Poverty Alleviation through Employment Promotion in Extreme Poverty-stricken Areas in Western China: A Case Study of Targeted Poverty Alleviation through Employment Promotion in Awang Town,Dongchuan District

Poverty alleviation through employment promotion is to promote the poor households to get rid of poverty through promoting employment to increase income. It is the most effective and direct way out of poverty. Dongchuan District, located in the alpine valley area of northeastern Yunnan, is a typical extreme poverty-stricken county in China. Under its jurisdiction, Awang Town is a mountainous township with a wide area of poverty and an extreme poverty. The incidence of poverty in 2015 reached 35.39%. In recent years, through the implementation of various policies to combat poverty, combined with the advantages as a traditional "hometown of migrant labor", focusing on employment- promoting poverty alleviation work, Awang Town has made certain achievements in poverty alleviation work. By the end of 2018, the poverty rate in the town fell to 0.57%, marking a major victory in the town s fight against poverty. Through field investigations, this article summarizes the main practices of Awang Town to carry out employment-promoting targeted poverty alleviation, analyzes the poverty alleviation achievements and successful experience of the town s employment-promoting targeted poverty alleviation work and discusses the promotion and application of this model so as to provide reference Yunnan Province and other poverty-stricken areas to carry out employment-promoting poverty alleviation.

China's Employment Promotion Law Guarantees Right to Employment

China＇s legislature is soliciting comments and suggestions to the draft of Employment Promotion Law as an endeavor to democratize its process of legislation. Up to April 25 the National People＇s Congress had received over 11,000 items of comments and suggestions.

Revolutionary Technologies to Promote Employment

Artificial intelligence will transform the faces of many industry sectors,and also creates a large amount of opportunities.We have witnessed,in the past 10 to 20years,a change within the network industry.I also belive that something big will happen in the next 10 to 20years.We hold great expectations to the

Thoughts on Promoting High-Quality Employment of Students Based on School-Enterprise Cooperation

The effective operation of school-enterprise cooperation is of great strategic significance to promote college students to achieve employment of high-quality.Therefore,this paper will make a detailed discussion on the effect that school-enterprise cooperation accelerates college students to achieve employment in good quality,hoping that it can provide the necessary guidance information to the researchers in this field.

Implementing a More Positive Employment Policy and Promoting the Development of the Chinese Human Rights Cause

Human rights refer to the basic rights to which every person is entitled based on his or her nature and dignlty under certain social and historical circumstances. Simply put, the term means that everyone has the right to live and develop in a free and equal manner.

Genomic Analysis of MicroRNA Promoters and Their Cis-Acting Elements in Soybean

MicroRNAs （miRNAs） are derived from distinct loci in the genome and play crucial roles in RNA-mediated gene silencing mechanisms that regulate cellular processes during development and stress responses of plants. The miRNAs are approximately 21 nucleotides long and code for the complementary strand to a larger genic mRNA. They are often found within the complementary primary transcript （pri-miRNAs）. In the past few years, a growing number of soybean miRNAs have been discovered, however, little is known about the transcriptional regulation of these miRNAs. In this study, promoters and cis-acting elements of soybean miRNAs were analyzed using the genomic data for the first time. A total of 82 miRNAs were located among 122 loci in genome, some were present as double or multiple copies. Five clusters that included ten miRNAs were found in genome, and only one cluster share the same promoter. A total of 191 promoters from 122 loci of the soybean miRNA sequences were found and further analyzed. The results indicated that the conserved soybean miRNA genes had a greater proportion of promoters than that of non-conserved ones, and the distribution of the transcript start sites （TSSs） and TATA-boxes found had different motif styles between conserved and non-conserved miRNA genes. Furthermore, the cis-acting elements 5＇ of the TSSs were analyzed to obtain potential function and spatiotemporal expression pattern of miRNAs. The data obtained here may lead to the identification of specific sequences upstream of pre-miRNAs and the functional annotation of miRNAs in soybean.

Policy variables affecting marginal level of low carbon employment: an empirical study based on provincial data of China

Low Carbon Employment is an inevitable choice for the purpose of "energy saving and emission reduction" and "promoting employment". By Multi variable Linear Backward Regression method, this study presents an empirical analysis of the employment impact of policy variables indexes that involves economic pull, industry upgrading, population development, technical inputs and so on. The paper demonstrates that wide range of factors will affect low carbon employment, that industry upgrading will affect low carbon employment remarkably, that to increase years of people education will notably improve low carbon employment level of secondary vocational-technical labor, and that to raise technical inputs will significantly enhance college students' low carbon employment.

