Mitochondrial targeting sequence of magnetoreceptor MagR:More than just targeting

Iron-sulfur clusters(ISC)are essential cofactors for proteins involved in various biological processes,such as electron transport,biosynthetic reactions,DNA repair,and gene expression regulation.ISC assembly protein IscA1(or MagR)is found within the mitochondria of most eukaryotes.Magnetoreceptor(MagR)is a highly conserved A-type iron and iron-sulfur cluster-binding protein,characterized by two distinct types of iron-sulfur clusters,[2Fe-2S]and[3Fe-4S],each conferring unique magnetic properties.MagR forms a rod-like polymer structure in complex with photoreceptive cryptochrome(Cry)and serves as a putative magnetoreceptor for retrieving geomagnetic information in animal navigation.Although the N-terminal sequences of MagR vary among species,their specific function remains unknown.In the present study,we found that the N-terminal sequences of pigeon MagR,previously thought to serve as a mitochondrial targeting signal(MTS),were not cleaved following mitochondrial entry but instead modulated the efficiency with which iron-sulfur clusters and irons are bound.Moreover,the N-terminal region of MagR was required for the formation of a stable MagR/Cry complex.Thus,the N-terminal sequences in pigeon MagR fulfil more important functional roles than just mitochondrial targeting.These results further extend our understanding of the function of MagR and provide new insights into the origin of magnetoreception from an evolutionary perspective.

The Application of Nicotiana benthamiana as a Transient Expression Host to Clone the Coding Sequences of Plant Genes

Coding sequences (CDS) are commonly used for transient gene expression, in yeast two-hybrid screening, to verify protein interactions and in prokaryotic gene expression studies. CDS are most commonly obtained using complementary DNA (cDNA) derived from messenger RNA (mRNA) extracted from plant tissues and generated by reverse transcription. However, some CDS are difficult to acquire through this process as they are expressed at extremely low levels or have specific spatial and/or temporal expression patterns in vivo. These challenges require the development of alternative CDS cloning technologies. In this study, we found that the genomic intron-containing gene coding sequences (gDNA) from Arabidopsis thaliana, Oryza sativa, Brassica napus, and Glycine max can be correctly transcribed and spliced into mRNA in Nicotiana benthamiana. In contrast, gDNAs from Triticum aestivum and Sorghum bicolor did not function correctly. In transient expression experiments, the target DNA sequence is driven by a constitutive promoter. Theoretically, a sufficient amount of mRNA can be extracted from the N. benthamiana leaves, making it conducive to the cloning of CDS target genes. Our data demonstrate that N. benthamiana can be used as an effective host for the cloning CDS of plant genes.

Pig pangenome graph reveals functional features of non‑reference sequences

Background The reliance on a solitary linear reference genome has imposed a significant constraint on our compre-hensive understanding of genetic variation in animals.This constraint is particularly pronounced for non-reference sequences(NRSs),which have not been extensively studied.Results In this study,we constructed a pig pangenome graph using 21 pig assemblies and identified 23,831 NRSs with a total length of 105 Mb.Our findings revealed that NRSs were more prevalent in breeds exhibiting greater genetic divergence from the reference genome.Furthermore,we observed that NRSs were rarely found within coding sequences,while NRS insertions were enriched in immune-related Gene Ontology terms.Notably,our investigation also unveiled a close association between novel genes and the immune capacity of pigs.We observed substantial differences in terms of frequencies of NRSs between Eastern and Western pigs,and the heat-resistant pigs exhibited a substantial number of NRS insertions in an 11.6 Mb interval on chromosome X.Additionally,we discovered a 665 bp insertion in the fourth intron of the TNFRSF19 gene that may be associated with the ability of heat tolerance in South-ern Chinese pigs.Conclusions Our findings demonstrate the potential of a graph genome approach to reveal important functional features of NRSs in pig populations.

