建鲤(Cyprinus carpio var. Jian)微卫星DNA亲权鉴定

利用16个微卫星座位对建鲤10个全同胞家系647个子代进行亲权鉴定。Cervus3.0分析表明,16个微卫星位点的平均多态信息含量为0.7025,平均等位基因数为6.63,期望杂合度平均为0.7405。当双亲未知时,累积排除概率为0.999225,已知单亲时的累积排除概率为0.999996,置信度为95%。进一步模拟分析表明,要达到亲权鉴定的要求在双亲未知时通常需要8～12个微卫星位点,已知单亲时需要5～8个微卫星位点。在双亲均未知的情况下进行亲权鉴定,94.6%的后裔找到了其父母本,真实鉴定率低于模拟分析预测值,分析可能是与候选亲本间存在亲缘关系、无效等位基因的存在以及分型错误等因素有关。9个建鲤全同胞家系的鉴定,为今后的遗传图谱构建、QTL定位及分子标记辅助育种研究奠定了基础。

油酸诱导建鲤(Cyprinus carpio var. Jian)肝细胞脂肪变性模型的建立

通过对油酸诱导时间及有效作用浓度的研究,建立油酸致建鲤肝细胞脂肪变性模型。本研究以胰蛋白酶消化法获得的原代肝细胞作为实验材料,加入不同浓度油酸(0、0.05、0.1、0.2、0.4、0.8 mmol/L)与肝细胞共培养24–48 h,诱导肝细胞脂肪变性,分别收集不同时段的肝细胞及上清液,采用试剂盒测定肝细胞中甘油三酯(TG)、总胆固醇(TC)的含量,同时测定上清培养液中谷丙转氨酶(GPT)、谷草转氨酶(GOT)、γ-谷氨酰转肽酶(γ-GT)、乳酸脱氢酶(LDH)和超氧化物歧化酶(SOD)的活性,MTT法测定肝细胞的存活情况,确定油酸最佳诱导浓度,油红O染色法观察细胞内脂肪滴的形成情况。研究结果显示,0.4 mmol/L油酸与肝细胞共培养48 h,可以显著提高肝细胞内TG、TC含量(P﹤0.05或P﹤0.01),极显著提高上清培养液中GOT、GPT、LDH、γ-GT的活性,显著降低上清培养液中SOD的活性,但与24 h相比有升高趋势,0.4 mmol/L油酸与肝细胞共培养24 h,光镜下可见细胞内有脂肪滴形成,以48 h最明显。综上所述,油酸浓度0.4 mmol/L、诱导时间48 h成功构建了肝细胞脂肪变性模型,为研究鱼类脂肪肝类疾病奠定了基础。

周期性饥饿再投喂对建鲤(Cyprinus carpio var.Jian)生长、体组成、消化酶的影响

采用周期性饥饿再投喂的方法,研究周期性饥饿再投喂对建鲤(1.5±0.2g)的生长、体组成、消化酶的影响。试验分为10个组,共进行60d。结果表明,在相同的饥饿天数下,延长再投喂天数可以使建鲤的增重率逐渐增加,而且S1F4、S2F8、S4F16组的增重率与对照组无显著性差异(P>0.05),9个周期性饥饿再投喂组在摄食期间的摄食率和特定生长率均显著性高于对照组(P<0.05),而且饵料系数均低于对照组,说明本试验建鲤的补偿生长是通过提高摄食率和饵料利用率共同实现的;周期性饥饿再投喂对全鱼水分、粗蛋白、粗灰分影响差异不显著(P>0.05),但9个周期性饥饿再投喂组的全鱼粗脂肪含量均低于对照组,而且在相同的饥饿天数下,随着再投喂天数的延长而升高;9个周期性饥饿再投喂组的肠道消化酶(蛋白酶、脂肪酶、淀粉酶)活力均高于对照组。