Identification of the development stage-specific factors in mouse fetal liver binding to the human β-globin gene promoter

In order to elucidate the molecular mechanisms of globin gene expression during embryonic development, the nuclear extracts from mouse hematopoietic tissue at different stages of development have been prepared. By using DNase I footprinting and gel mobility shift assays, the binding of protein factors in these extracts to the human βglobin promoter was analyzed. The differences in the binding patterns of protein factors during development were observed. An erythroid-specific and stage-specific nuclear protein in the nuclear extract from d 18 mouse fetal liver was identified, which can bind to the sequence (from -66bp to -90bp) of human β-globin promoter. We therefore speculate that the function of this cis-acting element may be similar to stage selector element (SSE) in chieken βA- promoter.

A stage-specific protein factor binding to a CACCC motif in both human β-globin gene promoter and 5'-HS2region

The DNasel hypersensitive site 2 (HS2) of human β-globin locus control region (LCR) is required fOr the high level expression of human d-globin genes. In the present study, a stage-specific protein factor (LPF-β) was identified in the nuclear extract prepared from mouse fetal liver at d 18 of gestation, which could bind to the HS2 region of humanβ-globin LCRt We also found that the shift band of LPF-βfactor could be competed by humanβ-globin promoter. However, it couldn’t be competed by human E-globin promoter or by human Aβ-globin promoter. Furthermore, our data demonstrated that the binding-sequence of LPF-d factor is 5’CACACCCTA 3’,which is located at the HS2 region ofβ-LCR (from -10845 to -10853 bp) and humanβ-globin promoter (from -92 to -84 bp). We speculated that these regions containing the CACCC box in both the humallβ-globin promoter and HS2 might function as stage selector elements in the regulation of humanβd-globin switching and the LPF-βfactor might be a stage-specific protein factor involved in the regulation of humanβ-globin gene expression.

Act in the Spirit of the 15th Congress of the Party and Strive to Promote the Development of Foreign Trade and Economic Cooperation in Terms of Both Quantity and Quality

China’s foreign trade continued to maintain its development momentum last year througn vigorous promotion of two fundamental changes,spurred by the smooth return of Hong Kong and propelled by the spirit of the 15th Congress of the Communist Party of China. Imports and exports developed steadily。

牡丹花青素苷合成关键基因PsDFR启动子活性分析

[目的]分析牡丹花青素苷合成关键基因PsDFR启动子的顺式作用元件及活性,为进一步研究PsDFR启动子的功能及其在牡丹花色形成中的调控机制奠定基础。[方法]以‘黑花魁’牡丹花瓣中提取的基因组DNA为模板,通过染色体步移法克隆PsDFR的启动子序列。利用生物信息在线软件预测分析启动子序列的顺式作用元件。构建5个不同长度缺失启动子与GUS基因融合的表达载体,并瞬时转化烟草叶片,通过GUS组织化学染色和酶活性检测分析缺失启动子的活性,及其对脱落酸(ABA)、茉莉酸甲酯(MeJA)、光照等不同胁迫处理的响应。[结果]克隆得到PsDFR上游1687 bp的启动子序列。生物信息学分析发现PsDFR启动子含有多个光信号、激素响应、逆境响应及组织特异表达等顺式作用元件,这暗示PsDFR的表达可能受光信号、激素和胁迫等多种信号的共同调节。GUS组织化学染色和酶活性检测表明,随启动子片段的缩短,启动子活性逐渐下降,-1623至-916区段对于启动子活性具有重要作用。MeJA和黑暗处理对各启动子片段活性均有显著抑制作用,恢复光照可促使启动子活性明显回升,而参与ABA响应的核心调控区域位于-443至-76 bp。[结论]PsDFR启动子含有多个光信号、激素响应、逆境响应及组织特异表达等顺式作用元件,其活性受光的正调控以及MeJA的负调控;-1623至-916 bp间的区域对于启动子活性具有重要作用,-443至-76 bp是响应ABA处理的核心区域。该研究为进一步揭示PsDFR应答环境信号参与牡丹花色形成的分子调控机制提供参考。

新时期高职院校学生就业能力提升路径探究

就业是最基本的民生。新时期高职院校学生就业形势日益严峻,文章从高职院校学生就业能力的内涵和特点出发,从个人、学校和社会层面探讨影响学生就业能力的因素,提出高职院校应发展素质教育,提高学生综合素质能力;推进技能教学改革,提高学生专业实践能力;推动育才用人精准匹配,提高学生就业适应能力等,为提升高职院校学生的就业竞争力、发展力,实现学生高质量充分就业提供借鉴。

高职院校深化产教融合的困境与对策研究

深化产教融合是高职院校实现高质量发展的本质要求。文章阐述了产教融合的内涵与目的,剖析了当前新形势下遇到的问题与困境,由此建议政府采取加大政策供给与引导、强化评价导向、激励行业企业办学、增强学校服务行业企业能力等多项举措,为高职院校持续深化产教融合注入活力,提供保障。