Phylogenetic, phylogeographic and divergence time analysis of Anopheles subpictus species complex using ITS2 and COI sequences

Objective:To address the phylogenetic and phylogeographic relationship between different lineages of Anopheles(An.)subpictus species complex in most parts of the Asian continent by maximum utilization of Internal Transcriber Spacer 2(ITS2)and cytochrome C oxidase I(COI)sequences deposited at the GenBank.Methods:Seventy-five ITS2,210 COI and 26 concatenated sequences available in the NCBI database were used.Phylogenetic analysis was performed using Bayesian likelihood trees,whereas median-joining haplotype networks and time-scale divergence trees were generated for phylogeographic analysis.Genetic diversity indices and genetic differentiation were also calculated.Results:Two genetically divergent molecular forms of An.subpictus species complex corresponding to sibling species A and B are established.Species A evolved around 37-82 million years ago in Sri Lanka,India,and the Netherlands,and species B evolved around 22-79 million years ago in Sri Lanka,India,and Myanmar.Vietnam,Thailand,and Cambodia have two molecular forms:one is phylogenetically similar to species B.Other forms differ from species A and B and evolved recently in the above mentioned countries,Indonesia and the Philippines.Genetic subdivision among Sri Lanka,India,and the Netherlands is almost absent.A substantial genetic differentiation was obtained for some populations due to isolation by large geographical distances.Genetic diversity indices reveal the presence of a long-established stable mosquito population,at mutation-drift equilibrium,regardless of population fluctuations.Conclusions:An.subpictus species complex consists of more than two genetically divergent molecular forms.Species A is highly divergent from the rest.Sri Lanka and India contain only species A and B.

On power series statistical convergence and new uniform integrability of double sequences

In the present paper,we mostly focus on P_(p)^(2)-statistical convergence.We will look into the uniform integrability via the power series method and its characterizations for double sequences.Also,the notions of P_(p)^(2)-statistically Cauchy sequence,P_(p)^(2)-statistical boundedness and core for double sequences will be described in addition to these findings.

The Upper Jurassic sediments,Marib-Shabwa Basin,Yemen:Lithofacies aspects and sequence stratigraphic analysis

The present study is devoted to understanding the evolution of the Upper Jurassic Sab'atayn Formation in the Marib-Shabwa Basin,Yemen,through a sequence stratigraphic analysis based on integrating datasets of sedimentology,seismic sections,and well logs.The Sab'atayn Formation(Tithonian age)is represented by a series of clastic and evaporites that were deposited under fluvio-deltaic to prodeltaic settings.It is divided into four members including Yah(at the base),upwards to Seen,Alif,and Safir at the top.Two third-order depositional sequences were determined for the Tithonian succession which were separated by three sequence boundaries.These sequences were classified into their systems tracts signifying several sedimentation patterns of progradational,aggradational,and retrogradational parasequence sets.The first depositional sequence corresponds to the early-middle Tithonian Yah and Seen units that can be classified into lowstand,transgressive,and highstand systems tracts.The second sequence comprises the late Tithonian Alif unit that can be subdivided into transgressive and highstand systems tracts.The sandy deposits of the Alif Member(highstand deposits)represent the most productive hydrocarbon reservoir in the basin.The Upper Jurassic sediments in the study area were resulted from a combination of eustatic and tectonic effects.

Landscape of Sequence Variations in Homologous Copies of FAD2 and FAD3 in Rapeseed(Brassica napus L.)Germplasm with High/Low Linolenic Acid Trait

