A mechanism to improve early diabetic nephropathy by modulating autophagy-related mechanisms with Yuye Decotion

Objective: To investigate the molecular mechanisms of Yuye Decotion in the treatment of diabetic kidney disease using network pharmacology methods and molecular docking techniques. Methods: Obtain the transcriptome gene expression of diabetic nephropathy through GEO database, and extract genes related to autophagy. Screen the active ingredients and corresponding targets of Yuye Decoction through the TCMSP database, and map the drug prediction targets and disease targets to obtain the autophagy-related Yuye treatment targets for diabetic nephropathy point. Use String database combined with Cytoscape 3.7.2 software to construct the "drug-active ingredient-target" network and protein interaction network of Yuyetang for the treatment of diabetic nephropathy. The target point of liquid soup in the treatment of diabetic nephropathy was analyzed by GO biological process enrichment analysis and KEGG pathway enrichment analysis, and finally used Pymol and other software to analyze the core active components of Yuye Decotion and The core target protein undergoes molecular docking verification. Results: (i)100 eligible diabetic nephropathy and autophagy related genes were screened, and the potential targets of Yuye Decoction were 1,428. The acquired genes related to diabetic nephropathy and autophagy were mapped to potential targets of Yuye Decoction, and 22 therapeutic targets were obtained. GO biological process enrichment analysis and KEGG pathway enrichment analysis found that the pathways related to autophagy in the treatment of diabetic nephropathy by Yuye Decoction may include mTOR signaling pathway, phospholipase D signaling pathway, insulin resistance, EGFR tyrosine kinase inhibitor resistance, Apoptosis, PI3K /Akt signaling pathway, NF-κB signaling pathway, etc. (ii)The protein interaction network shows that VEGFA, ERBB2, GASP3, MAPK8, MYC, CDKN1A, EGFR, BCL2L1 may be the key targets of Yuye Decoction in the treatment of diabetic nephropathy. Molecular docking realizes the binding of 4 core active ingredients to 8 core target proteins. Conclusions: The research results show that Yuye Decoction treats diabetic nephropathy through multi-component, multi-target, and multi-pathway action, and provides new theoretical basis for the study of pharmacological effects and clinical application of Yuye Decoction in the treatment of diabetic nephropathy in autophagy-related aspects.

Clinical Study on Rexiao decotion for asthma of heat wheezing type at acute stage

Objective:To study the safety,efficacy and mechanism of Wang Yulin self-made recipe Rexiao decoction in the treatment of acute bronchial asthma(BA).Methods:80 patients with acute attack BA were randomly divided into western medicine group(n=40)and traditional Chinese and western medicine group(n=40).The control group was treated with routine western medicine:salmeterol was inhaled twice a day,combined with oxygen therapy and inducement therapy.The Chinese and Western medical group was treated with Rexiao decoction in the western medical group.The course of treatment is 15 days.Their syndrome score,blood gas analysis(PaC02,Pa02)and induced sputum IL-5,EOS,IL-1 were observed.3.Pulmonary function(record of kidney,liver function,blood routine and adverse reactions in PEF%Pred,FEV1%Pred),group).Results:the total effective rate was 85%in western medicine group and 92.5%in traditional Chinese and western medicine group(P<0.01).After treatment,the score of TCM syndromes in the Chinese and western medicine group was better than that in the western medicine group(P<0.05).After treatment,the pulmonary function(FEV1%Pred)of the traditional Chinese and western medicine group was better than that of the western medicine group,and the PEF%Pred of the Chinese and western medicine group was better than that of the western medicine group.Analysis of Blood Gas in traditional Chinese and Western Medicine Group after treatment(PaO2)was significantly higher than that in the control group(P<0.05).There was no significant difference in the improvement of blood gas analysis(PaCO2)between the western medicine group and the traditional Chinese and western medicine group(P>0.05).The decrease of IL-13,EOS and IL-5 in sputum of traditional Chinese and western medicine group was more than that of western medicine group(P<0.01).There was no significant difference in liver function(AST,ALT),renal function(Cr,Urea),blood routine(WBC,RBC,PLT)before and after treatment in traditional Chinese and western medicine group(P>0.05),but no obvious adverse reactions were found.Conclusions:heat Asthma decoction is safe and effective in the treatment of hot asthma syndrome in acute attack.The target and inflammatory factors,eosinophils are likely to decrease,and the effect of airway inflammation and high reaction are decreased.

