To explore the mechanism of Yigong San anti-gastric cancer and immune regulation

BACKGROUND Yigong San(YGS)is a representative prescription for the treatment of digestive disorders,which has been used in clinic for more than 1000 years.However,the mechanism of its anti-gastric cancer and regulate immunity are still remains unclear.AIM To explore the mechanism of YGS anti-gastric cancer and immune regulation.METHODS Firstly,collect the active ingredients and targets of YGS,and the differentially expressed genes of gastric cancer.Secondly,constructed a protein-protein interaction network between the targets of drugs and diseases,and screened hub genes.Then the clinical relevance,mutation and repair,tumor microenvironment and drug sensitivity of the hub gene were analyzed.Finally,molecular docking was used to verify the binding ability of YGS active ingredient and hub genes.RESULTS Firstly,obtained 55 common targets of gastric cancer and YGS.The Kyoto Encyclopedia of Genes and Genomes screened the microtubule-associated protein kinase signaling axis as the key pathway and IL6,EGFR,MMP2,MMP9 and TGFB1 as the hub genes.The 5 hub genes were involved in gastric carcinogenesis,staging,typing and prognosis,and their mutations promote gastric cancer progression.Finally,molecular docking results confirmed that the components of YGS can effectively bind to therapeutic targets.CONCLUSION YGS has the effect of anti-gastric cancer and immune regulation.

Decadal Forecasts of Large Earthquakes along the Northern San Andreas Fault System, California: Increased Activity on Regional Creeping Faults Prior to Major and Great Events

The three largest earthquakes in northern California since 1849 were preceded by increased decadal activity for moderate-size shocks along surrounding nearby faults. Increased seismicity, double-difference precise locations of earthquakes since 1968, geodetic data and fault offsets for the 1906 great shock are used to re-examine the timing and locations of possible future large earthquakes. The physical mechanisms of regional faults like the Calaveras, Hayward and Sargent, which exhibit creep, differ from those of the northern San Andreas, which is currently locked and is not creeping. Much decadal forerunning activity occurred on creeping faults. Moderate-size earthquakes along those faults became more frequent as stresses in the region increased in the latter part of the cycle of stress restoration for major and great earthquakes along the San Andreas. They may be useful for decadal forecasts. Yearly to decadal forecasts, however, are based on only a few major to great events. Activity along closer faults like that in the two years prior to the 1989 Loma Prieta shock needs to be examined for possible yearly forerunning changes to large plate boundary earthquakes. Geodetic observations are needed to focus on identifying creeping faults close to the San Andreas. The distribution of moderate-size earthquakes increased significantly since 1990 along the Hayward fault but not adjacent to the San Andreas fault to the south of San Francisco compared to what took place in the decades prior to the three major historic earthquakes in the region. It is now clear from a re-examination of the 1989 mainshock that the increased level of moderate-size shocks in the one to two preceding decades occurred on nearby East Bay faults. Double-difference locations of small earthquakes provide structural information about faults in the region, especially their depths. The northern San Andreas fault is divided into several strongly coupled segments based on differences in seismicity.

融合多尺度通道注意力的开放词汇语义分割模型SAN

随着视觉语言模型的发展,开放词汇方法在识别带注释的标签空间之外的类别方面具有广泛应用。相比于弱监督和零样本方法,开放词汇方法被证明更加通用和有效。文章研究的目标是改进面向开放词汇分割的轻量化模型SAN,即引入基于多尺度通道注意力的特征融合机制AFF来改进该模型,并改进原始SAN结构中的双分支特征融合方法。然后在多个语义分割基准上评估了该改进算法,结果显示在几乎不改变参数量的情况下,模型表现有所提升。这一改进方案有助于简化未来开放词汇语义分割的研究。

SAN单元设备布置探讨

设备布置是化工工程设计的核心,也是开始详细工程设计的重要前提。设备布置既要做到安全、经济合理,也需满足工艺设计及操作、检维修和施工的要求。本文针对ABS装置中SAN单元部分展开设备布置讨论。

Combination of Shengmai San and Radix puerariae ameliorates depression-like symptoms in diabetic rats at the nexus of PI3K/BDNF/SYN protein expression

