We examined suitability of arbuscular mycorrhizal fungi (AMF) associated with cool-season nonnative forages on reclaimed surface-mined land in southeast Ohio for establishment of native warm-season grasses. The goal o...We examined suitability of arbuscular mycorrhizal fungi (AMF) associated with cool-season nonnative forages on reclaimed surface-mined land in southeast Ohio for establishment of native warm-season grasses. The goal of establishing these grasses is to diversify a post-reclamation landscape that is incapable of supporting native forest species. A 16-week glasshouse study compared AMF from a 30-year reclaimed mine soil (WL) with AMF from native Ohio tallgrass prairie soil (CL). Four native grasses were examined from seedling through 16 weeks of growth. Comparisons were made between CL and WL AMF on colonized (+AMF) and non-colonized plants (–AMF) at three levels of soil phosphorus (P). Leaves were counted at 4 week intervals. Shoot and root biomass and percent AMF root colonization were measured at termination. We found no difference between WL and CL AMF. Added soil P did not reduce AMF colonization, but did reduce AMF efficacy. Big bluestem (Andropogon gerardii Vitman), Indiangrass (Sorghastrum nutans (L.) Nash), and tall dropseed (Sporobolus asper (Michx.) Kunth) benefited from AMF only at low soil P while slender wheatgrass (Elymus trachycaulus (Link) Gould ex Shinners) exhibited no benefit. Establishment of tallgrass prairie dominants big blue-stem and Indiangrass would be supported by the mine soil AMF. It appears that the non-native forage species have supported AMF equally functional as AMF from a regionally native tallgrass prairie. Tall dropseed and slender wheatgrass were found to be less dependent on AMF than big bluestem or Indiangrass and thus would be useful in areas with little or no AMF inoculum.展开更多
Background:Incorporation of legume species into native North American pastures is considered an effective method to increase native pasture productivity and improve the nutritive value of forage.This study evaluated t...Background:Incorporation of legume species into native North American pastures is considered an effective method to increase native pasture productivity and improve the nutritive value of forage.This study evaluated the effects of inclusion of purple prairie clover(PPC,Dalea purpurea Vent.),a native legume forage,with native coolseason grasses on the in vitro fermentation and in situ digestibility of mixed forages.Methods:Whole plant PPC and mixtures of cool-season grasses were harvested when the PPC reached the vegetative(VEG),full flower(FL)and seedpod(SP)stages,and were combined in ratios(DM basis)of 0:100,25:75,50:50,75:25 and 100:0 at each maturity.In vitro ruminal incubations using these mixtures were conducted for 48 h to determine gas production(GP),in vitro DM disappearance(IVDMD),total volatile fatty acids(VFA)and ammonia-N production.Mixtures of forages harvested when the PPC reached the FL stage and 50:50 mixture of forages harvested at VEG,FL and SP stages were incubated in the rumen of three heifers for 0,2,6,12,24,48,72 and 96 h to determine in situ degradabilities of DM,neutral detergent fibre(aNDF)and crude protein(CP).Results:Contents of aNDF and ADF increased(P<0.01),while CP decreased(P<0.001)as PPC matured.Concentrations of extractable condensed tannins in PPC ranked as FL>VEG>SP(P<0.05).Regardless of PPC proportions in the mixture,GP decreased(P<0.05)with increasing PPC maturity.Increasing PPC proportions linearly increased(P<0.001)GP,IVDMD and total VFA at VEG,but linearly decreased(P<0.001)them at SP.Irrespective of PPC maturity,ammonia-N production linearly increased(P<0.01)with increasing proportions of PPC and the concentration was higher(P<0.05)at VEG than at FL and SP stages.Increasing proportion of PPC at either maturity linearly increased(P<0.001)molar percentage of acetate(A)and branched-chain VFA,but linearly decreased(P<0.001)molar percentage of propionate(P),resulting in a linearly increase(P<0.001)in the A:P ratio.Increasing FL PPC in the mixture linearly and quadratically(P<0.01)increased a(soluble fraction),but linearly and quadratically decreased(P<0.01)b(potentially degradable fraction)for DM and aNDF,resulting in linear(P<0.05)and quadratic(P<0.01)increases in DM and aNDF maximum potential degradabilities(a+b).Effective degradabilities of DM and aNDF were also linearly and quadratically increased(P<0.05),and CP was quadratically increased(P<0.05)with increasing FL PPC,with the greatest effective degradability being observed with ratios between 50:50 and 75:25.Ruminal maximum potential degradabilities of DM and aNDF decreased(P<0.001)as the forage matured.Effective degradability of DM ranked as VEG>FL>SP(P<0.001),whereas the effective degradability of aNDF was similar between VEG and FL and both were greater(P<0.01)than SP.Conclusions:Inclusion of vegetative PPC in a mixed forage diet resulted in the greatest digestibility and incorporation of PPC before seedpod stage with native grasses had a positive effect on ruminal fermentation.Effects of PPC on ruminal digestion depend on both the stage of maturity and its proportion in mixed legume-grass pastures.Pastures containing 50%of PPC in full flower stage would likely provide the greatest quality diet to grazing ruminants subject to potential animal selectivity.展开更多