裂壶藻对津新鲤(Cyprinus carpio var.Jian)生长、血液非特异性免疫指标及抗病力的影响

选用初始体重为(26.77±1.56)g的津新鲤(Cyprinus carpio var.Jian)540尾,随机分为6组,每组3个重复,分别投喂含0、0.40%、0.80%、1.20%、1.60%和2.00%裂壶藻的等氮等能饲料,饲养56 d,探讨饲料中不同水平的裂壶藻对津新鲤生长、血液非特异性免疫指标及抗病力的影响。结果显示,在0.80%裂壶藻水平组中,增重率和特定生长率均显著提高(P<0.05),肥满度则在1.20%水平组中达到最大值(P<0.05),各组间成活率差异不显著(P>0.05)。不同水平的裂壶藻均可以显著提高津新鲤的抗氧化能力,其中过氧化氢酶、总超氧化物歧化酶和谷胱甘肽的含量均呈先上升后下降的趋势,且分别在1.20%、0.80%和1.20%组中达到最高(P<0.05);而丙二醛含量呈先下降后上升的趋势,在0.80%组中达最低(P<0.05)。不同水平的裂壶藻均可以显著提高津新鲤的非特异性免疫力,溶菌酶呈先上升后下降的趋势,其中1.20%组活性最高(P<0.05)。一氧化氮含量随裂壶藻水平增加而增加,各添加组含量均显著高于对照组(P<0.05)。谷草转氨酶/谷丙转氨酶在0.80%组中达到最小值(P<0.05)。随着裂壶藻水平的增加,津新鲤血细胞呼吸爆发活性和免疫保护率得到显著提高,感染嗜水气单胞菌后的累计死亡率则显著降低(P<0.05),其中,以0.80%组最佳。综合生长、血液非特异性免疫指标和抗病力来分析,饲料中裂壶藻的适宜添加水平为0.80%–1.20%。

不同脂肪源饲料对津新鲤(Cyprinus carpio var.Jian)生长及脂类代谢的影响

试验选用初始体重为(44.34±1.58)g的津新鲤1 260尾,随机分为6组,每组3个重复,每个重复70尾鱼。6组饲料分别含有豆油、鸡油、玉米油、棕榈油、菜籽油、棉籽油。饲养8周后探讨饲料中不同脂肪源对津新鲤生长、相关脂代谢酶活性及脂肪酸合成酶(FAS)、脂蛋白脂酶(LPL)基因表达的影响。结果表明:玉米油组的相对增重率、特定生长率及蛋白质效率显著高于其它5组(P<0.05),但玉米油组饵料系数则最低。不同脂肪源饲料对肝胰脏及肾脏中LPL、肝脂酶(HL)、总脂酶(GE)、脂肪代谢酶活性影响很大(P<0.05),且玉米油组酶活性均最高;各组肝胰脏及肾脏FAS变化不大(P>0.05)。从基因表达来看,玉米油组更容易诱导和调节肝胰脏和肾脏中FAS及LPL m RNA的表达(P<0.05),再次投喂后6 h,肝胰脏和肾脏中LPL m RNA表达丰度均达到峰值,而肝胰脏中FASm RNA表达丰度达到最低值,肾脏中FAS m RNA表达丰度逐渐下降。总之,玉米油可作为津新鲤优质脂肪源。

雌性长翅羊角蚱Criotettix longipennis Liang的首次发现及记述(直翅目:蚱总科)

记述首次发现的长翅羊角蚱Criotettix longipennisLiang雌性．雌性的构造和体色与雄性基本相同，但体型较大，体长：14．0～14．5mm；前胸背板长：16．0～16．5mm；后足股节长：8．8～9．0m／n．该标本保存在河池学院动物标本室和陕西师范大学动物研究所标本室．