Background:Hyperglycemia is a characteristic feature of diabetes that often results in neuropsychological complications such as depression.Diabetic individuals are more vulnerable to experience depression compared to the normal population.Thus,novel treatment approaches are required to reduce depressive symptoms among diabetic individuals.Traditional Chinese medicines(TCMs)such as Shengmai San(SMS)and Radix puerariae(R)are usually widely used to treat ailments such as neurological com-plications since ancient time.Methods:In this study,SMS was combined with R to prepare an R-SMS formulation and screened for their antidepressant activity in diabetic rats.The antidepressant po-tential of the prepared combination was evaluated behaviorally using open field test,novelty-induced hypophagia,and forced swim test in diabetic rats with biochemical and protein expression(PI3K,BDNF[brain-derived neurotrophic factor],and SYN[pr-esynaptic vesicle protein])analysis.Results:Diabetic rats(streptozotocin,45 mg/kg)showed elevated fasting blood glu-cose(FBG)>12 mM with depressive symptoms throughout the study.Treatment with R-SMS(0.5,1.5,and 4.5 g/kg)significantly reverted depressive symptoms in diabetic rats as evinced by significantly(p<0.05)reduced immobility time with an increased tendency to eat food in a novel environment.Treatment with R-SMS also significantly increased the protein expression of PI3K,BDNF,and SYN protein,which play a crucial role in depression.Conclusion:This study showed that R-SMS formulation antagonized depressive symptoms in diabetic rats;thus,this formulation might be studied further to develop as an antidepressant.

SAN反应系统中单体竞聚率的测定及应用的研究

通过对SAN反应釜中苯乙烯、丙烯腈瞬时组成与聚合物中粒子的组分之间的关系进行研究,实验测定SAN反应釜在固定转化率下不同单体组成形成共聚物组分,通过计算确定各单体在现有反应条件下的竞聚率,根据研究结果,修正反应釜内单体组成与聚合物组分函数曲线,并分析不同反应条件对所得曲线造成的影响,以达到新牌号SAN开发时反应釜进料组成制定及优化的目的。

Metabolomics characterization of Qingwei San on oral ulcer in db/db mice with stomach heat pattern

Objective:To characterize the effects of Qingwei San(QWS)on diabetic oral ulcer(OU)mice with stomach heat pattern through metabolomic analysis.Methods:A stomach heat pattern mouse model was established by treating C57BLKS/J Leprdb/db(db/db)mice with dried Zingiber officinale Rosc.rhizome(Z.officinale,Gan Jiang)decoction by gavage.All model mice had blood glucose levels of≥11.1 m M.Subsequently,OU was induced by Na OH cauterization.After 1 week of administration of QWS,non-targeted metabolomic analysis of serum was conducted using ultra high performance liquid chromatography coupled with mass spectrometry(UHPLC-MS/MS).Results:The non-targeted metabolomics results indicated that tryptophan metabolism,2-oxocarboxylic acid metabolism,serotonergic synapses,amino sugar and nucleotide sugar metabolism,and amino acid biosynthesis were involved in the therapeutic effects of QWS,with tryptophan metabolism playing a predominant role.Conclusion:QWS treatment can significantly improve the pathological status of diabetic OU mice with stomach heat pattern.QWS may regulate the release of inflammatory factors through the tryptophan metabolism pathway.

基于肝脏蛋白质组学探讨加味丹栀逍遥散的抗抑郁作用及机制

[目的]探讨加味丹栀逍遥散对慢性不可预知性温和应激(CUMS)抑郁小鼠模型的干预作用及可能机制.[方法]C57BL/6N小鼠分为正常组、模型组、加味丹栀逍遥散组和氟西汀组.采用CUMS复制抑郁模型,模型成功后灌胃给药4周.末次给药后进行行为学测试,采用串联质谱标签法(TMT)蛋白质组学分析小鼠肝脏差异蛋白的表达谱,通过Kyoto encyclopedia of genes and genomes(KEGG)通路富集加味丹栀逍遥散抗抑郁的相关通路和蛋白;血液生化检测总胆固醇及总胆汁酸含量.[结果]与正常组相比,模型组小鼠糖水偏好率下降,自主活动次数减少,加味丹栀逍遥散给药后上调小鼠糖水偏好率及自主活动次数.蛋白组学结果显示模型组与加味丹栀逍遥散给药组之间肝脏差异表达蛋白20个,其中上调11个,下调9个.KEGG通路富集分析发现差异表达蛋白主要参与了胆固醇代谢、初级胆汁酸的合成等.生化检测发现,与正常组相比,模型组总胆固醇含量下降而胆汁酸含量升高,加味丹栀逍遥散给药能显著增加总胆固醇含量而减少总胆汁酸含量.[结论]加味丹栀逍遥散具有良好的抗抑郁效应,其机制可能与调节肝脏胆固醇异常代谢、降低血液总胆汁酸有关.

