Identifying a device and detecting a change in its position is critical for secure devices management in the Internet of Things(IoT).In this paper,a device management system is proposed to track the devices by using a...Identifying a device and detecting a change in its position is critical for secure devices management in the Internet of Things(IoT).In this paper,a device management system is proposed to track the devices by using audio-based location distinction techniques.In the proposed scheme,traditional cryptographic techniques,such as symmetric encryption algorithm,RSA-based signcryption scheme,and audio-based secure transmission,are utilized to provide authentication,non-repudiation,and confidentiality in the information interaction of the management system.Moreover,an audio-based location distinction method is designed to detect the position change of the devices.Specifically,the audio frequency response(AFR)of several frequency points is utilized as a device signature.The device signature has the features as follows.(1)Hardware Signature:different pairs of speaker and microphone have different signatures;(2)Distance Signature:in the same direction,the signatures are different at different distances;and(3)Direction Signature:at the same distance,the signatures are different in different directions.Based on the features above,amovement detection algorithmfor device identification and location distinction is designed.Moreover,a secure communication protocol is also proposed by using traditional cryptographic techniques to provide integrity,authentication,and non-repudiation in the process of information interaction between devices,Access Points(APs),and Severs.Extensive experiments are conducted to evaluate the performance of the proposed method.The experimental results show that the proposedmethod has a good performance in accuracy and energy consumption.展开更多
It has been claimed in the literature that impossibility of faster-than-light quantum communication has an origin of indistinguishability of ensembles with the same density matrix. We show that the two concepts are no...It has been claimed in the literature that impossibility of faster-than-light quantum communication has an origin of indistinguishability of ensembles with the same density matrix. We show that the two concepts are not related.We argue that even with an ideal single-atom-precision measurement, it is generally impossible to produce two ensembles with exactly the same density matrix; or to produce ensembles with the same density matrix, classical communication is necessary. Hence the impossibility of faster-than-light communication does not imply the indistinguishability of ensembles with the same density matrix.展开更多
The close relationship between semantics and pragmatics has made it difficult to set a clear boundary between them.Dicussion of the relationship can date back to Morris, whose semiotic trichotomy was taken up by Carna...The close relationship between semantics and pragmatics has made it difficult to set a clear boundary between them.Dicussion of the relationship can date back to Morris, whose semiotic trichotomy was taken up by Carnap. Leech, Levinson, Bach and Huang also tried to make a distinction between the two, but no consensus has been reached.展开更多
This paper focuses on essential distinctions between the major native dialects of English which are often divided by linguists into the three general categories of the British Isles dialects,those of North America and...This paper focuses on essential distinctions between the major native dialects of English which are often divided by linguists into the three general categories of the British Isles dialects,those of North America and those of Australasia.English learners might want to learn a standard accent,because maybe they need it in a play or maybe they want to impress their friends,or maybe they want to fit in better in a certain English speaking environment.The paper hopes to help Chinese English learners firstly isolate the distinctions among different but widely-used and officially-accepted accents and then speak good English by means of eliminating incorrect accent by imitating Standard English pronunciation.展开更多
Laparoscopic surgery is a difficult surgical procedure compared with laparotomy. In particular, considerable skills and care are required for thread knotting in laparoscopic surgery. In this paper, a method for automa...Laparoscopic surgery is a difficult surgical procedure compared with laparotomy. In particular, considerable skills and care are required for thread knotting in laparoscopic surgery. In this paper, a method for automatic identification of a laparoscopic surgical procedure for ligation and online distinction of an abnormality, defined as any unusual manipulation, in the identified surgical procedure is proposed. Ligation is divided into several individual surgical procedures, and on the basis of the threshold criteria, each surgical procedure is identified. Next, the identified surgical procedure, thread knotting, is classified as either normal or abnormal using a self-organizing map. Finally, to reduce surgical error, an abnormality warning system which warns detection of an unusual manipulation in the surgical procedure to the operator is constructed.展开更多
By observing the grammar systems of Chinese and English,it is found that the uses of tenses between the two languages are very different. It turns out that the Chinese expressions of tenses are more casual and variabl...By observing the grammar systems of Chinese and English,it is found that the uses of tenses between the two languages are very different. It turns out that the Chinese expressions of tenses are more casual and variable,without fixed rules,while for English,more regular. The distinct ways and patterns of expressing tenses between Chinese and English give rise to more frequent errors made by students. Thus,only by a thorough and profound understanding of these distinctions can we find a better way to master the uses of tenses,which requires a reasonable comparison of the tenses between Chinese and English.展开更多