Radar Echo and Lightning Characteristics Analysis on A Strong Thunderstorm Weather in Fuxin

Based on the radar data and lightning position indicator data of strong thunderstorm weather which happened in Fuxin on July 8,2007,the relationship between the lightning activity and the radar echo was analyzed.The results showed that Fuxin area located in the cross position of T-shaped trough and was affected by the cold air which continuously glided down.The corresponding warm front on the ground advanced southward and arrived here.It was the weather background of this thunderstorm weather.The position variation of lightning occurrence was closely related to the strong echo movement of squall line,and the velocity echo clearly reflected and predicted the movement tendency of the radar echo.

Economic Vulnerability of Mining City——A Case Study of Fuxin City,Liaoning Province,China

The economic system of mining city is of typical vulnerability characteristics that can be manifested by its high economic sensitivity and lack of response capacity to the gradual depletion of regional mineral resources. Taking Fuxin City of Liaoning Province as a case, this paper established an economic vulnerability assessment method integrating BP neural network with vulnerability index, then carried out an economic vulnerability assessment of Fuxin during 1989-2006. The results indicate that: 1) Affected by the gradual depletion of regional mineral resources, the economic development of Fuxin had kept high economic sensitivity from 1995 to 2001, and the response capacity to cope with and adapt to the impacts of the perturbation of mineral resources was weak and relatively lag. The evolution of economic vulnerability can be divided into three stages: in 1989-1994, the economic vulnerability of Fuxin City decreased slowly; in 1995-2001, the beginning stage of economic transformation, the economic vulnerability of Fuxin City went up rapidly; in 2002-2006, the economic vulnerability of Fuxin City descended and showed a trend to be stable. 2) The influence of economic sensitivity on Fuxin′s economic vulnerability is more evident than that of response capacity. 3) The decreasing supply of mineral resources and the simple industrial structure are main factors leading to the economic sensitivity of Fuxin. 4) The improvement of economic response capacity of Fuxin has typical characteristics of input-driven growth, and external assistance is of great importance to the rapid improvement of economic response capacity of Fuxin. And 5) the change from the simple industrial structure to diversified one of Fuxin is still unaccomplished, and the contribution of non-coal-based industry to local economic development is relatively limited.

Study on Potential and Countermeasures of Artificial Precipitation Enhancement in Fuxin Region

To exploit cloud water resources to increase precipitation on the ground effectively,according to the principles of artificial precipitation enhancement,based on historical data of weather and precipitation from Fuxin National Basic Meteorological Stations from 1981 to 2010,the distribution characteristics of cloud water resources,potential and countermeasures of artificial precipitation enhancement in Fuxin region of Liaoning Province were studied. The results show that the annual average quantity of cloud water resources in the air over Fuxin region over the past 30 years was 285. 6 × 10~8m^3,but the annual average precipitation was only 471.8 mm( amounting to 47.2 ×10~8m^3 of water resources),accounting for only16. 5% of total quantity of cloud water resources in the air; 12 times of artificial precipitation enhancement operation experiments were carried out by using the weather models and technical indicators suitable for artificial precipitation enhancement in Fuxin region,and the artificial precipitation efficiency was 18. 3% on average,5. 2% higher than before,and the annual average precipitation could increase by 2. 5 × 10~8m^3 approximately. All of these have great significance for implementing artificial precipitation enhancement operation scientifically and effectively,maximizing the exploitation of cloud water resources in the air,increasing total quantity of water resources,and mitigating drought in Fuxin region.

Effect of Heat Resources Characteristic Change on Agriculture in Fuxin Area of Liaoning

The analysis on the trend change of heat resources serviced for the industrial structure adjustment of agriculture and the increase of agricultural economical income.The result showed that increase trend of heat resources in Fuxin region was obvious.≥0 ℃ first day trend advanced 12 d,and the interval days trend increased 17 d.Moreover,the accumulated temperature trend increased 396 ℃.≥10 ℃ first day trend advanced 7 d,and the final day trend delayed 9 d.Moreover,the interval days increased 15 d,and the accumulated temperature trend increased 350 ℃.The latest frost date trend advanced 10 d,and the first frost date trend delayed 12 d.Moreover,the frost-free period trend prolonged 23 d.On one hand,the increase of heat resources favored the adjustment of crops variety structure,the development of agricultural facilities,the change of agricultural measures,the improvement of multiple cropping indexes and the increase of agricultural economical income.On the other hand,it made that the agricultural pests shifted northward by passing the winter boundary,and the insect source radix increased.In addition,the heat resources increase would also cause that the soil potential evapotranspiration increased,and the precipitation utilization ratio decreased.Then,the agricultural production environment worsened,and the dry land agriculture,irrigated agriculture were threatened.It could be said that the advantages and disadvantages of climate warming and heat resources increase on the agricultural production both existed.

