Cultural Heritage System and Coordinated Protection of Ancient Sichuan Salt Road

Ancient Sichuan Salt Road(the ancient road for the transportation of Sichuan salt) is a cultural route characterized by salt transportation culture in southwest and central-west China, along which a large number of diversifi ed and systematic cultural heritages have been left, including both tangible and intangible salt transportation cultural heritages such as ancient salt transportation road, salt shop, post house, stele inscription, ancient town, ancient bridge, guild hal, and boatmen's work songs. With the rapid economic and social development, the accelerating modernization and urbanization, and also drastic changes of traffi c environment have contributed to the endangered situation of ancient Sichuan salt road cultural heritage. In face of such problems as lack of coordinated protection mechanism, destructive development, backward protection of cultural relics, and inheritance risks of intangible heritage, cultural heritages along the ancient Sichuan salt road should be protected by establishing a protection mechanism, organizing the coordinated actions of local areas along the road, investigating and rescuing cultural heritage in time, and enhancing the cooperative research. In view of the complicate and long-term nature of protecting linear cultural heritage, successful experience of domestic and foreign cultural route heritage management can be learned, salt transportation cultural heritage protection, tourism planning and development of ancient Sichuan salt road should be considered, protection of the ancient salt road should be combined with economic, social and cultural development of regions along the road, so as to explore an effective mode for the protection, development and management of large-scale linear cultural heritages consisting of both land and water routes.

Stability investigation of road cut slope in basaltic rockmass,Mahabaleshwar,India

Slope failures along hill cut road slopes are the major nuisance for commuters and highway planners as they put the human lives at huge risk,coupled with immense monetary losses.Analysis of these vulnerable cut slopes entails the assessment and estimation of the suitable material strength input parameters to be used in the numerical models to accomplish a holistic stability examination.For the present study a 60 m high,basaltic and lateritic road cut hill slope in Mahabaleshwar,India,has been considered.A number of samples of both basalt and laterite,in their natural state were tested in the laboratory and the evaluated maximum,minimum and mean strength parameters were employed for the three cases in a distinct element numerical model.The Mohr-Coulomb failure criterion has been incorporated in the numerical model for the material as well as the joints.The numerical investigation offered the factor of safety and insights into the probable deformational mechanism for the three cases.Beside,several critical parameters have also been judged from the study viz.,mode of failure,factor of safety,shear strain rate,displacement magnitudes etc.The result of this analysis shows that the studied section is prone to recurrent failures due to the capping of a substantially thick layer of weaker lateritic material above the high strength basaltic rock mass.External triggering mechanisms like heavy precipitation and earthquake may also accelerate the slope failure in this area.The study also suggests employing instant preventive measures to avert the further risk of damage.

Dynamic simulation analysis of molten salt reactor-coupled air-steam combined cycle power generation system

A nonlinear dynamic simulation model based on coordinated control of speed and flow rate for the molten salt reactor and combined cycle systems is proposed here to ensure the coordination and stability between the molten salt reactor and power system.This model considers the impact of thermal properties of fluid variation on accuracy and has been validated with Simulink.This study reveals the capability of the control system to compensate for anomalous situations and maintain shaft stability in the event of perturbations occurring in high-temperature molten salt tank outlet parameters.Meanwhile,the control system’s impact on the system’s dynamic characteristics under molten salt disturbance is also analyzed.The results reveal that after the disturbance occurs,the controlled system benefits from the action of the control,and the overshoot and disturbance amplitude are positively correlated,while the system power and frequency eventually return to the initial values.This simulation model provides a basis for utilizing molten salt reactors for power generation and maintaining grid stability.