公权力介入视域下家庭教育令的适用及完善研究——兼谈《家庭教育促进法》第四十九条之规定

家庭教育经历了从着重权利到强调责任、从纯属私务到公私兼具的属性转变,为公权力介入家庭教育提供了理论基础。家庭教育令是以公权力引导和规范家庭教育的典型令状,其以《家庭教育促进法》为依托,由司法机关于实践中所创设。当前我国家庭教育令的适用在启动上具有明显的附带性和被动性,在文书样式上面临着与家庭教育指导令、督促监护令的混同,同时对拒不履行的法律后果亦存在设定不当。促进家庭教育令制度的完善,可从形式规范化和实质优化双管齐下,前者通过完善制发机制、促进文本的规范统一来实现,对于后者则应理性看待家庭教育主体责任属性,在既有法律体系内寻求妥善执行措施。

听障美术生就业率提升路径研究

就业是民生之本。帮助残疾人实现就业,是巩固脱贫攻坚成果的重要工作。听障美术生作为特殊群体,在教学模式与就业创业上有着极强的特殊性。通过遵循听障美术教育的基本规律,从教育教学、社会、学生三个层面探究适合特殊教育院校人才培养与促进听障美术生就业率提升的有效路径,使他们能够学有所成、增强核心竞争力,更快更好地融入社会,实现自己的人生价值。

数字经济与技能工资差距——基于CFPS的微观证据

随着引领新一轮科技革命和产业升级的数字技术发展,数字经济必将对中国的就业和收入分配格局产生重大影响,使技能工资差距呈现新特征。通过测度2018年中国各行业的数字经济发展水平,借助普通最小二乘法、无条件分位数回归与Oaxaca-Blinder分解等方法,考察数字经济发展对技能工资差距的影响及其作用机制。结果发现:(1)数字经济显著提高了低技能劳动者工资收入,且能缩小低技能群体和高技能群体内部中低收入差距;(2)数字经济能通过就业创造效应和时间投入效应为劳动者提供更多就业机会和就业岗位,提高工作强度,缩小技能工资差距,但职位晋升效应难以充分发挥;(3)数字经济对不同行业部门和不同社会资本群体的技能工资差距的影响存在显著的差异性。据此提出继续推进数字技术发展、积极探索数字经济背景下对高收入的规范与调节路径、完善对低技能群体的劳动权益保障体系、加强学习型社会建设,并以数字化改革推动公共服务高质量发展和实现基本公共服务均等化等政策建议。

大学生就业能力提升导向的导师制实施探析

大学生就业是社会普遍关注的热点问题之一。高校就业导师制度是解决大学生就业困难问题、提升大学生就业质量的重要渠道。分析大学生就业能力困境及问题,结合导师制特征,论述导师制对大学生就业能力提升的作用,探索导师制视域下助力大学生就业能力提升的着力点,强调以大学生就业能力为导向,发挥导师制的积极作用。

延胡索去甲乌药碱合成酶基因CyNCS1克隆及其表达模式分析

【目的】克隆延胡索去甲乌药碱合成酶(norcoclaurine synthase, NCS)基因,分析其在延胡索异喹啉类生物碱合成中的关键作用。【方法】基于转录组数据库,利用逆转录PCR克隆延胡索CyNCS1基因及其启动子区域序列,采用生物信息学方法分析其编码蛋白的理化性质、结构特征及其启动子区域顺式作用元件,同时进行多序列比对和系统进化树分析,确定其进化关系;利用实时荧光定量PCR对其根、茎、叶及发育早、中、晚期块茎的组织表达模式进行分析;对茉莉酸甲酯(MeJA)、脱落酸(ABA)处理0,4,8,12 h的叶片进行表达谱分析,以体积分数75%酒精溶剂处理为对照(CK),确定该基因的表达特征。【结果】CyNCS1基因开放读码框长873 bp,编码291个氨基酸,编码蛋白分子量为33.09 ku,等电点为6.90,具有去甲乌药碱合成酶保守的催化结构域(GDGGVGTV/IL、YKEKF和MIEGGHLDMG),且不含信号肽,预测定位于细胞核中。多序列比对结果显示,CyNCS1与石生黄堇、罂粟的NCS蛋白同源性较高,分别为83.6%和82.1%;系统进化树结果显示,CyNCS1与石生黄堇CsNCS蛋白聚为一支,说明二者亲缘关系较近。该基因启动子区长2 238 bp,包含多种植物激素及低温、热胁迫等环境因子的顺式作用元件。实时荧光定量PCR结果显示,随着发育推进块茎中CyNCS1的表达量显著增高,且受到MeJA和ABA的诱导,MeJA处理8 h时表达量为0 h的3.10倍,ABA处理8 h为0 h的3.16倍;而对照CyNCS1表达量随时间推移无明显变化。【结论】克隆并鉴定了延胡索NCS酶基因CyNCS1,明确了其在发育进程中的表达模式,并确定该基因表达受MeJA和ABA的诱导。