Background:Hyperlipidemia(HLP)is a disorder in which lipids in the blood are disturbed due to abnormal lipid metabolism.Statins and fibrates lipid-regulating drugs prevent the generation of lipids and improves HLP,the...Background:Hyperlipidemia(HLP)is a disorder in which lipids in the blood are disturbed due to abnormal lipid metabolism.Statins and fibrates lipid-regulating drugs prevent the generation of lipids and improves HLP,thereby reducing the likelihood of developing cardiovascular and cerebrovascular diseases.However,their adverse reactions and side effects limit a wide range of clinical applications.Tibetan medicine compound preparation Siwei Yuganzi decoction(SYD)is an adaptation of a classical prescription of the Tibetan medicine Sanguo decoction,that has been proved to have the effect of improving lipid metabolism;however,its possible mechanism remains unknown.Methods:Sixty specific-pathogen free grade Sprague Dawley rats were randomly divided into the blank control group,the HLP group,the Chinese patent medicine Xuezhikang positive control group,and the treatment groups with Tibetan SYD high-,middle-,and low-dosages respectively.Ten rats were assigned to each group.Every rat in these groups was fed with a high-fat emulsion to establish the hyperlipidemic animal model,except for those in the blank control group.Then the rats in the blank control and HLP groups were fed with 0.9%normal saline,those in Xuezhikang group were fed with Xuezhikang suspension at the dose of 0.11 g/kg/d,and those in SYD groups were fed with the SYD suspension at the dosage of 16.2 g/kg/d(high dosage),10.8 g/kg/d(middle dosage),and 5.4 g/kg/d(low dosage),respectively.After 8 weeks,the levels of serum total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)were detected by the ELISA.The mRNA and protein expression levels of liver X receptorα(LXRα)and cholesterol 7α-hydroxylase 1(CYP7A1)were detected using RT-PCR,western blotting,and immunohistochemistry.Results:The serum levels of TC,TG,and LDL-C in the HLP group were higher than those in the blank control group(P<0.05),whereas the serum levels of HDL-C and the expression levels of both LXRαand CYP7A1 mRNA and proteins in the liver in the HLP group were lower than those in the control group(P<0.05).After the treatment of the Xuezhikang and SYD,the serum levels of TG,TC,and LDL-C significantly decreased(P<0.05),whereas the serum levels of HDL-C and the expression levels of both LXRαand CYP7A1 mRNA and proteins in the liver significantly increased compared with those in the HLP group(P<0.05).Furthermore,The serum levels of TC,TG,and LDL-C in the SYD group at high dosage were lower(P<0.05),the serum level of HDL-C was higher,and the expression levels of both LXRαand CYP7A1 mRNA and proteins in the liver tissue were higher than those in the Xuezhikang group(P<0.05).Conclusion:SYD can improve the serum levels of TC,TG,and LDL-C in the hyperlipidemic animal model via increasing the expression of both LXRαand CYP7A1 mRNA and proteins in the liver tissue.展开更多
文摘Background:Hyperlipidemia(HLP)is a disorder in which lipids in the blood are disturbed due to abnormal lipid metabolism.Statins and fibrates lipid-regulating drugs prevent the generation of lipids and improves HLP,thereby reducing the likelihood of developing cardiovascular and cerebrovascular diseases.However,their adverse reactions and side effects limit a wide range of clinical applications.Tibetan medicine compound preparation Siwei Yuganzi decoction(SYD)is an adaptation of a classical prescription of the Tibetan medicine Sanguo decoction,that has been proved to have the effect of improving lipid metabolism;however,its possible mechanism remains unknown.Methods:Sixty specific-pathogen free grade Sprague Dawley rats were randomly divided into the blank control group,the HLP group,the Chinese patent medicine Xuezhikang positive control group,and the treatment groups with Tibetan SYD high-,middle-,and low-dosages respectively.Ten rats were assigned to each group.Every rat in these groups was fed with a high-fat emulsion to establish the hyperlipidemic animal model,except for those in the blank control group.Then the rats in the blank control and HLP groups were fed with 0.9%normal saline,those in Xuezhikang group were fed with Xuezhikang suspension at the dose of 0.11 g/kg/d,and those in SYD groups were fed with the SYD suspension at the dosage of 16.2 g/kg/d(high dosage),10.8 g/kg/d(middle dosage),and 5.4 g/kg/d(low dosage),respectively.After 8 weeks,the levels of serum total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)were detected by the ELISA.The mRNA and protein expression levels of liver X receptorα(LXRα)and cholesterol 7α-hydroxylase 1(CYP7A1)were detected using RT-PCR,western blotting,and immunohistochemistry.Results:The serum levels of TC,TG,and LDL-C in the HLP group were higher than those in the blank control group(P<0.05),whereas the serum levels of HDL-C and the expression levels of both LXRαand CYP7A1 mRNA and proteins in the liver in the HLP group were lower than those in the control group(P<0.05).After the treatment of the Xuezhikang and SYD,the serum levels of TG,TC,and LDL-C significantly decreased(P<0.05),whereas the serum levels of HDL-C and the expression levels of both LXRαand CYP7A1 mRNA and proteins in the liver significantly increased compared with those in the HLP group(P<0.05).Furthermore,The serum levels of TC,TG,and LDL-C in the SYD group at high dosage were lower(P<0.05),the serum level of HDL-C was higher,and the expression levels of both LXRαand CYP7A1 mRNA and proteins in the liver tissue were higher than those in the Xuezhikang group(P<0.05).Conclusion:SYD can improve the serum levels of TC,TG,and LDL-C in the hyperlipidemic animal model via increasing the expression of both LXRαand CYP7A1 mRNA and proteins in the liver tissue.
