Targeted microbiome metabolomics reveals flaxseed oil supplementation regulated the gut microbiota and farnesoid X receptor pathway in high-fat diet mice

Flaxseed oil(FSO)rich in n-3 polyunsaturated fatty acids(PUFAs)can protect against obesity and insulin resistance,but the underlying mechanism is unknown.An integrative multiomics of the microbiome and targeted metab olomics approach was performed to investigate the possible pathway for flaxseed oil supplementation on reducing serum total cholesterol,triglyceride and epididymal adipose in high-fat diet mice.FSO ameliorated the gut microbial dysbiosis by increasing the community diversity and the abundance of Clostridiales and Ruminococcaceae.These effects were associated with the regulation of bile acid(BAs)in the feces.FSO reduced the concentrations of conjugated BAs,such as cholic acid,tauro-α-murocholic acid,and tauro-ursodesoxycholic acid in feces,which in turn inhibit the intestinal farnesoid X receptor(FXR)-fibroblast growth factor(FGF)15 signaling pathway.Further analysis revealed that FSO activated FXR in the liver and regulated downstream gene expression(SHP,SREBP-1c,and CPT-1a),which promoted lipolysis and inhibited lipogenesis.The results of this study suggest that FSO modulates serum lipid concentrations by regulating the gut microbiota,FXR-FGF15 signaling and BA metabolism.

Dietary supplementation of benzoic acid and essential oils combination enhances intestinal resilience against LPS stimulation in weaned piglets

Background The benefits of combining benzoic acid and essential oils(BAO)to mitigate intestinal impairment during the weaning process have been well established,while the detailed underlying mechanism has not been fully elucidated.Previous research has primarily focused on the reparative effects of BAO on intestinal injury,while neglecting its potential in enhancing intestinal stress resistance.Methods In this study,we investigated the pre-protective effect of BAO against LPS-induced stress using a modified experimental procedure.Piglets were pre-supplemented with BAO for 14 d,followed by a challenge with LPS or saline to collect blood and intestinal samples.Results Our findings demonstrated that BAO supplementation led to significant improvements in piglets’final weight,average daily gain,and feed intake/body gain ratio.Additionally,BAO supplementation positively influenced the composition of intestinal microbiota,increasing beneficial Actinobacteriota and Alloprevotella while reducing harmful Desulfobacterota,Prevotella and Oscillospira.Furthermore,BAO supplementation effectively mitigated oxidative disturbances and inflammatory responses induced by acute LPS challenge.This was evidenced by elevated levels of T-AOC,SOD,and GSH,as well as decreased levels of MDA,TNF-α,and IL-6 in the plasma.Moreover,piglets subjected to LPS challenge and pre-supplemented with BAO exhibited significant improvements in intestinal morphological structure and enhanced integrity,as indicated by restored expression levels of Occludin and Claudin-1 compared to the non-supplemented counterparts.Further analysis revealed that BAO supplementation enhanced the jejunal antioxidative capacity by increasing GSH-Px levels and decreasing MDA levels under the LPS challenge and stimulated the activation of the Nrf2 signaling pathway.Additionally,the reduction of TLR4/NF-κB/MAPK signaling pathways activation and proinflammatory factor were also observed in the jejunal of those piglets fed with BAO.Conclusions In summary,our study demonstrates that pre-supplementation of BAO enhances the anti-stress capacity of weaned piglets by improving intestinal microbiota composition,reinforcing the intestinal barrier,and enhancing antioxidative and anti-inflammatory capabilities.These effects are closely associated with the activation of Nrf2 and TLR4/NF-κB/MAPK signaling pathways.