Soil compaction is a limitation to establishment of native forest species on reclaimed surfacemined lands in Appalachia. Previously, non-native forage species such as tall fescue (Schedonorus arundinaceus(Schreb.) Dum...Soil compaction is a limitation to establishment of native forest species on reclaimed surfacemined lands in Appalachia. Previously, non-native forage species such as tall fescue (Schedonorus arundinaceus(Schreb.) Dumort., nom. cons.) have been planted because they easily established on reclaimed mine soil. There is now interest in establishing robust native prairie species to enhance biodiversity and provide greater potential for root activity in the compacted soil. We conducted a 10-week glasshouse study comparing growth of “Pete” eastern gamagrass (Tripsacum dactyloidesL.), “Bison” big bluestem (Andropogon gerardiiVitman), and “Jesup MaxQ” tall fescue at soil bulk densities (BD) of 1.0, 1.3, and 1.5 g·cm-3. We also examined effects of arbuscular-mycorrhizal fungi (AMF) on plant growthin relation to compaction. Sources of AMF were a reclaimed surface coal mine soil and a native tallgrass prairie soil. Shoot and root biomass of tall fescue and big bluestem were reduced at 1.5 BD while eastern gamagrass growth was not affected. Growth ofbig bluestem and eastern gamagrass was greaterwith AMF than without, butsimilar between AMF sources. Tall fescue growthwas not enhanced by AMF. Overall, tall fescue biomass was 3 times greater than eastern gamagrass and 6 times greater than big bluestem when comparing only AMF-colonized grasses. Eastern gamagrass and big bluestem are both slower to establish than tall fescue. Eastern gamagrass appears to be more tolerant of compaction, while big bluestem appears somewhat less tolerant.展开更多
The aim of this research was to evaluate the salinity tolerance in prairie grass populations at the seedling stage quantifying the variability and the influence of physiological traits related to it. Salinity toleranc...The aim of this research was to evaluate the salinity tolerance in prairie grass populations at the seedling stage quantifying the variability and the influence of physiological traits related to it. Salinity tolerance, in </span><i><span style="font-family:Verdana;">Bromus</span></i> <i><span style="font-family:Verdana;">catharticus</span></i><span style="font-family:Verdana;"> Vahl (prairie grass) populations collected in different environments of the Pampean Phytogeography region (Argentine) was evaluated at the seedling stage, using controlled condition of temperature and light. It was adopted a completely randomized design using 3 plots with three plants each one per population and two levels of treatment: 0 mM and 100 mM NaCl. Morphological, biomass and membrane stability root and shoot traits were studied. A factorial ANOVA with interaction was estimated. Then one way ANOVA for all seedling traits in both treatments allowed estimating variance components, coefficient of genotypic determination (CGD) and variation index (VI). Comparisons between populations were made using Tukey test (at 5% of probability). Phenotypic correlations among traits were calculated and then a path coefficient analysis separated direct and indirect effects at 100 and 0 mM NaCl. No significant interactions “Population × Treatment” were found for any character. The saline stress caused a pairing in the population means for the most traits. Coefficients of variation were mainly higher when the seedlings grew without stress (0 mM) because it allowed a greater potential genotypic expression. The absence of significant interactions denotes a good homeostatic capacity of the prairie grass facing that abiotic stress. Leaf length, shoot length and root dry matter were the variables with the largest direct and indirect effects. Our results showed an increase for them at salt and demonstrated intraspecific variation, possibly in relation with the origin sites. Plants under stress showed a marked resilience, in order to quickly restore the same biomass allocation patterns that occur in non-stress environment.展开更多
The study focus was an examination of the hydrothermal pretreatment method applied to the lignocellulosic substrate, represented by the prairie cord grass, and comparison between different conditions based on the yiel...The study focus was an examination of the hydrothermal pretreatment method applied to the lignocellulosic substrate, represented by the prairie cord grass, and comparison between different conditions based on the yield of glucose after enzymatic hydrolysis. The treatment did not involve any chemicals usage. Enzymatic hydrolysis was performed in order to examine the amount of glucose which was released from pretreated materials. The most efficient pretreatment conditions were at high temperature and relatively short reaction time (210°C and 10 min), after which the lignocellulose structure was the most available for enzymes actions which resulted in a pretreatment conversion rate of 97%. Temperature had a significant influence on glucose release during the hydrolysis, which was confirmed by the Michaelis-Menten and kinetic models. Kinetic models were used to fit the inhibitors and their conversion rates were related to temperature.展开更多
