Efficacy and Safety of Iron Isomaltoside Compared with an Oral Iron Supplement in the Management of Patients with Iron Deficiency Anemia

Objective: To evaluate the treatment outcome of iron isomaltoside compared with an oral iron supplement in the management of iron deficiency anemia (IDA). Methods: The study included patients with IDA who visited the Outpatient Clinic of the Department of Hematology, the Affiliated Hospital of Qingdao University from October 2021 to August 2022 and met the inclusion and exclusion criteria. According to the actual application of iron supplementation, the patients were divided into two groups: iron isomaltoside treatment group and oral iron treatment group. Baseline measurements were collected before the start of treatment, and measurements were collected subsequently at intervals of 1 week, 1 month, and 3 months. The hematological parameters analyzed included Hemoglobin (Hb), Mean corpuscular hemoglobin (MCH), Mean Hemoglobin content (MCH), Mean corpuscular Hemoglobin concentration (MCHC), and Platelet (Plt). Safety data and adverse event profiles were recorded. Results: Intra-group comparisons: After 1 month of treatment, the Hb significantly improved (P 0.05). Inter-group comparisons: The biochemical parameters were significantly improved (P 0.05) in the iron isomaltoside group compared with those in the oral iron group after 1 month of iron supplementation in patients with mild and moderate anemia. Adverse reactions were tolerable for the patients in both iron isomaltoside group and oral iron group. Only 1 patient in iron isomaltoside group developed anaphylactic shock during medication and recovered after aggressive rescue. Conclusions: Iron isomaltoside which increases Hb more rapidly compared with the oral iron supplementation has few adverse reactions and good acceptance.

Responses of growth performance,antioxidant function,small intestinal morphology and mRNA expression of jejunal tight junction protein to dietary iron in yellow-feathered broilers

This study aimed to investigate the dose-effect of iron on growth performance,antioxidant function.intestinal morphology,and mRNA expression of jejunal tight junction protein in 1-to21-d-old yellow-feathered broilers.A total of 7201-d-old yellow-feathered maleb roilers were allocated to 9 treatments with 8 replicate cages of 10 birds per cage.The dietary treatments were consisted of a basal diet(contained 79.6 mg Fe kg^(-1))supplemented with 0,20,40,60,80,160,320,640,and 1,280 mg Fe kg^(-1)in the form of FeSO_(4)·7H_(2)O.Compared with the birds in the control group,birds supplemented with 20mg Fe kg^(-1)had higher average daily gain(ADG)(P<0.0001).Adding 640 and 1,280 mg Fe kg^(-1)significantly decreased ADG(P<0.0001)and average daily feed intake(ADFI)(P<0.0001)compared with supplementation of 20mg Fe kg^(-1).Malondialdehyde(MDA)concentration in plasma and duodenum increased linearly(P<0.0001),but MDA concentration in liver and jejunum increased linearly(P<0.05)or quadratically(P<0.05)with increased dietary Fe concentration.The villus height(VH)in duodenum and jejunum,and the ratio of villus height to crypt depth(V/C)in duodenum decreased linearly(P?0.05)as dietary Feincreased.As dietary Fe increased,the jejunal relative mRNA abundance of claudin-1 decreased linearly(P=0.001),but the jejunal relative mRNA abundance of zona occludens-1(ZO-1)and occludin decreased linearly(P?0.05)or quadratically(P?0.05).Compared with the supplementation of 20 mg Fe kg^(-1),the supplementation of640 mg Fe kg^(-1)or higher increased(P?0.05)MDA concentrations in plasma,duodenum,and jejunum,decreased VH in the duodenum and jejunum,and the addition of 1,280 mg Fe kg^(-1)reduced(P?0.05)the jejunal tight junction protein(claudin-1,ZO-1,occludin)mRNA abundance.In summary,640 mg of supplemental Fe kg^(-1)or greater was associated with decreased growth performance,increased oxidative stress,disrupted intestinal morphology,and reduced mRNA expression of jejunal tight junction protein.