SKALAR San++连续流动分析仪测定水中总氮的方法研究

利用SKALAR San++连续流动分析仪测定了水中的总氮含量,结果表明:此方法具有自动化程度高、分析速度快、试剂用量少等优点。该方法的线性范围为0~5.00 mg/L,线性相关系数达到0.99994,检出限为0.04 mg/L,加标回收率为90%~107%。对比传统行业标准分析法,连续流动分析法可实现全过程自动化处理、大大节省人力,并提高了检测效率,且两种方法的测定结果无显著性差异,而连续流动分析法更适合于大批量样品和复杂样品的批量检测与分析。

The active mechanism of the Sheng Yang San Huo decoction on diabetic peripheral neuropathy on network pharmacology

Objectives:To discuss the mechanism of Sheng Yang San Huo decoction on diabetic peripheral neuropathy using the network pharmacology method.Methods:The BATMAN-TCM database,TCM-ID database,Chinese Natural Product Chemical Composition Database,and TCMIP database were employed to screen the chemical active ingredients of each herb in Sheng Yang San Huo decoction based on the“Libinsky Drug Rules”.SwissTargetPrediction was used to screen effective action targets for each herb in the prescription.Additionally,Cytoscape 3.7.0 was utilized to construct a“drug-target”network.GeneCards,OMIM,and MaLaCards databases were utilized to gather targets related to diabetic peripheral neuropathy.VENNY 2.1 online platform was employed to match drug and disease targets,draw a Venn diagram,and construct a“drug-active compounds-common target”network using Cytoscape 3.7.0.gene ontology biological process analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis for the targets were conducted using the DAVID 6.8 database.Enrichment analysis results were visualized using the OmicShareTool online platform.Molecular docking was performed using CB-Dock2.Results:Following screening,a total of 217 active compounds and 132 potential targets were identified in Sheng Yang San Huo decoction.The effects are primarily enriched in pathways such as Lipid and Atherosclerosis,AGE-RAGE signaling pathway in diabetic complications,and the IL-17 signaling pathway.The binding energy of the key active ingredients to the core protein targets of DPN was favorable.Conclusion:The study reveals the characteristics of multiple targets and pathways of Sheng Yang San Huo decoction,providing new insights for the clinical application of this prescription.

精致与摩登——法国轻奢品牌SANDRO眼镜赏析

品牌故事SANDRO品牌由Evelyne Chetrite和Didier Chetrite夫妇于1984年在法国创立,并在法国巴黎圣殿老街开设了第一家精品店。迄今为止,SANDRO在39个国家拥有超过646个销售点,以典雅别致的法式风格著称,以中高价为定位掀起了追捧“Accessible Luxury”(轻奢)的热潮,不仅受到了都市年轻女性的钟爱,在不少明星街拍中也经常能见到身着SANDRO服饰或配饰的身影。

针刺关元-三阴交对DOR患者卵巢储备功能及卵巢血流灌注的影响

目的探究针刺关元-三阴交对卵巢储备功能下降(DOR)患者卵巢储备功能及卵巢血流灌注的影响。方法选取2021年1月至2022年12月我院收治的DOR患者80例进行研究。将80例患者均分为观察组和对照组各40例。对照组:基础治疗。观察组:基础治疗+针刺关元-三阴交。观察卵巢储备功能、卵巢血流灌注及不良反应。结果两组治疗前后的AFC比较差异均不显著(P>0.05),治疗前,两组FSH、FSH/LH、AMH差异不显著,P>0.05,治疗后的FSH、FSH/LH均降低,AMH增加,P<0.05,且观察组FSH(9.32±2.15)U/L、FSH/LH(1.81±0.21)低于对照组(10.77±2.22)U/L、(1.98±0.23),AMH(1.75±0.50)ng/mL高于对照组(1.51±0.54)ng/mL,差异显著,P<0.05。两组治疗前后的卵巢直径比较差异均不显著(P>0.05),治疗前,两组DOR患者PI、RI差异不显著,P>0.05,治疗后,PI、RI均降低,观察组PI(0.80±0.11)、RI(0.53±0.10)低于对照组(0.86±0.14)、(0.58±0.12),差异显著,P<0.05。两组均无血肿、感染、疼痛、晕针、滞针等不良反应。结论针刺关元-三阴交能够显著改善DOR患者卵巢储备功能及卵巢血流灌注,且安全性高。

