Guided by the basic assumptions of cognitive semantics,this paper conducts an analysis on the semantics of Chinese“Lao(old)+N”construction and the semantic relationships between adjective“Lao(old)”and the noun in ...Guided by the basic assumptions of cognitive semantics,this paper conducts an analysis on the semantics of Chinese“Lao(old)+N”construction and the semantic relationships between adjective“Lao(old)”and the noun in order to detect noun selection restrictions of this construction.Under the framework of Taylor’s discussions on adjectives in cognitive grammar,it was found that“Lao(old)+N”Construction could partially testify the feasibility of his assumption.The interpretation of“Lao(old)+N”construction is associated with different senses of“Lao(old)”,as well as the different semantic structures of the nominal constituent.And finally,a plausible explanation on semantic constraints of nouns in this construction is found with the aid of categorization.展开更多
目的构建m asp in/pEFIRES-N表达载体,为深入研究m asp in基因抑制肝癌生长、转移、浸润作用和机制奠定基础。方法PCR扩增m asp in基因全长,将表达载体pEFIRES-N用EcoRⅠ+XbaⅠ进行双酶切后,T4DNA连接酶连接成重组质粒,稳定转染到肝癌...目的构建m asp in/pEFIRES-N表达载体,为深入研究m asp in基因抑制肝癌生长、转移、浸润作用和机制奠定基础。方法PCR扩增m asp in基因全长,将表达载体pEFIRES-N用EcoRⅠ+XbaⅠ进行双酶切后,T4DNA连接酶连接成重组质粒,稳定转染到肝癌高转移细胞株mHCC-97中进行表达,检测稳定转染前后m asp in表达的变化。结果重组质粒在大肠杆菌株JM109内扩增。提纯、纯化后用EcoRⅠ、XbaⅠ酶切鉴定及测序鉴定证明m asp in基因已完整、正确的插入到pEFIRES-N表达载体,并在肝癌MHCC-97细胞中上调了m asp in基因mRNA和蛋白水平表达,抑制了肝癌MHCC-97细胞增殖及浸润。结论成功构建了m asp in/pEFIRES-N表达载体,能在真核细胞中表达。展开更多
文摘Guided by the basic assumptions of cognitive semantics,this paper conducts an analysis on the semantics of Chinese“Lao(old)+N”construction and the semantic relationships between adjective“Lao(old)”and the noun in order to detect noun selection restrictions of this construction.Under the framework of Taylor’s discussions on adjectives in cognitive grammar,it was found that“Lao(old)+N”Construction could partially testify the feasibility of his assumption.The interpretation of“Lao(old)+N”construction is associated with different senses of“Lao(old)”,as well as the different semantic structures of the nominal constituent.And finally,a plausible explanation on semantic constraints of nouns in this construction is found with the aid of categorization.
文摘目的构建m asp in/pEFIRES-N表达载体,为深入研究m asp in基因抑制肝癌生长、转移、浸润作用和机制奠定基础。方法PCR扩增m asp in基因全长,将表达载体pEFIRES-N用EcoRⅠ+XbaⅠ进行双酶切后,T4DNA连接酶连接成重组质粒,稳定转染到肝癌高转移细胞株mHCC-97中进行表达,检测稳定转染前后m asp in表达的变化。结果重组质粒在大肠杆菌株JM109内扩增。提纯、纯化后用EcoRⅠ、XbaⅠ酶切鉴定及测序鉴定证明m asp in基因已完整、正确的插入到pEFIRES-N表达载体,并在肝癌MHCC-97细胞中上调了m asp in基因mRNA和蛋白水平表达,抑制了肝癌MHCC-97细胞增殖及浸润。结论成功构建了m asp in/pEFIRES-N表达载体,能在真核细胞中表达。