Enhanced recovery of high-purity Fe powder from iron-rich electrolytic manganese residue by slurry electrolysis

Iron-rich electrolytic manganese residue(IREMR)is an industrial waste produced during the processing of electrolytic metal manganese,and it contains certain amounts of Fe and Mn resources and other heavy metals.In this study,the slurry electrolysis technique was used to recover high-purity Fe powder from IREMR.The effects of IREMR and H2SO4 mass ratio,current density,reaction temper-ature,and electrolytic time on the leaching and current efficiencies of Fe were studied.According to the results,high-purity Fe powder can be recovered from the cathode plate,and the slurry electrolyte can be recycled.The leaching efficiency,current efficiency,and purity of Fe reached 92.58%,80.65%,and 98.72wt%,respectively,at a 1:2.5 mass ratio of H2SO4 and IREMR,reaction temperature of 60℃,electric current density of 30 mA/cm^(2),and reaction time of 8 h.In addition,vibrating sample magnetometer(VSM)analysis showed that the coercivity of electrolytic iron powder was 54.5 A/m,which reached the advanced magnetic grade of electrical pure-iron powder(DT4A coercivity standard).The slurry electrolytic method provides fundamental support for the industrial application of Fe resource recovery in IRMER.

Revolutionizing disease diagnosis and management:Open-access magnetic resonance imaging datasets a challenge for artificial intelligence driven liver iron quantification

Artificial intelligence(AI),particularly machine learning(ML)and deep learning(DL)techniques,such as convolutional neural networks(CNNs),have emerged as transformative technologies with vast potential in healthcare.Body iron load is usually assessed using slightly invasive blood tests(serum ferritin,serum iron,and serum transferrin).Serum ferritin is widely used to assess body iron and drive medical management;however,it is an acute phase reactant protein offering wrong interpretation in the setting of inflammation and distressed patients.Magnetic resonance imaging is a non-invasive technique that can be used to assess liver iron.The ML and DL algorithms can be used to enhance the detection of minor changes.However,a lack of open-access datasets may delay the advancement of medical research in this field.In this letter,we highlight the importance of standardized datasets for advancing AI and CNNs in medical imaging.Despite the current limitations,embracing AI and CNNs holds promise in revolutionizing disease diagnosis and treatment.

Inpatient management of iron deficiency anemia in pediatric patients with inflammatory bowel disease: A single center experience

BACKGROUND Screening for iron deficiency anemia(IDA)is important in managing pediatric patients with inflammatory bowel disease(IBD).Concerns related to adverse reactions may contribute to a reluctance to prescribe intravenous(IV)iron to treat IDA in this population.AIM To track the efficacy and safety of IV iron therapy in treating IDA in pediatric IBD patients admitted to our center.METHODS A longitudinal observational cohort study was performed on 236 consecutive pediatric patients admitted to our tertiary IBD care center between September 2017 and December 2019.92 patients met study criteria for IDA,of which 57 received IV iron,17 received oral iron,and 18 were discharged prior to receiving iron therapy.RESULTS Patients treated with IV iron during their hospitalization experienced a significant increase of 1.9(±0.2)g/dL in mean(±SE)hemoglobin(Hb)concentration by the first ambulatory follow-up,compared to patients who received oral iron 0.8(±0.3)g/dL or no iron 0.8(±0.3)g/dL(P=0.03).One out of 57(1.8%)patients that received IV iron therapy experienced an adverse reaction.CONCLUSION Our findings demonstrate that treatment with IV iron therapy is safe and efficacious in improving Hb and iron levels in pediatric patients with IDA and active IBD.