Genetic manipulation(either restraint or enhancement)of the biosynthesis pathway ofα-linolenic acid(ALA)in seed oil is an important goal in Brassica napus breeding.B.napus is a tetraploid plant whose genome often har-bors four and six homologous copies,respectively,of the two fatty acid desaturases FAD2 and FAD3,which con-trol the last two steps of ALA biosynthesis during seed oil accumulation.In this study,we compared their promoters,coding sequences,and expression levels in three high-ALA inbred lines 2006L,R8Q10,and YH25005,a low-ALA line A28,a low-ALA/high-oleic-acid accession SW,and the wildtype ZS11.The expression levels of most FAD2 and FAD3 homologs in the three high-ALA accessions were higher than those in ZS11 and much higher than those in A28 and SW.The three high-ALA accessions shared similar sequences with the pro-moters and CDSs of BnFAD3.C4 and BnFAD3.A3.In A28 and SW,substitution of three amino acid residues in BnFAD2.A5 and BnFAD2.C5,an absence of BnFAD2.C1 locus,and a 549 bp long deletion on the BnFAD3.A3 promoter were detected.The profile of BnFAD2 mutation in the two low-ALA accessions A28 and SW is different from that reported in previous studies.The mutations in BnFAD3 in the high-ALA accessions are reported for thefirst time.In identifying the sites of these mutations,we provide detailed information to aid the design of mole-cular markers for accelerated breeding schemes.

Secure Transmission of Compressed Medical Image Sequences on Communication Networks Using Motion Vector Watermarking

Medical imaging plays a key role within modern hospital management systems for diagnostic purposes.Compression methodologies are extensively employed to mitigate storage demands and enhance transmission speed,all while upholding image quality.Moreover,an increasing number of hospitals are embracing cloud computing for patient data storage,necessitating meticulous scrutiny of server security and privacy protocols.Nevertheless,considering the widespread availability of multimedia tools,the preservation of digital data integrity surpasses the significance of compression alone.In response to this concern,we propose a secure storage and transmission solution for compressed medical image sequences,such as ultrasound images,utilizing a motion vector watermarking scheme.The watermark is generated employing an error-correcting code known as Bose-Chaudhuri-Hocquenghem(BCH)and is subsequently embedded into the compressed sequence via block-based motion vectors.In the process of watermark embedding,motion vectors are selected based on their magnitude and phase angle.When embedding watermarks,no specific spatial area,such as a region of interest(ROI),is used in the images.The embedding of watermark bits is dependent on motion vectors.Although reversible watermarking allows the restoration of the original image sequences,we use the irreversible watermarking method.The reason for this is that the use of reversible watermarks may impede the claims of ownership and legal rights.The restoration of original data or images may call into question ownership or other legal claims.The peak signal-to-noise ratio(PSNR)and structural similarity index(SSIM)serve as metrics for evaluating the watermarked image quality.Across all images,the PSNR value exceeds 46 dB,and the SSIM value exceeds 0.92.Experimental results substantiate the efficacy of the proposed technique in preserving data integrity.

Optimal mining sequence for coal faces under a bedding slope:insight from landslide prevention

Repetitive mining beneath bedding slopes is identified as a critical factor in geomorphic disturbances, especially landslides and surface subsidence. Prior research has largely concentrated on surface deformation in plains due to multi-seam coal mining and the instability of natural bedding slopes, yet the cumulative impact of different mining sequences on bedding slopes has been less explored. This study combines drone surveys and geological data to construct a comprehensive three-dimensional model of bedding slopes. Utilizing FLAC3D and PFC2D models, derived from laboratory experiments, it simulates stress, deformation, and failure dynamics of slopes under various mining sequences. Incorporating fractal dimension analysis, the research evaluates the stability of slopes in relation to different mining sequences. The findings reveal that mining in an upslope direction minimizes disruption to overlying strata. Initiating extraction from lower segments increases tensile-shear stress in coal pillar overburdens, resulting in greater creep deformation towards the downslope than when starting from upper segments, potentially leading to localized landslides and widespread creep deformation in mined-out areas. The downslope upward mining sequence exhibits the least fractal dimensions, indicating minimal disturbance to both strata and surface. While all five mining scenarios maintain good slope stability under normal conditions, recalibrated stability assessments based on fractal dimensions suggest that downslope upward mining offers the highest stability under rainfall, contrasting with the lower stability and potential instability risks of upslope downward mining. These insights are pivotal for mining operations and geological hazard mitigation in multi-seam coal exploitation on bedding slopes.