文摘We examined suitability of arbuscular mycorrhizal fungi (AMF) associated with cool-season nonnative forages on reclaimed surface-mined land in southeast Ohio for establishment of native warm-season grasses. The goal of establishing these grasses is to diversify a post-reclamation landscape that is incapable of supporting native forest species. A 16-week glasshouse study compared AMF from a 30-year reclaimed mine soil (WL) with AMF from native Ohio tallgrass prairie soil (CL). Four native grasses were examined from seedling through 16 weeks of growth. Comparisons were made between CL and WL AMF on colonized (+AMF) and non-colonized plants (–AMF) at three levels of soil phosphorus (P). Leaves were counted at 4 week intervals. Shoot and root biomass and percent AMF root colonization were measured at termination. We found no difference between WL and CL AMF. Added soil P did not reduce AMF colonization, but did reduce AMF efficacy. Big bluestem (Andropogon gerardii Vitman), Indiangrass (Sorghastrum nutans (L.) Nash), and tall dropseed (Sporobolus asper (Michx.) Kunth) benefited from AMF only at low soil P while slender wheatgrass (Elymus trachycaulus (Link) Gould ex Shinners) exhibited no benefit. Establishment of tallgrass prairie dominants big blue-stem and Indiangrass would be supported by the mine soil AMF. It appears that the non-native forage species have supported AMF equally functional as AMF from a regionally native tallgrass prairie. Tall dropseed and slender wheatgrass were found to be less dependent on AMF than big bluestem or Indiangrass and thus would be useful in areas with little or no AMF inoculum.
基金This study was funded from AAFC/Canadian Beef Cattle Industry Science ClusterAlberta Livestock and Meat Agency.This is Lethbridge Research and Development Centre contribution number 38717038.
文摘Background:Incorporation of legume species into native North American pastures is considered an effective method to increase native pasture productivity and improve the nutritive value of forage.This study evaluated the effects of inclusion of purple prairie clover(PPC,Dalea purpurea Vent.),a native legume forage,with native coolseason grasses on the in vitro fermentation and in situ digestibility of mixed forages.Methods:Whole plant PPC and mixtures of cool-season grasses were harvested when the PPC reached the vegetative(VEG),full flower(FL)and seedpod(SP)stages,and were combined in ratios(DM basis)of 0:100,25:75,50:50,75:25 and 100:0 at each maturity.In vitro ruminal incubations using these mixtures were conducted for 48 h to determine gas production(GP),in vitro DM disappearance(IVDMD),total volatile fatty acids(VFA)and ammonia-N production.Mixtures of forages harvested when the PPC reached the FL stage and 50:50 mixture of forages harvested at VEG,FL and SP stages were incubated in the rumen of three heifers for 0,2,6,12,24,48,72 and 96 h to determine in situ degradabilities of DM,neutral detergent fibre(aNDF)and crude protein(CP).Results:Contents of aNDF and ADF increased(P<0.01),while CP decreased(P<0.001)as PPC matured.Concentrations of extractable condensed tannins in PPC ranked as FL>VEG>SP(P<0.05).Regardless of PPC proportions in the mixture,GP decreased(P<0.05)with increasing PPC maturity.Increasing PPC proportions linearly increased(P<0.001)GP,IVDMD and total VFA at VEG,but linearly decreased(P<0.001)them at SP.Irrespective of PPC maturity,ammonia-N production linearly increased(P<0.01)with increasing proportions of PPC and the concentration was higher(P<0.05)at VEG than at FL and SP stages.Increasing proportion of PPC at either maturity linearly increased(P<0.001)molar percentage of acetate(A)and branched-chain VFA,but linearly decreased(P<0.001)molar percentage of propionate(P),resulting in a linearly increase(P<0.001)in the A:P ratio.Increasing FL PPC in the mixture linearly and quadratically(P<0.01)increased a(soluble fraction),but linearly and quadratically decreased(P<0.01)b(potentially degradable fraction)for DM and aNDF,resulting in linear(P<0.05)and quadratic(P<0.01)increases in DM and aNDF maximum potential degradabilities(a+b).Effective degradabilities of DM and aNDF were also linearly and quadratically increased(P<0.05),and CP was quadratically increased(P<0.05)with increasing FL PPC,with the greatest effective degradability being observed with ratios between 50:50 and 75:25.Ruminal maximum potential degradabilities of DM and aNDF decreased(P<0.001)as the forage matured.Effective degradability of DM ranked as VEG>FL>SP(P<0.001),whereas the effective degradability of aNDF was similar between VEG and FL and both were greater(P<0.01)than SP.Conclusions:Inclusion of vegetative PPC in a mixed forage diet resulted in the greatest digestibility and incorporation of PPC before seedpod stage with native grasses had a positive effect on ruminal fermentation.Effects of PPC on ruminal digestion depend on both the stage of maturity and its proportion in mixed legume-grass pastures.Pastures containing 50%of PPC in full flower stage would likely provide the greatest quality diet to grazing ruminants subject to potential animal selectivity.