Geochemical characteristics and exploration significance of ultra-deep Sinian oil and gas from Well Tashen 5,Tarim Basin,NW China

The Well Tashen 5(TS5),drilled and completed at a vertical depth of 9017 m in the Tabei Uplift of the Tarim Basin,NW China,is the deepest well in Asia.It has been producing both oil and gas from the Sinian at a depth of 8780e8840 m,also the deepest in Asia in terms of oil discovery.In this paper,the geochemical characteristics of Sinian oil and gas from the well were investigated and compared with those of Cambrian oil and gas discovered in the same basin.The oil samples,with Pr/Ph ratio of 0.78 and a whole oil carbon isotopic value of31.6‰,have geochemical characteristics similar to those of Ordovician oils from the No.1 fault in the North Shuntuoguole area(also named Shunbei area)and the Middle Cambrian oil from wells Zhongshen 1(ZS1)and Zhongshen 5(ZS5)of Tazhong Uplift.The maturity of light hydrocarbons,diamondoids and aromatic fractions all suggest an approximate maturity of 1.5%e1.7%Ro for the samples.The(4-+3-)methyldiamantane concentration of the samples is 113.5 mg/g,indicating intense cracking with a cracking degree of about 80%,which is consistent with the high bottom hole temperature(179℃).The Sinian gas samples are dry with a dryness coefficient of 0.97.The gas is a mixture of kerogen-cracking gas and oil-cracking gas and has Ro values ranging between 1.5%and 1.7%,and methane carbon isotopic values of41.6‰.Based on the equivalent vitrinite reflectance(R_(eqv)=1.51%e1.61%)and the thermal evolution of source rocks from the Cambrian Yu'ertusi Formation of the same well,it is proposed that the Sinian oil and gas be mainly sourced from the Cambrian Yu'ertusi Formation during the Himalayan period but probably also be joined by hydrocarbon of higher maturity that migrated from other source rocks in deeper formations.The discovery of Sinian oil and gas from Well TS5 suggests that the ancient ultra-deep strata in the northern Tarim Basin have the potential for finding volatile oil or condensate reservoirs.

Silicone oil as a corneal lubricant to reduce corneal edema and improve visualization during

AIM:To evaluate the efficacy and safety of silicone oil(SO)as a corneal lubricant to improve visualization during vitrectomy.METHODS:Patients who underwent vitreoretinal surgery were divided into two groups.Group 1 was operated on with initial SO(Oxane 5700)as a corneal lubricant.Group 2 was operated on with initial lactated ringer’s solution(LRS)and then replaced with SO as required.Fundus clarity was scored during the surgery.Fluorescein staining was performed to determine the damage to corneal epithelium.RESULTS:Totally 114 eyes of 114 patients were included.Single SO use maintained a clear cornea and provided excellent visualization of surgical image.In group 1,the fundus clarity was grade 3 in 41/45 eyes and grade 2 in 4/45 eyes.In group 2,corneal edema frequently occurred after initial LRS use.The fundus clarity was grade 3 in 19/69 eyes,2 in 37/69 eyes and 1 in 13/69 eyes(P<0.05).SO was applied in 29 eyes of initial LRS use with subsequent corneal edema,which eliminated the corneal edema in 26 eyes.Corneal fluorescein staining score in group 1 was 0 in 28 eyes,1 in 11 eyes and 2 in 6 eyes,and 40,20 and 9,respectively,in group 2(all P>0.05).CONCLUSION:The use of SO as a corneal lubricant is effective and safe for preserving and improving corneal clarity and providing clear surgical field during vitrectomy.

Molecular Mechanism and Molecular Design of Lubricating Oil Antioxidants

To overcome the limitations of traditional experimental“trial and error”methods in lubricant additive design,a new molecular design method based on molecular structure parameters is established here.The molecular mechanism of the antioxidant reaction of hindered phenol,diphenylamine,and alkyl sulfide are studied via molecular simulations.Calculation results show that the strong electron-donating ability and high hydrogen-donating activity of the antioxidant molecule and the low hydrogen-abstracting activity of free radicals formed after dehydrogenation are the internal molecular causes of the shielding of phenol and diphenylamine from scavenging peroxy free radicals,and the strong electron-donating ability is the internal molecular cause of the high activity of thioether in decomposing alkyl hydrogen peroxide.Based on this antioxidant molecular mechanism,a molecular design rule of antioxidant is proposed,namely“high EHOMO,large Q(S),low bond dissociation energy BDE(O—H)and BDE(N—H)”.Two new antioxidants,PAS-I and PAS-II,are designed and prepared by chemical bonding of hindered phenol,diphenylamine,and sulfur atoms.Experimental results show that these antioxidants both have excellent antioxidant effects in lubricating oil,and that PAS-II is the superior antioxidant,consistent with theoretical predictions.

