Isolation and Characterization of a Bioactive Polysaccharide from Panax Quinquefolium L.

ＩｓｏｌａｔｉｏｎａｎｄＣｈａｒａｃｔｅｒｉｚａｔｉｏｎｏｆａＢｉｏａｃｔｉｖｅＰｏｌｙｓａｃｃｈａｒｉｄｅｆｒｏｍＰａｎａｘＱｕｉｎｑｕｅｆｏｌｉｕｍＬ．ＭＡＸｉｕ－ｌｉ＊，ＨＡＯＣｈｕｎ－ｙａｎ，ＬＵＳｈｉ－ｘｉａｎｇ，ＳＵＮＹｕｎ－ｘｉｕ，Ｌ...

西洋参多糖5-2的分离、性质和活性研究

目的：研究西洋参多糖（ＰＰＱ）中均一组分ＰＰＱ５２的分离、糖组成、相对分子量及生物活性。方法：ＳｅｐｈａｄｅｘＤＥＡＥＡ５０和ＳｅｐｈａｄｅｘＧ１００分离，气相色谱法测定糖组成，基底辅助激光解吸／电离质谱测定相对分子量。用小鼠脾淋巴细胞进行淋巴细胞转化实验和白介素２促诱生实验。结果：ＰＰＱ５２由Ｄ阿拉伯糖和Ｄ半乳糖以１∶１．１摩尔比组成，含糖醛酸，相对分子量２１０００Ｄ，具有刺激淋巴细胞转化及白介素２促诱生功能。

西洋参多糖PPQI-1～4理化性质的研究

AIM: To study the characteristics of the four bioactive pure polysaccharides of Panax quiquefolium L. (PPQI 1～4). METHODS: The polysaccharides were extracted with hot water and grade precipitated with ethanol. They were further purified with Sephadex DEAE A50 and Sephadex G200 chromatography. The molecular weights of the polysaccharides were determined with matrix assisted laser desorption/ionization mass spectrometry (MALDI MS). The sugar compositions were analyzed with GC. The linkage positions of the component sugars were determined by methylation and GC MS. RESULTS: The four polysaccharides are all uronic acid containing heteropolysaccharides with the component sugars of arabinose, galactose and glucose in different molar ratios. The uronic acid contents are within 30%～60%. Their molecular weights are in the range of 2×10 4～7×10 4 u. CONCLUSION: The four new bioactive polysaccharides are all uronic acid containing heteropolysaccharides.

川芎哚的有机合成研究

目的：探讨有机合成川芎哚的方法。方法：以糠醇、色氨酸为原料（路线１）和以糠醇、色胺为原料（路线２）两种途径，经多步反应合成川芎哚。结果：路线１制备川芎哚的终产率为７％，其结构经ＵＶ，ＩＲ，ＭＳ和ＮＭＲ确证；路线２制备川芎哚的终产率为１２％，其结构经ＭＳ，ＵＶ确证。结论：两种合成路线都适于合成川芎哚，但路线２的产率高于路线１。

基质辅助激光解吸电离飞行时间质谱技术在13价肺炎球菌多糖蛋白结合疫苗生产用菌种质量控制中的作用

目的探讨基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF MS)技术在13价肺炎球菌多糖蛋白结合疫苗生产用菌种质量控制中的作用。方法采用《中国药典》三部(2020版)中规定方法对13种血清型共24株肺炎球菌的经典表型进行鉴定,应用MALDI-TOF MS技术构建蛋白指纹图谱,根据质谱图的相似性进行聚类分析。同时采用MALDI-TOF MS技术检测不同荚膜丰度14型肺炎球菌(Streptococcus pneumoniae serotype 14,FL14)、不同来源FL5菌种及不同批次FL5工作菌种的蛋白指纹图谱的差异。结果13种血清型肺炎球菌均符合相关要求,蛋白指纹图谱存在差异,共分为3个聚类。不同荚膜丰度FL14的蛋白指纹图谱一致;FL5丹麦菌种及北京儿科医院菌种的指纹图谱有差异,且为不同聚类;不同批次FL5工作菌种的蛋白指纹谱图一致,均为同一个聚类。结论MALDI-TOF MS技术可作为经典细菌表型鉴定外的补充方法,丰富肺炎球菌菌种信息,可应用于疫苗用生产菌种的质量控制。