Environmental DNA(eDNA)has been used as an important tool for fish diversity analysis,which can greatly solve the problems in traditional survey methodology.However,little work has been done on the actual monitoring a...Environmental DNA(eDNA)has been used as an important tool for fish diversity analysis,which can greatly solve the problems in traditional survey methodology.However,little work has been done on the actual monitoring accuracy of eDNA.In this study,we analyzed the current status of fish resources in Erhai Lake in Yunnan,SW China,by dividing the lake into three sectors according to habitat differences,and compared the results of eDNA and traditional capture methods to investigate the shortcomings of the current analysis of eDNA results.A total of 27 fish species were detected by eDNA and traditional capture methods,including 20 and 19 fish species,respectively,and additional differences in fish composition between the two methods.The alpha diversity showed higher fish abundance and lower fish diversity by eDNA method compared to the traditional capture method,demonstrating that eDNA was not superior for use in fish diversity analysis.Fish community similarity analysis showed that community differences were generally significant for eDNA(P<0.05).RDA analysis indicated that environmental factors did not significantly affect fish communities monitored by the eDNA method.However,water temperature,aquatic plants,and water depth had significant(P<0.05)effects on fish communities in the traditional capture method,suggesting that eDNA results are insensitive to the effects of environmental factors.Our results illustrate the effectiveness of eDNA in fish identification and the issues in quantification compared to traditional capture methods.Therefore,combining eDNA with traditional methods is a more effective method for analyzing eDNA metabarcoding,following which the protocols of both quantitative methods can be designed to explore the regularity of eDNA quantification.展开更多
The fish cage design requires accurate predictions of long-term extreme loads and responses.Compared with the time-consuming full long-term analysis method integrating all the probability distribution of the short-ter...The fish cage design requires accurate predictions of long-term extreme loads and responses.Compared with the time-consuming full long-term analysis method integrating all the probability distribution of the short-term extremes,the environmental contour method gains much attention in predicting the long-term extreme values due to the less computational effort.This paper investigates the long-term extreme response of a fish cage using the environmental contour method.The fish cage is numerically simulated based on the lumped-mass method and the curved beam theory.Based on the one-dimensional(1D)and two-dimensional(2D)environmental contour,the extreme responses,including the surge and heave motions,mooring force,and vertical bending of the floater,are predicted for different return periods and compared with the full long-term analysis results.Results indicate that the 1D method greatly underestimates the extreme values.The 2D environmental contour method with a higher percentile level,namely90%,provides reasonable estimations and seems to be suitable for the long-term value analysis.Sensitivity studies show that the mooring arrangement and the bending stiffness have great effects on the bending moment and the mooring force and the mooring line pre-tension has minor effects on the fish cage response.展开更多
"Give a man a fish and you feed him for a day;teach a man to fish and you feed him for a lifetime".This proverb now becomes the slogan of China's international aid.This paper is based on my previous empi..."Give a man a fish and you feed him for a day;teach a man to fish and you feed him for a lifetime".This proverb now becomes the slogan of China's international aid.This paper is based on my previous empirical studies on China's"donor logic"of aid.Rather than looking at the motives of Chinese aid and how it is challenging the Western ideas,this paper focuses on policy discourse and tries to seek the recent trends and knowledge transformations of China's international cooperation of education.展开更多
近年来,FISH(Fluorescence in Situ Hybridization,荧光原位杂交)法作为一种应用分子生物学技术对细胞及细菌等微生物进行定性以及定量分析的研究方法,在国际上得到广泛的应用。本文介绍了应用FISH法对污水处理工艺中细菌数量的计数方...近年来,FISH(Fluorescence in Situ Hybridization,荧光原位杂交)法作为一种应用分子生物学技术对细胞及细菌等微生物进行定性以及定量分析的研究方法,在国际上得到广泛的应用。本文介绍了应用FISH法对污水处理工艺中细菌数量的计数方法。同时介绍了活性污泥试样制作、FISH法观测试样的制作以及各种试剂的配制、作用以及使用方法,基因探针的选择原则、杂交方法等实际操作方法与过程。展开更多
