To avoid carbonate precipitation for CO_(2) electrolysis,developing CO_(2) conversion in an acid electrolyte is viewed as an ultimately challenging technology.In Nature,Xia et al.recently explored a proton-exchange me...To avoid carbonate precipitation for CO_(2) electrolysis,developing CO_(2) conversion in an acid electrolyte is viewed as an ultimately challenging technology.In Nature,Xia et al.recently explored a proton-exchange membrane system for reducing CO_(2) to formic acid with a Pb±Pb SO_(4) composite catalyst derived from waste lead-acid batteries based on the lattice carbon activation mechanism.Up to 93%Faradaic efficiency was realized when formic acid was produced by this technology.展开更多
Oxidized and acidic mining discharges(pH between 4 and 5)from the Perkoa zinc mine in Burkina Faso consisting of waste rock,crusher rejects and mining tailings were sampled and used for neutralization tests with calca...Oxidized and acidic mining discharges(pH between 4 and 5)from the Perkoa zinc mine in Burkina Faso consisting of waste rock,crusher rejects and mining tailings were sampled and used for neutralization tests with calcareous dolomite(for six months).The mining discharges and the calcareous dolomite were previously characterized.Columns of 60 cm length and 15 cm diameter were used for the tests.Then,2.62 kg of calcareous dolomite was deposited on the mining discharges in each column.Two liters of deionized water are added every 15 days to each column.Samples were taken at the outlet of the columns at time intervals and then analyzed to assess the evolution of pH,electrical conductivity and contents of PHEs(Potentially Harmful Elements)over time.Following the neutralization tests on solid mining discharges,neutralization tests were also carried out on acid leachates resulting from the oxidation of these same solid mining discharges with calcareous dolomite.The results show that calcareous dolomite is very effective for the neutralization of SR(Sterile Discharges)and the precipitation of PHEs contained therein.It is also effective in buffering acidic effluents from these sterile discharges.As for other mining discharges(crusher rejects and mining residues),although calcareous dolomite is not effective in neutralizing these oxidized and acidic solid discharges,it is however effective in precipitating PHEs such as arsenic and lead.Calcareous dolomite is also effective in buffering acid leachates from these oxidized and acidic mining discharges(crusher rejects and mining tailings).展开更多
In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using t...In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using two-fold dilutions of serum and 100 TCID50 of the virus. The 62 sera samples of giant pandas, which were gathered from zoos and reserve region of Sichuan Province, China were detected. The neutralization antibody titer of 1:4 was recognized as the positive criterion, 8 sera samples were detected to be positive, and the positive rate was 12.9%. The titers of neutralizing antibody ranged from 1:8 to 1:32. It was the first comprehensive investigation on neutralization antibodies against CCV in giant panda population in China. The results of study showed that the infection of CCV in giant panda population was universal, which has posed a threat to the health of giant panda. Therefore, it is incumbent on us to study safe and effective vaccines to protect giant panda against CCV infection.展开更多
The CD4 binding site(CD4bs) of envelope glycoprotein(Env) is an important conserved target for anti-human immunodeficiency virus type 1(HIV-1) neutralizing antibodies. Neutralizing monoclonal antibodies IgG1 b12(b12) ...The CD4 binding site(CD4bs) of envelope glycoprotein(Env) is an important conserved target for anti-human immunodeficiency virus type 1(HIV-1) neutralizing antibodies. Neutralizing monoclonal antibodies IgG1 b12(b12) could recognize conformational epitopes that overlap the CD4 bs of Env. Different virus strains, even derived from the same individual, showed distinct neutralization susceptibility to b12. We examined the key amino acid residues affecting b12 neutralization susceptibility using single genome amplification and pseudovirus neutralization assay. Eleven amino acid residues were identified that affect the sensitivity of Env to b12. Through site-directed mutagenesis, an amino acid substitution at position 182 in the V2 region of Env was confirmed to play a key role in regulating the b12 neutralization susceptibility. The introduction of V182 L to a resistant strain enhanced its sensitivity to b12 more than twofold. Correspondingly, the introduction of L182 V to a sensitive strain reduced its sensitivity to b12 more than tenfold. Amino acid substitution at positions 267 and 346 could both enhance the sensitivity to b12 more than twofold. However, no additive effect was observed when the three site mutageneses were introduced into the same strain, and the sensitivity was equivalent to the single V182 L mutation. CRF07_BC is a major circulating recombinant form of HIV-1 prevalent in China. Our data may provide important information for understanding the molecular mechanism regulating the neutralization susceptibility of CRF07_BC viruses to b12 and may be helpful for a vaccine design targeting the CD4 bs epitopes.展开更多
This paper analyzed the current situation and development trends of energy consumption and carbon emissions,and the current situation and development trend of coal consumption in China.In the context of recently estab...This paper analyzed the current situation and development trends of energy consumption and carbon emissions,and the current situation and development trend of coal consumption in China.In the context of recently established carbon peak and carbon neutralization targets,this paper put forward the main problems associated with the green and low-carbon development and utilization of coal.Five key technological innovation directions in mining were proposed,including green coal development,intelligent and efficient mining,low-carbon utilization and conversion of coal,energy conservation and emission reduction,carbon capture,utilization and storage(CCUS).Focusing on the above technological innovation directions,it is suggested to carry out three basic theories,including the theory of green efficient intelligent mining,clean and low-carbon utilization and transformation of coal,and CCUS.Meanwhile,it is proposed to develop 12 key technologies,including green coal mining and ecological environment protection,efficient coal mining and intelligent mine construction,key technologies and equipment for efficient coal processing,underground coal gasification and mining,ultra-high parameter and ultra-supercritical power generation technology,intelligent and flexible coal-fired power generation technology,new power cycle coal-fired power generation technology,the development of coal-based special fuels,coal-based bulk and specialty chemicals,energy conservation and consumption reduction,large-scale and low-cost carbon capture,CO_(2) utilization and storage.Finally,necessary measures from the governmental perspective were also proposed.展开更多
