For Alzheimer's disease(AD),the earliest symptom is memory loss,which runs throughout the disease.The mutual growth and decline of long-term potentiation(LTP)and long-term depression(LTD)in synaptic plasticity is ...For Alzheimer's disease(AD),the earliest symptom is memory loss,which runs throughout the disease.The mutual growth and decline of long-term potentiation(LTP)and long-term depression(LTD)in synaptic plasticity is the basic condition of learning and memory.In the early stage of AD,there is no significant change in synaptic plasticity,so the treatment should mainly focus on warming yang and replenishing qi.In the middle stage of AD,LTP is continuously damaged and reduced,and synaptic plasticity is destroyed,such as weakness of the spleen and stomach,causing stagnation of endogenous retention of phlegm in the brain,so the treatment should focus on enriching yin and tonifying yang,resolving phlegm to remove stagnation.In the late stage of AD,due to excessive deposition of amyloid and other metabolites,neurotoxins are produced and brain atrophy is aggravated,such as depression leading to stasis,phlegm and stasis binding together,long term of stasis turning to heat toxin,which invades the brain,excessive heat damages yin,then both yin and yang are damaged,finally,the brain collateral loses nutrition,so the treatment should focus on replenishing essence and reinforcing marrow,detoxifying and resolving stasis.Yisui Fuyong Decoction can replenish essence and reinforce marrow to nourish yin,warm yang and tonify qi to strengthen yang,combine phlegm resolving,stasis elimination and collateral vessels dredging,realizing the treatment effects in all three stages of synaptic plasticity.展开更多
目的探究通经补肾益髓汤联合调督理筋针法对腰椎间盘突出症患者的疗效及对血清前列腺素E2(Prostaglandin E2,PGE2)、血清磷酸化p38丝裂原活化蛋白激酶(p38 mitogen activated protein kinase,p-p38MAPK)水平的影响。方法选取医院收治的...目的探究通经补肾益髓汤联合调督理筋针法对腰椎间盘突出症患者的疗效及对血清前列腺素E2(Prostaglandin E2,PGE2)、血清磷酸化p38丝裂原活化蛋白激酶(p38 mitogen activated protein kinase,p-p38MAPK)水平的影响。方法选取医院收治的腰椎间盘突出症患者150例,将其随机分为对照组和观察组,两组各75例。对照组给予常规西药治疗,观察组给予通经补肾益髓汤联合调督理筋针法治疗,两组疗程均为4周,对比治疗后的临床治疗效果、视觉模拟评分法(visual analogue scale,VAS)、Oswestry功能障碍指数(Oswestry disability index,ODI)、腰椎间隙和腰椎活动度、血清PGE2、p-P38MAPK水平、健康调查简表(the MOS item short from health survey,SF-36)评分。结果两组患者经4周治疗,观察组总有效率为96.00%(72/75),较对照组(84.00%,63/75)有显著提高(P<0.05);治疗后,VAS评分、ODI指数均较治疗前显著降低(P<0.05),且观察组降低程度显著优于对照组(P<0.05);治疗后两组腰椎间隙高度较治疗前升高(P<0.05),腰椎活动度均较治疗前降低,且观察组显著优于对照组(P<0.05);治疗后,血清PGE2水平较治疗前显著降低,血清p-P38MAPK水平较治疗前显著升高(P<0.05),同时观察组较对照组改善显著(P<0.05);两组患者治疗后SF-36量表各条目评分较治疗前均显著提高(P<0.05),观察组提高程度更明显(P<0.05)。结论通经补肾益髓汤联合调督理筋针法能显著提高腰椎间盘突出症患者的临床治疗效果,改善患者疼痛及活动功能,显著降低血清PGE2水平,提高p-P38MAPK水平,提高患者生活质量。展开更多
Shouwu is a traditional Chinese medicine(TCM)with neuroprotective effect.Shouwu Yizhi decoction(SYD)was designed based on TCM theory.However,little is known about the roles of SYD in Vascular dementia(Va D).The presen...Shouwu is a traditional Chinese medicine(TCM)with neuroprotective effect.Shouwu Yizhi decoction(SYD)was designed based on TCM theory.However,little is known about the roles of SYD in Vascular dementia(Va D).The present study aimed to evaluate the potential effects of SYD on the vascular cognitive impairment and explore the underlying mechanism by establishing focal cerebral ischemia/reperfusion(I/R)rat model to induce Va D.SYD administration(54 mg·kg^(-1))for 40 days obviously improved the vascular cognitive impairment in the middle cerebral artery occlusion(MCAO)rats as evidenced by the declined neurological deficit score and shortened escape latency via neurological deficit assessment and Morris water maze test.Moreover,SYD decreased neuron damage-induced cell death and ameliorated the ultrastructure of endothelial cells in the MCAO rats,thereby alleviating Va D.Mechanistically,SYD caused increases in the expression of vascular endothelial growth factor(VEGF),CD34 and CD31,compared with the MCAO rats in coronal hippocampus.Simultaneously,the expression level of mi R-210 was elevated significantly after SYD administration,compared with the vehicle rats(P<0.01).The expression of Notch 4 at both m RNA and protein levels was upregulated remarkably along with the notably downregulated DLL4 expression under SYD administration compared with the vehicle rats(P<0.05).Overall,the above results indicated that SYD promoted angiogenesis by upregulating VEGF-induced mi R210 expression to activate Notch pathway,and further alleviated neuron damage and ameliorated the ultrastructure of endothelial cells in the MCAO rats,ultimately enhancing the cognition and memory of MCAO rats.Therefore,our findings preliminarily identified the effect and the mechanism of action for SYD on Va D in rats.SYD could be a potential candidate in treatment of Va D.展开更多
基金General Program of Chongqing Municipal Natural Science Foundation(cstc2020jcyj-msxmX0011)Chongqing Medical Scientific Research Project(Joint Project of Chongqing Health Commission and Science and Technology Bureau,2020ZY023596)the First Batch of Young Outstanding Talent Project of Chongqing Traditional Chinese Medicine Hospital(Yu Zhong Yi Yuan Dang[2020]No.20).