Operation and Management of Electrical Equipment in Iron and Steel Enterprises

How to ensure the reliable operation of the complex and huge electrical system composed of a large number of electrical equipment in iron and steel enterprises?Combined with working experience,the author introduces four main factors affecting the normal operation of equipment,analyzes five main problems existing in the operation and management of electrical equipment,and puts forward corresponding improvement measures,so as to improve the management level of electrical equipment in iron and steel enterprises.

牛冠状病毒TaqMan荧光定量PCR检测方法的建立及应用

为建立牛冠状病毒TaqMan荧光定量PCR检测方法,根据GenBank收录的牛冠状病毒AKS-01株N基因序列(KU886219)保守区设计特异性引物和探针,构建重组质粒并进行反应条件优化、特异性试验、重复性试验以及敏感性试验,建立一种检测BCoV的TaqMan荧光定量PCR方法。结果显示,建立的牛冠状病毒TaqMan荧光定量PCR检测方法特异性、敏感性和重复性均良好。该方法BCoV重组质粒标准品在5.75×10^(7)~5.75×10^(3)copies/μL时与Ct值呈现良好线性关系,该方法对牛轮状病毒、牛传染性鼻气管炎病毒、牛病毒性腹泻病毒、牛副流感病毒3型均无交叉反应,特异性良好;该方法对BCoV重组质粒标准品最低检测限为5.75×10^(1)copies/μL;批内和批间重复性试验结果稳定,变异系数均小于2%。利用所建立的TaqMan荧光定量PCR方法对收集的132份样品进行检测,与常规PCR相比,两者符合率为96.21%,可为BCoV的临床检测和流行病学调查提供技术支持。

鸡毒支原体 Taq Man实时荧光定量PCR检测方法的建立

为快速检测鸡毒支原体,根据鸡毒支原体的保守基因设计特异性引物和Taq Man探针,建立鸡毒支原体Taq Man实时荧光定量PCR方法并分析鸡毒支原体培养过程中颜色单位改变法(color change unit,CCU)和荧光定量PCR检测的相关性。结果显示,标准曲线y=-3.646x+44.208,相关系数R^(2)=0.998,最低检测限度为1 copy/μL;与其他菌株等均无交叉反应;组内和组间变异系数均小于3%,表明该方法具有良好的稳定性和可重复性。用建立的实时荧光定量PCR检测方法对26份临床样品进行检测,该方法较普通PCR方法检出率更高。建立的荧光定量PCR与CCU显示鸡毒支原体在对数生长期时,两者具有一定的对应关系。表明建立了鸡毒支原体Taq Man探针实时荧光定量PCR检测方法,可实现对鸡毒支原体的快速检测,为鸡毒支原体感染的防控提供技术基础。

Improvement of hydrogen permeation barrier performance by iron sulphide surface films

Fe–S compounds with hexagonal crystal structure are potential hydrogen permeation barrier during H2S corrosion. Hexagonal system Fe–S films were prepared on carbon steel through corrosion and CVD deposition, and the barrier effect of different Fe–S films on hydrogen permeation was tested using electrochemical hydrogen permeation method. After that, the electrical properties of Fe–S compound during phase transformation were measured using thermoelectric measurement system. Results show that the mackinawite has no obvious barrier effect on hydrogen penetration, as a p-type semiconductor, and pyrrhotite (including troilite) has obvious barrier effect on hydrogen penetration,as an n-type semiconductor. Hydrogen permeation tests showed peak permeation performance when the surface was deposited with a continuous film of pyrrhotite (Fe_(1–x)S) and troilite. The FeS compounds suppressed hydrogen permeation by the promotion of the hydrogen evolution reaction, semiconducting inversion from p-to n-type, and the migration of ions at the interface.

