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Tagetes erecta Linn.and its mosquitocidal potency against Culex quinquefasciatus 被引量:11
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作者 Farjana Nikkon M Rowshanul Habib +1 位作者 Zahangir Alam Saud M Rezaul Karim 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2011年第3期186-188,共3页
Objective:To investigate mosquitocidal effects of ethanolic extract of(lowers of Tagetes erecta(T.erecta) and its chloroform and petroleum ether soluble fractions against the larvae of Culex quinquefasciatus(Cx.quinqu... Objective:To investigate mosquitocidal effects of ethanolic extract of(lowers of Tagetes erecta(T.erecta) and its chloroform and petroleum ether soluble fractions against the larvae of Culex quinquefasciatus(Cx.quinquefasciatus).Methods:The fresh flowers of T.erecta were extracted in cold with ethanol(5.0 L) and after concentration,the ethanol extract was fractionated with chloroform and petroleum ether to afford a brownish syrupy suspension of ethanol extract(50.0 g),petroleum ether soluble fraction(18.6 g) and chloroform soluble fraction(23.8 g).The larvicidal effect of ethanol extract and their solvent fractions were determined by the standard procedure of WHO against different instars of Cx.quinquefasciatus.Results:Among the tested samples the chloroform soluble fractions showed the highest toxicity and consequently,the lowest LC_(50) values(14.14 μ g/mL,17.06 μ g/mL.36.88 μ g/mL and 75.48 μ g/mL) for all the instars larvae of Cx.quinquefasciatus.The larvae showed comparative tolerance in the course of increasing age and time.Conclusions:It can be concluded that the flowers of T.erecta are very effective natural larvicide and could be useful against Cx.quinquefasciatus. 展开更多
关键词 tagetes erecta Linn. CULEX quinquefasciatus Say Mosquitocidal Larvae LARVICIDAL effect Toxicity
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Comparative transcriptome analysis of Ts(Resistant genotype)and Ma(Susceptible genotype)marigold(Tagetes erecta L.)leaves in response to Alternaria tagetica 被引量:1
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作者 Xi Cheng Dongliang Chen +2 位作者 Chang Luo Hua Liu Conglin Huang 《Horticultural Plant Journal》 SCIE CAS CSCD 2023年第2期321-334,共14页
Marigold black spot caused by Alternaria tagetica is a major disease that can decrease marigold production by 40%,resulting in serious economic losses.In this study,we identified many genes responsive to A.tagetica in... Marigold black spot caused by Alternaria tagetica is a major disease that can decrease marigold production by 40%,resulting in serious economic losses.In this study,we identified many genes responsive to A.tagetica in the resistant and susceptible marigold genotypes.Analyses of differentially expressed genes,expression trends,and a weighted gene co-expression network revealed a series of hub genes with key roles in different A.tagetica infection stages.Additionally,1216 unigenes encoding transcription factors from eight families were differentially expressed between Ts and Ma.Moreover,R genes fromvarious families(e.g.,N,NL,RLP,and TNL)were differentially expressed in the twomarigold genotypes before and after the inoculation with A.tagetica.Pathway diagrams were used to visualize the leaf transcriptional changes in the two marigold genotypes infected by A.tagetica to clarify the effects of A.tagetica on the expression patterns of genes involved in phosphatidylinositol signaling,plant–pathogen interactions,and plant hormone signal transduction.We identified candidate genes related to disease resistance and generated valuable resources for analyzing the candidate gene functions related to black spot resistance in marigold.The study data may be useful for the molecular marker-assisted screening and breeding of marigold lines with increased disease resistance. 展开更多
关键词 tagetes erecta Co-expression network Gene expression Black spot resistance Transcriptional modules
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Comparative Efficacy of <i>Tagetes erecta</i>and <i>Centella asiatica</i>Extracts on Wound Healing in Albino Rats 被引量:1
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作者 Sandipan Chatterjee Tigari Prakash +2 位作者 Dupadahalli Kotrsha Nadendla Rama Rao Divakar Goli 《Chinese Medicine》 2011年第4期138-142,共5页