Failure transition of shear-to-dilation band of rock salt under triaxial stresses

Great potential of underground gas/energy storage in salt caverns seems to be a promising solution to support renewable energy.In the underground storage method,the operating cycle unfortunately may reach up to daily or even hourly,which generates complicated pressures on the salt cavern.Furthermore,the mechanical behavior of rock salt may change and present distinct failure characteristics under different stress states,which affects the performance of salt cavern during the time period of full service.To reproduce a similar loading condition on the cavern surrounding rock mass,the cyclic triaxial loading/unloading tests are performed on the rock salt to explore the mechanical transition behavior and failure characteristics under different confinement.Experimental results show that the rock salt samples pre-sent a diffused shear failure band with significant bulges at certain locations in low confining pressure conditions(e.g.5 MPa,10 MPa and 15 MPa),which is closely related to crystal misorientation and grain boundary sliding.Under the elevated confinement(e.g.20 MPa,30 MPa and 40 MPa),the dilation band dominates the failure mechanism,where the large-size halite crystals are crushed to be smaller size and new pores are developing.The failure transition mechanism revealed in the paper provides additional insight into the mechanical performance of salt caverns influenced by complicated stress states.

Ionization Engineering of Hydrogels Enables Highly Efficient Salt‑Impeded Solar Evaporation and Night‑Time Electricity Harvesting

Interfacial solar evaporation holds immense potential for brine desalination with low carbon footprints and high energy utilization.Hydrogels,as a tunable material platform from the molecular level to the macroscopic scale,have been considered the most promising candidate for solar evaporation.However,the simultaneous achievement of high evaporation efficiency and satisfactory tolerance to salt ions in brine remains a challenging scientific bottleneck,restricting the widespread application.Herein,we report ionization engineering,which endows polymer chains of hydrogels with electronegativity for impeding salt ions and activating water molecules,fundamentally overcoming the hydrogel salt-impeded challenge and dramatically expediting water evaporating in brine.The sodium dodecyl benzene sulfonate-modified carbon black is chosen as the solar absorbers.The hydrogel reaches a ground-breaking evaporation rate of 2.9 kg m−2 h−1 in 20 wt%brine with 95.6%efficiency under one sun irradiation,surpassing most of the reported literature.More notably,such a hydrogel-based evaporator enables extracting clean water from oversaturated salt solutions and maintains durability under different high-strength deformation or a 15-day continuous operation.Meantime,on the basis of the cation selectivity induced by the electronegativity,we first propose an all-day system that evaporates during the day and generates salinity-gradient electricity using waste-evaporated brine at night,anticipating pioneer a new opportunity for all-day resource-generating systems in fields of freshwater and electricity.

The transcription factor ZmNAC84 increases maize salt tolerance by regulating ZmCAT1 expression

Salt stress severely affects plant growth and yield.The transcription factor NAC plays a variety of important roles in plant abiotic stress,but we know relatively little about the specific molecular mechanisms of NAC in antioxidant defense.Here,our genetic studies reveal the positive regulation of salt tolerance in maize by the transcription factor ZmNAC84.Under salt stress,overexpression of ZmNAC84 in maize increased the expression of ZmCAT1,enhanced CAT activity,and consequently reduced H_(2)O_(2) accumulation,thereby improving salt stress tolerance in maize.Whereas RNA interference-mediated knockdown of ZmNAC84 produced the opposite effect.Subsequently,we found that ZmNAC84 directly binds to and regulates the expression of the ZmCAT1 promoter,and the hybridized material also demonstrated that ZmCAT1 is a downstream target gene of ZmNAC84.In addition,phenotypic and biochemical analyses indicated that ZmCAT1 positively regulated salt tolerance by regulating H_(2)O_(2) accumulation under salt stress.Taken together,these results reveal the function of ZmNAC84 in regulating ZmCAT1-mediated antioxidant defense in response to salt stress in plants.