By using multi-objective analysis method, five architectural tourist sites with Lingnan characteristics were selected. Through the analysis on relevant data obtained by GIS, spatial analysis research had been conducte...By using multi-objective analysis method, five architectural tourist sites with Lingnan characteristics were selected. Through the analysis on relevant data obtained by GIS, spatial analysis research had been conducted on competitiveness of Lingnan distinctive architectural tourist cultural sites in Guangdong Province and some conclusions had been obtained.展开更多
Using monthly mean sea ice velocity data obtained from the International Arctic Buoy Programme (IABP) for the period of 1979–1998 and the monthly mean NCEP/NCAR re-analysis dataset (1960–2002), we investigated t...Using monthly mean sea ice velocity data obtained from the International Arctic Buoy Programme (IABP) for the period of 1979–1998 and the monthly mean NCEP/NCAR re-analysis dataset (1960–2002), we investigated the spatiotemporal evolution of the leading sea ice motion mode (based on a complex correlation matrix constructed of normalized sea ice motion velocity) and their association with sea level pressure (SLP) and the predominant modes of surface wind field variability. The results indicate that the leading winter sea ice motion mode’s spatial evolution is characterized by two alternating and distinct sea ice modes, or their linear combination. One mode (M1) shows a nearly closed cyclonic or anti-cyclonic circulation anomaly in the Arctic Basin and its marginal seas, resembling to a large extent the response of sea ice motion to the Arctic Oscillation (AO), as many previous studies have revealed. The other mode (M2) displays a coherent cyclonic or anti-cyclonic circulation anomaly with its center close to the Laptev Sea, which has not been identified in previous observational studies. In fact, M1 and M2 respectively reflect the responses of sea ice motion to two predominant modes of winter surface wind variability north of 70 ? N, which well correspond, with slight differences, to the first two modes of EOF analysis of winter monthly mean SLP north of 70 ? N. These slight differences in SLP anomalies lead to a difference of M2 from the response of sea ice motion to the dipole anomaly. Although the AO significantly influences sea ice motion, it is not crucial for the existence of M1. The new sea ice motion mode (M2) has the largest variance and clearly differs from the response of winter monthly mean sea ice motion to the dipole anomaly in SLP fields, and corresponding SLP anomalies also show differences compared to the dipole anomaly. This study indicates that in the Arctic Basin and its marginal seas, slight differences in SLP anomaly patterns can force distinctly different sea ice motion anomalies.展开更多
Assessment of variety distinctness is important for both the registration and the protection of particular variety. However, the current testing system, which assesses a range of morphological characters of each pair ...Assessment of variety distinctness is important for both the registration and the protection of particular variety. However, the current testing system, which assesses a range of morphological characters of each pair of varieties grown side-by-side, is time-consuming and is not suitable for the assessment of hundreds of samples. The objective of this study was to develop a procedure for the assessment of wheat variety distinctness using simple sequence repeat(SSR) markers. A comparison between the molecular and morphological profile of 797 varieties was made. On the basis of the comparison, pairs of varieties with a genetic similarity value(GSV) ≤90% were deemed to be distinct, accounting for ~85% of varieties assessed in wheat regional trials. For the remaining ~15% of varieties, GSVs between different varieties were 〉90%, among which ~35% were not distinct and the other ~65% were distinct. Therefore, if given a GSV〉90%, the pairs of varieties should be morphologically assessed in the field. To avoid any errors in the assessments, we proposed the elimination of contaminant plants from the sample before comparing the varietal genotypes, scoring of the genotype at each locus with a pair of allele numbers when constructing a molecular profile, and faithfully recording two alleles at a non-homozygous locus. To reduce the workload and cost, a three-grade markers comparison among varieties is suggested. In addition, 80 SSR markers and a technical procedure for assessment of wheat variety distinctness have been proposed. Based on the procedure, the distinctness assessment of ~85% of all wheat varieties is completed in our laboratory annually. Consequently, total field assessment has been reduced considerably.展开更多
The objectives of this study were to construct a database of expressed sequence tag (EST)-simple sequence repeat (SSR) markers to identify lettuce cultivars. A set of 370 EST-SSR primer pairs were applied for fingerpr...The objectives of this study were to construct a database of expressed sequence tag (EST)-simple sequence repeat (SSR) markers to identify lettuce cultivars. A set of 370 EST-SSR primer pairs were applied for fingerprinting the lettuce cultivars. Fifty-eight EST-SSR markers showed hy-per-variability and were able to differentiate 92 cultivars. A total of 176 polymorphic amplified fragments were obtained by the 58 markers, and two to eight SSR alleles were detected for each l°Cus with an average of three alleles per locus. Average polymorphism information content (PIC) was 0.425, ranging from 0.022 to 0.743. Cluster analysis was based on Jaccard’s distance coefficients using the method of unweighted pair group. In this method we used arithmetical averages (UPGMA) algorithm categorized 4 major groups, which were in accordance to morphological traits. The eight cultivars of three groups with 100% genetic similarity through SSR analysis were investigated by phenotypic traits. These cultivars including these pairs are very similar in 27 morphological characteristics. Therefore, these EST-SSR markers could be used to select similar cultivars through management of reference collection to complement distinctiveness test of lettuce cultivars.展开更多