The study was undertaken to evaluate the comparative efficacy of hydroalcoholic extracts of leaves of Tagetes erecta (T. erecta) and aerial parts of Centella asiatica (C. asiatica) on Excision, Incision and Dead space... The study was undertaken to evaluate the comparative efficacy of hydroalcoholic extracts of leaves of Tagetes erecta (T. erecta) and aerial parts of Centella asiatica (C. asiatica) on Excision, Incision and Dead space wound models in albino rats. Extract of T. erecta and C. asiatica (P T. erecta extract (250 and 500 mg/kg) showed significantly increased the wound breaking strength in incision wound model and wet and dry granulation tissue weights, breaking strength in a dead space wound model compare to control and C. asiatica treated group (P T. erecta extract showed potent wound healing activity then the reported C. asiatica in different wound parameters. 展开更多
关键词 CENTELLA asiatica tagetes erecta Excision INCISION Dead Space Wound Model
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Chromosome-scale genome assembly of marigold (Tagetes erecta L.):An ornamental plant and feedstock for industrial lutein production 被引量:1
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作者 Haibo Xin Fangfang Ji +7 位作者 Jie Wu Shiya Zhang Caijie Yi Shiwei Zhao Richen Cong Liangjun Zhao Hua Zhang Zhao Zhang 《Horticultural Plant Journal》 SCIE CAS CSCD 2023年第6期1119-1130,共12页
Marigold(Tagetes erecta L., African marigold) is a widely grown ornamental plant and a main source of the carotenoid lutein for the industrial production of pharmaceuticals, food coloring, and feed additives. To gain ... Marigold(Tagetes erecta L., African marigold) is a widely grown ornamental plant and a main source of the carotenoid lutein for the industrial production of pharmaceuticals, food coloring, and feed additives. To gain a deeper understanding of the genetic mechanism of lutein in marigold, a chromosome-scale assembly of the marigold(T. erecta V-01) genome was completed based on Illumina, PacBio, and Hi-C reads. The707.21-Mb assembled genome consisted of 35 834 annotated protein-coding genes, with 97.7% genomic integrity. We anchored 87.8% of the contigs(covering 621.20 Mb) to 12 pseudochromosomes, bringing the scaffold N50 length to 54.15 Mb. Phylogenetic analysis showed that marigold was closely related to the Asteraceae species bitter vine(Mikania micrantha) and sunflower(Helianthus annuus), all three of which originated in the Americas. Marigold diverged from the sunflower clade 23.57 million years ago(MYA) and from M. micrantha 19.59 MYA.Marigold has undergone three whole-genome duplication events, as well as a recent whole-genome duplication event(WGD-2) common to H.annuus and M. micrantha. Marigold gene families were significantly less expanded than those of M. micrantha or H. annuus, and the marigold genome contained significantly fewer interspersed repeats, which might account for its smaller genome. In addition, a range of candidate genes involved in the lutein biosynthetic pathway were identified. The high-quality reference genome obtained in this study provided a valuable genomic resource for studying the evolution of the Asteraceae family and for improving marigold breeding strategies. 展开更多
关键词 T.erecta L. Genome Gene family CAROTENOID LUTEIN
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Toxicity of Tagetes erecta Essential Oil Based Combinatorial Formulations on Various Metabolic Enzymes in Indian White Termite Odontotermes obesus
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作者 Susheel Kumar Ravi Kant Upadhyay 《Advances in Enzyme Research》 2023年第2期58-81,共24页
Plant essential oils and their constituents have proven to be very effective against insects, especially termites. They are the best alternative to synthetic pesticides that are harmless to the environment and human h... Plant essential oils and their constituents have proven to be very effective against insects, especially termites. They are the best alternative to synthetic pesticides that are harmless to the environment and human health. In the present study, different enzymes, namely alkaline phosphatase, acid phosphatase, glutamate-oxaloacetate transaminase, glutamate-pyruvate transaminase and acetylcholinesterase, were evaluated in Indian termite Odontotermes obesus in a combination preparation based on Tagetes erecta essential oil. For the study of anti-termite effects against worker termites were treated topically with 40% and 80% of the 24-hour LD50 values of various combination