Risk assessment of oil and gas investment environment in countries along the Belt and Road Initiative

With the implementation of the Belt and Road Initiative, China is deepening its cooperation in oil and gas resources with countries along the Initiative. In order to better mitigate risks and enhance the safety of investments, it is of significant importance to research the oil and gas investment environment in these countries for China's overseas investment macro-layout. This paper proposes an indicator system including 27 indicators from 6 dimensions. On this basis, game theory models combined with global entropy method and analytic hierarchy process are applied to determine the combined weights, and the TOPSIS-GRA model is utilized to assess the risks of oil and gas investment in 76 countries along the Initiative from 2014 to 2021. Finally, the GM(1,1) model is employed to predict risk values for 2022-2025. In conclusion, oil and gas resources and political factors have the greatest impact on investment environment risk, and 12 countries with greater investment potential are selected through cluster analysis in conjunction with the predicted results. The research findings may provide scientific decisionmaking recommendations for the Chinese government and oil enterprises to strengthen oil and gas investment cooperation with countries along the Belt and Road Initiative.

Identification and evaluation of shale oil micromigration and its petroleum geological significance

Taking the Lower Permian Fengcheng Formation shale in Mahu Sag of Junggar Basin,NW China,as an example,core observation,test analysis,geological analysis and numerical simulation were applied to identify the shale oil micro-migration phenomenon.The hydrocarbon micro-migration in shale oil was quantitatively evaluated and verified by a self-created hydrocarbon expulsion potential method,and the petroleum geological significance of shale oil micro-migration evaluation was determined.Results show that significant micro-migration can be recognized between the organic-rich lamina and organic-poor lamina.The organic-rich lamina has strong hydrocarbon generation ability.The heavy components of hydrocarbon preferentially retained by kerogen swelling or adsorption,while the light components of hydrocarbon were migrated and accumulated to the interbedded felsic or carbonate organic-poor laminae as free oil.About 69% of the Fengcheng Formation shale samples in Well MY1 exhibit hydrocarbon charging phenomenon,while 31% of those exhibit hydrocarbon expulsion phenomenon.The reliability of the micro-migration evaluation results was verified by combining the group components based on the geochromatography effect,two-dimension nuclear magnetic resonance analysis,and the geochemical behavior of inorganic manganese elements in the process of hydrocarbon migration.Micro-migration is a bridge connecting the hydrocarbon accumulation elements in shale formations,which reflects the whole process of shale oil generation,expulsion and accumulation,and controls the content and composition of shale oil.The identification and evaluation of shale oil micro-migration will provide new perspectives for dynamically differential enrichment mechanism of shale oil and establishing a“multi-peak model in oil generation”of shale.

Synergistic anionic/zwitterionic mixed surfactant system with high emulsification efficiency for enhanced oil recovery in low permeability reservoirs

Emulsification is one of the important mechanisms of surfactant flooding. To improve oil recovery for low permeability reservoirs, a highly efficient emulsification oil flooding system consisting of anionic surfactant sodium alkyl glucosyl hydroxypropyl sulfonate(APGSHS) and zwitterionic surfactant octadecyl betaine(BS-18) is proposed. The performance of APGSHS/BS-18 mixed surfactant system was evaluated in terms of interfacial tension, emulsification capability, emulsion size and distribution, wettability alteration, temperature-resistance and salt-resistance. The emulsification speed was used to evaluate the emulsification ability of surfactant systems, and the results show that mixed surfactant systems can completely emulsify the crude oil into emulsions droplets even under low energy conditions. Meanwhile,the system exhibits good temperature and salt resistance. Finally, the best oil recovery of 25.45% is achieved for low permeability core by the mixed surfactant system with a total concentration of 0.3 wt%while the molar ratio of APGSHS:BS-18 is 4:6. The current study indicates that the anionic/zwitterionic mixed surfactant system can improve the oil flooding efficiency and is potential candidate for application in low permeability reservoirs.

