The oligosaccharide elicitor from the mycelial wall of an endophytic Colletotrichum sp. B501 promoted the production of artemisinin in Artemisia annua L. hairy root culture. When hairy roots of 22-day-old cultures (la...The oligosaccharide elicitor from the mycelial wall of an endophytic Colletotrichum sp. B501 promoted the production of artemisinin in Artemisia annua L. hairy root culture. When hairy roots of 22-day-old cultures (later growth phase) were exposed to the elicitor (20 mg/L) for 4 d, the maximum content of artemisinin reached 1.15 mg/g, a 64.29% increment over the control. The electron X-ray microanalysis disclosed the rapid accumulation of Ca 2+ in the elicited cortical cells of hairy root. The electronic microscope observation revealed the high electron density area in vacuole of elicited cells. During the first day of elicitation the peroxidase activity of hairy roots was improved sharply. Some cellular morphological changes including cell shrinkage, condensation of cytoplasm and nuclear fragmentation, coincident with the appearance of DNA ladders, were observed after the third day of elicitation. It was suggested that the oligosaccharide elicitor triggered the programmed cell death, which may provide the substance or chemical signal for artemisinin biosynthesis.展开更多
Confucianism (especially its teaching theory) is the core of Chinese traditional culture with Confucius as its representative. It has played an indispensable role in the development of Chinese culture. It is the spi...Confucianism (especially its teaching theory) is the core of Chinese traditional culture with Confucius as its representative. It has played an indispensable role in the development of Chinese culture. It is the spiritual pillar of Chinese sustainable development. There are no others that can compare with its teaching method and art. The famous "heuristic teaching theory" is one of its greatest inventions. But many people think that it is Socrates invention. In fact, Socrates "elicitation teaching theory" was invented far later than that of Confucius. This paper aims at investigating of these two theories.展开更多
In decision modeling with influence diagrams, the most challenging task is probability elicitation from domain experts. It is usually very difficult for experts to directly assign precise probabilities to chance nodes...In decision modeling with influence diagrams, the most challenging task is probability elicitation from domain experts. It is usually very difficult for experts to directly assign precise probabilities to chance nodes. In this paper, we propose an approach to elicit probability effectively by using the concept of interval probability (IP). During the elicitation process, a group of experts assign intervals to probabilities instead of assigning exact values. Then the intervals are combined and converted into the point valued probabilities. The detailed steps of the elicitation process are given and illustrated by constructing the influence diagram for employee recruitment decision for a China’s IT Company. The proposed approach provides a convenient and comfortable way for experts to assess probabilities. It is useful in influence diagrams modeling as well as in other subjective probability elicitation situations.展开更多
Nowadays, many scholars would like to use evidence derived from elicitation and introspection in their research. Both elicitation and introspection are common methods of data collection. The essay discusses the useful...Nowadays, many scholars would like to use evidence derived from elicitation and introspection in their research. Both elicitation and introspection are common methods of data collection. The essay discusses the usefulness and pitfall of elicitation and introspection. It finds out that both elicitation and introspection have their own advantages and disadvantages.展开更多