Biomass-enhanced Janus sponge-like hydrogel with salt resistance and highstrength for efficient solar desalination

Interfacial solar-driven evaporation technology shows great potential in the field of industrial seawater desalination, and the development ofefficient and low-cost evaporation materials is key to achieving large-scale applications. Hydrogels are considered to be promising candidates;however, conventional hydrogel-based interfacial solar evaporators have difficulty in simultaneously meeting multiple requirements, including ahigh evaporation rate, salt resistance, and good mechanical properties. In this study, a Janus sponge-like hydrogel solar evaporator (CPAS) withexcellent comprehensive performance was successfully constructed. The introduction of biomass agar (AG) into the polyvinyl alcohol (PVA)hydrogel backbone reduced the enthalpy of water evaporation, optimized the pore structure, and improved the mechanical properties. Meanwhile, by introducing hydrophobic fumed nano-silica aerogel (SA) and a synergistic foaming-crosslinking process, the hydrogel spontaneouslyformed a Janus structure with a hydrophobic surface and hydrophilic bottom properties. Based on the reduction of the evaporation enthalpy andthe modulation of the pore structure, the CPAS evaporation rate reached 3.56 kg m^(-2) h^(-1) under one sun illumination. Most importantly, owingto the hydrophobic top surface and 3D-interconnected porous channels, the evaporator could work stably in high concentrations of salt-water(25 wt% NaCl), showing strong salt resistance. Efficient water evaporation, excellent salt resistance, scalable preparation processes, and low-costraw materials make CPAS extremely promising for practical applications.

Synergistic effects of carbon cycle metabolism and photosynthesis in Chinese cabbage under salt stress

Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and hormone metabolism, nutritional balances, and results in ion toxicity in plants. To better understand the mechanisms of salt-induced growth inhibition in Chinese cabbage, RNA-seq and physiological index determination were conducted to explore the impacts of salt stress on carbon cycle metabolism and photosynthesis in Chinese cabbage. Here, we found that the number of thylakoids and grana lamellae and the content of starch granules and chlorophyll in the leaves of Chinese cabbage under salt stress showed a time-dependent response, first increasing and then decreasing. Chinese cabbage increased the transcript levels of genes related to the photosynthetic apparatus and carbon metabolism under salt stress, probably in an attempt to alleviate damage to the photosynthetic system and enhance CO_(2) fixation and energy metabolism. The transcription of genes related to starch and sucrose synthesis and degradation were also enhanced;this might have been an attempt to maintain intracellular osmotic pressure by increasing soluble sugar concentrations. Soluble sugars could also be used as potential reactive oxygen species(ROS) scavengers, in concert with peroxidase(POD)enzymes, to eliminate ROS that accumulate during metabolic processes. Our study characterizes the synergistic response network of carbon metabolism and photosynthesis under salt stress.

Risk assessment of oil and gas investment environment in countries along the Belt and Road Initiative

With the implementation of the Belt and Road Initiative, China is deepening its cooperation in oil and gas resources with countries along the Initiative. In order to better mitigate risks and enhance the safety of investments, it is of significant importance to research the oil and gas investment environment in these countries for China's overseas investment macro-layout. This paper proposes an indicator system including 27 indicators from 6 dimensions. On this basis, game theory models combined with global entropy method and analytic hierarchy process are applied to determine the combined weights, and the TOPSIS-GRA model is utilized to assess the risks of oil and gas investment in 76 countries along the Initiative from 2014 to 2021. Finally, the GM(1,1) model is employed to predict risk values for 2022-2025. In conclusion, oil and gas resources and political factors have the greatest impact on investment environment risk, and 12 countries with greater investment potential are selected through cluster analysis in conjunction with the predicted results. The research findings may provide scientific decisionmaking recommendations for the Chinese government and oil enterprises to strengthen oil and gas investment cooperation with countries along the Belt and Road Initiative.

Nanopriming with selenium doped carbon dots improved rapeseed germination and seedling salt tolerance