To achieve the neutralization control requirements of the radio-frequency(RF)ion microthruster(μRIT)in the‘Taiji-1’satellite mission,we proposed an active neutralization control solution that is based on the carbon...To achieve the neutralization control requirements of the radio-frequency(RF)ion microthruster(μRIT)in the‘Taiji-1’satellite mission,we proposed an active neutralization control solution that is based on the carbon nanotube field emission technology.The carbon nanotube field emission neutralizer(CNTN)has the characteristics of light weight,small size,and propellantless,which is especially suitable for the neutralization control tasks of ion microthrusters.The Institute of Mechanics,Chinese Academy of Sciences,in collaboration with Tsinghua University,has successfully developed a CNTN to meet mission requirements.On the ground,the feasibility of cooperation working betweenμRIT and CNTN was fully verified,as well as the simulation and experimental study of neutralization control strategy,which finally passed the engineering assessment test.Since the launch of‘Taiji-1’satellite on 31 August,2019,the RF ion micropropulsion system has successfully completed nearly one hundred test missions in space.The test results indicate that CNTN does not have performance degradation,and the neutralization control strategy is effective.展开更多
The neutralizing activities of eight monoclonal antibodies (MAbs) against white spot syndrome virus (WSSV) (2D2, 2B2,1D2, 1D5, 1C2, 4A1, 6A4 and 6B4) were analyzed by in vivo experiments. Gills from WSSV-infecte...The neutralizing activities of eight monoclonal antibodies (MAbs) against white spot syndrome virus (WSSV) (2D2, 2B2,1D2, 1D5, 1C2, 4A1, 6A4 and 6B4) were analyzed by in vivo experiments. Gills from WSSV-infected shrimp were homogenized and ten-fold serially diluted by PBS, and then incubated with MAbs (hybridoma culture supernatant), respectively. The mixture of WSSV and MAbs were injected into crayfish (Procambarus clarkii). After challenge, the death rates of crayfish were counted to determine the neutralizing activities of MAbs. At the same time, the mixture of myeloma culture supernatant and WSSV or PBS was served as positive or negative control, respectively. The results showed that, at each virus dilution, the mean time to death of the crayfish injected with MAb-treated virus was significantly longer than that in the positive control, though they all showed 100% mortality within 25 d, and meanwhile, few crayfish died in the negative control. Among the eight MAbs, 2D2, 2B2, 1D2 and 1D5, especially the former two, delayed the mortality significantly, and 1 C2, 4A1 and 6A4 delayed the mortality as well but not so efficiently, while MAb 6IM was efficient only when the virus concentration increased. The results indicated that the anti-WSSV MAbs can neutralize WSSV in different virus dilutions.展开更多
This study described a regime map for dry neutralization agglomeration. Based on the map, the effects of selected key parameters, such as ingredient composition, operation temperature, agitation speed, and size of Na_...This study described a regime map for dry neutralization agglomeration. Based on the map, the effects of selected key parameters, such as ingredient composition, operation temperature, agitation speed, and size of Na_2CO_3 particles, were investigated using a laboratory-scale mixer, and properties of the agglomeration product were analyzed, including particle size distribution, Hunter color, and flowability. Torque curves evolving during the process were correlated with the system flowability. Three distinguishable regimes were indicated, dry, wet, and transitional, and the agitation speed was found to have a different influence on the agglomeration process for the three regimes. Furthermore, the influence of temperature on reactive agglomeration significantly differed from that in agglomeration processes in which the binder was non-reactive.展开更多
Control of pH neutralization processes is challenging in the chemical process industry because of their inherent strong nonlinearity. In this paper, the model algorithmic control (MAC) strategy is extended to nonlinea...Control of pH neutralization processes is challenging in the chemical process industry because of their inherent strong nonlinearity. In this paper, the model algorithmic control (MAC) strategy is extended to nonlinear processes using Hammerstein model that consists of a static nonlinear polynomial function followed in series by a linear impulse response dynamic element. A new nonlinear Hammerstein MAC algorithm (named NLH-MAC) is presented in detail. The simulation control results of a pH neutralization process show that NLH-MAC gives better control performance than linear MAC and the commonly used industrial nonlinear propotional plus integral plus derivative (PID) controller. Further simulation experiment demonstrates that NLH-MAC not only gives good control response, but also possesses good stability and robustness even with large modeling errors.展开更多
Bauxite residue deposit area(BRDA)is a typical abandoned mining wasteland representing extreme hostile environment with increased alkalinity.Microbially-driven neutralization of bauxite residue,based on the microbial ...Bauxite residue deposit area(BRDA)is a typical abandoned mining wasteland representing extreme hostile environment with increased alkalinity.Microbially-driven neutralization of bauxite residue,based on the microbial acid producing metabolisms,is a novel strategy for achieving rapid pH neutralization and thus improving its environmental outcomes.The hypothesis was that these extreme conditions promote microbial communities which are capable of novel ecologically relevant functions.Several alkaliphilic acid producing bacteria were isolated in this study.One strain was selected for its superior growth pattern and acid metabolism(termed EEEL02).Based on the phylogenetic analysis,this strain was identified as Bacillus thuringiensis.The optimized fermentation conditions were as follows:pH 10;NaCl concentration 5%;temperature 25℃;EEEL02 preferred glucose and peptone as carbon and nitrogen sources,respectively.Based on optimal fermentation conditions,EEEL02 induced a significant pH reduction from 10.26 to 5.62 in 5-day incubation test.Acetic acid,propionic acid and CO2(g)were the major acid metabolites of fermentation,suggesting that the pH reduction in bauxite residue may be caused by acid neutralization derived from microbial metabolism.This finding provided the basis of a novel strategy for achieving rapid pH neutralization of bauxite residue.展开更多