黑龙江野鲤(Cyprinus haematopteru)与建鲤(Cyprinus carpio var.Jian)正反交与自交子代消化酶活力及其分布比较分析

在网箱养殖45天后,采用比较分析黑龙江野鲤与建鲤正反交与自交子代消化酶活力的方法,研究由杂交引起的遗传背景改变对消化酶活力及其分布的影响。结果表明,两杂交群体肝胰腺蛋白酶活力显著高于建鲤自交子代,后肠则显著低于后者,后肠脂肪酶活力显著高于黑龙江野鲤自交子代;黑龙江野鲤♂×建鲤♀子代各消化器官淀粉酶活力均显著高于两自交群体。两杂交群体除前、中肠淀粉酶活力差异显著度与建鲤自交子代不同外,3种消化酶活力分布及不同器官间的差异程度和两自交群体均相同;四个群体脂肪酶和淀粉酶活力在前、中肠及肝胰腺均表现出互补现象,后肠均有较高活力不互补。

Genetic mapping and QTL analysis for body weight in Jian carp (Cyprinus carpio var.Jian) compared with mirror carp (Cyprinus carpio L.)

We report the genetic linkage map of Jian carp(C yprinus carpio var. Jian). An F1 population comprising 94 Jian carp individuals was mapped using 254 microsatellite markers. The genetic map spanned 1 381.592 c M and comprised 44 linkage groups,with an average marker distance of 6.58 c M. We identified eight quantitative trait loci(QTLs) for body weight(BW) in seven linkage groups,explaining 12.6% to 17.3% of the phenotypic variance. Comparative mapping was performed between Jian carp and mirror carp( Cyprinus carpio L.),which both have 50 chromosomes. One hundred and ninety-eight Jian carp marker loci were found in common with the mirror carp map,with 186(93.94%) showing synteny. All 44 Jian carp linkage groups could be one-to-one aligned to the 44 mirror carp linkage groups,mostly sharing two or more common loci. Three QTLs for BW in Jian carp were conserved in mirror carp. QTL comparison suggested that the QTL confidence interval in mirror carp was more precise than the homologous interval in Jian carp,which was contained within the QTL interval in Jian carp. The syntenic relationship and consensus QTLs between the two varieties provide a foundation for genomic research and genetic breeding in common carp.

Nd-Sr Isotopic Geochemistry of the Late Archean-Paleoproterozoic Granitoids in the Lüliang-Wutai Terrain,North China Craton,and Implications for Petrogenesis

In this paper we report geochemical and Nd-Sr isotopic data for a late Archean gneissic granitic pluton （Hengling pluton）, an early Paleoproterozoic complex （Xipan complex） and a late Paleoproterozoic granitic pluton （Yunzhongshan granites） from the Liiliang-Wutai terrain, North China, to trace the source of these late Archean-Paleoproterozoic granitoids and, particularly, to understand the nature and mechanism of continental growth at that time. The Hengling granitic gneisses （ca. 2.51 Ga） are characterized by high Na2O and LILEs, TTG-like REE patterns （highly depleted HREE and minor Eu anomalies） and moderately depleted Nd-Sr isotopic compositions （εNd（t） =1.2-2.7, ISr=0.7015-0.7019）, and were considered as being products of arc magmatism that was developed upon the North China craton. The Xipan complex （ca. 2.2 Ga） contain gabbroic diorite and monzonite, mostly being Na2O-rich, highly fractionated REE patterns and isotopically enriched （εNd（t） =-1.5 to -4.1, Isr=0.7038-0.706）. The gabbroic diorites probably originated from melting of an enriched mantle source, but significantly contaminated by lower crustal material, and the monzonites probably represent a product of a mixture between the gabbroic dioritic magma and granitic melts of crustal origin. The Yunzhongshan post-collisional granitoids （ca. 1.8 Ga） are characterized by high-K affinity and highly-enriched and homogeneous Nd isotopic compositions （εNd（t）=-4.9 to -5.7）, although they split into two groups in terms of REE patterns： one group showing elevated HREE （and Sc, Y and Zr） with significant negative Eu anomalies and the other showing highly depleted HREE and, to a lesser extent, mid-REE with negligible Eu anomalies. These granites are genetically related to a process of extensional collapse of a thickened orogen. They formed through magma mixing between mantle-derived basaltic magmas and crust-derived granitic melts, followed by significant fractionation of ferromagnesian phases （like hornblende and Cpx） and feldspar and accessory zircons. Some Yunzhongshan granites show very old Nd model ages （2.9-3.0 Ga）, suggesting the existence of continental crust older than 2.7 Ga, which is supported by our zircon Hf isotopic data for these granites.