Distinctness, Uniformity and Stability (DUS) testing is the technical base of Plant Variety Protection (PVP) and the scientific basis for the approval of Plant Breeder's Rights (PBR). DUS Test Guidelines are no...Distinctness, Uniformity and Stability (DUS) testing is the technical base of Plant Variety Protection (PVP) and the scientific basis for the approval of Plant Breeder's Rights (PBR). DUS Test Guidelines are not only the technical manuals for the DUS testing authorities to conduct the testing, but also the technical standards for the competent authorities to examine the DUS of new varieties of plants. Tea plant, originated from Yunnan Province, China, is a very important woody cash species in the world. The International Union for the Protection of New Varieties of Plants (UPOV) DUS Test Guidelines for tea plant is the first Test Guidelines prepared by China for the UPOV. In this article, the subject, selection, and determination of characteristics, states of expression of characteristics and the selection of example varieties, assessment of the UPOV DUS Test Guidelines for tea plant were elucidated in detail. Finally, the proposal of PVP for tea plant in China was also proposed. The preparation of UPOV DUS Test Guidelines for tea plant will have important significance both for promoting the development of PVP and increasing the status of international PVP fields for China.展开更多
In this paper, we assessed the ecological and biodiversity status in the Bohai Sea through a quantitative survey on mac-rofaunal community at 25 stations in Laizhou Bay and adjacent waters in the autumn of 2006.We tes...In this paper, we assessed the ecological and biodiversity status in the Bohai Sea through a quantitative survey on mac-rofaunal community at 25 stations in Laizhou Bay and adjacent waters in the autumn of 2006.We tested the robustness and effectiveness of taxonomic distinctness as an ecological indictor by analyzing its correlation with species richness and natural environmental variables and by analyzing other ecological indicators (Shannon-Wiener H' and W statistics from Abundance Biomass Comparison curve).Results so obtained indicated that the benthic environment of the study waters in general is not under major impact of anthropogenic disturbance, but some stations in Laizhou Bay and along the coast of the Shandong Peninsula and even in the central Bohai Sea might be moderately disturbed and showed signs of ecological degradation.The taxonomic distinctness measures △+ and Λ+ were independent of sampling effort and natural environment factors and were compliant to other ecological indicators.Further application of the taxonomic distinctness indicator to assess marine biodiversity and ecosystem health on a larger regional scale with historical data seems promising.展开更多
The intertidal zone is a complex coastal area exposed to a wide variety of environmental factors. During low tide, rocky pools are exposed to air for several hours throughout the day, and environmental conditions such...The intertidal zone is a complex coastal area exposed to a wide variety of environmental factors. During low tide, rocky pools are exposed to air for several hours throughout the day, and environmental conditions such as temperature and salinity are highly variable. Fish communities that inhabit these tide pools are also exposed to environmental variability;therefore it is important to look at fish community structure in relation to important environmental factors such as temperature, salinity and dissolved oxygen on tide pools during spring tides, time of greater exposure to air. Therefore, the aim of the present study was to analyze the variation of the taxonomic distinctness of intertidal fish communities considering temperature, salinity and dissolved oxygen of the water that remains trapped in the tide pools during low tide. Visual census surveys were carried out monthly, from January to December 2015, in the intertidal zone of El Faro during spring tides on full moon days, when fluctuations of environmental variables are significant. Total extension of the census surveys was 156 × 5 m (780 m2). A total of 145 tide pools were sampled and divided in three groups (small, medium and large) according to size and depths that ranged from 20 to 45 cm. Temperature, salinity and dissolved oxygen fluctuate throughout the year of study. Thermal difference between open ocean and tide pools ranged from 3.5°C to 4.5°C. A total of 3757 organisms from 22 species, 12 families, four orders and one class were recorded. When comparing taxonomic distinctness and average taxonomic distinctness between months and tide pools of different sizes, values fell within the confidence intervals in the tunnel and were located close to the mean.展开更多