Terrestrial records of the early Albian Ocean Anoxic Event:Evidence from the Fuxin lacustrine basin,NE China

The early Albian Oceanic Anoxic Event 1 b(OAE 1 b) is well documented in the Tethys, Pacific and North Atlantic, but few studies have evaluated whether or not terrestrial records of OAE 1 b exist. In order to identify terrestrial records of the early Albian OAE 1 b and to infer possible driving mechanisms, an integrated multi-proxy study from the late Aptian to Albian in the Fuxin lacustrine basin was conducted,including thick, organic-rich black mudstones, total organic carbon(TOC), organic carbon isotopes(d13 Corg), mercury concentration(Hg) and results from pyrolysis analyses(S2, Tmaxand HI). Results show three distinct short-term negative d13 Corgexcursions corresponding with relatively high TOC values,which could be counterparts of the Kilian, Paquier and Leenhardt sub-events of the early Albian OAE1 b. Atmospheric CO2 concentration(p CO2) recovered from C3 plant d13 Corgcompositions indicates an increasing trend in Unit C during the early Albian, and there are three short-term increases of p CO2 corresponding to the three sub-events of OAE 1 b at this time interval. We infer that a trend of increasing p CO2 during the Kilian sub-event in the study area is closely related to volcanism. Continental weathering calculated using chemical weathering indices(CIA, WIP and MIA(O)) show an increasing trend during the OAE 1 b interval, likely resulting from warmer and more humid conditions. Mixed sources of terrestrial plants and lacustrine plankton demonstrated by pyrolysis analyses(HI vs. Tmaxand S2 vs. TOC), indicate a terrestrial contribution to the organic-rich sediments of the Kilian, Paquier and Leenhardt sub-events of OAE 1 b. We suggest that a CO2-forced greenhouse effect during the early Albian might have triggered the relatively warm and humid palaeoclimatic conditions, and intensified chemical weathering that combined to create high nutrient and organic matter levels that were flushed into lakes contributing to eutrophication and anoxia in lacustrine and in contemporaneous oceanic systems.

The stress state of geological structure and mining dynamic disaster in Fuxin basin

Further evidences show that most mining dynamic disasters are mainly oc- curred nearby NNE and near SN geological structures.In-situ stress measurement in Fuxin basin shows that the orientation of major compressed stress is near EW.At this stress field,geological structures with deferent strike have deferent stress state and dis- place mode.NNE and near SN geological structures are compressed to thrust and come into being high stress zone.NWW and NEE geological structures are tensile to separate and not prone to being low stress zone.NW structure is intervenient of them.So NEE and near SN structures are easy to occurre mining dynamic disasters and NWW and NEE structures is 'safety' comparatively.The mining dynamic disaster is controlled by stress state of geologic structure,which is determined by its strike.

Research on Thunderstorm Forecasting in Fuxin, China Based on Physical Diagnostic Parameters

Thunderstorms are very spectacular super-long-range discharge processes in the atmosphere, which can cause tremendous damage in an instant, often leading to casualties, resulting in damage to buildings, power supply systems, communication equipment and forest fires, causing major economic losses. In order to successfully predict thunderstorms, and many economic losses can be avoided. Using the observation data of two county stations in Yimeng County and Zhangwu County from June to August 2009-2015, 40 typical thunderstorm weather processes were selected, and 15 convective parameters related to thunderstorm activities were calculated. After statistical analysis, there are seven convective parameters with significant correlation with thunderstorm activity: convective affective potential energy (CAPE), 850 hPa specific humidity, 700 hPa specific humidity, 850 hPa false equivalent temperature, maximum rising speed, strong weather threat index (SWEAT) and zero degree height (ZH), and the correlation is greater than 0.3. We determined the forecast threshold of the above forecasting factors, calculated the fitting rate and conducted a test report. We used the pup product to establish a short-term proximity indicator for thunderstorm warning. Three products with combined reflectivity, vertical integrated liquid water content and echo top height were selected as warning indicators for thunderstorms. The above research results were used to forecast the thunderstorm weather from June to August in the year of 2015 and 2016. The forecast accuracy rate is more than 85%. In summary, the above methods have reference value and indicative significance for the forecast and warning of thunderstorm weather in Fuxin City, China.