文摘For Alzheimer's disease(AD),the earliest symptom is memory loss,which runs throughout the disease.The mutual growth and decline of long-term potentiation(LTP)and long-term depression(LTD)in synaptic plasticity is the basic condition of learning and memory.In the early stage of AD,there is no significant change in synaptic plasticity,so the treatment should mainly focus on warming yang and replenishing qi.In the middle stage of AD,LTP is continuously damaged and reduced,and synaptic plasticity is destroyed,such as weakness of the spleen and stomach,causing stagnation of endogenous retention of phlegm in the brain,so the treatment should focus on enriching yin and tonifying yang,resolving phlegm to remove stagnation.In the late stage of AD,due to excessive deposition of amyloid and other metabolites,neurotoxins are produced and brain atrophy is aggravated,such as depression leading to stasis,phlegm and stasis binding together,long term of stasis turning to heat toxin,which invades the brain,excessive heat damages yin,then both yin and yang are damaged,finally,the brain collateral loses nutrition,so the treatment should focus on replenishing essence and reinforcing marrow,detoxifying and resolving stasis.Yisui Fuyong Decoction can replenish essence and reinforce marrow to nourish yin,warm yang and tonify qi to strengthen yang,combine phlegm resolving,stasis elimination and collateral vessels dredging,realizing the treatment effects in all three stages of synaptic plasticity.
文摘目的探究通经补肾益髓汤联合调督理筋针法对腰椎间盘突出症患者的疗效及对血清前列腺素E2(Prostaglandin E2,PGE2)、血清磷酸化p38丝裂原活化蛋白激酶(p38 mitogen activated protein kinase,p-p38MAPK)水平的影响。方法选取医院收治的腰椎间盘突出症患者150例,将其随机分为对照组和观察组,两组各75例。对照组给予常规西药治疗,观察组给予通经补肾益髓汤联合调督理筋针法治疗,两组疗程均为4周,对比治疗后的临床治疗效果、视觉模拟评分法(visual analogue scale,VAS)、Oswestry功能障碍指数(Oswestry disability index,ODI)、腰椎间隙和腰椎活动度、血清PGE2、p-P38MAPK水平、健康调查简表(the MOS item short from health survey,SF-36)评分。结果两组患者经4周治疗,观察组总有效率为96.00%(72/75),较对照组(84.00%,63/75)有显著提高(P<0.05);治疗后,VAS评分、ODI指数均较治疗前显著降低(P<0.05),且观察组降低程度显著优于对照组(P<0.05);治疗后两组腰椎间隙高度较治疗前升高(P<0.05),腰椎活动度均较治疗前降低,且观察组显著优于对照组(P<0.05);治疗后,血清PGE2水平较治疗前显著降低,血清p-P38MAPK水平较治疗前显著升高(P<0.05),同时观察组较对照组改善显著(P<0.05);两组患者治疗后SF-36量表各条目评分较治疗前均显著提高(P<0.05),观察组提高程度更明显(P<0.05)。结论通经补肾益髓汤联合调督理筋针法能显著提高腰椎间盘突出症患者的临床治疗效果,改善患者疼痛及活动功能,显著降低血清PGE2水平,提高p-P38MAPK水平,提高患者生活质量。
基金supported by the National Natural Science Foundation of China(Nos.81302940,81373670 and 30873323)the Natural Science Foundation of Shandong Province(ZR2015PH046,Y2005C43)+3 种基金Science and Technology Development Grant of the State Administration of TCM of Shandong(2011-192,2015-286,2013-216)Family Planning Committee of Shandong Province([2014]14)the Primary Research&Development Plan of Shandong Province(2016GSF202016)the Innovation Project of Shandong Academy of Medical Sciences
文摘Shouwu is a traditional Chinese medicine(TCM)with neuroprotective effect.Shouwu Yizhi decoction(SYD)was designed based on TCM theory.However,little is known about the roles of SYD in Vascular dementia(Va D).The present study aimed to evaluate the potential effects of SYD on the vascular cognitive impairment and explore the underlying mechanism by establishing focal cerebral ischemia/reperfusion(I/R)rat model to induce Va D.SYD administration(54 mg·kg^(-1))for 40 days obviously improved the vascular cognitive impairment in the middle cerebral artery occlusion(MCAO)rats as evidenced by the declined neurological deficit score and shortened escape latency via neurological deficit assessment and Morris water maze test.Moreover,SYD decreased neuron damage-induced cell death and ameliorated the ultrastructure of endothelial cells in the MCAO rats,thereby alleviating Va D.Mechanistically,SYD caused increases in the expression of vascular endothelial growth factor(VEGF),CD34 and CD31,compared with the MCAO rats in coronal hippocampus.Simultaneously,the expression level of mi R-210 was elevated significantly after SYD administration,compared with the vehicle rats(P<0.01).The expression of Notch 4 at both m RNA and protein levels was upregulated remarkably along with the notably downregulated DLL4 expression under SYD administration compared with the vehicle rats(P<0.05).Overall,the above results indicated that SYD promoted angiogenesis by upregulating VEGF-induced mi R210 expression to activate Notch pathway,and further alleviated neuron damage and ameliorated the ultrastructure of endothelial cells in the MCAO rats,ultimately enhancing the cognition and memory of MCAO rats.Therefore,our findings preliminarily identified the effect and the mechanism of action for SYD on Va D in rats.SYD could be a potential candidate in treatment of Va D.
