-1-（3- methyl-2-butenyl）-benzene （2）. on the basis of ID and 2D NMR techniques.
文摘Four strains with typical morphology of actinomycete genus of Frankia were isolated from root nodules of Coriaria nepalensis. They were shown to nodulate the seedlings of host plant and hybridize with Frankia 16S rRNA targeted oligonucleotide probes, indicating that they did belong to the genus Frankia. Furthermore, by nifHDK probe hybridizations, the homologous fragments of nifHDK genes were detected among the bacteria, and they were located in various sizes of restriction fragments of total DNA, showing diverse patterns of restriction fragment length polymorphisms of nifHDK gene (nifHDK_RFLPs). The PCR_based amplification and cloning of nifH gene throw light on the molecular phylogeny of Coriaria_infective Frankia.
文摘From the experiment of extraction of leaves of Coriaria sinica Maxim leaves and the effective inhibitory against 19 kinds of the fungus and bacteria,the result showed the alcoholoes compounds was more efficious than aliphatic compounds to every microoganism.For Ralstonia solanacearum as an example,Studying on the way of the suppression medicament working,it was found that the compoundscan controled Ralstonia solanacearum during its logarithmic phaseand stable trophophase.Through the photos of electron microscope,it was found that the compound can make the bacteria’chromatin pycnosis and appeard many(vacuoles,)destruct the formation of the cell wall then break the bacteria.
文摘建立了超高效液相色谱-三重四极杆质谱联用技术测定血浆和尿液中马桑中毒标志物马桑亭和马桑宁的方法。血浆和尿液样品经固相支持液液萃取法提取净化后,溶于15%(v/v)甲醇水溶液中,以Cortecs C_(18)色谱柱(100 mm×2.1 mm, 1.6μm)作为分析柱进行分离,电喷雾负离子多反应监测(MRM)模式下检测,以氟苯尼考作为内标物,基质工作曲线内标法定量。血浆和尿液中马桑亭和马桑宁的平均加标回收率为86.2%～110%,相对标准偏差为5.1%～14.6%(n=6),血浆中马桑亭和马桑宁的检出限(S/N=3)分别为0.01μg/L和0.1μg/L,尿液中马桑亭和马桑宁的检出限分别为0.03μg/L和0.3μg/L。本法简单、灵敏、准确,可用于血浆和尿液中马桑亭和马桑宁的中毒检测。