Numerical simulation of deep-level rockburst in Fuxin coalfield

On the basis of ANSYS finite element model(FEM) software, the deep-level rockburst in Fuxin coalfield was simulated numerically. Based on Haizhou Mine and Wulong Mine as two typical deep-level rockburst examples in Fuxin coalfield, the rules and characteristics of the deep-level rockburst were analyzed. And the models were es- tablished. For Haizhou mine, the relationship between mining distance and rockburst was presented when 100, 300, 600 m were mined in 3313 working face. When 300 m were mined, the rockburst began to emerge. When 600 m were mined, the rockburst was the most possible to happen and the compression stress of the working face reached to the maximum value. The effect of tectonic stress on synclinal axis is also a key factor to rockburst occurrence. This was verified by the rockburst happened when 496 m were mined. For Wulong mine, based on the 311 working face as an example, the contours of Y stress in the roof and floor were obtained when the mining distance were 100, 200, 300 and 400 m. When 100 and 400 m were mined, the high stress con- centration regions occurred in the front of working face. This shows the rockburst is easy to happen. It is confirmed by the rockburst when 91m were mined in 311 working plane. The above indicates that the numerical simulation has instructive rule to study the deep-level rockburst in Fuxin coalfield.

Analysis of Action Mechanism of Fuxin Decoction on Heart Failure Based on Network Pharmacology

[Objectives]The purpose was to analyze and predict the mechanism of Fuxin decoction in the treatment of heart failure based on network pharmacology.[Methods]Relevant tools and methods of network pharmacology were used to obtain the active ingredients and action targets of Fuxin decoction and action targets of heart failure,find out the key targets and core clusters of Fuxin decoction on heart failure,and analyze related signal pathways,to explore possible molecular mechanisms.[Results]A total of 53 active ingredients and 224 action targets of Fuxin decoction,1010 heart failure-related target genes,and 94 drug-disease directly acting targets were obtained.From the PPI network constructed,225 key targets and a core cluster composed of 56 nodes and 297 interactions were screened out.Association with tumors,glutamate synapse and other related pathways and related genes such as adenylate cyclase and MAPK were known.[Conclusions]The mechanism of Fuxin decoction in the treatment of heart failure is related toβARs-G protein-adenylate cyclase,PI3K-Akt signaling pathway and HSP90,MAPK and other proteins.

Impact of Environmental Factors on Respiratory Diseases in Fuxin City and Its Prediction

Based on the daily data of visits for respiratory diseases in two grade A hospitals as well as meteorological factors and air pollution in Fuxin City from December 1, 2020 to November 31, 2021, PCA and RBF neural network were used to study the effects of meteorological factors and air pollution on respiratory diseases and predict them. The results showed that the number of daily visits was the largest in winter(accounting for 62.5%), followed by spring(15.2%), and it was the smallest in autumn(only 6.9%). The correlation between the number of daily visits and meteorological factors was higher than that of air pollution factors, and the correlation with temperature and ozone was the highest. The response coefficient of daily visits to each factor increased first and then decreased within 9 d, and the peak was 4-5 d behind. RBF and PCA-RBF neural network models were established to predict the number of daily visits, and the accuracy was 86.3% and 95.2%, respectively.

Relationship between Precipitation Characteristics and Solar Activity in Fuxin from 1951 to 2019

Based on the monthly precipitation data in Fuxin from 1951 to 2019,the M-K and Pettitt mutation test,Morlet wavelet analysis,CWT,XWT and WTC methods were used to study the correlation between precipitation and sunspots in Fuxin in the past 69 years.The results show that from 1951 to 2019,only the spring precipitation in Fuxin showed an upward trend,and annual precipitation,the precipitation in the other three seasons and the annual number of sunspots all showed a relatively obvious downward trend.The first main cycles of annual precipitation,the annual number of sunspots,spring,summer,autumn and winter precipitation were quasi-28,quasi-16,quasi-51,quasi-51,quasi-27 and quasi-11 years,respectively.Annual precipitation,the annual number of sunspots,spring,summer,autumn and winter precipitation changed suddenly in 1980,2005,2005,1980,1992 and 1980,respectively.Both annual precipitation and seasonal precipitation had a relatively obvious resonance cycle of 5-15 years with sunspots,indicating that there was a good correlation between the annual number of sunspots and annual precipitation on an interannual scale.