Efficacy of Jian’ganle(健肝乐) versus Hugan Pian(护肝片),Glucuronolactone and Reduced Glutathione in Prevention of Antituberculosis Drug-induced Liver Injury

Summary： Evidence-based medicine is advocated by WHO and adopted by developed countries for many years. In China, however, the selection of essential medicine and various medical insurance reimbursement schemes medicine is usually based on experts＇ experience of prescription practice which is under heavy critics resulting from the lack of related comparative efficacy and evidence-based research. The efficacy of Jian＇ganle in prevention of drug-induced liver injury （DILI） caused by antituberculotics was evaluated in this study by comparison with Hugan Pian, glucuronolactone and reduced glutathione. Evidence was provided for relevant sectors such as Ministry for Human Resources and Social Security of the People＇s Republic of China and National Health and Family Planning Commission of the Peo- ple＇s Republic of China to select and renew the Essential Medicine List （EML）, the new rural cooperative medical scheme in China （NRCMS） list or the reimbursement list of industrial injury insurance. A total of 189 patients with initial pulmonary tuberculosis were divided into four groups who took antituberculotics combined with Jian＇ganle, Hugan Pian, glucuronolactone and reduced glutathione respectively. Their liver function profile including alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, total bilirubin （TBIL）, direct bilirubin （DBIL）, total protein （TP）, albumin （A） and globulin （G） were detected at admission as baseline and after treatment. The Jian＇ganle group was compared with the three others by chi-square tests. In an aspect of maintaining bilirubin indexes normal, Jian＇ganle was more efficacious than glucuronolactone. And Jian＇ganle had a little more efficacy than reduced glutathione to maintain protein indexes normal as well. And the therapeutic regimen of antituberculotics combined with Jian＇ganle was the best in treating tuberculosis and preventing DILI at the same time. The study showed that among the four hepatinicas which demonstrated similar prevention of DILI caused by antituberculotics, Jian＇ganle has more advantages over the three others to some extent, which provides a reliable basis for health sectors to select and renew the EML, NRCMS List or the reimbursement list of industrial injury insurance.

Systems pharmacology approach reveals protective mechanisms of Jian-Pi Qing-Chang decoction on ulcerative colitis

BACKGROUND Given the complex pathogenesis of ulcerative colitis (UC), the conventional therapeutic methods are not fully curative. As a sort of systematic complementary and alternative medicine, traditional Chinese medicine (TCM) provides new options for the standard therapy. Nevertheless, there are still numerous problems with the promotion of TCM attributed to its complexity, and consequently, new research approaches are urgently needed. Thus, we explored the protective effects of Jian-Pi Qing-Chang (JPQC) decoction on UC based on systems pharmacology approach, which might fill the current innovation gap in drug discovery and clinical practice pertaining to TCM. AIM To investigate the protective mechanisms of JPQC decoction on UC based on systems pharmacology approach. METHODS We performed systems pharmacology to predict the active ingredients, the matched targets, and the potential pharmacological mechanism of JPQC on UC. In vivo, we explored the effects of JPQC in a colitis model induced by dextran sulfate sodium. In vitro, we adopted the bone marrow-derived macrophages (BMDMs) as well as BMDMs co-cultured with Caco2 cells to verify the underlying mechanisms and effects of JPQC on UC under TNF-α stimulation. RESULTS Systems pharmacology revealed 170 targets for the 107 active ingredients of JPQC and 112 candidate targets of UC. Protein-protein interaction networks were established to identify the underlying therapeutic targets of JPQC on UC. Based on enrichment analyses, we proposed our hypothesis that JPQC might have a protective effect on UC via the NF-κB/HIF-1α signalling pathway. Subsequent experimental validation revealed that treatment with TNFα activated the NF-κB/HIF-1α signalling pathway in BMDMs, thereby damaging the epithelial barrier permeability in co-cultured Caco2 cells, while JPQC rescued this situation. The findings were also confirmed in a dextran sulfate sodium-induced colitis model. CONCLUSION JPQC could improve the mucosal inflammatory response and intestinal epithelial barrier function via the NF-κB/HIF-1α signalling pathway, which provides new perspectives on the pharmaceutical development and clinical practice of TCM.