Requirements elicitation is a fundamental phase of software development in which an analyst discovers the needs of different stakeholders and transforms them into requirements.This phase is cost-and time-intensive,and...Requirements elicitation is a fundamental phase of software development in which an analyst discovers the needs of different stakeholders and transforms them into requirements.This phase is cost-and time-intensive,and a project may fail if there are excessive costs and schedule overruns.COVID-19 has affected the software industry by reducing interactions between developers and customers.Such a lack of interaction is a key reason for the failure of software projects.Projects can also fail when customers do not know precisely what they want.Furthermore,selecting the unsuitable elicitation technique can also cause project failure.The present study,therefore,aimed to identify which requirements elicitation technique is the most cost-effective for large-scale projects when time to market is a critical issue or when the customer is not available.To that end,we conducted a systematic literature review on requirements elicitation techniques.Most primary studies identified introspection as the best technique,followed by survey and brainstorming.This finding suggests that introspection should be the first choice of elicitation technique,especially when the customer is not available or the project has strict time and cost constraints.Moreover,introspection should also be used as the starting point in the elicitation process of a large-scale project,and all known requirements should be elicited using this technique.展开更多
In order to develop quality software that meets the originals needs of its users, it is necessary to perform the Requirements Engineering, so that the software context to be developed is identified, examined and speci...In order to develop quality software that meets the originals needs of its users, it is necessary to perform the Requirements Engineering, so that the software context to be developed is identified, examined and specified properly. However, there is a problem that is increasingly in debate: the difficulty in understanding and establishing the purpose of the software to be developed, as pointed out by important researches in the area, such as the Chaos Report, which indicates that only 29% of software projects are successful, and the Software Engineering Institute, which points out software requirements as a critical factor for the success of software engineering and that deficiencies in this dimension are the main causes of software project failures. This article presents a proposal to address this problem through the use of the Hermeneutical Elicitation of Requirements, which is the conceptual adequacy of some hermeneutical methods in a technical approach that assists the requirements engineer to conceive better of the software requirements. In this way, the software engineer will be better able to develop the software to better meet the needs of its end users and sponsors.展开更多
Rosemary (<em>Rosmrinus officinals</em>) is one of the most important medicinal plants was cultivated for two subsequent seasons field experiment trial (March 2018, 2019) designed as factorial split-plot d...Rosemary (<em>Rosmrinus officinals</em>) is one of the most important medicinal plants was cultivated for two subsequent seasons field experiment trial (March 2018, 2019) designed as factorial split-plot design with three replicates. The main factor 4 biotic elicitors: control (E<sub>1</sub>), phenylalanine (E<sub>2</sub>), yeast (E<sub>3</sub>) and (E4), E2 + F3 Whereas, the sub-main factor, four fertilizers: (F1) NPK, (F2) 1/2NPK + PGPB and humic acid + PGPB (F<sub>3</sub>), and moringa dry leaves extract. + PGPB (F<sub>4</sub>). Statistical analysis for collected data revealed significant promotion for growth traits leading to significant increment biomass yield, secondary metabolites production and quality. Total phenolics, total flavonoids and essential oil its terpenes contents in which solitary, E2, E3 acted positive significant impact while E4exhiboted significant positive impact over E1 whereas, F1-4 achieved significant increment in which, biofertilser f4 > f3 > f2 over NPK biofertilser, while paired E1-4 with F1-4 performed E4E4 exceeded E3F3 exceeded E3F2 that exceeded E1F1. Therefore, multi-repeating elicitation with E2, 3, 4 coupled with Biofertilizers F2, 4 could be considered as eco-friendly innovative reliable practical application for sustainable improvement and sustainable use that exceeded significantly over traditional agriculture NPK alone or 1/2 NPK-PGPB for <em>R. officinals</em>.展开更多