基于化学成分与药理作用评价当归-黄芪药对配方颗粒汤剂与传统汤剂的差异

目的评价当归-黄芪药对配方颗粒汤剂(DGD)与传统汤剂在化学成分和药理效应方面的差异,为合理应用该配方颗粒提供参考。方法以原料来源批次统一和不统一2种对比方式,设不同样品组,采用高效液相色谱(HPLC)法建立特征图谱,从特征图谱相似度、成分种类、指标成分含量、共有峰面积等角度对化学成分进行评价;采用失血性血虚模型小鼠评价药效。结果①DGD特征图谱与传统汤剂相似度较高(相似度>0.87);②共有色谱峰数目不一致,传统汤剂-自购饮片和传统汤剂-A厂饮片共有色谱峰各12个,A厂DGD共有色谱峰15个,B厂DGD共有色谱峰10个;③A厂DGD中阿魏酸、毛蕊异黄酮苷含量高于传统汤剂(P<0.05);B厂DGD与传统汤剂阿魏酸含量差异无统计学意义,但毛蕊异黄酮苷含量较传统汤剂低(P<0.05);④DGD共有峰面积总和与传统汤剂相比,自购传统汤剂、A厂传统汤剂及A厂配方颗粒、B厂配方颗粒中各成分含量相对比值分别为1.00、0.96、2.14、0.60;⑤DGD及传统汤剂均能明显促进失血性贫血模型小鼠血红蛋白(Hb)、红细胞(RBC)恢复(P<0.01);与模型对照组比较,除B厂DGD组外均差异有统计学意义(P<0.05);A厂DGD与传统汤剂差异无统计学意义,B厂DGD与传统汤剂差异有统计学意义(P<0.01)。结论无论原料来源批次是否一致,DGD与传统汤剂在化学成分上均存在一定差异;在药理作用上,来源于同一批次饮片制备的DGD与传统汤剂对改善失血性贫血药效相当,来源于不同批次饮片制备的DGD与传统汤剂在药效上存在一定差异;不同来源批次的饮片和不同制备工艺造成不同厂家配方颗粒存在质量差异,说明国家统一配方颗粒质量标准及制定相关过程规范具有必要性与紧迫性。

排石汤对肾结石模型大鼠保护作用及机制研究

[目的]观察排石汤对肾结石模型大鼠肾功能的保护作用,并初步探讨其机制。[方法]采用1%乙二醇和2%氯化铵溶液灌胃建立肾结石大鼠模型,将40只健康雄性SD大鼠随机分为正常组、模型组、枸橼酸钾组和排石汤低、高剂量组,每组8只。给药4周,检测24 h尿量、肾脏系数、血清尿素氮(BUN)、肌酐(Scr)等生化指标,苏木精-伊红(HE)染色检测肾损伤,酶联免疫吸附(ELISA)法检测炎症因子肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)及白细胞介素-1β(IL-1β),免疫组化法测定转化生长因子-β1(TGF-β1),Western blot法测定丝裂原活化蛋白激酶(p38MAPK)。[结果]与模型组比较,排石汤高、低剂量组24 h尿量明显增加(P<0.01),肾脏系数明显降低(P<0.01),肾损伤程度较轻。与模型组比较,排石汤高、低剂量组BUN和Scr水平明显降低(P<0.01)。与模型组相比,排石汤高、低剂量组TNF-α、IL-6和IL-1β指标显著降低(P<0.01)。与模型组比较,排石汤高、低剂量组TGF-β1/p38 MAPK水平显著低于模型组(P<0.01)。[结论]排石汤能抑制草酸钙结石形成,改善大鼠肾脏功能,降低血中炎症细胞因子水平,抑制TGF-β1/p38 MAPK信号通路,进而发挥肾脏保护作用。