This paper presents a three-dimensional fully hydro-mechanical coupled distinct element study on fault reactivation and induced seismicity due to hydraulic fracturing injection and subsequent backflow process,based on...This paper presents a three-dimensional fully hydro-mechanical coupled distinct element study on fault reactivation and induced seismicity due to hydraulic fracturing injection and subsequent backflow process,based on the geological data in Horn River Basin,Northeast British Columbia,Canada.The modeling results indicate that the maximum magnitude of seismic events appears at the fracturing stage.The increment of fluid volume in the fault determines the cumulative moment and maximum fault slippage,both of which are essentially proportional to the fluid volume.After backflow starts,the fluid near the joint intersection keeps flowing into the critically stressed fault,rather than backflows to the wellbore.Although fault slippage is affected by the changes of both pore pressure and ambient rock stress,their contributions are different at fracturing and backflow stages.At fracturing stage,pore pressure change shows a dominant effect on induced fault slippage.While at backflow stage,because the fault plane is under a critical stress state,any minor disturbance would trigger a fault slippage.The energy analysis indicates that aseismic deformation takes up a majority of the total deformation energy during hydraulic fracturing.A common regularity is found in both fracturing-and backflow-induced seismicity that the cumulative moment and maximum fault slippage are nearly proportional to the injected fluid volume.This study shows some novel insights into interpreting fracturing-and backflowinduced seismicity,and provides useful information for controlling and mitigating seismic hazards due to hydraulic fracturing.展开更多
Brand construction plays an increasingly important role in marketing and competition in modern agricultural industry. There are problems in brand construction of tropical distinctive agricultural products in Hainan,in...Brand construction plays an increasingly important role in marketing and competition in modern agricultural industry. There are problems in brand construction of tropical distinctive agricultural products in Hainan,including less famous brand products,weak core competence,strengthening declaration and ignoring management as well as imperfect marketing system,therefore resource integration should be stressed to develop famous brand products and technological innovation should be strengthened to promote core competence of enterprise.展开更多
On the basis of the developmental status of Guangxi distinctive agriculture,the key selection of developing Guangxi distinctive agriculture is analyzed.Its distinctive agriculture contains distinctive plantation,disti...On the basis of the developmental status of Guangxi distinctive agriculture,the key selection of developing Guangxi distinctive agriculture is analyzed.Its distinctive agriculture contains distinctive plantation,distinctive forestry and distinctive breeding.Based on the analysis,two display indices,domestic comparative advantages index and domestic competitiveness,are selected to analyze the competitiveness of Guangxi distinctive agriculture.The results show that at present the overall competitiveness of Guangxi distinctive agriculture is strong,but its competitiveness is reflected on resource advantages and most of the resources are primary agro-products.Accordingly,in the future,Guangxi distinctive agriculture should adopt non-equilibrium developmental strategy;take the great-leap-forward developmental path;apply modern production elements to increase the competitiveness of Guangxi distinctive agriculture.It should lay stress on "three innovation",which includes organization innovation,developing distinctive agricultural firms ;technology innovation,enlarging the scope and scale of agricultural listed companies;financial fund-raising innovation,transforming traditional distinctive agriculture by using high and new technology.展开更多
Atmospheric lower-power pulsed microwave argon cold plasma jets are obtained by using coaxial transmission line resonators in ambient air.The plasma jet plumes are generated at the end of a metal wire placed in the mi...Atmospheric lower-power pulsed microwave argon cold plasma jets are obtained by using coaxial transmission line resonators in ambient air.The plasma jet plumes are generated at the end of a metal wire placed in the middle of the dielectric tubes.The electromagnetic model analyses and simulation results suggest that the discharges are excited resonantly by the enhanced electric field of surface plasmon polaritons.Moreover,for conquering the defect of atmospheric argon filamentation discharges excited by 2.45-GHz of continued microwave,the distinctive patterns of the plasma jet plumes can be maintained by applying different gas flow rates of argon gas,frequencies of pulsed modulator,duty cycles of pulsed microwave,peak values of input microwave power,and even by using different materials of dielectric tubes.In addition,the emission spectrum,the plume temperature,and other plasma parameters are measured,which shows that the proposed pulsed microwave plasma jets can be adjusted for plasma biomedical applications.展开更多
Reliable estimates of slope stability are essential for safe design and planning of road cut hill slopes which accommo-date a number of tourist destinations around the world. The failure of cut slopes along these hill...Reliable estimates of slope stability are essential for safe design and planning of road cut hill slopes which accommo-date a number of tourist destinations around the world. The failure of cut slopes along these hills puts human life in grave danger and it is also disastrous for the economy. In the present study, a section of 100 m high jointed basalt hill slope has been analyzed numerically in a distinct element code, which is apt for simulating the behavior for jointed rock. The analysis was carried out for both the dry and saturated conditions. The distinct element analysis of the hill slope demonstrates it to be marginally stable under dry condition, while for the saturated condition, the hill slope fails along well defined joint planes.展开更多
基金This work is supported by Demonstration of Scientific and Technology Achievements Transform in Sichuan Province under Grant 2022ZHCG0036National Natural Science Foundation of China(62002047).