Highlighting relationships between sand thicknesses,reservoir-seal pairs and paleobathymetry from a sequence stratigraphic perspective:An example from Tortonian Serravallian deposits,onshore Niger Delta Basin

The utilization of sequence stratigraphic concepts in identifying sands and their spatial continuity in distinct gross depositional settings is key,especially in frontier settings where data paucity is a common challenge.In the Baka field,onshore Niger Delta,detailed reservoir correlation guided by sequence stratigraphic framework analysis showed the distribution of sand and shale units constituting reservoirseal pairs(RSP)correlatable across the field.Within the 3rd-order packages,it is observed that the lowstand systems tract(LST)and highstand systems tract(HST)contain more RSPs and thicker 4th-and 5th-order sands than the transgressive systems tract(TST).In terms of bathymetry,it is noted that irrespective of systems tracts,the RSP Index(RI)decreases from the proximal shallow/inner shelf settings to the more distal outer shelf areas.Amongst all three systems tracts,intervals interpreted as lowstand prograding complexes contain the best developed sands and highest RSP.Sand development within the LSTs has been controlled by a pronounced growth fault regime accompanied by high subsidence and sedimentation rates.This is linked to the basinward migration of the sands during prolonged sea-level fall,creating significant accommodation space for sand deposition.On the other hand,the TSTs known to mark periods of progressive sea-level rise and landward migration of sandy facies,show thinner sands enclosed in much thicker,laterally extensive,and better-preserved deeper marine shales.Interpreted seismic sections indicate intense growth faulting and channelization that influenced the syn-and postdepositional development of the sand packages across the field.The initial timing of deformation of subregional faults in this area coincides with periods of abrupt falls in sea level.This approach could be useful for predicting sand-prone areas in frontier fields as well as possible reservoir-seal parameters required for some aspects of petroleum system analysis and quick-look volume estimation.

Method and Description of Sedimentary System Characterization Based on Sequence Stratigraphy

A Oilfield is the most representative mid to deep oil field in the Bohai Sea, with the main oil bearing intervals being the upper and middle Shahejie-3 sections of the Paleogene Shahejie Formation. By combining well seismic analysis, the middle section of Shahejie-3 is divided into high-level system tract and forced lake retreat system tract, corresponding to the II oil formation and I oil formation, respectively. Using sequence stratigraphy methods, based on seismic profiles and drilling lithological cycles, the high stand system tract is divided into 5 stages of delta progradation. The first and second stages are high angle S-type progradation with large sedimentary thickness, the third stage is oblique progradation, and the fourth and fifth stages are S-oblique composite progradation;By combining seismic data, we characterized the large-scale (8 small-scale) progradation bodies of 5 periods, clarified the distribution characteristics of reservoir planes, and laid the foundation for the later exploration of oilfield potential.

Coseismic Coulomb stress changes induced by a 2020-2021 M_(W)>7.0 Alaska earthquake sequence in and around the Shumagin gap and its influence on the Alaska-Aleutian subduction interface

Three M_(W)>7.0 earthquakes in 2020-2021 occurred in the Shumagin seismic gap and its adjacent area of the Alaska-Aleutian subduction zone,including the Mw7.8 Simeonof thrust earthquake on July 22,2020,the M_(W)7.6 Sand Point strike-slip earthquake on October 19,2020,and the M_(W)8.2 Chignik thrust earthquake on July 29,2021.The spatial and temporal proximity of these three earthquakes prompts us to probe stress-triggering effects among them.Here we examine the coseismic Coulomb stress change imparted by the three earthquakes and their influence on the subduction interface.Our results show that:(1)The Simeonof earthquake has strong loading effects on the subsequent Sand Point and Chignik earthquakes,with the Coulomb stress changes of 3.95 bars and 2.89 bars,respectively.The Coulomb stress change caused by the Sand Point earthquake at the hypocenter of the Chignik earthquake is merely around 0.01 bars,suggesting the negligible triggering effect on the latter earthquake;(2)The triggering effects of the Simeonof,Sand Point,and Chignik earthquakes on aftershocks within three months are not well pronounced because of the triggering rates of 38%,14%,and 43%respectively.Other factors may have played an important role in promoting the occurrence of these aftershocks,such as the roughness of the subduction interface,the complicated velocity structure of the lithosphere,and the heterogeneous prestress therein;(3)The three earthquakes caused remarkable coseismic Coulomb stress changes at the subduction interface nearby these mainshocks,with an average Coulomb stress change of 3.2 bars in the shallow region directly inwards the trench.