化瘀祛湿方对miR⁃21靶向Skp2介导早期膝骨关节炎软骨细胞自噬的影响

目的:研究miR‑21‑5p介导Skp2对膝OA软骨细胞自噬影响,探讨化瘀祛湿方对膝OA软骨细胞的保护作用。方法:(1)取全膝关节置换术的人体膝OA软骨组织,采用FISH单染试验检测miR‑21‑5p表达和定位,免疫荧光检测Skp2表达,TUNEL染色试验检测膝OA细胞凋亡,Western blot法检测Bax、Bcl‑2、Beclin1、CARM1、LC3Ⅱ/Ⅰ和Skp2相对表达量;(2)取SD大鼠36只,按随机数字法分为正常对照组、假手术组、模型组、模型+空载组、模型+miR‑21‑5p干扰组、模型+化瘀祛湿方组6组,每组6只;采用石蜡切片阿利新蓝染色观察软骨形态结构,qPCR、Western blot法检测各组软骨组织Beclin1、CARM1、LC3Ⅱ/Ⅰ、Skp2相对表达量,免疫组化检测Skp2的IOD/Area值。结果:FISH检测显示miR‑21‑5p在人体膝OA软骨组织中高表达,免疫荧光检测表明Skp2在人体膝OA软骨组织中低表达;TUNEL检测发现,人体膝OA软骨组织凋亡显著增加;Western blot法检测显示,与正常对照组相比,人体膝OA软骨组织中Bax、CARM1相对表达量较正常组显著升高,而Bcl‑2、LC3Ⅱ/Ⅰ、Skp2相对表达量明显降低,Beclin1相对表达量稍降低(P<0.05)。动物实验显示,采用miR‑21‑5p干扰处理后,Beclin1、CARM1、LC3Ⅱ/Ⅰ表达显著下降,Skp2稍降低;免疫组化检测发现Skp2 IOD/Area值与正常对照组相比,模型组IOD/Area值显著降低(P<0.05);与模型组相比,模型+化瘀祛湿方组IOD/Area值显著升高(P<0.05);石蜡切片阿利新蓝染色显示与假手术照组相比,模型+空载组、模型+miR‑21‑5p干扰组蓝色变浅,但模型组蓝色变深,模型+化瘀祛湿组蓝色较为明显。结论:miR‑21‑5p可负向调控Skp2,介导膝OA软骨细胞自噬,加速OA发展;化瘀祛湿方可能通过抑制miR‑21‑5p,提高Skp2表达,起到延缓膝OA退变的作用。

复心合剂治疗阳虚水泛型心肌梗死后心力衰竭的前瞻性临床研究

目的探讨复心合剂治疗阳虚水泛型心肌梗死后心衰患者临床疗效及对血清心肌损伤、心室重构相关指标的影响。方法将92例阳虚水泛型心肌梗死后心衰患者随机分为观察组与对照组各46例。对照组给予西医基础治疗,观察组在对照组治疗基础上加服复心合剂,治疗前后评估2组患者的中医证候积分,超声心动图指标[左心室射血分数(LVEF)、左心室Tei指数、每搏输出量(SV)],血清心肌损伤指标[血清N末端B型利钠肽原(NT-ProBNP)水平、血清高敏心肌肌钙蛋白I(hs-cTnI)水平、肌酸激酶同工酶(CK-MB)水平],血清心室重构指标[缺氧诱导因子1α(HIF-1α)、可溶性生长刺激表达基因2蛋白(sST2)、血管紧张素Ⅱ(AngⅡ)],明尼苏达州心功能不全生命质量量表(MLHFQ)变化情况,治疗后随访1年观察2组患者主要不良心血管事件发生情况。结果治疗后,2组中医证候积分均显著降低(P<0.01),观察组优于对照组(P<0.05,P<0.01)观察组中医临床总有效率优于对照组(P<0.05);2组患者血清超声心动图指标,心肌损伤、心室重构指标及MLHFQ评分均显著降低(P<0.01),观察组优于对照组(P<0.01)。随访期间,观察组治疗后全因死亡率、恶性心律失常率、急性心力衰竭率均显著低于对照组(P<0.05,P<0.01)。结论复心合剂治疗阳虚水泛型心肌梗死后心衰患者临床疗效显著,疗效机制可能与其减轻心肌损伤、抑制心室重构有关。