formulations. Subsequent bioassays at 40% and 80% of LD50 of combinatorial preparations S-AST-A, S-AST-B, and S-AST-C resulted in a significant (p > 0.05) decrease in glutamate-oxaloacetate transaminase. After 16 hours of treatment, they were 87.92%, 80.17%, 89.30%, 79.17%, 81.92% and 73.17% respectively. In vivo exposures of 40% and 80% of the LD50 of the combination formulation caused a highly significant (p > 0.05) reduction (p > 0.05) of all test enzymes tested compared to controls. The effects of different oil formulations exhibited time- and dose-dependent responses, resulting in physiological changes in the concentrations of various enzymes. The combined mixture of Tagetes erecta essential oils has significantly better anti-termite ability compared to inorganic insecticides. Findings from this study will help support termite control in fields, gardens and homes in a sustainable way, without the downsides of insecticide resistance and pollution. These could potentially be used to produce commercial formulations for use against pests. 展开更多
关键词 tagetes erecta Essential Oils Odontotermes obesus ENZYMES INHIBITION Termiticidal Action
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Termiticidal and Biochemical Activity of Combinatorial Essential Oil Ingredients of Tagetes erecta on Indian White Termite Odontotermes obesus
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作者 Susheel Kumar Ravi Kant Upadhyay 《Journal of Biosciences and Medicines》 2023年第4期114-136,共23页
In present investigation, various bio-molecular parameters i.e. glycogen, protein, amino acid, DNA, RNA and lipid, were determined for evaluation of anti-termite efficacy of Tagetes erecta essential oil based combinat... In present investigation, various bio-molecular parameters i.e. glycogen, protein, amino acid, DNA, RNA and lipid, were determined for evaluation of anti-termite efficacy of Tagetes erecta essential oil based combinatorial formulations against Indian white termite Odontotermes obesus. For this purpose, 40% and 80% of 24 hrs of LD50 values of various formulations were provided for treatment of termite workers. Observations were taken at 4 hrs time interval up to 16 hrs to know inhibitory activity of these combinatorial mixtures on termite species Odontotermes obesus. Significant (p > 0.05) alterations were observed in the level above mentioned bio-molecules when termites were treated with different combinatorial essential oils ingredients of Citrus maxima. Combinatorial mixtures of Tagetes erecta essential oils have shown synergistic activity against termites. This study will provide an overall sustainable way to termite control in crop field, gardens and houses. It also suggests use of essential oils as better alternative of synthetic termiticides as these are safer for environmental and human health. 展开更多
关键词 tagetes erecta Odontotermes obesus Plant Essential Oils Termiticides Bio-Molecules INHIBITION Sustainable
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Application of Statistic Analysis on the Selection of Quantitative Characteristics Listed in the DUS Test Guideline of Tagetes L. 被引量:1
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作者 刘艳芳 张建华 +6 位作者 杨晓洪 王江民 李彦刚 陈海荣 李向东 张惠 管俊娇 《Agricultural Science & Technology》 CAS 2012年第9期2021-2023,2033,共4页
[Objective] This paper researched how to use specific approach to select quantitative characteristics which would be listed in the DUS Test Guideline of Tagetes L. [Method] With the aid of statistic analysis, the unif... [Objective] This paper researched how to use specific approach to select quantitative characteristics which would be listed in the DUS Test Guideline of Tagetes L. [Method] With the aid of statistic analysis, the uniformity, stability and correlation of 14 pre-selected characteristics were analyzed. [Result] The expression of peduncle length of terminal flower had low uniformity within a variety; the expres- sion of main stem thickness was not stable in continuous growing cycles; there were four pairs of quantitative characteristics which are (very) significantly correlated, namely, leaf length and leaf width, diameter of flower head and length of outer ligu- late floret, number of ligulate floret whorls and number of ligulate floret, leaf width and width of terminal leaflet of pinnate leaves. Based on the requirements of char- acteristic selection and the results of statistic analysis, five characteristics including peduncle length of terminal flower, thickness of the main stem, leaf width, length of outer ligulate floret and number of ligulate floret were deleted. [Conclusion] For the first time, this paper introduced the application of statistic analysis on the selection of quantitative characteristics. 展开更多