Soil salinity is a big environmental issue affecting crop production.Although seed nanopriming has been widely used to improve seed germination and seedling growth under salinity,our knowledge about the underlying mechanisms is still insufficient.Herein,we newly synthesized selenium-doped carbon dots nanoparticles coated with poly acrylic acid(poly acrylic acid coated selenium doped carbon dots,PAA@Se-CDs)and used it to prime seeds of rapeseeds.The TEM(transmission electron microscope)size and zeta potential of PAA@Se-CDs are 3.8±0.2 nm and-30 mV,respectively.After 8 h priming,the PAA@Se-CDs nanoparticles were detected in the seed compartments(seed coat,cotyledon,and radicle),while no such signals were detected in the NNP(no nanoparticle control)group(SeO_2 was used as the NNP).Nanopriming with PAA@Se-CDs nanoparticles increased rapeseeds germination(20%)and seedling fresh weight(161%)under saline conditions compared to NNP control.PAA@Se-CDs nanopriming significantly enhanced endo-β-mannanase activities(255%increase,21.55μmol h^(-1)g^(-1)vs.6.06μmol h^(-1)g^(-1),at DAS 1(DAS,days after sowing)),total soluble sugar(33.63 mg g^(-1)FW(fresh weight)vs.20.23 mg g^(-1)FW)and protein contents(1.96μg g^(-1)FW vs.1.0μg g^(-1)FW)to support the growth of germinating seedlings of rapeseeds under salt stress,in comparison with NNP co ntrol.The respiration rate and ATP content were increased by 76%and 607%,respectively.The oxidative damage of salinity due to the overaccumulation of reactive oxygen species(ROS)was alleviated by PAA@Se-CDs nanopriming by increasing the antioxidant enzyme activities(SOD(superoxide dismutase),POD(peroxidase),and CAT(catalase)).Another mechanism behind PAA@Se-CDs nanopriming improving rapeseeds salt tolerance at seedling stage was reducing sodium(Na^(+))accumulation and improving potassium(K^(+))retention,hence increasing the K^(+)/Na^(+)ratio under saline conditions.Overall,our results not only showed that seed nanopriming with PAA@Se-CDs could be a good approach to improve salt tolerance,but also add more knowledge to the mechanism behind nanopriming-improved plant salt tolerance at germination and early seedling growth stage.

A Brief Discussion on the Development of the Silk-Weaving Industry Along the “Southern Silk Road” in Yunnan

Ancient Yunnan was one of the most significant regions along China’s ancient“Southern Silk Road.”During the Nanzhao period(738–902)of the late Tang Dynasty,Yunnan’s silk-weaving industry underwent a qualitative leap as skilled silk craftsmen from the Bashu area migrated to Yunnan and introduced mulberry planting,silkworm breeding,and advanced silk-weaving techniques from Sichuan to the region.Consequently,people in Yunnan gradually acquired expertise in brocade weaving and embroidery.Many even mastered complex silk-weaving techniques.The development and progress of the silk-weaving industry in the ancient Yunnan region were intricately linked to the economic function and value of silk as both a commodity and currency along the“Southern Silk Road.”The local government in ancient Yunnan was greatly motivated by the economic interests brought by the development of silk-related industries and recognized the significance of developing the local silk industry.They even initiated a campaign to capture skilled silk craftsmen from Sichuan,aiming to foster the growth of the silk-weaving industry in Yunnan.After years of dedicated efforts from the local government in ancient Yunnan,the region emerged as a significant hub for silk production along China’s ancient“Southern Silk Road.”Despite the devastation caused by the wars in other parts of the country,Yunnan’s silk industry continued to thrive and provide ample silk products to sustain trade along this renowned route.In the contemporary era,amidst the decline of the silk-weaving industry in eastern China,Yunnan has proposed an industrial development strategy known as“relocating the silk-weaving industry from east to west.”This involves introducing advanced silk production techniques from the eastern regions into Yunnan to enhance and enrich its local silk industry,thereby establishing it as a traditional national sector and securing a competitive position within the global silk market.The historical experience of Yunnan’s silk industry demonstrated that economic development opportunities can only be seized through proactive endeavors rather than passive anticipation.The modern Yunnan silk industry,which upholds its historical traditions,continues to actively engage in international high-end technical cooperation,thus ensuring the enduring vitality of the ancient“Southern Silk Road.”