Zhejiang Province firmly seized the opportunity of worldwide economy integration and manufacture industry strategically transfer to renew the ideas, execute the measures and forge ahead innovation so that it practical...Zhejiang Province firmly seized the opportunity of worldwide economy integration and manufacture industry strategically transfer to renew the ideas, execute the measures and forge ahead innovation so that it practically .developed the superiority of private enterprise system, government service, industry zone, enterprise competition, commodity brand, and culture and ideas and soon formed six manufacture development modes: grass root economy mode, block economy mode, demi-wolf economy mode, scaled economy mode, marketing economy mode and extroverted economy mode, which help Zhejiang province be a big manufacture province and economic prosperous province in China.展开更多
A challenge for many School-Based Family Counseling(SBFC)practitioners,child psychotherapists and researchers are finding ways to give voice to children and eliciting trustworthy and detailed narratives that could ser...A challenge for many School-Based Family Counseling(SBFC)practitioners,child psychotherapists and researchers are finding ways to give voice to children and eliciting trustworthy and detailed narratives that could serve as resource for understanding the needs of young clients in the context of all their interpersonal networks.Children are often reticent when asked to self-disclose and tell their stories during consultation.The purpose of the present study was to examine the utility of five sequential steps constituting the Collage Life Story Elicitation Technique(CLET)for scaffolding storytelling among children in middle childhood(aged 9-12 years).Using the CLET for data collection and conducting an interpretive analysis,the researchers explored the performance of 38 middle-childhood children living in three different settings.Findings suggest that the five sequential steps of the CLET adequately and satisfactorily combine to stimulate and elicit rich data and help children to construct their narratives and represent the challenges they face in everyday living.We discuss the application of CLET in SBFC practice as tool when screening and intervention planning for children’s perspectives pertaining to a range of topics regarding each of the four quadrants as proposed in the SBFC metamodel.展开更多
需求获取和建模是需求工程中的关键步骤,影响后续系统设计与实现.传统的需求获取和建模方法通常由需求提供者、需求分析师等多类干系人共同协作、反复迭代完成,需要耗费大量的人力.如何减轻需求提供者与需求分析师的负担、提高获取和建...需求获取和建模是需求工程中的关键步骤,影响后续系统设计与实现.传统的需求获取和建模方法通常由需求提供者、需求分析师等多类干系人共同协作、反复迭代完成,需要耗费大量的人力.如何减轻需求提供者与需求分析师的负担、提高获取和建模的效率有着重要意义.现有工作中有的使用知识库来提供更多知识,以辅助获取或者建模,有的利用自然语言处理等技术对获取或者建模过程进行自动化,但是它们并没有减轻需求提供者的负担.利用大语言模型(large language models,LLMs)的生成能力,提供了一种人机协作的迭代式需求获取和建模框架ChatModeler.具体来说,根据真实世界中需求团队的分工及协作关系,将部分需求提供者、需求分析师等角色的工作由大语言模型承担,而需求提供者只需要进行确认.为大语言模型扮演的各种角色进行了提示词设计,该提示词会随需求的元模型而变化.ChatModeler在7个需求案例上与3种需求模型的自动建模方法进行了14组对比实验,证明了ChatModeler在降低需求提供者的负担和生成高质量需求模型2个方面上的优越性.展开更多
目的探索以执业能力为导向的导师负责制在骨科实习生培养中的应用效果。方法选取2021年9月—2023年6月2021和2022学年“5+3”一体化的60名本科实习生作为研究对象,将2021学年的实习生作为对照组,2022学年的实习生作为研究组,每组30名。...目的探索以执业能力为导向的导师负责制在骨科实习生培养中的应用效果。方法选取2021年9月—2023年6月2021和2022学年“5+3”一体化的60名本科实习生作为研究对象,将2021学年的实习生作为对照组,2022学年的实习生作为研究组,每组30名。对照组采用标准教学模式培养,研究组采用以执业能力为导向的导师负责制模式培养,培养时间为1个月。比较2组实习生的理论和临床考核成绩、迷你临床演练评估量表(mini-clinical evaluation exercise,Mini-CEX)评分、主观-客观-评价-计划(subject-objective-assessmentplan,SOAP)评分、医患沟通技能评价量表(set elicit give understand end framework,SEGUE)评分。结果2组实习生的客观题和临床技能考核成绩比较,差异无统计学意义(P>0.05);研究组实习生的病例分析题成绩、Mini-CEX评分、SOAP评分和SEGUE评分([41.3±3.5)分、(47.9±7.0)分、(74.4±7.7)分、(20.5±3.2)分]高于对照组[(36.8±6.1)分、(36.0±7.0)分、(62.0±7.1)分、(18.1±3.0)分],差异均有统计学意义(P<0.05)。结论以执业能力为导向的导师负责制能够明显提高骨科实习生的临床诊疗水平,有助于培养出更优秀的医学人才。展开更多
文摘The oligosaccharide elicitor from the mycelial wall of an endophytic Colletotrichum sp. B501 promoted the production of artemisinin in Artemisia annua L. hairy root culture. When hairy roots of 22-day-old cultures (later growth phase) were exposed to the elicitor (20 mg/L) for 4 d, the maximum content of artemisinin reached 1.15 mg/g, a 64.29% increment over the control. The electron X-ray microanalysis disclosed the rapid accumulation of Ca 2+ in the elicited cortical cells of hairy root. The electronic microscope observation revealed the high electron density area in vacuole of elicited cells. During the first day of elicitation the peroxidase activity of hairy roots was improved sharply. Some cellular morphological changes including cell shrinkage, condensation of cytoplasm and nuclear fragmentation, coincident with the appearance of DNA ladders, were observed after the third day of elicitation. It was suggested that the oligosaccharide elicitor triggered the programmed cell death, which may provide the substance or chemical signal for artemisinin biosynthesis.