Miscibility of light oil and flue gas under thermal action

The miscibility of flue gas and different types of light oils is investigated through slender-tube miscible displacement experiment at high temperature and high pressure.Under the conditions of high temperature and high pressure,the miscible displacement of flue gas and light oil is possible.At the same temperature,there is a linear relationship between oil displacement efficiency and pressure.At the same pressure,the oil displacement efficiency increases gently and then rapidly to more than 90% to achieve miscible displacement with the increase of temperature.The rapid increase of oil displacement efficiency is closely related to the process that the light components of oil transit in phase state due to distillation with the rise of temperature.Moreover,at the same pressure,the lighter the oil,the lower the minimum miscibility temperature between flue gas and oil,which allows easier miscibility and ultimately better performance of thermal miscible flooding by air injection.The miscibility between flue gas and light oil at high temperature and high pressure is more typically characterized by phase transition at high temperature in supercritical state,and it is different from the contact extraction miscibility of CO_(2) under conventional high pressure conditions.

Discovery and inspiration of large-and medium-sized glutenite-rich oil and gas fields in the eastern South China Sea:An example from Paleogene Enping Formation in Huizhou 26 subsag,Pearl River Mouth Basin

Based on the practice of oil and gas exploration in the Huizhou Sag of the Pearl River Mouth Basin,the geochemical indexes of source rocks were measured,the reservoir development morphology was restored,the rocks and minerals were characterized microscopically,the measured trap sealing indexes were compared,the biomarker compounds of crude oil were extracted,the genesis of condensate gas was identified,and the reservoir-forming conditions were examined.On this basis,the Paleogene Enping Formation in the Huizhou 26 subsag was systematically analyzed for the potential of oil and gas resources,the development characteristics of large-scale high-quality conglomerate reservoirs,the trapping effectiveness of faults,the hydrocarbon migration and accumulation model,and the formation conditions and exploration targets of large-and medium-sized glutenite-rich oil and gas fields.The research results were obtained in four aspects.First,the Paleogene Wenchang Formation in the Huizhou 26 subsag develops extensive and thick high-quality source rocks of semi-deep to deep lacustrine subfacies,which have typical hydrocarbon expulsion characteristics of"great oil generation in the early stage and huge gas expulsion in the late stage",providing a sufficient material basis for hydrocarbon accumulation in the Enping Formation.Second,under the joint control of the steep slope zone and transition zone of the fault within the sag,the large-scale near-source glutenite reservoirs are highly heterogeneous,with the development scale dominated hierarchically by three factors(favorable facies zone,particle component,and microfracture).The(subaqueous)distributary channels near the fault system,with equal grains,a low mud content(<5%),and a high content of feldspar composition,are conducive to the development of sweet spot reservoirs.Third,the strike-slip pressurization trap covered by stable lake flooding mudstone is a necessary condition for oil and gas preservation,and the NE and nearly EW faults obliquely to the principal stress have the best control on traps.Fourth,the spatiotemporal configuration of high-quality source rocks,fault transport/sealing,and glutenite reservoirs controls the degree of hydrocarbon enrichment.From top to bottom,three hydrocarbon accumulation units,i.e.low-fill zone,transition zone,and high-fill zone,are recognized.The main area of the channel in the nearly pressurized source-connecting fault zone is favorable for large-scale hydrocarbon enrichment.The research results suggest a new direction for the exploration of large-scale glutenite-rich reservoirs in the Enping Formation of the Pearl River Mouth Basin,and present a major breakthrough in oil and gas exploration.