On October 13,2020,on the fairway connecting the Polish Baltic ports ofSwinoujscie and Szczecin,an underwater hazardous object of historical origin in the form of the British deep penetration bomb Tallboy dropped duri...On October 13,2020,on the fairway connecting the Polish Baltic ports ofSwinoujscie and Szczecin,an underwater hazardous object of historical origin in the form of the British deep penetration bomb Tallboy dropped during the bombing of the German cruiser"Lützow"in April 1945 was neutralized successfully.It is believed to be the first underwater action concerning this type of bomb,which has previously been neutralized on land in Europe(Germany,one confirmed case).The preparation of the operation,on an unprecedented scale at national,European and global level,took one year and included a series of projects related to clearing the space around the bomb from other identified UXO objects,international consultations,historical analyses,determination of the risk to residents and critical infrastructure in the event of an explosion of the bomb containing approximately 2400 kg of the TORPEX explosive(with an increased force equivalent to almost 3600 kg of TNT).The object was neutralized on spot at the depth of 12 m,near a ferry crossing,by specialists from the 41st Navy EOD Team from the 12th MCM Squadron(8th Coastal Defence Flotilla),using the Low Order Deflagration technique(underwater deflagration method).In the case discussed,there was an accumulation of unfavourable conditions which practically excluded the use of blow-in-situ explosive methods(BIP),as well as the extraction of the object and its transport to a military ground.After a partial deflagration of the explosive,the explosive was detonated(DDT).Estimates indicate that the deflagration level reached between 55 and 60%,which significantly reduced the strength and effects of the underwater explosion.展开更多
Objective Although HIV-1 infection is prevalent in many regions in China, it remains largely unknown on the biological characteristics of dominant circulating isolates. This study was designed to isolate the circulati...Objective Although HIV-1 infection is prevalent in many regions in China, it remains largely unknown on the biological characteristics of dominant circulating isolates. This study was designed to isolate the circulating viral strains from different prevalent regions and to characterize their biological properties and neutralization sensitivity. Methods Primary viruses were isolated from fresh PBMCs using the traditional co-culture method and their capacity of inducing syncytium was tested in MT-2 cells. Meanwhile, their coreceptor usage was determined with two cell lines: Magi and GHOST (3) stably expressing CD4 and the chemokine receptor CCR5 or CXCR4. Furthermore, the sensitivity of these viruses to neutralization by HIV-1-infected patients’ plasma which were highly active to neutralize SF33 strain, was quantified in GHOST cell-based neutralization assay. Results Six primary viral strains were isolated from 4 separated regions. Isolates LTG0213,LTG0214 and XVS032691 induced syncytia in MT-2 cells, and used CXCR4 as coreceptor. Isolates XJN0021, XJN0091, or SHXDC0041 did not induce syncytia, and used CCR5 as coreceptor. Overall neutralization sensitivity differed among four representative strains: HIV-1 XVS032691>LTG0214>XJN0091≈SHXDC0041. Conclusion The neutralization sensitivity of HIV isolates is linked with the phenotype of isolates, in which syncytium-inducing (SI) or CXCR4-tropic (X4) viruses are more easily neutralized than non-syncytium-inducing (NSI) or CCR5-tropic (R5) viruses. The genetic subtypes based on the phylogeny of env sequences are not classical neutralization serotypes.展开更多
An electron cyclotron resonance ion thruster must emit an electron current equivalent to its ion beam current to prevent the thruster system from being electrically charged. This operation is defined as neutralization...An electron cyclotron resonance ion thruster must emit an electron current equivalent to its ion beam current to prevent the thruster system from being electrically charged. This operation is defined as neutralization. The factors which influence neutralization are categorized into the ion beam current parameters, the neutralizer input parameters, and the neutralizer position. To understand the mechanism of neutralization, an experiment and a calculation study on how these factors influence thruster neutralization are presented. The experiment results show that the minimum bias voltage of the neutralizer was -60 V at the ion beam current of 80 mA for the argon propellant, and a critical gas flow rate existed, below which the coupling voltage increased sharply. Based on the experiment, the neutralization was analyzed by means of a onedimensional calculation model. The computation results show that the coupling voltage was influenced by the beam divergence and the negative potential zone near the grids.展开更多
Completely weathered phyllite(CWP)soil is a kind of special soil with high swell potential,while red clay is a special soil with high shrinkage.This means that these two kinds of special soils are usually not suitable...Completely weathered phyllite(CWP)soil is a kind of special soil with high swell potential,while red clay is a special soil with high shrinkage.This means that these two kinds of special soils are usually not suitable for direct use as subgrade fill.To reduce the swell index of the CWP soil and the shrinkage of red clay at the same time,it was proposed to blend the CWP soil with red clay to improve their basic characteristics.A series of swell index tests and dry-wet cycle tests of the blended soils have been carried out at varying blending ratios,compaction coefficients and moisture contents.The test results show that the free swell index of the blended soil decreases with the increase of red clay,moisture content and compaction coefficient,respectively.The fissure density of the blended soil first decreases and then increases with the blending ratio,with the lowest being zero when the blending ratio is ranging from 20%to 40%.Through particle microscopic analysis and elemental composition analysis,it is found that the neutralization effect,the dilution effect of swell minerals,and the partition effect of coarse particles play an important role in restraining expansion and shrinkage deformation of the blended soil.Based on the liquid limit requirement of Chinese Railway Design Code(TB 10001-2016),the optimal blending ratio of red clay has been proposed to be 50%.Compared with the CWP soil,the free load swell index of the blended soil is reduced by 45.0%and the fissure density is reduced by 99.3%compared with that of red clay.Therefore,it is feasible to improve the CWP soil by blending it with red clay at an optimal ratio of 50%by using the neutralization effect of the expansion of CWP and shrinkage of red clay.展开更多