文摘Confucianism (especially its teaching theory) is the core of Chinese traditional culture with Confucius as its representative. It has played an indispensable role in the development of Chinese culture. It is the spiritual pillar of Chinese sustainable development. There are no others that can compare with its teaching method and art. The famous "heuristic teaching theory" is one of its greatest inventions. But many people think that it is Socrates invention. In fact, Socrates "elicitation teaching theory" was invented far later than that of Confucius. This paper aims at investigating of these two theories.
文摘In decision modeling with influence diagrams, the most challenging task is probability elicitation from domain experts. It is usually very difficult for experts to directly assign precise probabilities to chance nodes. In this paper, we propose an approach to elicit probability effectively by using the concept of interval probability (IP). During the elicitation process, a group of experts assign intervals to probabilities instead of assigning exact values. Then the intervals are combined and converted into the point valued probabilities. The detailed steps of the elicitation process are given and illustrated by constructing the influence diagram for employee recruitment decision for a China’s IT Company. The proposed approach provides a convenient and comfortable way for experts to assess probabilities. It is useful in influence diagrams modeling as well as in other subjective probability elicitation situations.
文摘Nowadays, many scholars would like to use evidence derived from elicitation and introspection in their research. Both elicitation and introspection are common methods of data collection. The essay discusses the usefulness and pitfall of elicitation and introspection. It finds out that both elicitation and introspection have their own advantages and disadvantages.
基金funding this work through research group no.RG-1441-490.
文摘Requirements elicitation is a fundamental phase of software development in which an analyst discovers the needs of different stakeholders and transforms them into requirements.This phase is cost-and time-intensive,and a project may fail if there are excessive costs and schedule overruns.COVID-19 has affected the software industry by reducing interactions between developers and customers.Such a lack of interaction is a key reason for the failure of software projects.Projects can also fail when customers do not know precisely what they want.Furthermore,selecting the unsuitable elicitation technique can also cause project failure.The present study,therefore,aimed to identify which requirements elicitation technique is the most cost-effective for large-scale projects when time to market is a critical issue or when the customer is not available.To that end,we conducted a systematic literature review on requirements elicitation techniques.Most primary studies identified introspection as the best technique,followed by survey and brainstorming.This finding suggests that introspection should be the first choice of elicitation technique,especially when the customer is not available or the project has strict time and cost constraints.Moreover,introspection should also be used as the starting point in the elicitation process of a large-scale project,and all known requirements should be elicited using this technique.
文摘In order to develop quality software that meets the originals needs of its users, it is necessary to perform the Requirements Engineering, so that the software context to be developed is identified, examined and specified properly. However, there is a problem that is increasingly in debate: the difficulty in understanding and establishing the purpose of the software to be developed, as pointed out by important researches in the area, such as the Chaos Report, which indicates that only 29% of software projects are successful, and the Software Engineering Institute, which points out software requirements as a critical factor for the success of software engineering and that deficiencies in this dimension are the main causes of software project failures. This article presents a proposal to address this problem through the use of the Hermeneutical Elicitation of Requirements, which is the conceptual adequacy of some hermeneutical methods in a technical approach that assists the requirements engineer to conceive better of the software requirements. In this way, the software engineer will be better able to develop the software to better meet the needs of its end users and sponsors.
文摘Rosemary (<em>Rosmrinus officinals</em>) is one of the most important medicinal plants was cultivated for two subsequent seasons field experiment trial (March 2018, 2019) designed as factorial split-plot design with three replicates. The main factor 4 biotic elicitors: control (E<sub>1</sub>), phenylalanine (E<sub>2</sub>), yeast (E<sub>3</sub>) and (E4), E2 + F3 Whereas, the sub-main factor, four fertilizers: (F1) NPK, (F2) 1/2NPK + PGPB and humic acid + PGPB (F<sub>3</sub>), and moringa dry leaves extract. + PGPB (F<sub>4</sub>). Statistical analysis for collected data revealed significant promotion for growth traits leading to significant increment biomass yield, secondary metabolites production and quality. Total phenolics, total flavonoids and essential oil its terpenes contents in which solitary, E2, E3 acted positive significant impact while E4exhiboted significant positive impact over E1 whereas, F1-4 achieved significant increment in which, biofertilser f4 > f3 > f2 over NPK biofertilser, while paired E1-4 with F1-4 performed E4E4 exceeded E3F3 exceeded E3F2 that exceeded E1F1. Therefore, multi-repeating elicitation with E2, 3, 4 coupled with Biofertilizers F2, 4 could be considered as eco-friendly innovative reliable practical application for sustainable improvement and sustainable use that exceeded significantly over traditional agriculture NPK alone or 1/2 NPK-PGPB for <em>R. officinals</em>.