Quantification of the adulteration concentration of palm kernel oil in virgin coconut oil using near-infrared hyperspectral imaging

The adulteration concentration of palm kernel oil(PKO)in virgin coconut oil(VCO)was quantified using near-infrared(NIR)hyperspectral imaging.Nowadays,some VCO is adulterated with lower-priced PKO to reduce production costs,which diminishes the quality of the VCO.This study used NIR hyperspectral imaging in the wavelength region 900-1,650 nm to create a quantitative model for the detection of PKO contaminants(0-100%)in VCO and to develop predictive mapping.The prediction equation for the adulteration of VCO with PKO was constructed using the partial least squares regression method.The best predictive model was pre-processed using the standard normal variate method,and the coefficient of determination of prediction was 0.991,the root mean square error of prediction was 2.93%,and the residual prediction deviation was 10.37.The results showed that this model could be applied for quantifying the adulteration concentration of PKO in VCO.The prediction adulteration concentration mapping of VCO with PKO was created from a calibration model that showed the color level according to the adulteration concentration in the range of 0-100%.NIR hyperspectral imaging could be clearly used to quantify the adulteration of VCO with a color level map that provides a quick,accurate,and non-destructive detection method.

ω-3 Rich Tetracarpidum conophorum Oil Exhibits Better Prevention Effects for Cardiovascular Risk Factors than Corn Oil in Adult of Albinos Wistar Male Rats

Cardiovascular diseases are serious pathologies that affect an increasing number of people. Several preventive measures are generally used, including supplementing of oils in foods. Our objective was to compare the effects of Tetracarpidum conophorum oil (TC) and corn oil (CO) on serum lipid profiles of normal male rats. 42 Wistar rats were divided into 7 groups. Diets included TC oil (groups TC5, TC10 and TC20) and corn oil (groups CO5, CO10 and CO20) in proportions of 5%, 10% and 20%, with a control group (T). After 5 weeks of feeding, several parameters were measured during and after the study, including body weight, food intake and organ weights (kidney, liver and fat). Lipid profiles (total cholesterol, TG, HDL and LDL), glucose and protein levels were measured in the serum. The increase in body mass was inversely proportional to the amount of oil in the food. The decrease in body mass and adiposomatic index of group TC10 was significant (p < 0.05) compared with the other groups. The lowest glycaemia (64.17 ± 5.14 mg/dl) was noted with the diet containing 20% TC oil. A significant reduction in total cholesterol, LDL fraction and blood triglycerides was observed in the groups supplemented with TC and corn oils compared to controls. Results were also more beneficial for the TC10 group. HDL-cholesterol levels were significantly higher (p < 0.05) in the oil-supplemented groups than in the control group. Castelli’s risk indices decrease significantly (p < 0.05) with increasing oil content for TC. The oils had no impact on blood protein contents. One can conclude that a diet containing 10% crude oil from TC kernels could prevent or alleviate cardiovascular diseases and glycemia.

Geological characteristics of unconventional tight oil reservoir (10^(9) t): A case study of Upper Cretaceous Qingshankou Formation, northern Songliao Basin, NE China