文摘Soil compaction is a limitation to establishment of native forest species on reclaimed surfacemined lands in Appalachia. Previously, non-native forage species such as tall fescue (Schedonorus arundinaceus(Schreb.) Dumort., nom. cons.) have been planted because they easily established on reclaimed mine soil. There is now interest in establishing robust native prairie species to enhance biodiversity and provide greater potential for root activity in the compacted soil. We conducted a 10-week glasshouse study comparing growth of “Pete” eastern gamagrass (Tripsacum dactyloidesL.), “Bison” big bluestem (Andropogon gerardiiVitman), and “Jesup MaxQ” tall fescue at soil bulk densities (BD) of 1.0, 1.3, and 1.5 g·cm-3. We also examined effects of arbuscular-mycorrhizal fungi (AMF) on plant growthin relation to compaction. Sources of AMF were a reclaimed surface coal mine soil and a native tallgrass prairie soil. Shoot and root biomass of tall fescue and big bluestem were reduced at 1.5 BD while eastern gamagrass growth was not affected. Growth ofbig bluestem and eastern gamagrass was greaterwith AMF than without, butsimilar between AMF sources. Tall fescue growthwas not enhanced by AMF. Overall, tall fescue biomass was 3 times greater than eastern gamagrass and 6 times greater than big bluestem when comparing only AMF-colonized grasses. Eastern gamagrass and big bluestem are both slower to establish than tall fescue. Eastern gamagrass appears to be more tolerant of compaction, while big bluestem appears somewhat less tolerant.
文摘The aim of this research was to evaluate the salinity tolerance in prairie grass populations at the seedling stage quantifying the variability and the influence of physiological traits related to it. Salinity tolerance, in </span><i><span style="font-family:Verdana;">Bromus</span></i> <i><span style="font-family:Verdana;">catharticus</span></i><span style="font-family:Verdana;"> Vahl (prairie grass) populations collected in different environments of the Pampean Phytogeography region (Argentine) was evaluated at the seedling stage, using controlled condition of temperature and light. It was adopted a completely randomized design using 3 plots with three plants each one per population and two levels of treatment: 0 mM and 100 mM NaCl. Morphological, biomass and membrane stability root and shoot traits were studied. A factorial ANOVA with interaction was estimated. Then one way ANOVA for all seedling traits in both treatments allowed estimating variance components, coefficient of genotypic determination (CGD) and variation index (VI). Comparisons between populations were made using Tukey test (at 5% of probability). Phenotypic correlations among traits were calculated and then a path coefficient analysis separated direct and indirect effects at 100 and 0 mM NaCl. No significant interactions “Population × Treatment” were found for any character. The saline stress caused a pairing in the population means for the most traits. Coefficients of variation were mainly higher when the seedlings grew without stress (0 mM) because it allowed a greater potential genotypic expression. The absence of significant interactions denotes a good homeostatic capacity of the prairie grass facing that abiotic stress. Leaf length, shoot length and root dry matter were the variables with the largest direct and indirect effects. Our results showed an increase for them at salt and demonstrated intraspecific variation, possibly in relation with the origin sites. Plants under stress showed a marked resilience, in order to quickly restore the same biomass allocation patterns that occur in non-stress environment.
文摘The study focus was an examination of the hydrothermal pretreatment method applied to the lignocellulosic substrate, represented by the prairie cord grass, and comparison between different conditions based on the yield of glucose after enzymatic hydrolysis. The treatment did not involve any chemicals usage. Enzymatic hydrolysis was performed in order to examine the amount of glucose which was released from pretreated materials. The most efficient pretreatment conditions were at high temperature and relatively short reaction time (210°C and 10 min), after which the lignocellulose structure was the most available for enzymes actions which resulted in a pretreatment conversion rate of 97%. Temperature had a significant influence on glucose release during the hydrolysis, which was confirmed by the Michaelis-Menten and kinetic models. Kinetic models were used to fit the inhibitors and their conversion rates were related to temperature.