基于ROAD的租车订购系统业务流程建模

基于业务架构为中心的企业架构开发思路,采用开放组架构框架(TOGAF)业务架构ACF元模型的划分,提出了一种ROAD架构迭代建模方法。针对目前ROAD元架构中业务活动模型采用IDEF0的活动模型导致的面向场景业务能力的不足,文中通过使用BPMN模型来覆盖IDEF0的活动建模,避免在描述复杂的业务过程时可能存在的局限性,并且更好地捕捉和表示所有的细节和关系,不会使得模型过于抽象。从而实现面向场景的系统建模和管理,提高业务架构设计的效率和精度。最后,文章中以旅游出行租车订购系统为例的方法,采用ROAD元架构方法建立场景化业务架构模型组,并对其进行讨论和改进,为现有架构体系提供一种面向场景的扩展思路。

The coordinated evolution of ecological environment,public service,and tourism economy along the Silk Road Economic Belt,using the Dual-Carbon Targets

Carbon peak and carbon neutrality(dual-carbon)are important targets for the international response to climate change.The Silk Road Economic Belt is a strategic resource region and is important for future ecological environment and tourism development.Based on the“dual-carbon”targets,the Single index quantification,Multiple index synthesis,and Poly-criteria integration evaluation model were used in this study to measure the coordinated development index of the ecological environment,public service,and tourism economy along the Silk Road Economic Belt and to analyze its spatial and temporal evolution.Further,it explores the dynamic evolution and development trend of the three systems using the Kernel Density and Grey Markov Prediction Model.The results show that the coordinated development index along this region needs to be improved during the study period.Furthermore,the coordinated development index of the Southwest region is relatively higher than that of the Northwest region.From the development trend of the three systems,all of them develop in a stable manner;however,the tourism economy system is easily affected by external disturbances.The coordinated development index of the three systems changes dynamically and tends to be in a good state of coordination.There is a certain spatial and temporal heterogeneity.The gravity center of the coordinated development index has been in the Southwest region.During the forecast period,the coordinated development index along this region will improve significantly,while insufficient and unbalanced development will continue.

Genome-Wide Exploration of the Grape GLR Gene Family and Differential Responses of VvGLR3.1 and VvGLR3.2 to Low Temperature and Salt Stress

Grapes,one of the oldest tree species globally,are rich in vitamins.However,environmental conditions such as low temperature and soil salinization significantly affect grape yield and quality.The glutamate receptor(GLR)family,comprising highly conserved ligand-gated ion channels,regulates plant growth and development in response to stress.In this study,11 members of the VvGLR gene family in grapes were identified using whole-genome sequence analysis.Bioinformatic methods were employed to analyze the basic physical and chemical properties,phylogenetic trees,conserved domains,motifs,expression patterns,and evolutionary relationships.Phylogenetic and collinear analyses revealed that the VvGLRs were divided into three subgroups,showing the high conservation of the grape GLR family.These members exhibited 2 glutamate receptor binding regions(GABAb and GluR)and 3-4 transmembrane regions(M1,M2,M3,and M4).Real-time quantitative PCR analysis demonstrated the sensitivity of all VvGLRs to low temperature and salt stress.Subsequent localization studies in Nicotiana tabacum verified that VvGLR3.1 and VvGLR3.2 proteins were located on the cell membrane and cell nucleus.Additionally,yeast transformation experiments confirmed the functionality of VvGLR3.1 and VvGLR3.2 in response to low temperature and salt stress.Thesefindings highlight the significant role of the GLR family,a highly conserved group of ion channels,in enhancing grape stress resistance.This study offers new insights into the grape GLR gene family,providing fundamental knowledge for further functional analysis and breeding of stress-resistant grapevines.