The Daqing exploration area in the northern Songliao Basin has great potential for unconventional oil and gas resources,among which the total resources of tight oil alone exceed 109 t and is regarded as an important resource base of Daqing oilfield.After years of exploration in the Qijia area,Songliao Basin,NE China,tight oil has been found in the Upper Cretaceous Qingshankou Formation.To work out tight oil’s geological characteristics,taking tight oil in Gaotaizi oil layers of the Upper Cretaceous Qingshankou Formation in northern Songliao Basin as an example,this paper systematically analyzed the geological characteristics of unconventional tight oil in Gao3 and Gao4 layers of the Qijia area,based on the data of the geological survey,well drilling journey,well logging,and test.It is that three sets of hydrocarbon source rocks(K2qn1,K2qn2+3,and K2n1)develop in the examined area,and exhibit excellent type I and II kerogens,high organic matter abundance,and moderate maturity.The reservoir is generally composed of thin-bedded mudstone,siltstone,and sandstone,and presents poor porosity(average 8.5 vol.%)and air permeability(average 4 mD).The main reservoir space primarily includes intergranular pores,secondary soluble pores,and intergranular soluble pores.Three types of orifice throats were identified,namely fine throat,extra-fine throat,and micro-fine throat.The siltstone is generally oil-bearing,the reservoirs with slime and calcium become worse oil-bearing,and the mudstone has no obvious oil-bearing characteristics.The brittleness indices of the sandstone in the tight oil reservoir range from 40%to 60%,and those of the mudstone range from 40%to 45%,indicating a better brittleness of the tight oil reservoir.Based on the study of typical core hole data,this paper gives a comprehensive evaluation of the properties of the tight oil and establishes a tight oil single well composite bar chart as well as the initial evaluation system with the core of properties in the tight oil reservoir.This study has theoretical guiding significance and practical application value for tight oil exploration and evaluation in the Qijia area.

Influence of the Extraction Process on the Characteristics of Romanian Mountain Walnut Oil

The objective of this work is to extract walnut oil using various processes in order to compare the influence on the nature of the components extracted, and thus identify the areas of potential use. We carried out the extractions by mechanical process, thanks to a press in reduced model provided with a worm. We obtained cold extracted oil whose characteristics slightly diverge from extra virgin oil found in shops in Romania, but its composition is similar. We were also able to extract by chemical process using two methods, Folch and Soxhlet. Commercially available table walnut oils are only cold extracted to avoid the presence of solvents. Those are difficult to remove and strongly oxidize the oil. Currently, consumers appreciate walnut oil for its taste and nutritional qualities. In nutrition, this oil is put forward for its composition rich in polyunsaturated fatty acids, which are needed for human body. Food supplements made from walnut oil are available today. For the moment, this is the only use of walnut oil. Indeed, there are some studies on other fields of application, but they remain in the field of research and nothing has yet been commercialized. In this present study, we compared the chemical and physical properties of cold-extracted oil with the solvent extraction of walnut kernel originating from the mountain region of Rumania. The cold extracted oil has a high content of polyunsaturated fatty acids (63%) and monounsaturated fatty acids (30%), a very low level of saturated fatty acid (7%) and no content of linolenic acid. The Soxhlet and Folch methods produced slightly different oils with increased amounts of minor components, which changes their characteristic. Even when solvent-extracted oils do not meet the standard criteria imposed by the Codex Alimentarius, they offer a possible use in the fields of food, cosmetics industries and biomedicine.

Reactions of Oil Palm (Elaeis guineensis Jacq) Progenies to Fusarium Wilt Disease Caused by Fusarium oxysporum f.sp. Elaeidis under Natural Infection