《现场JIAN》:打造广播融媒体传播品牌

融媒体时代,广播要提升传播力和影响力,应由传统的单向传播向多媒体传播转变。无锡广播电视台梁溪之声广播频率打造集广播、微信、微博、今日头条、视频直播“五位一体”的融媒体品牌栏目《现场JIAN》,提升主流媒体的传播力和影响力。

Analysis and Comment on“ Freedom”——Based on Liang Qichao's On Freedom

Liang Qichao 's analysis of "freedom "in On Freedom is mainly focused on revealing the evolution and variation of the term "freedom "in modern Chinese society. In his view,freedom is not only an indispensable element of an individual's life,but also fully applicable to the course of social and historical developm ent. Liang Qichao 's liberalism originates directly from the West,but is deeply influenced by the Chinese traditional discourse system and the overwhelming reality context of saving the nation in modern tim es.What is different from Western liberalism is that the dim ensions of Liang Qichao 's freedom are developed at two levels,one is the individual level,the other is the national level. The form er has the significance of enlightenm ent,while the latter has the significance of saving lives. Enlightenm ent and salvation are not only the"double variations"of modern C hina,but also the dual responsibility of modern Chinese enlightenm ent thinkers,which is dem-onstrated in Liang Qichao 's thought of freedom.

Calculating and Evaluating the Groundwater Resource of Jian San Jiang Area

According to the method of water balance,the parameters of groundwater resouce of Jian San Jiang area have been calculated in the paper.At the same time,the quality of water supplying and water mining can be calculated.Furthermore,the groundwater resource have been evaluated.Thus,the paper provides the important references for managers to using groundwater reasonable.

Kong Liang

Kong Liang is a young painter in the contemporary painting arena. He was born in the 1980s, among the so-called 'Post-1980' generation. Yet he and his paintings possess many characteristics different from other 'Post-1980'painters. In the heterogeneous and diverse

STUDY OF THE RELATIONSHIP BETWEEN THE HEPATOTOXICITY AND FREE RADICAL INDUCED BY 1,1,2-TRICHLOROETHANE AND 1,1,1-TRICHLOROETHANE IN RAT LIANG-FU XIA,DEPARTMENT OF TOXICOLOGY,SECOND MILITARY MEDICAL UNIVERSITY,SHANGHAI,CHINA.

1,1,2-trichloroethane(1,1,2-TCE) and 1,1,1-trichloro-ethane(1,1,1-TCE) were administered to rats to evaluatethe toxic effects on the rat liver.During whole animalstest the serum enzymes were assayed at 8 hr,12 hr,24 hr,36 hr,48 hr and 72 hr after the rat receiving 1,1,2-TCEand 1,1,1-TCE.The results showed that 1,1,2-TCE let tomuch higher values for SGPT,SSDH and SGDH than 1,1,1-TCEdid,especially at 24 hr after administration.At

Shu-Gan-Liang-Xue Decoction Simultaneously Down-regulates Expressions of Aromatase and Steroid Sulfatase in Estrogen Receptor Positive Breast Cancer Cells