关键词 DUS Test Guideline of tagetes L. Quantitative characteristic Statisticanalysis
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Selection of Example Varieties Used in the DUS Test Guideline of Tagetes L. 被引量:1
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作者 刘艳芳 张建华 +6 位作者 王烨 陈海荣 徐云 杨晓洪 张惠 管俊娇 王江民 《Agricultural Science & Technology》 CAS 2012年第10期2110-2111,2116,共3页
[Objective] Taking the characteristic of flower diameter of Tagetes L.as an example,this study aimed to select example varieties used in the DUS Test Guideline of Tagetes L.[Method] Two continuous years of measurement... [Objective] Taking the characteristic of flower diameter of Tagetes L.as an example,this study aimed to select example varieties used in the DUS Test Guideline of Tagetes L.[Method] Two continuous years of measurements of flower diameter of 25 varieties were collected and then analyzed by using the box plot to illustrate the uniformity and stability of flower diameter of each variety.[Result] According to the information of variability,distribution symmetry of measurements and outliers of flower diameter of varieties provided by box plots,variety 16,2 and 4 were selected as the example varieties for the three expression states with respective flower diameter of 3.0-4.4,6.0-7.4 and 9.0-10.4 cm.[Conclusion] The box plot is an efficient method for the general analysis of varieties,which provides information covering the actual and possible expression range,median and outliers of measurements of flower diameter of each variety.It also provides references for selecting example varieties for other quantitative characteristics and evaluating the quality of varieties. 展开更多
关键词 DUS Test Guideline of tagetes L. Quantitative characteristic Example variety The box plot
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Analysis on the Functions and Properties of the Preliminarily Determined Characteristics Listed in DUS Test Guideline of Tagetes L.
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作者 刘艳芳 张建华 +5 位作者 杨晓洪 张惠 陈海荣 徐云 管俊娇 王江民 《Agricultural Science & Technology》 CAS 2012年第8期1789-1792,共4页
[Objective] This study aimed to investigate the functions and properties of the preliminarily determined characteristics listed in DUS test guideline of Tagetes L., and explore the representativeness and comprehensive... [Objective] This study aimed to investigate the functions and properties of the preliminarily determined characteristics listed in DUS test guideline of Tagetes L., and explore the representativeness and comprehensiveness of this group of characteristics in DUS test. [Method] Based on the functions and properties of the characteristics, the described plant part (s), observation stage, expression pattern and observation method of each characteristic were analyzed to illustrate the representativeness and comprehensiveness of the combination of this group of characteristics in above functions and properties. [Result] As for described plant part(s), there are 5 characteristics describing plant as a whole, 3 characteristics describing stem, 6 characteristics describing leaf, 23 characteristics describing flower and 1 characteristic describing physiological feature. As for observation stage, there are 1 characteristic needing to be observed in the stage of seedling, 1 characteristic in the stage of beginning of flowering and other 36 characteristics in the stage of fully flowering. As for the expression pattern, there are 10 qualitative characteristics, 9 pseudo-qualitative characteristics and 19 quantitative characteristics. As for the observation method, there are 30 characteristics using VG as the observation method, and 8 characteristics using MS. [Conclusion] In view of the variation and morphological properties of marigold, this group of characteristics are representative and comprehensive, and ensure the accuracy and easiness of DUS test of Tagetes L., thereby achieving the reasonable combination of characteristics in described plant parts, observation stages, expression patterns and observation methods. 展开更多
关键词 DUS test guideline of tagetes L. CHARACTERISTIC Function PROPERTY