文摘Identifying a device and detecting a change in its position is critical for secure devices management in the Internet of Things(IoT).In this paper,a device management system is proposed to track the devices by using audio-based location distinction techniques.In the proposed scheme,traditional cryptographic techniques,such as symmetric encryption algorithm,RSA-based signcryption scheme,and audio-based secure transmission,are utilized to provide authentication,non-repudiation,and confidentiality in the information interaction of the management system.Moreover,an audio-based location distinction method is designed to detect the position change of the devices.Specifically,the audio frequency response(AFR)of several frequency points is utilized as a device signature.The device signature has the features as follows.(1)Hardware Signature:different pairs of speaker and microphone have different signatures;(2)Distance Signature:in the same direction,the signatures are different at different distances;and(3)Direction Signature:at the same distance,the signatures are different in different directions.Based on the features above,amovement detection algorithmfor device identification and location distinction is designed.Moreover,a secure communication protocol is also proposed by using traditional cryptographic techniques to provide integrity,authentication,and non-repudiation in the process of information interaction between devices,Access Points(APs),and Severs.Extensive experiments are conducted to evaluate the performance of the proposed method.The experimental results show that the proposedmethod has a good performance in accuracy and energy consumption.
文摘It has been claimed in the literature that impossibility of faster-than-light quantum communication has an origin of indistinguishability of ensembles with the same density matrix. We show that the two concepts are not related.We argue that even with an ideal single-atom-precision measurement, it is generally impossible to produce two ensembles with exactly the same density matrix; or to produce ensembles with the same density matrix, classical communication is necessary. Hence the impossibility of faster-than-light communication does not imply the indistinguishability of ensembles with the same density matrix.
文摘The close relationship between semantics and pragmatics has made it difficult to set a clear boundary between them.Dicussion of the relationship can date back to Morris, whose semiotic trichotomy was taken up by Carnap. Leech, Levinson, Bach and Huang also tried to make a distinction between the two, but no consensus has been reached.
文摘This paper focuses on essential distinctions between the major native dialects of English which are often divided by linguists into the three general categories of the British Isles dialects,those of North America and those of Australasia.English learners might want to learn a standard accent,because maybe they need it in a play or maybe they want to impress their friends,or maybe they want to fit in better in a certain English speaking environment.The paper hopes to help Chinese English learners firstly isolate the distinctions among different but widely-used and officially-accepted accents and then speak good English by means of eliminating incorrect accent by imitating Standard English pronunciation.
文摘Laparoscopic surgery is a difficult surgical procedure compared with laparotomy. In particular, considerable skills and care are required for thread knotting in laparoscopic surgery. In this paper, a method for automatic identification of a laparoscopic surgical procedure for ligation and online distinction of an abnormality, defined as any unusual manipulation, in the identified surgical procedure is proposed. Ligation is divided into several individual surgical procedures, and on the basis of the threshold criteria, each surgical procedure is identified. Next, the identified surgical procedure, thread knotting, is classified as either normal or abnormal using a self-organizing map. Finally, to reduce surgical error, an abnormality warning system which warns detection of an unusual manipulation in the surgical procedure to the operator is constructed.
文摘By observing the grammar systems of Chinese and English,it is found that the uses of tenses between the two languages are very different. It turns out that the Chinese expressions of tenses are more casual and variable,without fixed rules,while for English,more regular. The distinct ways and patterns of expressing tenses between Chinese and English give rise to more frequent errors made by students. Thus,only by a thorough and profound understanding of these distinctions can we find a better way to master the uses of tenses,which requires a reasonable comparison of the tenses between Chinese and English.
