[Objective] The research aimed to distinguish Chinese herb Qingjiao from its botanical origin plants by comparing different DNA sequences,so as to provide a molecular basis for origin identification and quality evalua...[Objective] The research aimed to distinguish Chinese herb Qingjiao from its botanical origin plants by comparing different DNA sequences,so as to provide a molecular basis for origin identification and quality evaluation.[Method] The cpDNA psbA-trnH and nrDNA ITS sequences of five Chinese herb Qingjiao plants,including Gentiana macrophylla pall.,Gentiana straminea Maxim.,Gentiana crassicaulis Duthie ex Burk.,Gentiana dahurica Fisch and Gentiana officinalis H.Smith,were amplified with PCR,and then sequenced by direct PCR sequencing method for homologous analysis.[Results] The length of cpDNA psbA-trnH of five plants was 316-318 bp;there were seven different haplotypes and seven variable sites;the GC content of the sequence was 21.2%;the phylogenetic clustering showed the same result as haplotype analysis.The length of nrDNA ITS sequence of five plants was 624-625 bp,there were five different haplotypes and 13 variable sites;the GC content of the sequence was 59.3%.The result of phylogenetic clustering suggested that G.dahurica and G.straminea,G.macrophylla and G.officinalis clustered together as sister clades,respectively.[Conclusion] The nucleotide differences of nrDNA ITS regions could be used for distinguishing botanical origin in Chinese herb Qingjiao.展开更多
Kuo,Yeh-Tzu founded Taiwan’s Sung Shan Tsu Huei Temple in 1970.She organized more than 200 worshipers as a group named“Taiwan Tsu Huei Temple Queen Mother of the West Delegation to China to Worship at the Ancestral ...Kuo,Yeh-Tzu founded Taiwan’s Sung Shan Tsu Huei Temple in 1970.She organized more than 200 worshipers as a group named“Taiwan Tsu Huei Temple Queen Mother of the West Delegation to China to Worship at the Ancestral Temples”in 1990.At that time,the temple building of the Queen Mother Palace in Huishan of Gansu Province was in disrepair,and Temple Master Kuo,Yeh-Tzu made a vow to rebuild it.Rebuilding the ancestral temple began in 1992 and was completed in 1994.It was the first case of a Taiwan temple financing the rebuilding of a far-away Queen Mother Palace with its own donations.In addition,Sung Shan Tsu Huei Temple celebrated its 45th anniversary and hosted Yiwei Yuanheng Lizhen Daluo Tiandi Qingjiao(Momentous and Fortuitous Heaven and Earth Prayer Ceremony)in 2015.This is the most important and the grandest blessing ceremony of Taoism,a rare event for Taoism locally and abroad during this century.Those sacred rituals were replete with unprecedented grand wishes to propagate the belief in Queen Mother of the West.Stopping at nothing,Queen Mother’s love never ceases.展开更多
基金Supported by Science Foundation for the Excellent Youth and Middle-aged Scholars in Qinghai University(2009-QY-19)~~
文摘[Objective] The research aimed to distinguish Chinese herb Qingjiao from its botanical origin plants by comparing different DNA sequences,so as to provide a molecular basis for origin identification and quality evaluation.[Method] The cpDNA psbA-trnH and nrDNA ITS sequences of five Chinese herb Qingjiao plants,including Gentiana macrophylla pall.,Gentiana straminea Maxim.,Gentiana crassicaulis Duthie ex Burk.,Gentiana dahurica Fisch and Gentiana officinalis H.Smith,were amplified with PCR,and then sequenced by direct PCR sequencing method for homologous analysis.[Results] The length of cpDNA psbA-trnH of five plants was 316-318 bp;there were seven different haplotypes and seven variable sites;the GC content of the sequence was 21.2%;the phylogenetic clustering showed the same result as haplotype analysis.The length of nrDNA ITS sequence of five plants was 624-625 bp,there were five different haplotypes and 13 variable sites;the GC content of the sequence was 59.3%.The result of phylogenetic clustering suggested that G.dahurica and G.straminea,G.macrophylla and G.officinalis clustered together as sister clades,respectively.[Conclusion] The nucleotide differences of nrDNA ITS regions could be used for distinguishing botanical origin in Chinese herb Qingjiao.
文摘Kuo,Yeh-Tzu founded Taiwan’s Sung Shan Tsu Huei Temple in 1970.She organized more than 200 worshipers as a group named“Taiwan Tsu Huei Temple Queen Mother of the West Delegation to China to Worship at the Ancestral Temples”in 1990.At that time,the temple building of the Queen Mother Palace in Huishan of Gansu Province was in disrepair,and Temple Master Kuo,Yeh-Tzu made a vow to rebuild it.Rebuilding the ancestral temple began in 1992 and was completed in 1994.It was the first case of a Taiwan temple financing the rebuilding of a far-away Queen Mother Palace with its own donations.In addition,Sung Shan Tsu Huei Temple celebrated its 45th anniversary and hosted Yiwei Yuanheng Lizhen Daluo Tiandi Qingjiao(Momentous and Fortuitous Heaven and Earth Prayer Ceremony)in 2015.This is the most important and the grandest blessing ceremony of Taoism,a rare event for Taoism locally and abroad during this century.Those sacred rituals were replete with unprecedented grand wishes to propagate the belief in Queen Mother of the West.Stopping at nothing,Queen Mother’s love never ceases.