清热养阴法阻断社区获得性肺炎转化重症的回顾性分析

目的总结非重症社区获得性肺炎(CAP)院内发生重症肺炎(SP)的相关因素,同时探究早期运用清热养阴方对减少SP发生及相关预后的影响。方法采取回顾性研究的方法,收集符合标准的2018年6月至2023年3月间江苏省中医院急诊科病房收治的CAP病例资料,分析院内SP的发生情况,以及影响SP发生的因素,并根据住院前5 d完整运用清热养阴方中药参与治疗,定义为中药组,其余为对照组,分析中药治疗对减少SP发生与临床疗效的影响。结果共纳入研究病例1235例,其中院内14 d内发生SP 79例,其中,年龄≥65(OR=3.673)、中药治疗(OR=2.683)、低氧血症(OR=5.788)、白细胞(OR=1.592)、降钙素原(OR=0.491)、胆碱酯酶(OR=0.998)为影响中低危CAP院内发生SP的因素;中药组680例,对照组555例,在倾向性匹配后,各497例纳入分析,中药组院内SP发生率低于对照组(P=0.041),临床有效率优于对照组(P=0.025)。结论中低危CAP病患中,年龄≥65岁,PSI评分、白细胞计数、降钙素原高,胆碱酯酶低,合并低氧血症可增加院内发生SP的风险,同时清热养阴方早期运用可减少重症的发生,并对预后有积极意义。

基于网络药理学和分子对接探讨黄芪补肾活血汤治疗乳腺癌的作用机制

目的:基于网络药理学和分子对接预测黄芪补肾活血汤治疗乳腺癌的作用机制。方法:利用中药系统药理学数据库和分析平台(traditional Chinese medicines systems pharmacology platform,TCMSP)、BATM-TCM数据库等筛选黄芪补肾活血汤主要活性成分以及相关靶点,通过Gene Cards数据库搜索乳腺癌相关基因并进行筛选,将黄芪补肾活血汤靶点与乳腺癌基因进行映射,利用Cytoscape 3.8.2软件构建中药-成分-靶点可视化网络,绘制蛋白互作网络及进行基因本体(gene ontology,GO)、京都基因和基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集分析,筛选出核心基因。将黄芪补肾活血汤复方成分与关键靶点通过Swiss Dock网站进行分子对接验证。结果:黄芪补肾活血汤有效成分136个,潜在治疗靶点828个,其中76个是黄芪补肾活血汤治疗乳腺癌的潜在靶点。在蛋白互作网络中发现TP53、VEGFA、MAPK8、JUN、EGF等可能是黄芪补肾活血汤治疗乳腺癌的关键靶点。通过GO、KEGG富集分析发现作用机制涉及的通路包括PI3K-Akt信号通路、肿瘤中的microRNAs、RNA聚合酶Ⅱ转录因子复合物肌膜、转录因子结合、G蛋白偶联胺受体活性、血红素结合、蛋白质结构域特异性结合、核受体活性、癌症中的转录调控异常、血管内皮生长因子信号通路等。通过分子对接验证显示,化合物槲皮素、山柰酚、β-谷甾醇、木犀草素、植物甾醇与关键靶点PGR、ADRB2、NR3C2、PREE1、SCN5A具有较好的结合能力。结论:通过网络药理学构建“药物-成分-靶点-信号通路”网络及分子对接技术验证,发现黄芪补肾活血汤治疗乳腺癌主要是通过抗癌、抑制雌激素受体的表达、调节免疫等多通路、多靶点实现的,为临床治疗乳腺癌提供了更多的治疗方法,给临床用药提供了更多的选择。

当归拈痛汤治疗急性痛风性关节炎的作用机制研究进展

急性痛风性关节炎(AGA)是最常见的痛风急性期表现形式之一,发作时可引起关节剧痛,严重影响患者的生活质量。而当归拈痛汤具有清热除湿、缓急止痛之功效,是治疗AGA的常用方剂,尤擅治疗湿热蕴结型AGA。当归拈痛汤由羌活、茵陈、防风、苍术、当归身、知母、猪苓、泽泻、升麻等药物组成,诸药合用,共奏清热利湿、疏风止痛之效。其具有抑制炎症反应、促进尿酸排泄、改善关节病变、抑制氧化应激等作用,但在AGA治疗中的具体作用机制仍需大量的基础及临床研究验证。