The carbon market auction mechanism is an important policy tool for carbon pricing and a key mechanism that supports carbon emission neutralization,especially for China.A few systematic studies exist on China’s carbo...The carbon market auction mechanism is an important policy tool for carbon pricing and a key mechanism that supports carbon emission neutralization,especially for China.A few systematic studies exist on China’s carbon market auction mechanism.This article focuses on the five auction mechanisms in Chinese pilot emission trading schemes(ETS),reviews the structures and bidding situation of the five-pilot auction mechanism,extracts the similarities,and analyzes their different features,such as auction mode,bidding scale,participants,pricing mode,auction frequency,and so on.This study conducts an in-depth analysis of the carbon allowance auction mechanism in the Guangdong pilot ETS of China,including its development and the evolution of the key elements,its operational effects,and related disputes.Finally,this study puts forward the trend forecast and suggestions for the Chinese allowance auction mechanism,such as the time window of launching national allowance auctions,the most likely auction mode,carbon pricing,and bidding revenue management.Carbon pricing by auction is the most powerful policy tool for addressing carbon emissions reduction and implementing the Glasgow Climate Pact.展开更多
This paper gives a brief account of English consonant cluster's structure and phonetic features from the perspective of the definition and cause of neutralization of English consonants as well as their distinctive...This paper gives a brief account of English consonant cluster's structure and phonetic features from the perspective of the definition and cause of neutralization of English consonants as well as their distinctive features and oppositions.It comes up with the final conclusion that neutralization exists in only thirteen English consonant clusters,among a large number of consonant clusters.展开更多
The increase in anthropogenic carbon dioxide(CO_(2))emissions has exacerbated the deterioration of the global environment,which should be controlled to achieve carbon neutrality.Central to the core goal of achieving c...The increase in anthropogenic carbon dioxide(CO_(2))emissions has exacerbated the deterioration of the global environment,which should be controlled to achieve carbon neutrality.Central to the core goal of achieving carbon neutrality is the utilization of CO_(2) under economic and sustainable conditions.Recently,the strong need for carbon neutrality has led to a proliferation of studies on the direct conversion of CO_(2) into carboxylic acids,which can effectively alleviate CO_(2) emissions and create high-value chemicals.The purpose of this review is to present the application prospects of carboxylic acids and the basic principles of CO_(2) conversion into carboxylic acids through photo-,electric-,and thermal catalysis.Special attention is focused on the regulation strategy of the activity of abundant catalysts at the molecular level,inspiring the preparation of high-performance catalysts.In addition,theoretical calculations,advanced technologies,and numerous typical examples are introduced to elaborate on the corresponding process and influencing factors of catalytic activity.Finally,challenges and prospects are provided for the future development of this field.It is hoped that this review will contribute to a deeper understanding of the conversion of CO_(2) into carboxylic acids and inspire more innovative breakthroughs.展开更多
Neutral beam injection is recognized as one of the most effective means for plasma heating. According to the research plan of the EAST physics experiment, two sets of neutral beam injector(4–8 MW, 10–100 s) were b...Neutral beam injection is recognized as one of the most effective means for plasma heating. According to the research plan of the EAST physics experiment, two sets of neutral beam injector(4–8 MW, 10–100 s) were built and operated in 2014. Neutralization efficiency is one of the important parameters for neutral beam. High neutralization efficiency can not only improve injection power at the same beam energy, but also decrease the power deposited on the heat-load components in the neutral beam injector(NBI). This research explores the power deposition distribution at different neutralization efficiencies on the beamline components of the NBI device. This work has great significance for guiding the operation of EAST-NBI, especially in long pulse and high power operation, which can reduce the risk of thermal damage of the beamline components and extend the working life of the NBI device.展开更多
An E//B neutral particle analyzer(NPA)has been designed and is under development at Sichuan University and Southwestern Institute of Physics.The main purpose of the E//B NPA is to measure the distribution function of ...An E//B neutral particle analyzer(NPA)has been designed and is under development at Sichuan University and Southwestern Institute of Physics.The main purpose of the E//B NPA is to measure the distribution function of fast ions in the HL-2A/3 tokamak.The E//B NPA contains three main units,i.e.the stripping unit,the analyzing unit and the detection unit.A gas stripping chamber was adopted as the stripping unit.The results of the simulations and beam tests for the stripping chamber are presented.Parallel electric and magnetic fields provided by a NdFeB permanent magnet and two parallel electric plates were designed and constructed for the analyzing unit.The calibration of the magnetic and electric fields was performed using a 50 kV electron cyclotron resonance ion source(ECRIS)platform.The detection unit consists of 32lutetium-yttrium oxyorthosilicate(LYSO)detector modules arranged in two rows.The response functions ofα,hydrogen ions(H^(+),H_(2)^(+)and H_(3)^(+))andγfor a detector module were measured with^(241)Am,^(137)Cs and^(152)Eu sources together with the 50 kV ECRIS platform.The overall results indicate that the designed E//B NPA device is capable of measuring the intensity of neutral hydrogen and deuteron atoms with energy higher than 20 keV.展开更多
基金supported by the Natural Science Foundation of China (No. 22268003)。
文摘To avoid carbonate precipitation for CO_(2) electrolysis,developing CO_(2) conversion in an acid electrolyte is viewed as an ultimately challenging technology.In Nature,Xia et al.recently explored a proton-exchange membrane system for reducing CO_(2) to formic acid with a Pb±Pb SO_(4) composite catalyst derived from waste lead-acid batteries based on the lattice carbon activation mechanism.Up to 93%Faradaic efficiency was realized when formic acid was produced by this technology.