羊贝氏柯克斯体TaqMan荧光定量PCR检测方法的建立及应用

为建立检测贝氏柯克斯体(Coxiella burnetii)实时荧光定量PCR方法,根据GenBank上收录的C.burnetii RSA439株com1基因序列(CP040059.1)保守区设计特异性引物和探针,并构建重组质粒及优化反应条件,以期建立一种检测贝氏柯克斯体的Taq Man荧光定量PCR方法。结果显示,该方法所建立标准曲线线性关系良好;敏感性试验结果显示,最低检出限为2.45×10^(2) copies/μL,与PCR最低检出限为2.45×10^(4) copies/μL相比较,灵敏度提高了100倍;对羊支原体、羊流产衣原体、羊布鲁氏菌等均无交叉反应,批内和批间重复性试验结果稳定,变异系数均小于3%,说明特异性、重复性好;利用该方法对收集的126份样品进行检测,结果显示样品阳性率为9.52%,常规PCR检测阳性率为7.14%,符合率为90.47%。该方法可为贝氏柯克斯体临床上快速检测及防控提供技术支持。

牛支原体和丝状支原体丝状亚种双重TaqMan荧光定量PCR检测方法的建立与应用

牛传染性胸膜肺炎和牛支原体病均为重要的牛传染病,两者均可致牛出现呼吸系统症状,临床上难以区分。为建立鉴别诊断牛传染性胸膜肺炎和牛支原体病的双重Taq Man荧光定量PCR检测方法,本研究根据这两种病的病原丝状支原体丝状亚种和牛支原体的基因组保守区域(丝状支原体丝状亚种nt826-nt1742,牛支原体nt189-nt618)分别设计引物与探针,通过优化反应体系与反应条件,建立同时检测这两种病原的Taq Man荧光定量PCR检测方法。特异性试验结果显示,该方法仅对丝状支原体丝状亚种模拟样品和牛支原体检测结果为阳性,而巴氏杆菌、牛传染性鼻气管炎病毒、无乳支原体、山羊支原体山羊肺炎亚种、丝状支原体山羊亚种、关节炎支原体Leachii株检测结果均为阴性,特异性较强。分别以1.0×10~7拷贝/μL~1.0×10~1拷贝/μL的p EASY-Mmm和p EASY-Mb质粒标准品为模板,进行敏感性试验,结果显示,该方法对丝状支原体丝状亚种和牛支原体重组质粒标准品的检测限均为1.0×10~1拷贝/μL,敏感性较高。对不同浓度质粒标准品混合物的重复性试验结果显示,组内与组间重复性试验变异系数均小于2.5%,重复性较好。利用该方法对112份临床样品(104份鼻拭子、8份肺组织样品)检测,结果显示,丝状支原体丝状亚种检测结果和已发表PCR方法检测结果一致,均为阴性,而本实验建立的方法检测出4份牛支原体阳性样品,且经测序证实检出样品为真实阳性样品,而已发表的多重PCR方法检测该病原结果均为阴性。本研究建立了能够同时检测丝状支原体丝状亚种和牛支原体的双重Taq Man荧光定量PCR方法,其特异性强、敏感性高、重复性好,可用于各种临床样品的检测,为这两种病原的快速检测和流行病学调查提供了技术手段。

