目的 研究制备 nor A基因介导的金黄色葡萄球菌对氟喹诺酮类药物的耐药机制的 Dig- nor A基因探针。方法 采用聚合酶链反应 (PCR)制备 Dig- nor A基因探针。结果 PCR法制备 Dig- nor A基因探针简便易行 ,可在较短时间内获得大量的探...目的 研究制备 nor A基因介导的金黄色葡萄球菌对氟喹诺酮类药物的耐药机制的 Dig- nor A基因探针。方法 采用聚合酶链反应 (PCR)制备 Dig- nor A基因探针。结果 PCR法制备 Dig- nor A基因探针简便易行 ,可在较短时间内获得大量的探针 ,所得探针有较高的敏感性 ;Dig- nor A基因探针安全、易操作 ,标记探针可长期保存。结论 为进一步研究 nor展开更多
按金黄色葡萄球菌多重耐药转运蛋白NorA的编码序列设计引物,以金黄色葡萄球菌基因组DNA为模板,扩增出norA基因中1 155 bp cDNA片段,将所得片段与pMD18-T载体连接,转化到感受态大肠杆菌JM109中,成功地筛选到阳性克隆,其质粒测序结果与文...按金黄色葡萄球菌多重耐药转运蛋白NorA的编码序列设计引物,以金黄色葡萄球菌基因组DNA为模板,扩增出norA基因中1 155 bp cDNA片段,将所得片段与pMD18-T载体连接,转化到感受态大肠杆菌JM109中,成功地筛选到阳性克隆,其质粒测序结果与文献报道一致。从阳性克隆中提取质粒,经NdeⅠ和XhoⅠ酶切,回收1 155 bp目的片段,定向克隆到pET-28a(+)表达载体中,转化感受态大肠杆菌DH5α,提取质粒,再次转化到BL21(DE3)中,成功地筛选到阳性克隆。经IPTG诱导阳性菌,通过SDS-PAGE检测出norA基因的表达。展开更多
As one of the most well- known plays of Henrik Ibsen, A Doll's House's publishment and premiere in 1879 aroused great controversy on the ideas and beliefs reflected in this play. The female protagonist, Nora, ...As one of the most well- known plays of Henrik Ibsen, A Doll's House's publishment and premiere in 1879 aroused great controversy on the ideas and beliefs reflected in this play. The female protagonist, Nora, is especially the center of dispute.In Act II, a scene of Nora's dancing Tarantella is worth discussing. It can not only be understood as a way of relieving, but the two men who gives Nora much pressure as well. Tarantella is also a dance of desire which leads to her breakaway from the loveless marriage and her departure from the family.展开更多
A doll’s house tells us a story of a woman,who is treated as a doll-child by her father,then a doll-wife by her husband,eventually realizes her subordinate role at home,so she resolutely leaves the apparently perfect...A doll’s house tells us a story of a woman,who is treated as a doll-child by her father,then a doll-wife by her husband,eventually realizes her subordinate role at home,so she resolutely leaves the apparently perfect marriage and tries to seek out her own individuality.It questions the entire fabric of marital relationships,and indicts all the false values of the masculine society.展开更多
文摘目的 研究制备 nor A基因介导的金黄色葡萄球菌对氟喹诺酮类药物的耐药机制的 Dig- nor A基因探针。方法 采用聚合酶链反应 (PCR)制备 Dig- nor A基因探针。结果 PCR法制备 Dig- nor A基因探针简便易行 ,可在较短时间内获得大量的探针 ,所得探针有较高的敏感性 ;Dig- nor A基因探针安全、易操作 ,标记探针可长期保存。结论 为进一步研究 nor
文摘As one of the most well- known plays of Henrik Ibsen, A Doll's House's publishment and premiere in 1879 aroused great controversy on the ideas and beliefs reflected in this play. The female protagonist, Nora, is especially the center of dispute.In Act II, a scene of Nora's dancing Tarantella is worth discussing. It can not only be understood as a way of relieving, but the two men who gives Nora much pressure as well. Tarantella is also a dance of desire which leads to her breakaway from the loveless marriage and her departure from the family.
文摘A doll’s house tells us a story of a woman,who is treated as a doll-child by her father,then a doll-wife by her husband,eventually realizes her subordinate role at home,so she resolutely leaves the apparently perfect marriage and tries to seek out her own individuality.It questions the entire fabric of marital relationships,and indicts all the false values of the masculine society.