文摘Oxidized and acidic mining discharges(pH between 4 and 5)from the Perkoa zinc mine in Burkina Faso consisting of waste rock,crusher rejects and mining tailings were sampled and used for neutralization tests with calcareous dolomite(for six months).The mining discharges and the calcareous dolomite were previously characterized.Columns of 60 cm length and 15 cm diameter were used for the tests.Then,2.62 kg of calcareous dolomite was deposited on the mining discharges in each column.Two liters of deionized water are added every 15 days to each column.Samples were taken at the outlet of the columns at time intervals and then analyzed to assess the evolution of pH,electrical conductivity and contents of PHEs(Potentially Harmful Elements)over time.Following the neutralization tests on solid mining discharges,neutralization tests were also carried out on acid leachates resulting from the oxidation of these same solid mining discharges with calcareous dolomite.The results show that calcareous dolomite is very effective for the neutralization of SR(Sterile Discharges)and the precipitation of PHEs contained therein.It is also effective in buffering acidic effluents from these sterile discharges.As for other mining discharges(crusher rejects and mining residues),although calcareous dolomite is not effective in neutralizing these oxidized and acidic solid discharges,it is however effective in precipitating PHEs such as arsenic and lead.Calcareous dolomite is also effective in buffering acid leachates from these oxidized and acidic mining discharges(crusher rejects and mining tailings).
基金This research was supported by National Science Founda-tion of China (No. 30000123) and Conversation Department of Wildlife Ani-mal & Plants of State Forestry Bureau.
文摘In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda抯 sera was established by using two-fold dilutions of serum and 100 TCID50 of the virus. The 62 sera samples of giant pandas, which were gathered from zoos and reserve region of Sichuan Province, China were detected. The neutralization antibody titer of 1:4 was recognized as the positive criterion, 8 sera samples were detected to be positive, and the positive rate was 12.9%. The titers of neutralizing antibody ranged from 1:8 to 1:32. It was the first comprehensive investigation on neutralization antibodies against CCV in giant panda population in China. The results of study showed that the infection of CCV in giant panda population was universal, which has posed a threat to the health of giant panda. Therefore, it is incumbent on us to study safe and effective vaccines to protect giant panda against CCV infection.
基金supported by grants from National Science and Technology Major Project(2012ZX10004701)
文摘The CD4 binding site(CD4bs) of envelope glycoprotein(Env) is an important conserved target for anti-human immunodeficiency virus type 1(HIV-1) neutralizing antibodies. Neutralizing monoclonal antibodies IgG1 b12(b12) could recognize conformational epitopes that overlap the CD4 bs of Env. Different virus strains, even derived from the same individual, showed distinct neutralization susceptibility to b12. We examined the key amino acid residues affecting b12 neutralization susceptibility using single genome amplification and pseudovirus neutralization assay. Eleven amino acid residues were identified that affect the sensitivity of Env to b12. Through site-directed mutagenesis, an amino acid substitution at position 182 in the V2 region of Env was confirmed to play a key role in regulating the b12 neutralization susceptibility. The introduction of V182 L to a resistant strain enhanced its sensitivity to b12 more than twofold. Correspondingly, the introduction of L182 V to a sensitive strain reduced its sensitivity to b12 more than tenfold. Amino acid substitution at positions 267 and 346 could both enhance the sensitivity to b12 more than twofold. However, no additive effect was observed when the three site mutageneses were introduced into the same strain, and the sensitivity was equivalent to the single V182 L mutation. CRF07_BC is a major circulating recombinant form of HIV-1 prevalent in China. Our data may provide important information for understanding the molecular mechanism regulating the neutralization susceptibility of CRF07_BC viruses to b12 and may be helpful for a vaccine design targeting the CD4 bs epitopes.