大口黑鲈弹状病毒Taq Man荧光定量PCR检测方法的建立及初步应用

为建立快速、敏感的大口黑鲈弹状病毒(MSRV)流行株Taq Man荧光定量PCR (qPCR)方法,本研究采用NCBI分析MSRV流行株N基因的保守序列,设计特异性引物和Taq Man探针,以构建的并经PCR和测序鉴定正确的重组质粒标准品p MD18-MSRV-N为模板,通过优化各反应条件初步建立MSRV的Taq Man q PCR检测方法。结果显示,建立的Taq Man q PCR方法标准曲线的R2为0.9906,质粒标准品在6.79×10^(8)拷贝/μL~6.79×10^(2)拷贝/μL范围内与Ct值均存在较好的线性关系。采用该方法检测MSRV及相关病毒,评估该方法的特异性,结果显示,该方法可以特异性检测MSRV,而草鱼出血病病毒、神经坏死病毒、鲤春病毒血症病毒、大口黑鲈双RNA病毒、鳜蛙虹彩病毒、传染性脾肾坏死病毒、石斑鱼虹彩病毒、大口黑鲈蛙虹彩病毒等8种常见鱼类病毒检测结果均为阴性;采用建立的方法检测不同浓度的质粒标准品(6.79×10^(8)拷贝/μL~6.79×10^(1)拷贝/μL),评估该方法的敏感性,结果显示,该方法的检测限为6.79×10^(2)拷贝/μL,比文献中的常规PCR方法敏感1 000倍;以不同批次或同一批次提取的不同浓度的质粒标准品作为模板,分别利用该方法检测,评估该方法的重复性,结果显示,该方法批内和批间重复性试验的变异系数均小于2%。采用该方法和文献中的常规PCR同时检测71份临床发病的大口黑鲈、鳜鱼、鲮鱼组织样品,结果显示阳性率为16.9%(12/71),阴性率为83.1%(59/71),且阳性样品均是从发病大口黑鲈的组织样品中检测到,而发病的鳜鱼、鲮鱼组织样品中均未检测到该病毒;常规PCR方法的阳性率为9.9%(7/71),阴性率为90.1%(64/71),二者的总符合率为93.0%。本研究建立的MSRV Taq Man q PCR方法特异性强、敏感性高、重复性和准确性均较好,可用于大口黑鲈临床样品的检测,为MSRV的快速检测提供了可行的技术手段。

猪肺炎支原体和猪鼻支原体TaqMan双重荧光PCR检测方法的建立及应用

为建立一种可快捷鉴别检测猪肺炎支原体(Mhp)和猪鼻支原体(Mhr)的方法,针对Mhp的183基因和Mhr的p37基因保守片段,分别设计合成引物及TaqMan探针,优化反应条件,建立一种可同时检测Mhp和Mhr的TaqMan双重荧光PCR方法,并评价其特异性、敏感性和重复性。结果表明,该方法特异性强,检测其他常见猪呼吸系统病原不发生交叉反应;对标准品pMD18-T-Mhp-183、pMD18-T-Mhr-P37的最低检测限分别达45.2 copies/μL和29.7 copies/μL,比普通PCR检测灵敏度提高10^(3)~10^(4)倍;该方法检测结果的批内与批间变异系数均小于2%。用该方法检测145份广西自治区内临床样品,从中检出82份Mhp和9份Mhr阳性样品。该方法可用于临床样品快速检测及实验室Mhp和Mhr的鉴定,可为Mhp和Mhr在猪群中的早期监测提供技术支持。

Cohort Study on Benefit of Martial Supplementation with EDTA Sodium Iron in Management of Gravidic Anemia at the University Clinics of Kinshasa

Background: Anemia is mainly attributed to nutritional deficiency, especially iron deficiency, which predominates during pregnancy, and is associated with parasitic diseases such as malaria and intestinal parasitosis, acute or chronic diseases such as sickle cell disease, tuberculosis, HIV infection and various micronutrients disorders. It is associated with an increased risk of low birth weight and prematurity and can contribute to impaired cognitive development in early childhood, as well as to maternal mortality. The impact on the fetus is even greater if maternal anemia onset is at an early stage, or prior to pregnancy. Iron salts, such as sulfate or fumarate, are widely used in the treatment of gravidic anemia, but due to various gastrointestinal side effects, many pregnant women are not compliant with treatment. Sodium iron EDTA, on the other hand, is highly absorbable and bioavailable. The latter improves hematological values and appears to be free from the usual iron-related side effects, hence compliance with this supplementation. Objectives: To evaluate the evolution of hemoglobin levels after sodium iron EDTA supplementation at the university Clinics of Kinshasa, to determine the frequency of gravidic anemia, the dose-dependent benefit of sodium iron EDTA supplementation in pregnant women and to identify factors associated with insufficient hemoglobin gain after supplementation. Methods: This longitudinal cohort will take place in the gynecology department of the University Clinics of Kinshasa from September 2022 to August 2023 and will include at least 54 pregnant women with anemia. Conclusion: The study will enable us to better assess the benefits of sodium iron EDTA in improving hematological values, as well as its tolerability in pregnant women suffering from anemia during pregnancy in our environment.