基金Supported by Basic Tourism which is the Elaborate Course of Guang-zhou Vocational College of Technology and BusinessSubtopic of 863Key Program (2006AA06A306 )+1 种基金Key Item of Weather Bureau of Shandong Province (2009sdqxz16)Geochemical Investigation Data-base (INFO-115-C01-SDB4-06) ~~
文摘By using multi-objective analysis method, five architectural tourist sites with Lingnan characteristics were selected. Through the analysis on relevant data obtained by GIS, spatial analysis research had been conducted on competitiveness of Lingnan distinctive architectural tourist cultural sites in Guangdong Province and some conclusions had been obtained.
基金supported by Interactionsof the External Forcing in the Northern Mid-high Latitudes with Atmospheric Circulations (GYHY200906017)the Coordinated Observation and Prediction of Earth System(COPES) project (GYHY200706005)the National Natural Science Foundation of China (Grant No. 40875052),and the Alaska Ocean Observing System (AOOS)
文摘Using monthly mean sea ice velocity data obtained from the International Arctic Buoy Programme (IABP) for the period of 1979–1998 and the monthly mean NCEP/NCAR re-analysis dataset (1960–2002), we investigated the spatiotemporal evolution of the leading sea ice motion mode (based on a complex correlation matrix constructed of normalized sea ice motion velocity) and their association with sea level pressure (SLP) and the predominant modes of surface wind field variability. The results indicate that the leading winter sea ice motion mode’s spatial evolution is characterized by two alternating and distinct sea ice modes, or their linear combination. One mode (M1) shows a nearly closed cyclonic or anti-cyclonic circulation anomaly in the Arctic Basin and its marginal seas, resembling to a large extent the response of sea ice motion to the Arctic Oscillation (AO), as many previous studies have revealed. The other mode (M2) displays a coherent cyclonic or anti-cyclonic circulation anomaly with its center close to the Laptev Sea, which has not been identified in previous observational studies. In fact, M1 and M2 respectively reflect the responses of sea ice motion to two predominant modes of winter surface wind variability north of 70 ? N, which well correspond, with slight differences, to the first two modes of EOF analysis of winter monthly mean SLP north of 70 ? N. These slight differences in SLP anomalies lead to a difference of M2 from the response of sea ice motion to the dipole anomaly. Although the AO significantly influences sea ice motion, it is not crucial for the existence of M1. The new sea ice motion mode (M2) has the largest variance and clearly differs from the response of winter monthly mean sea ice motion to the dipole anomaly in SLP fields, and corresponding SLP anomalies also show differences compared to the dipole anomaly. This study indicates that in the Arctic Basin and its marginal seas, slight differences in SLP anomaly patterns can force distinctly different sea ice motion anomalies.
基金funded by the Innovation Platform for Basic Research in Breeding of Beijing, China (D08070500690801)the Science and Technology Innovation Ability Construction Projects,Beijing Academy of Agricultural and Forestry Sciences, China (KJCX20140202)
文摘Assessment of variety distinctness is important for both the registration and the protection of particular variety. However, the current testing system, which assesses a range of morphological characters of each pair of varieties grown side-by-side, is time-consuming and is not suitable for the assessment of hundreds of samples. The objective of this study was to develop a procedure for the assessment of wheat variety distinctness using simple sequence repeat(SSR) markers. A comparison between the molecular and morphological profile of 797 varieties was made. On the basis of the comparison, pairs of varieties with a genetic similarity value(GSV) ≤90% were deemed to be distinct, accounting for ~85% of varieties assessed in wheat regional trials. For the remaining ~15% of varieties, GSVs between different varieties were 〉90%, among which ~35% were not distinct and the other ~65% were distinct. Therefore, if given a GSV〉90%, the pairs of varieties should be morphologically assessed in the field. To avoid any errors in the assessments, we proposed the elimination of contaminant plants from the sample before comparing the varietal genotypes, scoring of the genotype at each locus with a pair of allele numbers when constructing a molecular profile, and faithfully recording two alleles at a non-homozygous locus. To reduce the workload and cost, a three-grade markers comparison among varieties is suggested. In addition, 80 SSR markers and a technical procedure for assessment of wheat variety distinctness have been proposed. Based on the procedure, the distinctness assessment of ~85% of all wheat varieties is completed in our laboratory annually. Consequently, total field assessment has been reduced considerably.