文摘Zhejiang Province firmly seized the opportunity of worldwide economy integration and manufacture industry strategically transfer to renew the ideas, execute the measures and forge ahead innovation so that it practically .developed the superiority of private enterprise system, government service, industry zone, enterprise competition, commodity brand, and culture and ideas and soon formed six manufacture development modes: grass root economy mode, block economy mode, demi-wolf economy mode, scaled economy mode, marketing economy mode and extroverted economy mode, which help Zhejiang province be a big manufacture province and economic prosperous province in China.
文摘A challenge for many School-Based Family Counseling(SBFC)practitioners,child psychotherapists and researchers are finding ways to give voice to children and eliciting trustworthy and detailed narratives that could serve as resource for understanding the needs of young clients in the context of all their interpersonal networks.Children are often reticent when asked to self-disclose and tell their stories during consultation.The purpose of the present study was to examine the utility of five sequential steps constituting the Collage Life Story Elicitation Technique(CLET)for scaffolding storytelling among children in middle childhood(aged 9-12 years).Using the CLET for data collection and conducting an interpretive analysis,the researchers explored the performance of 38 middle-childhood children living in three different settings.Findings suggest that the five sequential steps of the CLET adequately and satisfactorily combine to stimulate and elicit rich data and help children to construct their narratives and represent the challenges they face in everyday living.We discuss the application of CLET in SBFC practice as tool when screening and intervention planning for children’s perspectives pertaining to a range of topics regarding each of the four quadrants as proposed in the SBFC metamodel.
文摘需求获取和建模是需求工程中的关键步骤,影响后续系统设计与实现.传统的需求获取和建模方法通常由需求提供者、需求分析师等多类干系人共同协作、反复迭代完成,需要耗费大量的人力.如何减轻需求提供者与需求分析师的负担、提高获取和建模的效率有着重要意义.现有工作中有的使用知识库来提供更多知识,以辅助获取或者建模,有的利用自然语言处理等技术对获取或者建模过程进行自动化,但是它们并没有减轻需求提供者的负担.利用大语言模型(large language models,LLMs)的生成能力,提供了一种人机协作的迭代式需求获取和建模框架ChatModeler.具体来说,根据真实世界中需求团队的分工及协作关系,将部分需求提供者、需求分析师等角色的工作由大语言模型承担,而需求提供者只需要进行确认.为大语言模型扮演的各种角色进行了提示词设计,该提示词会随需求的元模型而变化.ChatModeler在7个需求案例上与3种需求模型的自动建模方法进行了14组对比实验,证明了ChatModeler在降低需求提供者的负担和生成高质量需求模型2个方面上的优越性.
文摘目的探索以执业能力为导向的导师负责制在骨科实习生培养中的应用效果。方法选取2021年9月—2023年6月2021和2022学年“5+3”一体化的60名本科实习生作为研究对象,将2021学年的实习生作为对照组,2022学年的实习生作为研究组,每组30名。对照组采用标准教学模式培养,研究组采用以执业能力为导向的导师负责制模式培养,培养时间为1个月。比较2组实习生的理论和临床考核成绩、迷你临床演练评估量表(mini-clinical evaluation exercise,Mini-CEX)评分、主观-客观-评价-计划(subject-objective-assessmentplan,SOAP)评分、医患沟通技能评价量表(set elicit give understand end framework,SEGUE)评分。结果2组实习生的客观题和临床技能考核成绩比较,差异无统计学意义(P>0.05);研究组实习生的病例分析题成绩、Mini-CEX评分、SOAP评分和SEGUE评分([41.3±3.5)分、(47.9±7.0)分、(74.4±7.7)分、(20.5±3.2)分]高于对照组[(36.8±6.1)分、(36.0±7.0)分、(62.0±7.1)分、(18.1±3.0)分],差异均有统计学意义(P<0.05)。结论以执业能力为导向的导师负责制能够明显提高骨科实习生的临床诊疗水平,有助于培养出更优秀的医学人才。