3D Road Network Modeling and Road Structure Recognition in Internet of Vehicles

Internet of Vehicles (IoV) is a new system that enables individual vehicles to connect with nearby vehicles,people, transportation infrastructure, and networks, thereby realizing amore intelligent and efficient transportationsystem. The movement of vehicles and the three-dimensional (3D) nature of the road network cause the topologicalstructure of IoV to have the high space and time complexity.Network modeling and structure recognition for 3Droads can benefit the description of topological changes for IoV. This paper proposes a 3Dgeneral roadmodel basedon discrete points of roads obtained from GIS. First, the constraints imposed by 3D roads on moving vehicles areanalyzed. Then the effects of road curvature radius (Ra), longitudinal slope (Slo), and length (Len) on speed andacceleration are studied. Finally, a general 3D road network model based on road section features is established.This paper also presents intersection and road section recognition methods based on the structural features ofthe 3D road network model and the road features. Real GIS data from a specific region of Beijing is adopted tocreate the simulation scenario, and the simulation results validate the general 3D road network model and therecognitionmethod. Therefore, thiswork makes contributions to the field of intelligent transportation by providinga comprehensive approach tomodeling the 3Droad network and its topological changes in achieving efficient trafficflowand improved road safety.

Nitrogen application regulates antioxidant capacity and flavonoid metabolism,especially quercetin,in grape seedlings under salt stress

Salt stress is a typical abiotic stress in plants that causes slow growth,stunting,and reduced yield and fruit quality.Fertilization is necessary to ensure proper crop growth.However,the effect of fertilization on salt tolerance in grapevine is unclear.In this study,we investigated the effect of nitrogen fertilizer(0.01 and 0.1 mol L^(-1)NH_(4)NO_(3))application on the salt(200 mmol L^(-1)NaCl)tolerance of grapevine based on physiological indices,and transcriptomic and metabolomic analyses.The results revealed that 0.01 mol L^(-1)NH_(4)NO_(3) supplementation significantly reduced the accumulation of superoxide anion(O_(2)^(-)·),enhanced the activities of superoxide dismutase(SOD)and peroxidase(POD),and improved the levels of ascorbic acid(AsA)and glutathione(GSH)in grape leaves compared to salt treatment alone.Specifically,joint transcriptome and metabolome analyses showed that the differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)were significantly enriched in the flavonoid biosynthesis pathway(ko00941)and the flavone and flavonol biosynthesis pathway(ko00944).In particular,the relative content of quercetin(C00389)was markedly regulated by salt and nitrogen.Further analysis revealed that exogenous foliar application of quercetin improved the SOD and POD activities,increased the AsA and GSH contents,and reduced the H_(2)O_(2) and O_(2)^(-)·contents.Meanwhile,10 hub DEGs,which had high Pearson correlations(R^(2)>0.9)with quercetin,were repressed by nitrogen.In conclusion,all the results indicated that moderate nitrogen and quercetin application under salt stress enhanced the antioxidant system defense response,thus providing a new perspective for improving salt tolerance in grapes.

The source of lithium in Lakkor Co Salt Lake on Qinghai-Tibet Plateau,China:evidence from hydrochemical characteristics and boron isotope

The availability of lithium resources is of great significance for the development of modern technologies,as well as for civil and military industries.The Qinghai-Tibet Plateau is a region known for its abundance of lithium-rich salt lakes.However,the specific origin of lithium in these lakes is still unknown,which hinders the advancement of the lithium resource business in this region.To research this issue,this study involved the collection of 20 samples from Lakkor Co Salt Lake on Qinghai-Tibet Plateau,encompassing samples of surface brine,cold springs,fresh lakes,and recharge rivers.The composition of anions and cations in these samples was determined.Furthermore,the analysis extensivelyutilizedthePiperthree-linediagram,Gibbs model,and ion proportion coefficient.The findings of this study indicate that as the moves from the recharge water system to salt lake,there is a transition in water type from strong carbonate to moderate carbonate and weak carbonate,as well as Na sulfate.This research based on a similar source of both lithium and boron,utilized ion correlation analysis and boron isotope study in the Lakkor Co area,and analyzed the source and transporting process of lithium.The main origin of lithium in Lakkor Co is the dissolution of lithiumrich rocks,recharge water systems,and deep hydrothermal fluids.These findings are highly significant in enhancing the foundational data of lithium-rich brine resources in the Qinghai-Tibet Plateau and are beneficial for assessing the future development of such deposits.