Objective：Estradiol （E2） plays an important role in the development of breast cancer.In postmenopausal women,the estrogen can be synthesized via aromatase （CYP19） pathway and steroid-sulfatase （STS） pathway in peripheral tissues,when the production in ovary has ceased.The objective of our study was to explore the effects of Shu-Gan-Liang-Xue Decoction （SGLXD） on the expressions of CYP19 and STS in estrogen receptor positive breast cancer MCF-7 and T47D cells.Methods：The effects of SGLXD on the cell viability of MCF-7 and T47D were analyzed by MTT assay.By quantitative real-time RT-PCR and Western blot,we evaluated the mRNA and protein expressions of CYP19 and STS in MCF-7 and T47D cells after SGLXD treatment.Results：By MTT assay,the cell viability rates of MCF-7 and T47D were significantly inhibited by SGLXD in a dose-dependent manner,the IC50 values were 40.07 mg/ml for MCF-7 cells and 25.62 mg/ml for T47D cells,respectively.As evidenced by real-time PCR and Western blot,the high concentrations of SGLXD significantly down-regulated the expressions of CYP19 and STS both in the transcript level and the protein level.Conclusion：The results suggest that SGLXD is a potential dual aromatase-sulfatase inhibitor by simultaneously down-regulating the expressions of CYP19 and STS in MCF-7 and T47D cells.

LIANG Yue: The Quiet Rooms

Duration:5 December 2013-7 January 2014,Daily 10AM-6PM Venue:ShanghART H-Space,Bldg 18,No.50 Moganshan Rd.,Shanghai Artists:LIANG YueOn 5 December 2013,LIANG Yue present her second solo exhibi-tion The Quiet Rooms at ShanghART H-Space.Since her first exhibition at ShanghART back in 2003,the

A Case Study of Liang Qichao’s Literary Creations and Translations From Localization Theory

Intercultures play a crucial role in localization of translation. They are the essential reference resources for those who are translators as well as writers in their literary translation or creation. Translators contribute to the extension of source culture and in target culture by means of intercultures to form new localized cultural paradigms, which help writers to improve their creations. Liang Qichao’s literary creation was influenced by the literary paradigms he translated. Through the translation, he borrowed new literary paradigms from foreign languages and converted them into more acceptable ones in his literature. The paper, from the perspectives of localization and intercultures by Anthony Pym, analyzes the characteristics and interactions between Liang Qichao’s literary translation and literary creation.

Geochronological and geochemical comparison of Precambrian meta-mafic volcanics in Zhongtiaoshan and Lüliangshan Regions in Shanxi, North China Craton

To unravel the geochemical heterogeneity and its origin in different terranes of North China,we conducted geochronological and geochemical analyses of the meta-mafic rocks from the Lüliang–Zhongtiao rift zone(Shanxi Province).LA-ICP-MS zircon U–Pb dating yielded mostly End-Neoarchean to Proterozoic ages for the basement rocks(Sushui Complex:2516±26 Ma;Metamafic rocks:2494±31 Ma),Jiangxian Group(~2213 Ma),Zhongtiao Group(2077±29 Ma),Jiehekou Group(1998±23 Ma),and Lüliang Group(2152±52 Ma).Petrographic characteristics show that the meta-mafic rocks from the Neoarchean–Paleoproterozoic Zhongtiaoshan(Sushui Complex)have similar geochemical characteristics to the overlying Jiangxian and Zhongtiao Groups.The Paleoproterozoic Lüliang andYejishan Group meta-mafic rocks from Lüliangshan also have similar geochemical characteristics but are geochemically different from similar-age rocks from Zhongtiaoshan.This shows that the late-stage rocks have a geochemical inheritance from the early-stage rocks in the same region and that the geochemical heterogeneity of rocks from different areas was originated from the inherited heterogeneity of the magma source.