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Anti-oxidative and anti-inflammatory effects of Tagetes minuta essential oil in activated macrophages 被引量:2
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作者 Parastoo Karimian Gholamreza Kavoosi Zahra Amirghofran 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2014年第3期219-227,共9页
Objective:To investigate antioxidant and anti-inflammatory effects of Tagetes minuta(T.minuta)essential oil.Methods:In the present study T.minuta essential oil was obtained from leaves of T.minuta via hydro-distillati... Objective:To investigate antioxidant and anti-inflammatory effects of Tagetes minuta(T.minuta)essential oil.Methods:In the present study T.minuta essential oil was obtained from leaves of T.minuta via hydro-distillation and then was analyzed by gas chromatography-mass spectrometry.The antioxidant capacity of T.minuta essential oil was examined by measuring reactive oxygen,reactive nitrogen species and hydrogen peroxide scavenging.The anti-inflammatory activity of T.minuta essential oil was determined through measuring NADH oxidase,inducible nitric oxide synthase and TNF-αmRNA expression in lipopolysacharide-stimulated murine macrophages using realtime PCR.Results:Gas chromatography-mass spectrometry analysis indicated that the main components in the T.minuta essential oil were dihydrotagetone(33.86%),E-ocimene(19.92%).tagetone(16.15%),cis-β-ocimene(7.94%),Z-ocimene(5.27%).limonene(3.1%)and epoxyocimene(2.03%).The T.minuta essential oil had the ability to scavenge all reactive oxygen/reactive nitrogen species radicals with IC_(50)12-15μg/mL,which indicated a potent radical scavenging activity.In addition,T.minuta essential oil significantly reduced NADH oxidase,inducible nitric oxide synthaseand TNF-αmRNA expression in the cells at concentrations of 50μg/mL,indicating a capacity of this product to potentially modulate/diminish immune responses.Conclusions:T.minuta essential oil has radical scavenging and anti-inflammatory activities and could potentially be used as a safe effective source of natural anti-oxidants in therapy against oxidative damage and stress associated with some inflammatory conditions. 展开更多
关键词 tagetes minuta ESSENTIAL OIL MACROPHAGES ANTI-INFLAMMATORY ANTIOXIDANT
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Activity of Tagetes minuta Linnaeus(Asteraceae)essential oil against L3 Anisakis larvae type 1 被引量:1
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作者 Filippo Giarratana Daniele Muscolino +5 位作者 Graziella Ziino Alessandro Giuffrida Stefania Maria Marotta Vittorio Lo Presti Vincenzo Chiofalo Antonio Panebianco 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2017年第5期440-444,共5页
Objective:To evaluate in vitro effects of Tagetes minuta L. essential oil(TEO) on L3 Anisakis larvae type 1. Methods:In order to evaluate the potential use of Tagetes minuta essential oil against L3 Anisakis larvae th... Objective:To evaluate in vitro effects of Tagetes minuta L. essential oil(TEO) on L3 Anisakis larvae type 1. Methods:In order to evaluate the potential use of Tagetes minuta essential oil against L3 Anisakis larvae three different media were tested:1) a saline solution(SS); 2) an industrial marinating solution; 3) sunflower seeds oil(SO). For each media and concentrations of TEO(0.1%,0.5%,1.0% and 5.0% v/v),20 parasites were introduced into plastic Petri dishes(diameter 90 mm) and maintained at room temperature. As controls,larvae were maintained without TEO under identical experimental conditions in SS,MS and SO. A total of 900 larvae were tested. The normalized mean viability,LT100,LT50 and the percentage of inactivation at 24 hours were calculated. Results:In vitro tests revealed a complete inactivation of parasites in saline solution after 2 hours with 5% and 1% of TEO. In marinating solution,a complete inactivation of parasites was observed after 4 hours at all concentrations used. A slower activity for all TEO concentration was reported in SO. Conclusions:The results obtained,showing a strong activity against Anisakis larvae,confirm TEO as a larvicidal agent in the treatment of human anisakidosis and in the industrial marinating process. 展开更多
关键词 ANISAKIS Essential oil tagetes minuta Biocidal activity Parasites
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Study on Application Technology for Tissue Culture and Propagation of Tagetes patula L.