基金Supported by the Project of Basic Investigation on Ecological Environment Quality of Erhai Lake(No.TPDL-2021-C 265)the Ecological Effects,Population Regulation and Management Strategies of Invasion of Japanese Smelt(Hypomesus nipponensis)in Erhai Lake funded by the government of Dali City,Yunnan Province,China(No.[2018]447)。
文摘Environmental DNA(eDNA)has been used as an important tool for fish diversity analysis,which can greatly solve the problems in traditional survey methodology.However,little work has been done on the actual monitoring accuracy of eDNA.In this study,we analyzed the current status of fish resources in Erhai Lake in Yunnan,SW China,by dividing the lake into three sectors according to habitat differences,and compared the results of eDNA and traditional capture methods to investigate the shortcomings of the current analysis of eDNA results.A total of 27 fish species were detected by eDNA and traditional capture methods,including 20 and 19 fish species,respectively,and additional differences in fish composition between the two methods.The alpha diversity showed higher fish abundance and lower fish diversity by eDNA method compared to the traditional capture method,demonstrating that eDNA was not superior for use in fish diversity analysis.Fish community similarity analysis showed that community differences were generally significant for eDNA(P<0.05).RDA analysis indicated that environmental factors did not significantly affect fish communities monitored by the eDNA method.However,water temperature,aquatic plants,and water depth had significant(P<0.05)effects on fish communities in the traditional capture method,suggesting that eDNA results are insensitive to the effects of environmental factors.Our results illustrate the effectiveness of eDNA in fish identification and the issues in quantification compared to traditional capture methods.Therefore,combining eDNA with traditional methods is a more effective method for analyzing eDNA metabarcoding,following which the protocols of both quantitative methods can be designed to explore the regularity of eDNA quantification.
基金financially supported by the China Postdoctoral Science Foundation(Grant No.2019M661024]the Open Foundation of State Key Laboratory of Coastal and Offshore Engineering(Grant No.LP1901)。
文摘The fish cage design requires accurate predictions of long-term extreme loads and responses.Compared with the time-consuming full long-term analysis method integrating all the probability distribution of the short-term extremes,the environmental contour method gains much attention in predicting the long-term extreme values due to the less computational effort.This paper investigates the long-term extreme response of a fish cage using the environmental contour method.The fish cage is numerically simulated based on the lumped-mass method and the curved beam theory.Based on the one-dimensional(1D)and two-dimensional(2D)environmental contour,the extreme responses,including the surge and heave motions,mooring force,and vertical bending of the floater,are predicted for different return periods and compared with the full long-term analysis results.Results indicate that the 1D method greatly underestimates the extreme values.The 2D environmental contour method with a higher percentile level,namely90%,provides reasonable estimations and seems to be suitable for the long-term value analysis.Sensitivity studies show that the mooring arrangement and the bending stiffness have great effects on the bending moment and the mooring force and the mooring line pre-tension has minor effects on the fish cage response.
文摘"Give a man a fish and you feed him for a day;teach a man to fish and you feed him for a lifetime".This proverb now becomes the slogan of China's international aid.This paper is based on my previous empirical studies on China's"donor logic"of aid.Rather than looking at the motives of Chinese aid and how it is challenging the Western ideas,this paper focuses on policy discourse and tries to seek the recent trends and knowledge transformations of China's international cooperation of education.
文摘近年来,FISH(Fluorescence in Situ Hybridization,荧光原位杂交)法作为一种应用分子生物学技术对细胞及细菌等微生物进行定性以及定量分析的研究方法,在国际上得到广泛的应用。本文介绍了应用FISH法对污水处理工艺中细菌数量的计数方法。同时介绍了活性污泥试样制作、FISH法观测试样的制作以及各种试剂的配制、作用以及使用方法,基因探针的选择原则、杂交方法等实际操作方法与过程。