文摘The objectives of this study were to construct a database of expressed sequence tag (EST)-simple sequence repeat (SSR) markers to identify lettuce cultivars. A set of 370 EST-SSR primer pairs were applied for fingerprinting the lettuce cultivars. Fifty-eight EST-SSR markers showed hy-per-variability and were able to differentiate 92 cultivars. A total of 176 polymorphic amplified fragments were obtained by the 58 markers, and two to eight SSR alleles were detected for each l°Cus with an average of three alleles per locus. Average polymorphism information content (PIC) was 0.425, ranging from 0.022 to 0.743. Cluster analysis was based on Jaccard’s distance coefficients using the method of unweighted pair group. In this method we used arithmetical averages (UPGMA) algorithm categorized 4 major groups, which were in accordance to morphological traits. The eight cultivars of three groups with 100% genetic similarity through SSR analysis were investigated by phenotypic traits. These cultivars including these pairs are very similar in 27 morphological characteristics. Therefore, these EST-SSR markers could be used to select similar cultivars through management of reference collection to complement distinctiveness test of lettuce cultivars.
文摘Distinctness, Uniformity and Stability (DUS) testing is the technical base of Plant Variety Protection (PVP) and the scientific basis for the approval of Plant Breeder's Rights (PBR). DUS Test Guidelines are not only the technical manuals for the DUS testing authorities to conduct the testing, but also the technical standards for the competent authorities to examine the DUS of new varieties of plants. Tea plant, originated from Yunnan Province, China, is a very important woody cash species in the world. The International Union for the Protection of New Varieties of Plants (UPOV) DUS Test Guidelines for tea plant is the first Test Guidelines prepared by China for the UPOV. In this article, the subject, selection, and determination of characteristics, states of expression of characteristics and the selection of example varieties, assessment of the UPOV DUS Test Guidelines for tea plant were elucidated in detail. Finally, the proposal of PVP for tea plant in China was also proposed. The preparation of UPOV DUS Test Guidelines for tea plant will have important significance both for promoting the development of PVP and increasing the status of international PVP fields for China.
基金funded by the National Natural Science Foundation of China (Grant Nos.40730847,40906063 and 41076090)
文摘In this paper, we assessed the ecological and biodiversity status in the Bohai Sea through a quantitative survey on mac-rofaunal community at 25 stations in Laizhou Bay and adjacent waters in the autumn of 2006.We tested the robustness and effectiveness of taxonomic distinctness as an ecological indictor by analyzing its correlation with species richness and natural environmental variables and by analyzing other ecological indicators (Shannon-Wiener H' and W statistics from Abundance Biomass Comparison curve).Results so obtained indicated that the benthic environment of the study waters in general is not under major impact of anthropogenic disturbance, but some stations in Laizhou Bay and along the coast of the Shandong Peninsula and even in the central Bohai Sea might be moderately disturbed and showed signs of ecological degradation.The taxonomic distinctness measures △+ and Λ+ were independent of sampling effort and natural environment factors and were compliant to other ecological indicators.Further application of the taxonomic distinctness indicator to assess marine biodiversity and ecosystem health on a larger regional scale with historical data seems promising.
文摘The intertidal zone is a complex coastal area exposed to a wide variety of environmental factors. During low tide, rocky pools are exposed to air for several hours throughout the day, and environmental conditions such as temperature and salinity are highly variable. Fish communities that inhabit these tide pools are also exposed to environmental variability;therefore it is important to look at fish community structure in relation to important environmental factors such as temperature, salinity and dissolved oxygen on tide pools during spring tides, time of greater exposure to air. Therefore, the aim of the present study was to analyze the variation of the taxonomic distinctness of intertidal fish communities considering temperature, salinity and dissolved oxygen of the water that remains trapped in the tide pools during low tide. Visual census surveys were carried out monthly, from January to December 2015, in the intertidal zone of El Faro during spring tides on full moon days, when fluctuations of environmental variables are significant. Total extension of the census surveys was 156 × 5 m (780 m2). A total of 145 tide pools were sampled and divided in three groups (small, medium and large) according to size and depths that ranged from 20 to 45 cm. Temperature, salinity and dissolved oxygen fluctuate throughout the year of study. Thermal difference between open ocean and tide pools ranged from 3.5°C to 4.5°C. A total of 3757 organisms from 22 species, 12 families, four orders and one class were recorded. When comparing taxonomic distinctness and average taxonomic distinctness between months and tide pools of different sizes, values fell within the confidence intervals in the tunnel and were located close to the mean.