circMAN1A2调节miR-212-3p/DDIT4轴对胃癌细胞增殖、侵袭和迁移的影响

目的探究circMAN1A2靶向miR-212-3p/DNA损伤诱导转录子4(NDA damage-inducible transcript 4,DDIT4)轴对胃癌细胞增殖、侵袭和迁移的影响。方法qRT-PCR法检测胃癌组织和细胞中circMAN1A2、miR-212-3p表达水平。荧光素酶检测circMAN1A2与miR-212-3p、DDIT4和miR-212-3p的靶向关系。在胃癌细胞SGC-7901、HGC-27中转染si-NC、si-circMAN1A2、si-circMAN1A2+anti-NC、si-circMAN1A2+anti-miR-212-3p、miR-NC、miR-212-3p mimics,将未转染的SGC-7901、HGC-27细胞设为对照。平板克隆实验检测细胞增殖;细胞划痕及Transwell实验分别检测细胞迁移、侵袭能力。Western blotting检测增殖、迁移及侵袭相关蛋白表达。结果在胃癌组织及细胞中,circMAN1A2高表达,miR-212-3p低表达;下调circMAN1A2可以靶向上调miR-212-3p表达,且下调DDIT4表达,进而抑制胃癌细胞增殖、侵袭和迁移能力。抑制miR-212-3p表达可部分逆转抑制circMAN1A2表达对胃癌细胞恶性增殖的抑制作用。结论在胃癌细胞中抑制circMAN1A2表达可抑制胃癌细胞增殖、迁移与侵袭,其与调节miR-212-3p/DDIT4信号轴有关。

猪细小病毒TaqMan荧光定量PCR检测方法的建立及应用

旨在建立一种猪细小病毒(PPV)快速准确PCR检测方法,根据PPV VP2基因的保守序列设计并合成1对特异性引物和1条特异性探针,建立了可检测PPV的TaqMan荧光定量PCR检测方法。建立的检测方法敏感性高,最低可检测8.76×10^(1)copies/μL的PPV VP2基因标准品;特异性试验结果显示,该方法检测猪流行性腹泻病毒、猪圆环病毒2型、伪狂犬病病毒、猪繁殖与呼吸综合征病毒均无交叉反应;重复性试验组内与组间变异系数均低于2%,重复性良好。用该方法检测102份临床样本,检出阳性样本16份,阳性检出率为15.69%,将检测出的阳性样本进行PCR扩增并测序,证实检测样品中确实存在PPV。该方法敏感性高、特异性强,适用于PPV的定量检测及猪细小病毒病的流行病学调查。

An amorphous manganese iron oxide hollow nanocube cathode for aqueous zinc ion batteries

Aqueous zinc ion batteries(ZIBs) are attracting considerable attentions for practical energy storage because of their low cost and high safety.Nevertheless,the traditional manganese oxide cathode materials suffer from the low intrinsic electronic conductivity,sluggish ions diffusion kinetics,and structural collapse,hindering their large-scale application.Herein,we successfully developed a latent amorphous Mn_(1.8)Fe_(1.2)O_(4) hollow nanocube(a-H-MnFeO) cathode material derived from Prussian blue analogue precursor.The amorphous nature endows the cathode with lower diffusion barrier and narrower band gap compared with crystalline counterpart,resulting in the superior Zn^(2+) ions and electrons transport kinetics.Hollow structure can furnish abundant surface sites and suppress the structural collapse during the repeated charge/discharge processes.By virtue of the multiple advantageous features,the a-H-MnFeO cathode exhibits exceptional electrochemical performance,in terms of high capacity,excellent rate capability,and prolonged cycle life.This strategy will pave the way for the structural design of emerging cathode materials.