Intelligent extraction of road cracks based on vehicle laser point cloud and panoramic sequence images

In light of the limited efficacy of conventional methods for identifying pavement cracks and the absence of comprehensive depth and location data in two-dimensional photographs,this study presents an intelligent strategy for extracting road cracks.This methodology involves the integration of laser point cloud data obtained from a vehicle-mounted system and a panoramic sequence of images.The study employs a vehicle-mounted LiDAR measurement system to acquire laser point cloud and panoramic sequence image data simultaneously.A convolutional neural network is utilized to extract cracks from the panoramic sequence image.The extracted sequence image is then aligned with the laser point cloud,enabling the assignment of RGB information to the vehicle-mounted three dimensional(3D)point cloud and location information to the two dimensional(2D)panoramic image.Additionally,a threshold value is set based on the crack elevation change to extract the aligned roadway point cloud.The three-dimensional data pertaining to the cracks can be acquired.The experimental findings demonstrate that the use of convolutional neural networks has yielded noteworthy outcomes in the extraction of road cracks.The utilization of point cloud and image alignment techniques enables the extraction of precise location data pertaining to road cracks.This approach exhibits superior accuracy when compared to conventional methods.Moreover,it facilitates rapid and accurate identification and localization of road cracks,thereby playing a crucial role in ensuring road maintenance and traffic safety.Consequently,this technique finds extensive application in the domains of intelligent transportation and urbanization development.The technology exhibits significant promise for use in the domains of intelligent transportation and city development.

Influence of Production Sequence of Aluminum Alloy Hot Rolling on Strip Surface Quality

With the intensification of market competition in the aluminum alloy strip processing industry,it is dif-ficult to control the mass production of the same specifications,which is bound to affect the hot rolling production.This paper studied the effect of the hot rolling order of aluminum alloy on the surface quality of strip,such as roll printing,color difference,anodic oxidation,etc.,reasonable discharge sequence and corresponding optimization measures were formulated.

Complete Convergence Properties of the Sums for -mixing Sequences of Random Variables

This paper discusses complete convergence properties of the sums of -mixing random sequences.As a result,we improve the corresponding results of Wu Qunying(2001). And extended the Baum and Katz complete convergence to the case of -mixing random sequences by moment inequality and truncating without necessarily adding any extra conditions.

Strong Laws of Large Numbers for Weighted Sums of Ч-mixing Sequence

In this paper, strong laws of large numbers for weighted sums of ■-mixing sequence are investigated. Our results extend the corresponding results for negatively associated sequence to the case of ■-mixing sequence.

Strong Law of Large Numbers and Complete Convergence for Sequences of -Mixing Random Variables

We give some theorems of strong law of large numbers and complete convergence for sequences of φ-mixing random variables. In particular, Wittmann＇s strong law of large numbers and Teicher＇s strong law of large nnumbers for independent random variables are generalized to the case of φ -minxing random variables.

Some Convergence Results for Sequences of -mixing Random Variables

In this paper, we will present some strong convergence results for sequences of ψ-mixing random variables. The results for sequences of ψ-mixing random variables generalize the corresponding results for independent random variable sequences without any extra conditions.

The Law of the Iterated Logarithm for the Sums of φ-Mixing Sequences with Duple Suffixes

Hu Shuhe gets a sufficient condition on the law of the iterated logarithm for the sums of φ-mixing sequences with duple suffixes. This paper greatly improves his condition.

Complete Convergence for Weighted Sums of φ-mixing Sequence

In this paper, the complete convergence and strong law of large numbers for weighted sums of φ-mixing sequence with different distribution are investigated under some weaker moment conditions. Our results extend ones of independent sequence with identical distribution to the case of φ-mixing sequence with different distribution.