Automation in road distress detection,diagnosis and treatment

Road transportation plays a crucial role in society and daily life,as the functioning and durability of roads can significantly impact a nation's economic development.In the whole life cycle of the road,the emergence of disease is unavoidable,so it is necessary to adopt relevant technical means to deal with the disease.This study comprehensively reviews the advancements in computer vision,artificial intelligence,and mobile robotics in the road domain and examines their progress and applications in road detection,diagnosis,and treatment,especially asphalt roads.Specifically,it analyzes the research progress in detecting and diagnosing surface and internal road distress and related techniques and algorithms are compared.In addition,also introduces various road gover-nance technologies,including automated repairs,intelligent construction,and path planning for crack sealing.Despite their proven effectiveness in detecting road distress,analyzing diagnoses,and planning maintenance,these technologies still confront challenges in data collection,parameter optimization,model portability,system accuracy,robustness,and real-time performance.Consequently,the integration of multidisciplinary technologies is imperative to enable the development of an integrated approach that includes road detection,diagnosis,and treatment.This paper addresses the challenges of precise defect detection,condition assessment,and unmanned construction.At the same time,the efficiency of labor liberation and road maintenance is achieved,and the automation level of the road engineering industry is improved.

Growth kinetics of titanium carbide coating by molten salt synthesis process on graphite sheet surface

The synthesis of carbide coatings on graphite substrates using molten salt synthesis(MSS),has garnered significant interest due to its cost-effective nature.This study investigates the reaction process and growth kinetics involved in MSS,shedding light on key aspects of the process.The involvement of Ti powder through liquid-phase mass transfer is revealed,where the diffusion distance and quantity of Ti powder play a crucial role in determining the reaction rate by influencing the C content gradient on both sides of the carbide.Furthermore,the growth kinetics of the carbide coating are predominantly governed by the diffusion behavior of C within the carbide layer,rather than the chemical reaction rate.To analyze the kinetics,the thickness of the carbide layer is measured with respect to heat treatment time and temperature,unveiling a parabolic relationship within the temperature range of 700-1300℃.The estimated activation energy for the reaction is determined to be 179283 J·mol^(-1).These findings offer valuable insights into the synthesis of carbide coatings via MSS,facilitating their optimization and enhancing our understanding of their growth mechanisms and properties for various applications.

Wild soybean(Glycine soja)transcription factor GsWRKY40 plays positive roles in plant salt tolerance

Wild soybean(Glycine soja),a relative of cultivated soybean,shows high adaptability to adverse environmental conditions.We identified and characterized a wild soybean transcription factor gene,GsWRKY40,that promotes plant salt stress.GsWRKY40 was highly expressed in wild soybean roots and was up-regulated by salt treatment.GsWRKY40 was localized in nucleus and demonstrated DNA-binding activities but without transcriptional activation.Mutation and overexpression of GsWRKY40 altered salt tolerance of Arabidopsis plants.To understand the molecular mechanism of GsWRKY40 in regulating plant salt resistance,we screened a cDNA library and identified a GsWRKY40 interacting protein GsbHLH92 by using yeast two-hybrid approach.The physical interaction of GsWRKY40 and GsbHLH92 was confirmed by co-immunoprecipitation(co-IP),GST pull-down,and bimolecular fluorescence complementation(BiFC)techniques.Intriguingly,co-overexpression of GsWRKY40 and GsbHLH92 resulted in higher salt tolerance and lower ROS levels than overexpression of GsWRKY40 or GsbHLH92 in composite soybean plants,suggesting that GsWRKY40 and GsbHLH92 may synergistically regulate plant salt resistance through inhibiting ROS production.qRT-PCR data indicated that the expression level of GmSPOD1 gene encoding peroxidase was cooperatively regulated by GsWRKY40 and GsbHLH92,which was confirmed by using a dual luciferase report system and yeast one-hybrid experiment.Our study reveals a pathway that GsWRKY40 and GsbHLH92 collaboratively up-regulate plant salt resistance through impeding GmSPOD1 expression and reducing ROS levels,providing a novel perspective on the regulatory mechanisms underlying plant tolerance to abiotic stresses.