仙鹿活骨丸对激素性股骨头坏死细胞凋亡影响的实验研究

目的从微观结构变化考察仙鹿活骨丸对激素性股骨头坏死细胞凋亡影响,为临床应用提供参考。方法给大鼠臀肌注射醋酸强的松龙造成激素型股骨头坏死模型,通过原位细胞凋亡检测、光镜和电镜考察仙鹿活骨丸对激素性股骨头坏死细胞凋亡影响。结果原位细胞凋亡检测分析,发现模型大鼠给仙鹿活骨丸后细胞凋亡数量明显减少;光镜观察可以发现,仙鹿活骨丸可以减少空缺骨陷窝数,降低股骨头组织坏死程度。电镜观察可以发现,仙鹿活骨丸治疗后的骨细胞细胞核染色质和染色体相对均匀,线粒体、高尔基体、内质网等细胞器增多,而糖原溶解与脂肪空泡均较模型对照组减少,因而可提高成骨细胞骨形成的功能,有助于股骨头缺血性坏死修复过程。结论仙鹿活骨丸可以减少激素性股骨头坏死细胞凋亡,改善坏死部位的组织形态学,治疗股骨头坏死。

仙鹿活骨丸对大鼠股骨头缺血性坏死的干预治疗作用

目的考察仙鹿活骨丸对大鼠股骨头缺血性坏死的干预治疗作用,并探讨其作用机制。方法给大鼠通过灌胃维甲酸造成股骨头缺血性坏死模型,通过骨密度观察、血液流变学检测和光镜观察仙鹿活骨丸治疗股骨头坏死的疗效。结果仙鹿活骨丸能明显改善股骨头部位的骨密度水平和血液流变学,改善软骨表面凹陷,降低软骨细胞变性坏死度。结论仙鹿活骨丸具有活血化瘀作用,可以降低股骨头缺血性坏死程度,改善坏死部位的组织形态学以治疗股骨头坏死。

The Exploration of the Clinical Treatment of Chronic Atrophic Gastritis

Purpose:To explore the effect of the clinical treatment of chronic atrophic gastritis Methods:70 patients with chronic atrophic gastritis treated in our hospital from October 2017 to November 2018 were selected and randomly divided into an observation group and a control group with 35 patients in each group.The control group received standard triad treatment with gastroprokinetic drug orally.On the basis of the control group,patients in the observation group received Xianlu Pazhu Wan.The treatment efficiency,eradication rate of helicobacter pylori(H.pylori),incidence of adverse effects and length of stay were compared between the two groups.Results:Compared with the control group,patients in the observation group had higher treatment efficiency,higher H.pylori eradication rate,lower incidence of adverse effects,and shorter length of stay with statistical significance(P<0.05).Conclusion:In the clinical treatment of patients with chronic atrophic gastritis,adding Xianlu Pazhu Wan into the basic medication plan can have better treatment efficiency,reduce the incidence of adverse effects,and shorten the length of stay.Therefore,Xianlu Pazhu Wan should be promoted in the practice.

仙鹿活骨丸的中药毒理学研究

目的：检测仙鹿活骨丸的长期毒理。方法：连续给大鼠灌胃仙鹿活骨丸90天，测定大鼠体重、主要脏器质量指数、血常规、肝肾功能及心、肝、脾、肺、肾的病理改变。结果：仙鹿活骨丸的长期毒理性实验结果表明，连续给大鼠灌胃仙鹿活骨丸90天，对大鼠体重、主要脏器质量指数、血常规、肝肾功能无明显影响，心、肝、脾、肺、肾亦无明显病理改变。结论：表明该药毒性低微，可长期使用。

正交试验优选仙鹿活骨丸的水提工艺

目的:优选仙鹿活骨丸的水提工艺。方法:以芍药苷和盐酸小檗碱的得率为指标,采用正交试验考察加水量、提取时间及提取次数等因素的影响,综合评分法优选仙鹿活骨丸的水提工艺。结果:优选出的水提工艺为用8倍量水(第2次加6倍),回流提取2次,每次1h。结论:优选的提取工艺科学合理,稳定可行。