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作者 Rundong FENG Chunhui LI Xiaojie TANG 《Agricultural Biotechnology》 CAS 2012年第2期12-13,16,共3页
[ Objective] This study aimed to investigate the tissue culture and propagation technology in Tagetes patu/a L. [ Method] By using tissue culture tech- nology, different mass fractions of 6-BA and NAA were added to MS... [ Objective] This study aimed to investigate the tissue culture and propagation technology in Tagetes patu/a L. [ Method] By using tissue culture tech- nology, different mass fractions of 6-BA and NAA were added to MS medium to compare the effect of different culture medium on the rapid propagation of T. patu/a L. [Result] Shoot tips or stem segments of T. patu/a L. were used as explants for tissue culture with an appropriate sterilization time of 8 min; differentiation effect of shoot tips was better than that of stem segments; callus generation rate was high with the high content of growth regulators; MS medium containing O. 1 mg/L NAA and 1.5 rag/L 6-BA was used for subculture proliferation with a subculture period of 4 weeks; rooting rate of plantlet was the maximum (97%) in 1/2MS medium containing 0.2 mg/L NAA, and the root system was relatively developed. [ Conclusion] This study provided technical support for the industrialized seedling breeding of T. patula L. 展开更多
关键词 tagetes patu/a L. Tissue culture Rapid propagation
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Two agronomical aspects of Tagetes terniflora HBK for essential oil production
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作者 Miguel ángel Serrato Cruz Francisco Díaz Cedillo +1 位作者 Daniel Hernández Medina Arturo Curiel Rodríguez 《Journal of Agricultural Chemistry and Environment》 2014年第1期9-13,共5页
The genus Tagetes is a possible source of essential oils for the biorational control of pests and diseases in Mexico. The aim of the present study was to assess the distance between plants (PD;15 × 80, 30 × ... The genus Tagetes is a possible source of essential oils for the biorational control of pests and diseases in Mexico. The aim of the present study was to assess the distance between plants (PD;15 × 80, 30 × 80 and 60 × 80 cm) and urea fertilization (0, 60 and 120 kg·he-1 N) for biomass production and essential oil in Tagetes terniflora HBK. Oil was obtained by the aerial part hydro-distillation and its chemical composition was analyzed by gas chromatography coupled to mass spectrometry. Factors N, PD and their interaction did not change plant height or the number of branches per plant;however, PD factor modified fresh tissue (FW) and dry tissue (DW) weights and the amount of oil per plant (p ≤ 0.05) which represent a good biomass production (30 to 72.5 ton·he-1 FW, 11 to 27 ton·he-1 DW) and oil producing (168 to 383 L·he-1 ) potential in the field. Urea did not have effect on both biomass and oil response per plant but interaction of 15 x 80 DP and 120 N could favor higher biomass and oil production potential (78 ton FW, 28 ton·he-1 DW and 608 L·he-1 , respectively). Essential oil yield varied from 0.3 to 2.1% according to the management conditions. A total of 11 major compounds were identified in essential oil, the relative quantity was constant in different agronomic management factors: E- tagetone (22%), cistagetenone (20.4%), transtagetenone (20.4%), dihydrotagetone (13.4%) and cis-β-ocimene (10.3%), trans-β-ocimene (5.0%), propenyl anisole (4.3%), sphatulenole (1.1%), allyl anisole (0.7%), Z-tagetone (0.5%) and limonene (0.5%). 展开更多
关键词 tagetes terniflora BIOMASS ESSENTIAL Oil Chemical Composition DISTANCE between PLANTS NITROGEN
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ERECTA基因研究进展 被引量:6
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作者 胡鑫 徐全乐 《西北植物学报》 CAS CSCD 北大核心 2010年第12期2564-2569,共6页
ERECTA是从拟南芥中分离到的一个类受体激酶基因。ERECTA基因对植物的叶、花和气孔发育都有直接的影响,并在植物抗病、蒸腾作用和元素积累等生理过程中起着重要的作用。该文对国内外有关ERECTA基因在以上几个方面的研究进展进行综述,为... ERECTA是从拟南芥中分离到的一个类受体激酶基因。ERECTA基因对植物的叶、花和气孔发育都有直接的影响,并在植物抗病、蒸腾作用和元素积累等生理过程中起着重要的作用。该文对国内外有关ERECTA基因在以上几个方面的研究进展进行综述,为进一步研究ERECTA基因在植物发育和生理代谢调控网络中的地位及在植物遗传育种上的应用提供参考。 展开更多
关键词 erecta基因 植物发育 植物抗病 蒸腾效率 元素积累
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3种黑杨无性系水分利用效率差异性分析及相关ERECTA基因的克隆与表达 被引量:4
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作者 郭鹏 夏新莉 尹伟伦 《生态学报》 CAS CSCD 北大核心 2011年第11期3239-3245,共7页
提高植物水分利用效率(WUE)是未来解决我国甚至世界干旱缺水的最重要手段之一。因此,培育和筛选高WUE的品系和品种意义重大。通过对3种在充分浇水处理下不同北美黑杨无性系WUE、叶片δ13C的差异分析,发现WUE在黑杨无性系之间也存在差异... 提高植物水分利用效率(WUE)是未来解决我国甚至世界干旱缺水的最重要手段之一。因此,培育和筛选高WUE的品系和品种意义重大。通过对3种在充分浇水处理下不同北美黑杨无性系WUE、叶片δ13C的差异分析,发现WUE在黑杨无性系之间也存在差异。ERECTA基因可以通过改变气孔密度来提高WUE。在欧美杨中克隆到了该基因(PdERECTA)。该基因序列长2844bp,编码948个氨基酸,并且具有保守的类受体蛋白激酶家族的膜外富含亮氨酸和胞内激酶区域。荧光定量表达分析证明该基因在ABA、盐、冷等胁迫处理下都被诱导表达。组织特异表达分析说明PdERECTA基因在顶端叶表达量最高,半成熟叶次之,成熟叶衰老叶和根中则无表达。 展开更多
关键词 erecta基因 北美黑杨 叶片δ13C 水分利用效率(WUE)
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葡萄ERECTA基因过表达提高拟南芥生长量和耐热性
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作者 刘敏 房玉林 《生物技术通报》 CAS CSCD 北大核心 2020年第6期46-53,共8页
葡萄ERECTA基因受高温诱导表达,且在葡萄的幼叶和花中表达量较高。为了研究葡萄ERECTA基因在植物生长发育和高温响应中的作用,将该基因在拟南芥中过表达,观察其表型变化和对高温的耐受性。结果表明,葡萄ERECTA基因过表达可以显著增加拟... 葡萄ERECTA基因受高温诱导表达,且在葡萄的幼叶和花中表达量较高。为了研究葡萄ERECTA基因在植物生长发育和高温响应中的作用,将该基因在拟南芥中过表达,观察其表型变化和对高温的耐受性。结果表明,葡萄ERECTA基因过表达可以显著增加拟南芥叶片的长度、宽度和数量,花梗变细长,角果长度和株高显著增加。将拟南芥种子在高温处理后常温培养2 d,大部分Landsberg erecta(Ler)幼苗发育不正常,而过表达型ER-OE幼苗均未受到高温胁迫的影响。拟南芥植株在40℃高温处理4 d,ER-OE植株受胁迫程度较轻,叶片电导率的顺序为Ler>Col>ER-OE;在22℃恢复5 d后,Col、Ler和ER-OE的存活率分别为33%、12%和83%。ERECTA基因既可以提高植株的耐热性,又可以增加生长量,为培育葡萄抗性新品种提供了新的基因资源,在农业生产上有很好的应用前景。 展开更多
