OBJECTIVE:To investigate the possible mechanism underlying the effect of the Lushi Runzao decoction(路氏润燥汤)on Sjogren's syndrome using network pharmacology and to verify the mechanisms via animal experiments.M...OBJECTIVE:To investigate the possible mechanism underlying the effect of the Lushi Runzao decoction(路氏润燥汤)on Sjogren's syndrome using network pharmacology and to verify the mechanisms via animal experiments.METHODS:Available biological data on each drug in the Lushi Runzao decoction were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,and the target proteins of Sjogren's syndrome were retrieved from the GeneCards database.Information regarding Sjogren's syndrome and the targets of the drugs were compared to obtain overlapping elements.This information was imported into the STRING platform to obtain a proteinprotein interaction network diagram,following which a“component-target”network diagram was constructed using screened drug components and target information via Cytoscape software.The database for annotation,visualization,and integrated discovery was used for Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathways analyses.Pathway information predicted by network pharmacology was verified using animal experiments.RESULTS:The Lushi Runzao decoction ameliorated Sjogren's syndrome mainly by influencing tumor necrosis factor as well as certain cytokines and chemokines.The decoction also influenced the interleukin-17 and advanced glycosylation end products(AGE)-receptor for AGE signaling pathways.CONCLUSION:The Lushi Runzao decoction ameliorates Sjogren's syndrome via multiple targets and multiple signaling pathways.Network pharmacology is useful for making a comprehensive prediction regarding the efficacy of the Lushi Runzao decoction,and this information may be helpful in clinical research.展开更多
目的观察自拟养血祛风汤治疗血虚风燥型慢性湿疹的临床效果。方法选择2016年6月~2017年7月就诊于中国中医科学院西苑医院皮肤科的62例慢性湿疹患者,按照随机数字表法分为两组,各31例。治疗组口服自拟养血祛风汤200 m L/次,2次/d,对照组...目的观察自拟养血祛风汤治疗血虚风燥型慢性湿疹的临床效果。方法选择2016年6月~2017年7月就诊于中国中医科学院西苑医院皮肤科的62例慢性湿疹患者,按照随机数字表法分为两组,各31例。治疗组口服自拟养血祛风汤200 m L/次,2次/d,对照组口服氯雷他定10 mg/d,两组患者均予丁酸氢化可的松乳膏外用,2次/d。观察并比较两组治疗前后主要症状积分,并评价疗效。结果两组治疗前主要症状积分(包括瘙痒程度、皮损面积、皮损形态)比较差异无统计学差异意义(P>0.05);治疗后,两组主要症状积分均较治疗前明显降低(P<0.05),且治疗组评分低于对照组,差异有统计学意义(P<0.05)。治疗后,治疗组总有效率高于对照组,差异有统计学意义(P<0.05)。结论自拟养血祛风汤治疗血虚风燥型慢性湿疹疗效显著,能够改善患者的生活质量和临床症状,值得临床推广。展开更多
基金the National Natural Science Foundation of China:Based on the Intestinal flora/SCFAs/FFA2 Approach to Explore the Mechanism of Lushi Runzao Decoction in Regulating Th17/Treg Immune Balance to treat Sjogren's Syndrome(No.82104837)。
文摘OBJECTIVE:To investigate the possible mechanism underlying the effect of the Lushi Runzao decoction(路氏润燥汤)on Sjogren's syndrome using network pharmacology and to verify the mechanisms via animal experiments.METHODS:Available biological data on each drug in the Lushi Runzao decoction were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,and the target proteins of Sjogren's syndrome were retrieved from the GeneCards database.Information regarding Sjogren's syndrome and the targets of the drugs were compared to obtain overlapping elements.This information was imported into the STRING platform to obtain a proteinprotein interaction network diagram,following which a“component-target”network diagram was constructed using screened drug components and target information via Cytoscape software.The database for annotation,visualization,and integrated discovery was used for Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathways analyses.Pathway information predicted by network pharmacology was verified using animal experiments.RESULTS:The Lushi Runzao decoction ameliorated Sjogren's syndrome mainly by influencing tumor necrosis factor as well as certain cytokines and chemokines.The decoction also influenced the interleukin-17 and advanced glycosylation end products(AGE)-receptor for AGE signaling pathways.CONCLUSION:The Lushi Runzao decoction ameliorates Sjogren's syndrome via multiple targets and multiple signaling pathways.Network pharmacology is useful for making a comprehensive prediction regarding the efficacy of the Lushi Runzao decoction,and this information may be helpful in clinical research.
文摘目的观察自拟养血祛风汤治疗血虚风燥型慢性湿疹的临床效果。方法选择2016年6月~2017年7月就诊于中国中医科学院西苑医院皮肤科的62例慢性湿疹患者,按照随机数字表法分为两组,各31例。治疗组口服自拟养血祛风汤200 m L/次,2次/d,对照组口服氯雷他定10 mg/d,两组患者均予丁酸氢化可的松乳膏外用,2次/d。观察并比较两组治疗前后主要症状积分,并评价疗效。结果两组治疗前主要症状积分(包括瘙痒程度、皮损面积、皮损形态)比较差异无统计学差异意义(P>0.05);治疗后,两组主要症状积分均较治疗前明显降低(P<0.05),且治疗组评分低于对照组,差异有统计学意义(P<0.05)。治疗后,治疗组总有效率高于对照组,差异有统计学意义(P<0.05)。结论自拟养血祛风汤治疗血虚风燥型慢性湿疹疗效显著,能够改善患者的生活质量和临床症状,值得临床推广。