The oil palm (Elaeisguineensis Jacq) is used worldwide in commercial agriculture for the production of palm oil, palm kernel oil and palm wine. It produces more oil per plant than any other oil-producing crop in the world. Production is constrained by several factors among which pests/diseases are of utmost importance. Vascular wilt (VW) caused by Fusarium oxysporum is the most devastating disease infecting this crop. Its soil-borne ecology has made the use of fungicides to manage this disease too expensive and inpragmatic. There is need for concerted research in the breeding and selection of wilt-tolerant progenies as an essential step in the management of Fusarium wilt disease. The study aims to assess the incidence and severity of vascular wilt among tested oil palm progenies, to evaluate the reduction in yield caused by the disease in the susceptible progenies and to identify the wilt-tolerant, high-yielding progenies. The study was carried out at Pamol Plantations Limited (PPL) in Ndian Estate (Ndian Division), in the Southwest Region of Cameroon. Three field trials were evaluated for tolerance/susceptibility to Fusarium wilt. Each trial consisted of 15 oil palm progenies replicated 4 times. Each progeny had 25 oil palm stands in each replicate. Hence, a total of 1500 oil palm stands were assessed. The experimental design was a randomized complete block (RCB) with trial codes: Trial 2001/1, planted in 2001;Trial 2001/2, planted in 2002;Trial 2001/3, planted in 2003. Each trail had an area of 12 ha, with a plant density of 143 palms·ha−1. Wilt incidence, severity, index, and yield were evaluated on 45 progenies from the 3 trails after identifying Fusarium oxysporum from oil palm plant part. Data was subjected to analysis of variance, Fischer’s least significant difference test (LSD) for mean separation. Identification of Fusarium was based on descriptive analysis. Incidence of VW in the 3 trials ranged from 1% - 39%. Also, 45% of infected plants were from progeny 676 while 1% was from progenies 689, 693, 694 and 710. Disease severity was from 0.9 in progeny 686 to 4.55 in 676. Wilt index ranged from 131 for progeny 694 and 710 to 495 for progenies 705. Out of the 45 progenies evaluated, 27 were tolerant (1 < 100) and 18 susceptible (1 ≥ 100). Within the tolerant progenies, 4 were significant (1 < 20) while 5 out of 18 were significantly susceptible (1 ≥ 185). Mean yield reduction of the susceptible progenies was 34.8% while in the tolerant progenies, it increased by 9.5% when compared to their controls. Progenies 702, 703 and 709 are recommended for planting based on the level of tolerance to Fusarium wilt disease and yield.

CO_(2)flooding in shale oil reservoir with radial borehole fracturing for CO_(2)storage and enhanced oil recovery

This study introduces a novel method integrating CO_(2)flooding with radial borehole fracturing for enhanced oil recovery and CO_(2)underground storage,a solution to the limited vertical stimulation reservoir volume in horizontal well fracturing.A numerical model is established to investigate the production rate,reservoir pressure field,and CO_(2)saturation distribution corresponding to changing time of CO_(2)flooding with radial borehole fracturing.A sensitivity analysis on the influence of CO_(2)injection location,layer spacing,pressure difference,borehole number,and hydraulic fractures on oil production and CO_(2)storage is conducted.The CO_(2)flooding process is divided into four stages.Reductions in layer spacing will significantly improve oil production rate and gas storage capacity.However,serious gas channeling can occur when the spacing is lower than 20 m.Increasing the pressure difference between the producer and injector,the borehole number,the hydraulic fracture height,and the fracture width can also increase the oil production rate and gas storage rate.Sensitivity analysis shows that layer spacing and fracture height greatly influence gas storage and oil production.Research outcomes are expected to provide a theoretical basis for the efficient development of shale oil reservoirs in the vertical direction.

Purification of Produced Water from a Sour Oilfield in South Kuwait. 1. Oil-Water Separation and Industrial Salt Production

Produced water from an oil extraction site in South Kuwait was sampled after primary oil – water separation had been carried out. The produced water was filtered through a mixture of activated charcoal and esterified cellulosic material gained from spent coffee grounds as a tertiary adsorption treatment. The earth-alkaline metal ions and heavy metals were separated from the de-oiled produced water by addition of either sodium or potassium hydroxide in the presence of carbon dioxide or by direct addition of solid sodium carbonate. The resulting filtrate gave salt of industrial purity upon selective crystallization on evaporation.

A review:Health benefits and physicochemical characteristics of blended vegetable oils

Oil blending is the method of choice used worldwide to improve oxidative stability and nutritional value.There is no such edible oil/fat that meets all the recommendations from the health point of view.The fatty acid composition of vegetable oils decides the fate of the oil.Pure single oil is unable to provide a balanced amount of fatty acids(FAs)required/recommended on a daily intake basis.Blending oils/fats is an appropriate procedure of physically mixing multiple oils in suitable proportions which may provide functional lipids with improved antioxidant potential and desirable physical and chemical properties.This review piled up the accessible data on the blending of diverse oils/fats in the combination of binary,ternary,quaternary,or other types of oils into a single blended oil.Blending can be found very convincing towards appropriate FA profile,enhancement in physicochemical characteristics,and augmented stability for the period of storage or when used as cooking/frying processes which could ultimately serve as an effectual dietary intervention towards the health protectiveness.