Iron and Heart Failure: Current Concepts and Emerging Pharmacological Paradigms

Background: Emerging evidence has recognized that anemia and iron deficiency are recurrent comorbidities in chronic heart failure (HF) and several trials have established that iron administration improves myocardial asset and clinical scenario in HF. Purpose: Recent acquisitions suggest that iron deficiency represents a concrete bias in the pathogenetic mechanism of chronic HF, so we have investigated the putative role of the hepcidin/ferroportin axis in the cardiovascular setting to advocate novel pharmacological and clinical approaches. Methods: Here, after an excursus on iron metabolism, we first reviewed the ongoing studies on novel iron targeted compounds. Then, we summarize large clinical interventional studies conducted on patient suffering from iron deficiency and HF which have tested the effects of drugging iron regard QoL, hospitalizations and cardiovascular death. Results: Novel compounds such as hepcidin agonist (PTG 300), synthetic human hepcidin (LJPC-401) and anti FPN (Vamifeport) are ongoing in iron overloaded patients, while the hepcidin blocker (PRS-080) is under investigation in anemic patients. Noteworthy, novel insights could arise from the results of a Phase IV interventional study regarding the modification of hepcidin pathway in a large cohort of HF patients (n = 1992) by sodium glucose cotransporter 2 inhibitors. To date, several studies highlight the beneficial effect of iron administration in cardiovascular setting and latest evidences consider hepcidin level as a novel biomarker of cardiac injury and atherosclerosis. Conclusions: We advocate that data from ongoing studies will suggest novel iron targeted therapies for diagnosis, prognosis and therapy transferable in selected heart failed patients.

TaqMan探针双重实时荧光定量PCR法同步检测兰花中的建兰花叶病毒和齿兰环斑病毒

为建立一种同步定量检测建兰花叶病毒(cymbidium mosaic virus,CymMV)和齿兰环斑病毒(odontoglossum ringspot virus,ORSV)的高效检测方法,本研究根据CymMV和ORSV CP基因高度保守区分别设计引物和探针并筛选,获得156 bp和148 bp的靶标序列及对应的最优特异性引物探针组合,建立了基于TaqMan探针的双重实时荧光定量PCR方法。该方法最低检出限为1拷贝或6.2×10^(-3)fg,最低稳定检出限为10拷贝或6.2×10^(-2)fg,是RT-PCR的10~100倍;构建的标准曲线线性关系良好,扩增效率分别为97.7%和100.2%,相关系数R2分别为1.000和0.999;对其他5种常见病毒均无扩增曲线,检测特异性强;批组内与批组间重复性试验Ct值变异系数≤0.60%,重复性和稳定性好。利用该方法和基因芯片法分别对4个种属的66个兰花样品进行方法验证,该方法相较于基因芯片法检出率提高了53.85%(CymMV)和162.5%(ORSV)。综上所述,本方法可同时高通量检测CymMV和ORSV 2种病毒靶基因,结果可靠,应用前景广阔,可为开展病毒精准鉴定、科学防控以及从源头遏制病毒传播提供技术支撑。

Parenteral iron therapy in children with iron deficiency anemia

Iron deficiency anemia(IDA)continues to be a global public health problem.Oral iron is the universally accepted first-line therapy,and most children have a prompt and favorable response to oral formulations.In subsets of children who fail to respond due to intolerance,poor adherence,or inadequate intestinal absorption,parenteral iron is indicated.Despite numerous studies in adults with IDA of diverse etiologies,pediatric studies on parenteral iron use are very limited.Although mostly retrospective and small,these studies have documented the efficacy and safety profile of intravenous iron formulations.In this editorial the author comments on the most important published data and underscores the need to seriously consider parenteral iron use in children unresponsive to oral therapy.