关键词 葡萄 erecta 生长发育 高温 耐热性
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基于In-fusion技术的ERECTA基因植物表达载体的构建 被引量:5
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作者 陈菲帆 王琪琦 +3 位作者 钟辉丽 付冰冰 任丹莉 李玉红 《北方园艺》 CAS 北大核心 2015年第18期110-115,共6页
以黄瓜品种‘长春密刺’(CCMC)为试材,利用高保真酶Iproof及不依赖于酶切的In-fusion技术,构建基于本底启动子驱动的黄瓜ERECTA基因的植物表达载体,以探讨ERECTA基因在黄瓜中的功能,以期为黄瓜抗性性状的遗传改良提供技术支持。结果表明... 以黄瓜品种‘长春密刺’(CCMC)为试材,利用高保真酶Iproof及不依赖于酶切的In-fusion技术,构建基于本底启动子驱动的黄瓜ERECTA基因的植物表达载体,以探讨ERECTA基因在黄瓜中的功能,以期为黄瓜抗性性状的遗传改良提供技术支持。结果表明:从CCMC的基因组DNA中克隆的2段gERECTA(DNA序列)长度分别为8 940bp和9 812bp,并分别命名为CsgERECTA-Flag和CsgERECTA-Poly。经过质粒PCR、梯度片段PCR和测序结果的鉴定表明,pCAMBIA3301-gERECTA的2个植物表达载体都已成功构建并转入根癌农杆菌EHA105中。 展开更多
关键词 黄瓜 erecta 长片段扩增 In-fusion 表达载体
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拟南芥ERECTA基因的克隆及其对番茄转化 被引量:4
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作者 朱锦程 沈海涛 祝建波 《生物技术通报》 CAS CSCD 北大核心 2010年第8期102-105,共4页
全球每年因干旱而造成的作物减产和农业产量损失不可计量,因此从分子生物学上探索植物潜在的耐旱机理对于提高作物抗旱能力具有非凡意义。拟南芥(Ler型)ERECTA基因编码的蛋白存在两个不同的跨膜受体蛋白激酶。通过RT-PCR的方法从拟南芥... 全球每年因干旱而造成的作物减产和农业产量损失不可计量,因此从分子生物学上探索植物潜在的耐旱机理对于提高作物抗旱能力具有非凡意义。拟南芥(Ler型)ERECTA基因编码的蛋白存在两个不同的跨膜受体蛋白激酶。通过RT-PCR的方法从拟南芥基因中克隆出ERECTA基因,构建真核表达载体pBin438-ERECTA,转化到番茄中,为后续研究其对植物水分的利用效率奠定基础。 展开更多
关键词 拟南芥 番茄 erecta基因 转录因子
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ERECTA基因家族调控植物形态发育机制研究进展 被引量:1
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作者 赵雪雅 张坤鹏 +2 位作者 张汇东 毕梦希 齐明芳 《沈阳农业大学学报》 CAS CSCD 北大核心 2023年第2期231-238,共8页
ERECTA(ER)是一种富含亮氨酸重复片段(LRR)的类受体激酶(RLKs)基因,RLKs可以参与多种信号通路并在植物发育早期细胞间信号传导中具有关键作用。植物中有少数核心信号通路参与调节植物生长发育中的不同方面,ER家族(ERfs)信号通路是这些... ERECTA(ER)是一种富含亮氨酸重复片段(LRR)的类受体激酶(RLKs)基因,RLKs可以参与多种信号通路并在植物发育早期细胞间信号传导中具有关键作用。植物中有少数核心信号通路参与调节植物生长发育中的不同方面,ER家族(ERfs)信号通路是这些核心信号之一,在植物的形态发生、花序形成和发育、生物胁迫和非生物胁迫的应答过程中都具有重要作用。尽管ERfs对于植物生长的正常发育是不可或缺的,但由于其功能众多,调控机制十分复杂,各个信号通路发生的组织部位与时间都不相同,使得研究ER信号网络变得困难。ER处于各个信号通路较为上游的位置,因此明确ER调控机制对研究植物生长发育和形态建成至关重要。本研究综述了ER蛋白的结构特点和在植物地上部分器官形态发育方面的功能,主要包括在气孔发育、花序结构发育、茎的伸长以及调节茎尖大小等方面的作用,阐述了不同调控过程中的上游配体以及下游通路,为深入研究ER功能和作用机制提供参考。 展开更多
关键词 erecta EPF/EPFL 气孔发育 花序结构 矮化
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拟南芥ERECTA基因的研究进展 被引量:1
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作者 周慧 李晓屿 +2 位作者 Ralf Müller-Xing 宋兴舜 邢倩 《中国农学通报》 2018年第15期93-99,共7页
ERECTA是从拟南芥La-0(Landsberg)中分离出来的一个类受体激酶,它可以与多种基因相互协作控制植物器官的形态建成。其拟南芥突变体Ler(Landsberg erecta)被广泛应用于分子遗传学研究。目前已有文章报道该基因在花序结构,叶片形态,气孔发... ERECTA是从拟南芥La-0(Landsberg)中分离出来的一个类受体激酶,它可以与多种基因相互协作控制植物器官的形态建成。其拟南芥突变体Ler(Landsberg erecta)被广泛应用于分子遗传学研究。目前已有文章报道该基因在花序结构,叶片形态,气孔发育,抗病性等方面具有重要的作用。随着分子生物学与遗传学的发展,ERECTA基因新的功能被逐渐发现。本文综述了ERECTA基因在拟南芥的分生组织,叶,花以及生物与非生物胁迫等方面的功能。 展开更多
关键词 erecta 拟南芥 形态建成 胁迫应答 分生组织
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