基金supported by the Key Innovation Team Program of Innovation Talents Promotion Plan by Ministry of Science and Technology of China(Grant No.2016RA4059)National Natural Science Foundation of China(Grant Nos.41672268 and 41772286)。
文摘This paper presents a three-dimensional fully hydro-mechanical coupled distinct element study on fault reactivation and induced seismicity due to hydraulic fracturing injection and subsequent backflow process,based on the geological data in Horn River Basin,Northeast British Columbia,Canada.The modeling results indicate that the maximum magnitude of seismic events appears at the fracturing stage.The increment of fluid volume in the fault determines the cumulative moment and maximum fault slippage,both of which are essentially proportional to the fluid volume.After backflow starts,the fluid near the joint intersection keeps flowing into the critically stressed fault,rather than backflows to the wellbore.Although fault slippage is affected by the changes of both pore pressure and ambient rock stress,their contributions are different at fracturing and backflow stages.At fracturing stage,pore pressure change shows a dominant effect on induced fault slippage.While at backflow stage,because the fault plane is under a critical stress state,any minor disturbance would trigger a fault slippage.The energy analysis indicates that aseismic deformation takes up a majority of the total deformation energy during hydraulic fracturing.A common regularity is found in both fracturing-and backflow-induced seismicity that the cumulative moment and maximum fault slippage are nearly proportional to the injected fluid volume.This study shows some novel insights into interpreting fracturing-and backflowinduced seismicity,and provides useful information for controlling and mitigating seismic hazards due to hydraulic fracturing.
文摘Brand construction plays an increasingly important role in marketing and competition in modern agricultural industry. There are problems in brand construction of tropical distinctive agricultural products in Hainan,including less famous brand products,weak core competence,strengthening declaration and ignoring management as well as imperfect marketing system,therefore resource integration should be stressed to develop famous brand products and technological innovation should be strengthened to promote core competence of enterprise.
基金Supported by 2010 Research Project of Guangxi Education Department(201010LX411)Research Project of Guangxi University of Finance and Economics(2010A01)
文摘On the basis of the developmental status of Guangxi distinctive agriculture,the key selection of developing Guangxi distinctive agriculture is analyzed.Its distinctive agriculture contains distinctive plantation,distinctive forestry and distinctive breeding.Based on the analysis,two display indices,domestic comparative advantages index and domestic competitiveness,are selected to analyze the competitiveness of Guangxi distinctive agriculture.The results show that at present the overall competitiveness of Guangxi distinctive agriculture is strong,but its competitiveness is reflected on resource advantages and most of the resources are primary agro-products.Accordingly,in the future,Guangxi distinctive agriculture should adopt non-equilibrium developmental strategy;take the great-leap-forward developmental path;apply modern production elements to increase the competitiveness of Guangxi distinctive agriculture.It should lay stress on "three innovation",which includes organization innovation,developing distinctive agricultural firms ;technology innovation,enlarging the scope and scale of agricultural listed companies;financial fund-raising innovation,transforming traditional distinctive agriculture by using high and new technology.
基金supported by the National Natural Science Foundation of China(Grant Nos.11105002 and 61170172)the Natural Science Foundation of Anhui Province,China(Grant Nos.1408085QA16 and 1408085ME101)+1 种基金the China Postdoctoral Science Foundation(Grant No.2014M551788)the Open-end Fund of State Key Laboratory of Advanced Electromagnetic Engineering and Technology(HUST),China(Grant No.GZ1301)
文摘Atmospheric lower-power pulsed microwave argon cold plasma jets are obtained by using coaxial transmission line resonators in ambient air.The plasma jet plumes are generated at the end of a metal wire placed in the middle of the dielectric tubes.The electromagnetic model analyses and simulation results suggest that the discharges are excited resonantly by the enhanced electric field of surface plasmon polaritons.Moreover,for conquering the defect of atmospheric argon filamentation discharges excited by 2.45-GHz of continued microwave,the distinctive patterns of the plasma jet plumes can be maintained by applying different gas flow rates of argon gas,frequencies of pulsed modulator,duty cycles of pulsed microwave,peak values of input microwave power,and even by using different materials of dielectric tubes.In addition,the emission spectrum,the plume temperature,and other plasma parameters are measured,which shows that the proposed pulsed microwave plasma jets can be adjusted for plasma biomedical applications.
文摘Reliable estimates of slope stability are essential for safe design and planning of road cut hill slopes which accommo-date a number of tourist destinations around the world. The failure of cut slopes along these hills puts human life in grave danger and it is also disastrous for the economy. In the present study, a section of 100 m high jointed basalt hill slope has been analyzed numerically in a distinct element code, which is apt for simulating the behavior for jointed rock. The analysis was carried out for both the dry and saturated conditions. The distinct element analysis of the hill slope demonstrates it to be marginally stable under dry condition, while for the saturated condition, the hill slope fails along well defined joint planes.