文摘This paper analyzed the current situation and development trends of energy consumption and carbon emissions,and the current situation and development trend of coal consumption in China.In the context of recently established carbon peak and carbon neutralization targets,this paper put forward the main problems associated with the green and low-carbon development and utilization of coal.Five key technological innovation directions in mining were proposed,including green coal development,intelligent and efficient mining,low-carbon utilization and conversion of coal,energy conservation and emission reduction,carbon capture,utilization and storage(CCUS).Focusing on the above technological innovation directions,it is suggested to carry out three basic theories,including the theory of green efficient intelligent mining,clean and low-carbon utilization and transformation of coal,and CCUS.Meanwhile,it is proposed to develop 12 key technologies,including green coal mining and ecological environment protection,efficient coal mining and intelligent mine construction,key technologies and equipment for efficient coal processing,underground coal gasification and mining,ultra-high parameter and ultra-supercritical power generation technology,intelligent and flexible coal-fired power generation technology,new power cycle coal-fired power generation technology,the development of coal-based special fuels,coal-based bulk and specialty chemicals,energy conservation and consumption reduction,large-scale and low-cost carbon capture,CO_(2) utilization and storage.Finally,necessary measures from the governmental perspective were also proposed.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (Nos. XDB23030300, XDA1502070901, XDA1502070503)。
文摘To achieve the neutralization control requirements of the radio-frequency(RF)ion microthruster(μRIT)in the‘Taiji-1’satellite mission,we proposed an active neutralization control solution that is based on the carbon nanotube field emission technology.The carbon nanotube field emission neutralizer(CNTN)has the characteristics of light weight,small size,and propellantless,which is especially suitable for the neutralization control tasks of ion microthrusters.The Institute of Mechanics,Chinese Academy of Sciences,in collaboration with Tsinghua University,has successfully developed a CNTN to meet mission requirements.On the ground,the feasibility of cooperation working betweenμRIT and CNTN was fully verified,as well as the simulation and experimental study of neutralization control strategy,which finally passed the engineering assessment test.Since the launch of‘Taiji-1’satellite on 31 August,2019,the RF ion micropropulsion system has successfully completed nearly one hundred test missions in space.The test results indicate that CNTN does not have performance degradation,and the neutralization control strategy is effective.
基金The National Basic Research Program of China under contract No 2006CB101806the National"863"Project of China under con-tract No 2006AA100312
文摘The neutralizing activities of eight monoclonal antibodies (MAbs) against white spot syndrome virus (WSSV) (2D2, 2B2,1D2, 1D5, 1C2, 4A1, 6A4 and 6B4) were analyzed by in vivo experiments. Gills from WSSV-infected shrimp were homogenized and ten-fold serially diluted by PBS, and then incubated with MAbs (hybridoma culture supernatant), respectively. The mixture of WSSV and MAbs were injected into crayfish (Procambarus clarkii). After challenge, the death rates of crayfish were counted to determine the neutralizing activities of MAbs. At the same time, the mixture of myeloma culture supernatant and WSSV or PBS was served as positive or negative control, respectively. The results showed that, at each virus dilution, the mean time to death of the crayfish injected with MAb-treated virus was significantly longer than that in the positive control, though they all showed 100% mortality within 25 d, and meanwhile, few crayfish died in the negative control. Among the eight MAbs, 2D2, 2B2, 1D2 and 1D5, especially the former two, delayed the mortality significantly, and 1 C2, 4A1 and 6A4 delayed the mortality as well but not so efficiently, while MAb 6IM was efficient only when the virus concentration increased. The results indicated that the anti-WSSV MAbs can neutralize WSSV in different virus dilutions.
文摘This study described a regime map for dry neutralization agglomeration. Based on the map, the effects of selected key parameters, such as ingredient composition, operation temperature, agitation speed, and size of Na_2CO_3 particles, were investigated using a laboratory-scale mixer, and properties of the agglomeration product were analyzed, including particle size distribution, Hunter color, and flowability. Torque curves evolving during the process were correlated with the system flowability. Three distinguishable regimes were indicated, dry, wet, and transitional, and the agitation speed was found to have a different influence on the agglomeration process for the three regimes. Furthermore, the influence of temperature on reactive agglomeration significantly differed from that in agglomeration processes in which the binder was non-reactive.
文摘Control of pH neutralization processes is challenging in the chemical process industry because of their inherent strong nonlinearity. In this paper, the model algorithmic control (MAC) strategy is extended to nonlinear processes using Hammerstein model that consists of a static nonlinear polynomial function followed in series by a linear impulse response dynamic element. A new nonlinear Hammerstein MAC algorithm (named NLH-MAC) is presented in detail. The simulation control results of a pH neutralization process show that NLH-MAC gives better control performance than linear MAC and the commonly used industrial nonlinear propotional plus integral plus derivative (PID) controller. Further simulation experiment demonstrates that NLH-MAC not only gives good control response, but also possesses good stability and robustness even with large modeling errors.
基金Projects(41877511,41842020)supported by the National Natural Science Foundation of ChinaProject(502221703)supported by the Innovative Project of Independent Exploration of Central South University,China
文摘Bauxite residue deposit area(BRDA)is a typical abandoned mining wasteland representing extreme hostile environment with increased alkalinity.Microbially-driven neutralization of bauxite residue,based on the microbial acid producing metabolisms,is a novel strategy for achieving rapid pH neutralization and thus improving its environmental outcomes.The hypothesis was that these extreme conditions promote microbial communities which are capable of novel ecologically relevant functions.Several alkaliphilic acid producing bacteria were isolated in this study.One strain was selected for its superior growth pattern and acid metabolism(termed EEEL02).Based on the phylogenetic analysis,this strain was identified as Bacillus thuringiensis.The optimized fermentation conditions were as follows:pH 10;NaCl concentration 5%;temperature 25℃;EEEL02 preferred glucose and peptone as carbon and nitrogen sources,respectively.Based on optimal fermentation conditions,EEEL02 induced a significant pH reduction from 10.26 to 5.62 in 5-day incubation test.Acetic acid,propionic acid and CO2(g)were the major acid metabolites of fermentation,suggesting that the pH reduction in bauxite residue may be caused by acid neutralization derived from microbial metabolism.This finding provided the basis of a novel strategy for achieving rapid pH neutralization of bauxite residue.