Geologic characteristics,exploration and production progress of shale oil and gas in the United States:An overview

We present a systematic summary of the geological characteristics,exploration and development history and current state of shale oil and gas in the United States.The hydrocarbon-rich shales in the major shale basins of the United States are mainly developed in six geological periods:Middle Ordovician,Middle-Late Devonian,Early Carboniferous(Middle-Late Mississippi),Early Permian,Late Jurassic,and Late Cretaceous(Cenomanian-Turonian).Depositional environments for these shales include intra-cratonic basins,foreland basins,and passive continental margins.Paleozoic hydrocarbon-rich shales are mainly developed in six basins,including the Appalachian Basin(Utica and Marcellus shales),Anadarko Basin(Woodford Shale),Williston Basin(Bakken Shale),Arkoma Basin(Fayetteville Shale),Fort Worth Basin(Barnett Shale),and the Wolfcamp and Leonardian Spraberry/Bone Springs shale plays of the Permian Basin.The Mesozoic hydrocarbon-rich shales are mainly developed on the margins of the Gulf of Mexico Basin(Haynesville and Eagle Ford)or in various Rocky Mountain basins(Niobrara Formation,mainly in the Denver and Powder River basins).The detailed analysis of shale plays reveals that the shales are different in facies and mineral components,and"shale reservoirs"are often not shale at all.The United States is abundant in shale oil and gas,with the in-place resources exceeding 0.246×10^(12)t and 290×10^(12)m^(3),respectively.Before the emergence of horizontal well hydraulic fracturing technology to kick off the"shale revolution",the United States had experienced two decades of exploration and production practices,as well as theory and technology development.In 2007-2023,shale oil and gas production in the United States increased from approximately 11.2×10^(4)tons of oil equivalent per day(toe/d)to over 300.0×10^(4)toe/d.In 2017,the shale oil and gas production exceeded the conventional oil and gas production in the country.In 2023,the contribution from shale plays to the total U.S.oil and gas production remained above 60%.The development of shale oil and gas has largely been driven by improvements in drilling and completion technologies,with much of the recent effort focused on“cube development”or“co-development”.Other efforts to improve productivity and efficiency include refracturing,enhanced oil recovery,and drilling of“U-shaped”wells.Given the significant resources base and continued technological improvements,shale oil and gas production will continue to contribute significant volumes to total U.S.hydrocarbon production.

Experiment of dynamic seepage of tight/shale oil under matrix fracture coupling

A physical simulation method with a combination of dynamic displacement and imbibition was established by integrating nuclear magnetic resonance(NMR)and CT scanning.The microscopic production mechanism of tight/shale oil in pore throat by dynamic imbibition and the influencing factors on the development effect of dynamic imbibition were analyzed.The dynamic seepage process of fracking-soaking-backflow-production integration was simulated,which reveals the dynamic production characteristics at different development stages and their contribution to enhancing oil recovery(EOR).The seepage of tight/shale reservoirs can be divided into three stages:strong displacement and weak imbibition as oil produced rapidly by displacement from macropores and fractures,weak displacement and strong imbibition as oil produced slowly by reverse imbibition from small pores,and weak displacement and weak imbibition at dynamic equilibrium.The greater displacement pressure results in the higher displacement recovery and the lower imbibition recovery.However,if the displacement pressure is too high,the injected water is easy to break through the front and reduce the recovery degree.The higher the permeability,the greater the imbibition and displacement recovery,the shorter the time of imbibition balance,and the higher the final recovery.The fractures can effectively increase the imbibition contact area between matrix and water,reduce the oil-water seepage resistance,promote the oil-water displacement between matrix and fracture,and improve the oil displacement rate and recovery of the matrix.The soaking after fracturing is beneficial to the imbibition replacement and energy storage of the fluid;also,the effective use of the carrying of the backflow fluid and the displacement in the mining stage is the key to enhancing oil recovery.