芪红散对巨噬细胞分化及炎症水平的影响

目的 研究芪红散对巨噬细胞分化及炎症水平的影响及其机制。方法 将单核细胞在PKC激活剂(PMA)的诱导下转化为巨噬细胞。仅使用PMA诱导的细胞定义为M0组,使用脂多糖(LPS)和γ干扰素(IFN-γ)诱导处理的细胞定义为M1组,在使用LPS和IFN-γ诱导处理的基础上加入低剂量以及高剂量芪红散进行干预的两组分别定义为M1+芪红散-L组以及M1+芪红散-H组。分别采用细胞计数试剂-8(CCK-8)法、流式细胞术、酶联免疫吸附试验(ELISA)、免疫印迹试验(WB)检测4组细胞的增殖、凋亡、迁移情况和肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、p50、p-p65水平。结果 与M0对比,M1组增殖、迁移、CD86^(+)分化比例、TNF-α、IL-6、p50及p-p65水平显著增加(P均<0.05),CD206^(+)分化比例、IL-10水平显著降低(P均<0.05)。与M1组比较,M1+芪红散-L组与M1+芪红散-H组CD86^(+)分化比例、TNF-α、IL-6表达水平均降低(P<0.05),且M1+芪红散-H组TNF-α及IL-6表达水平明显低于M1+芪红散-L组(P<0.05),IL-10表达水平明显高于M1+芪红散-L组(P<0.05)。与M1组比较,M1+芪红散-H组p50及p-p65表达水平均降低(P<0.05),M1+芪红散-L组206^(+)分化显著升高(P<0.05)。结论 芪红散可有效地减少巨噬细胞的CD86^(+)型分化,抑制炎症信号通路核因子-κB(Nuclearfactor kappaB,NF-κB)的激活从而降低炎症因子的表达,减轻炎症反应。

从“三才”到“三纲”:董仲舒王道观之嬗变

董仲舒王道观由“三才”向“三纲”之嬗变,蕴含着由法天以为仁到本天以立仁(人)的思想。这同时意味着“元”机展露与开显。纵向层面,董子之天备具了本然性根基,从而使天哲学乃至“天人一”得以确立;横向维度,天地人以及物共“元”之性而成“天下一”。董子王道观于“元”的确认中从终极意义上证成了王道本正的先天依据,并由此建构了“天人一”与“天下一”统一的“王天下”图式。

三黄柴术方调控Nrf2抗氧化应激防治急性肝内胆汁淤积性肝损伤

目的探讨三黄柴术方对异硫氰萘酯(ANIT)诱导的急性肝内胆汁淤积大鼠肝脏Nrf2表达及氧化应激水平的影响。方法SD雄性大鼠48只,随机分为正常组、模型组、复方高、中、低剂量组及熊去氧胆酸(UDCA)组,每组8只。实验第3天使用一次性灌服4%ANIT 100 mg/kg造模,各药物干预组于第1~5天进行不同药物灌胃。造模后48 h取材。空腹麻醉后腹腔静脉抽血检测血清肝功能;分离肝脏,存入-80℃冰箱中待用,测定SOD活力、MDA含量、GSH-PX和CAT活力,fluorescent quantitative PCR测定肝组织Nrf2、SOD1、SOD2、SOD3、CAT的mRNA表达,WB检测肝组织Nrf2的蛋白表达。结果与正常组比,模型组大鼠造模后48 h肝脏MDA含量较正常组明显升高,SOD、GSH-Px、CAT活性明显降低,肝组织Nrf2mRNA及蛋白、SOD、CATmRNA的表达明显降低(P<0.05);与模型组比,三黄柴术方各剂量组及UDCA组肝脏MDA含量明显下降,SOD、GSH-Px及CAT的活性全部或部分升高,Nrf2mRNA及蛋白、SOD、CATmRNA的表达明显升高(P<0.05)。结论三黄柴术方对急性肝内胆汁淤积淤积性肝损伤的疗效机制可能是通过增加Nrf2蛋白及mRNA的表达,加强肝脏抗氧化应激能力,减轻肝组织炎症损伤。

基于“三焦气化”理论探讨谢春光教授治疗痛风经验

痛风是由单钠尿酸盐沉积所致的晶体相关性关节病,现代医学药物治疗主要以碱化尿液、降尿酸和促尿酸排泄等为主,不良反应较多;而中医药治疗痛风颇具特色,谢春光教授总结30余年临床相关经验,注重三焦气化理论,立足于肺、脾、肾,提出通利三焦以促机体脏腑经络功能恢复,用药灵活,为中医药治疗痛风提供了新的思路。