文摘On October 13,2020,on the fairway connecting the Polish Baltic ports ofSwinoujscie and Szczecin,an underwater hazardous object of historical origin in the form of the British deep penetration bomb Tallboy dropped during the bombing of the German cruiser"Lützow"in April 1945 was neutralized successfully.It is believed to be the first underwater action concerning this type of bomb,which has previously been neutralized on land in Europe(Germany,one confirmed case).The preparation of the operation,on an unprecedented scale at national,European and global level,took one year and included a series of projects related to clearing the space around the bomb from other identified UXO objects,international consultations,historical analyses,determination of the risk to residents and critical infrastructure in the event of an explosion of the bomb containing approximately 2400 kg of the TORPEX explosive(with an increased force equivalent to almost 3600 kg of TNT).The object was neutralized on spot at the depth of 12 m,near a ferry crossing,by specialists from the 41st Navy EOD Team from the 12th MCM Squadron(8th Coastal Defence Flotilla),using the Low Order Deflagration technique(underwater deflagration method).In the case discussed,there was an accumulation of unfavourable conditions which practically excluded the use of blow-in-situ explosive methods(BIP),as well as the extraction of the object and its transport to a military ground.After a partial deflagration of the explosive,the explosive was detonated(DDT).Estimates indicate that the deflagration level reached between 55 and 60%,which significantly reduced the strength and effects of the underwater explosion.
文摘Objective Although HIV-1 infection is prevalent in many regions in China, it remains largely unknown on the biological characteristics of dominant circulating isolates. This study was designed to isolate the circulating viral strains from different prevalent regions and to characterize their biological properties and neutralization sensitivity. Methods Primary viruses were isolated from fresh PBMCs using the traditional co-culture method and their capacity of inducing syncytium was tested in MT-2 cells. Meanwhile, their coreceptor usage was determined with two cell lines: Magi and GHOST (3) stably expressing CD4 and the chemokine receptor CCR5 or CXCR4. Furthermore, the sensitivity of these viruses to neutralization by HIV-1-infected patients’ plasma which were highly active to neutralize SF33 strain, was quantified in GHOST cell-based neutralization assay. Results Six primary viral strains were isolated from 4 separated regions. Isolates LTG0213,LTG0214 and XVS032691 induced syncytia in MT-2 cells, and used CXCR4 as coreceptor. Isolates XJN0021, XJN0091, or SHXDC0041 did not induce syncytia, and used CCR5 as coreceptor. Overall neutralization sensitivity differed among four representative strains: HIV-1 XVS032691>LTG0214>XJN0091≈SHXDC0041. Conclusion The neutralization sensitivity of HIV isolates is linked with the phenotype of isolates, in which syncytium-inducing (SI) or CXCR4-tropic (X4) viruses are more easily neutralized than non-syncytium-inducing (NSI) or CCR5-tropic (R5) viruses. The genetic subtypes based on the phylogeny of env sequences are not classical neutralization serotypes.
基金support from National Natural Science Foundation of China (Grant No. 11475137)
文摘An electron cyclotron resonance ion thruster must emit an electron current equivalent to its ion beam current to prevent the thruster system from being electrically charged. This operation is defined as neutralization. The factors which influence neutralization are categorized into the ion beam current parameters, the neutralizer input parameters, and the neutralizer position. To understand the mechanism of neutralization, an experiment and a calculation study on how these factors influence thruster neutralization are presented. The experiment results show that the minimum bias voltage of the neutralizer was -60 V at the ion beam current of 80 mA for the argon propellant, and a critical gas flow rate existed, below which the coupling voltage increased sharply. Based on the experiment, the neutralization was analyzed by means of a onedimensional calculation model. The computation results show that the coupling voltage was influenced by the beam divergence and the negative potential zone near the grids.
基金This work is supported by the National Natural Science Foundation of China(Grant Nos.52068027,51668018,51768021).
文摘Completely weathered phyllite(CWP)soil is a kind of special soil with high swell potential,while red clay is a special soil with high shrinkage.This means that these two kinds of special soils are usually not suitable for direct use as subgrade fill.To reduce the swell index of the CWP soil and the shrinkage of red clay at the same time,it was proposed to blend the CWP soil with red clay to improve their basic characteristics.A series of swell index tests and dry-wet cycle tests of the blended soils have been carried out at varying blending ratios,compaction coefficients and moisture contents.The test results show that the free swell index of the blended soil decreases with the increase of red clay,moisture content and compaction coefficient,respectively.The fissure density of the blended soil first decreases and then increases with the blending ratio,with the lowest being zero when the blending ratio is ranging from 20%to 40%.Through particle microscopic analysis and elemental composition analysis,it is found that the neutralization effect,the dilution effect of swell minerals,and the partition effect of coarse particles play an important role in restraining expansion and shrinkage deformation of the blended soil.Based on the liquid limit requirement of Chinese Railway Design Code(TB 10001-2016),the optimal blending ratio of red clay has been proposed to be 50%.Compared with the CWP soil,the free load swell index of the blended soil is reduced by 45.0%and the fissure density is reduced by 99.3%compared with that of red clay.Therefore,it is feasible to improve the CWP soil by blending it with red clay at an optimal ratio of 50%by using the neutralization effect of the expansion of CWP and shrinkage of red clay.