参苓白术散联合穴位贴敷治疗腹泻型肠易激综合征疗效观察

目的 本研究旨在评估参苓白术散结合穴位贴敷疗法在腹泻型肠易激综合征(irritable bowel syndrome with diarrhea,IBS-D)治疗中的临床效果。方法 选取2022年5月-2023年8月期间就诊于安徽中医药大学第二附属医院脾胃病科门诊与住院部的肠易激综合征腹泻型的患者88例,采用随机数字表法分组为对照组与研究组,各44例。在控制日常饮食与适量运动的前提下,对照组采用盐酸洛哌丁胺治疗,而研究组则应用参苓白术散与穴位贴敷的联合疗法。经过为期28 d的治疗周期,对2组腹泻型肠易激综合征患者的治疗效果进行全面评估。结果 研究组的临床治疗效果在总有效率上明显优于对照组,差异具有统计学意义(P<0.05),其腹胀、排便频率、性状及腹痛程度的改善情况均优于对照组(P<0.05)。经过治疗周期后,2组的血清5-羟色胺(5-HT)以及神经肽Y(NPY)指标均呈现下降趋势,差异具有统计学意义(P<0.05)。在不良反应方面,2组无统计学差异(P>0.05)。对2组中治疗有效的患者进行随访,研究组复发率4.76%(2/42)低于对照组20.00%(7/35)(P<0.05)。结论 参苓白术散联合穴位贴敷治疗腹泻型肠易激综合征具有显著疗效,在改善临床症状和实验室指标方面具有明显优势,且复发率低。

华西广医1号急性毒性、鼻黏膜刺激性和增强免疫功能的研究

为初步了解拟开发医院雾化制剂华西广医1号的安全性和有效性,通过小鼠急性毒性试验、大鼠鼻黏膜刺激性试验和增强免疫功能的药效学试验方法研究了华西广医1号安全性和药效。结果表明:以0.34 g/mL药液24 h内雾化给药,对小鼠无明显急性毒性;对大鼠经鼻腔给药无明显刺激性;以0.068 g/mL雾化给药,有升高幼龄小鼠的胸腺指数的作用趋势;可升高环磷酰胺致免疫功能低下小鼠的巨噬细胞碳粒廓清指数K、校正指数α和外周血白细胞数目(white blood cell,WBC),红细胞数目(red blood cell,RBC)和血红蛋白浓度(hemoglobin,HGB)水平,并可升高溶血素值。可见,华西广医1号雾化给药小鼠的最大给药量为34 g/kg,无明显鼻黏膜刺激性,0.068 g/mL雾化给药有增强免疫功能,可用于预防上呼吸道感染。

青黄散治疗髓系肿瘤的临床研究进展

髓系肿瘤(Myeloid neoplasms)是一组异质性疾病,肿瘤细胞起源于骨髓中具有多向分化潜能的造血干细胞,并出现克隆性增殖。从20世纪70年代中期开始,诸多经典中药方剂,临床运用效果明显,而青黄散作为代表,通过大量临床数据证实该方治疗髓系肿瘤有效,为古方中药的发掘与研究提供指引,然而如何将中医的研究成果科学化、全球化,是机遇也是挑战。

基于面肌超声指标评价针药结合治疗周围性面瘫所致面肌萎缩疗效

目的:基于面肌超声指标评价针药结合治疗周围性面瘫所致面肌萎缩的疗效。方法:选取146例周围性面瘫所致面肌萎缩患者,按照随机数字表法分为对照组和针药结合组各73例。研究过程中2组各剔除3例,最终均纳入70例。对照组采用针刺治疗,针药结合组在对照组基础上加用牵正散加减治疗。10 d为1个疗程,2组均治疗3个疗程。比较2组临床疗效、症状体征评分、面部神经功能评分、面肌超声指标及异常超声发生率,分析症状体征评分、面部神经功能评分与面肌超声指标之间的相关性。结果:治疗后,针药结合组总有效率95.71%,高于对照组84.29%,差异有统计学意义(P<0.05)。2组症状体征、面部神经功能评分均较治疗前降低,针药结合组症状体征、面部神经功能评分均低于对照组,差异均有统计学意义(P<0.05)。2组患侧额肌、降口角肌、降下唇肌厚度均较治疗前增加,针药结合组患侧额肌、降口角肌、降下唇肌厚度均大于对照组,差异均有统计学意义(P<0.05)。2组回声信号增强,肌纤维模糊、增粗发生率均较治疗前降低,针药结合组回声信号增强,肌纤维模糊、增粗发生率均低于对照组,差异均有统计学意义(P<0.05)。症状体征评分、面部神经功能评分与各项面肌超声指标均呈负相关,差异均有统计学意义(P<0.05)。结论:针药结合治疗周围性面瘫所致面肌萎缩疗效显著,能有效改善患者的临床症状和面部神经功能,面肌厚度、异常超声表现等指标对疗效有一定的评估价值。