基金supported by Shenzhen Philosophy and Social Sciences Planning in 2021[Grant No.SZ2021A006]the Basic Theoretical Research in the 13th Five Year Plan of Guangdong Philosophy and Social Sciences in 2020[Grant No.GD20 YDXZGL09]and the Characteristic Innovation Projects of Guangdong Universities in China[Grant No.2021WTSCX035].
文摘The carbon market auction mechanism is an important policy tool for carbon pricing and a key mechanism that supports carbon emission neutralization,especially for China.A few systematic studies exist on China’s carbon market auction mechanism.This article focuses on the five auction mechanisms in Chinese pilot emission trading schemes(ETS),reviews the structures and bidding situation of the five-pilot auction mechanism,extracts the similarities,and analyzes their different features,such as auction mode,bidding scale,participants,pricing mode,auction frequency,and so on.This study conducts an in-depth analysis of the carbon allowance auction mechanism in the Guangdong pilot ETS of China,including its development and the evolution of the key elements,its operational effects,and related disputes.Finally,this study puts forward the trend forecast and suggestions for the Chinese allowance auction mechanism,such as the time window of launching national allowance auctions,the most likely auction mode,carbon pricing,and bidding revenue management.Carbon pricing by auction is the most powerful policy tool for addressing carbon emissions reduction and implementing the Glasgow Climate Pact.
文摘This paper gives a brief account of English consonant cluster's structure and phonetic features from the perspective of the definition and cause of neutralization of English consonants as well as their distinctive features and oppositions.It comes up with the final conclusion that neutralization exists in only thirteen English consonant clusters,among a large number of consonant clusters.
基金financial support from the King Abdullah University of Science and Technology(KAUST).
文摘The increase in anthropogenic carbon dioxide(CO_(2))emissions has exacerbated the deterioration of the global environment,which should be controlled to achieve carbon neutrality.Central to the core goal of achieving carbon neutrality is the utilization of CO_(2) under economic and sustainable conditions.Recently,the strong need for carbon neutrality has led to a proliferation of studies on the direct conversion of CO_(2) into carboxylic acids,which can effectively alleviate CO_(2) emissions and create high-value chemicals.The purpose of this review is to present the application prospects of carboxylic acids and the basic principles of CO_(2) conversion into carboxylic acids through photo-,electric-,and thermal catalysis.Special attention is focused on the regulation strategy of the activity of abundant catalysts at the molecular level,inspiring the preparation of high-performance catalysts.In addition,theoretical calculations,advanced technologies,and numerous typical examples are introduced to elaborate on the corresponding process and influencing factors of catalytic activity.Finally,challenges and prospects are provided for the future development of this field.It is hoped that this review will contribute to a deeper understanding of the conversion of CO_(2) into carboxylic acids and inspire more innovative breakthroughs.
基金supported by the International Science and Technology Cooperation Program of China(No.2014DFG61950)National Natural Science Foundation of China(No.11405207)the Foundation of ASIPP(No.DSJJ-15-GC03)
文摘Neutral beam injection is recognized as one of the most effective means for plasma heating. According to the research plan of the EAST physics experiment, two sets of neutral beam injector(4–8 MW, 10–100 s) were built and operated in 2014. Neutralization efficiency is one of the important parameters for neutral beam. High neutralization efficiency can not only improve injection power at the same beam energy, but also decrease the power deposited on the heat-load components in the neutral beam injector(NBI). This research explores the power deposition distribution at different neutralization efficiencies on the beamline components of the NBI device. This work has great significance for guiding the operation of EAST-NBI, especially in long pulse and high power operation, which can reduce the risk of thermal damage of the beamline components and extend the working life of the NBI device.
基金supported by the National Magnetic Confinement Fusion Energy R&D Program of China(No.2018YFE0310200)National Natural Science Foundation of China(Nos.11705242,11805138 and 12175156)the Fundamental Research Funds for the Central Universities in China(Nos.YJ201820 and YJ201954)。
文摘An E//B neutral particle analyzer(NPA)has been designed and is under development at Sichuan University and Southwestern Institute of Physics.The main purpose of the E//B NPA is to measure the distribution function of fast ions in the HL-2A/3 tokamak.The E//B NPA contains three main units,i.e.the stripping unit,the analyzing unit and the detection unit.A gas stripping chamber was adopted as the stripping unit.The results of the simulations and beam tests for the stripping chamber are presented.Parallel electric and magnetic fields provided by a NdFeB permanent magnet and two parallel electric plates were designed and constructed for the analyzing unit.The calibration of the magnetic and electric fields was performed using a 50 kV electron cyclotron resonance ion source(ECRIS)platform.The detection unit consists of 32lutetium-yttrium oxyorthosilicate(LYSO)detector modules arranged in two rows.The response functions ofα,hydrogen ions(H^(+),H_(2)^(+)and H_(3)^(+))andγfor a detector module were measured with^(241)Am,^(137)Cs and^(152)Eu sources together with the 50 kV ECRIS platform.The overall results indicate that the designed E//B NPA device is capable of measuring the intensity of neutral hydrogen and deuteron atoms with energy higher than 20 keV.