Effects of Heterologously Overexpressing PIP5K-Family Genes in Arabidopsis on Inflorescence Development

Castor is one of the top 10 oil crops in the world and has extremely valuable uses.Castor inflorescences directly affect yield,so the study of inflorescence development is very important in increasing castor yield.Our previous studies have shown that the PIP5K gene family(PIP5Ks)is associated with inflorescence development.In this study,to determine the function of each PIP5K gene in castor,a female Lm-type castor line,aLmAB2,was used to determine the relative expression levels of the PIP5Ks in castor inflorescences.Six PIP5K genes were heterologously overexpressed in Arabidopsis thaliana,the relative expression of each gene and the effect on plants was determined in A.thaliana,and the relationships among the PIP5Ks in castor were inferred.The expression levels of the PIP5Ks in the female Lm-type castor line aLmAB2 were analyzed.The relative expression levels of the PIP5K9 and PIP5K11 genes were high(p<0.05)in isofemale inflorescences,and those of PIP5K1,PIP5K2,PIP5K6,and PIP5K8 were high(p<0.05)in female inflorescences but low(p<0.05)in bisexual inflorescences.The PIP5Ks were heterologously overexpressed in A.thaliana,and T3-generation plants with stable genetic resistance,i.e.,AT-PIP5K^(+)plants(AT-PIP5K1^(+),AT-PIP5K2^(+),AT-PIP5K6^(+),AT-PIP5K8^(+),AT-PIP5K9^(+),and ATPIP5K11^(+) plants),were obtained.Biological tests of the AT-PIP5K+plants showed that the growth of the main stem was significantly delayed in AT-PIP5K+plants compared with Columbia wild-type(WT)A.thaliana plants;the PIP5K1 and PIP5K2 genes promoted lateral stem growth and flower and silique development;and the PIP5K6,PIP5K8,PIP5K9 and PIP5K11 genes inhibited lateral stem growth and flower and silique development.The correlations among PIP5Ks in castor suggest that there may be a synergistic relationship among PIP5K1,PIP5K2,and PIP5K6 in castor inflorescences,and PIP5K8,PIP5K9,and PIP5K11 are complementary to the other three genes.

Research on the Development Strategy of Smart Tourism in Hainan Under the Background of Big Data

Hainan is a major tourist province.It is urgent to promote the transformation and upgrading of Hainan’s tourism industry from a traditional service industry to a modern service industry by means of informatization.Smart tourism is a brand-new tourism form and operation mode of tourism transformation and upgrading.Integrating big data technology will make smart tourism more accurate in three aspects:tourism management,tourism service,and tourism marketing,and further enhance the satisfaction of the tourism experience.This paper studies the development status of smart tourism in Hainan,deeply summarizes its existing problems and causes,and puts forward the development strategy of smart tourism in Hainan to promote the healthy development of the tourism industry in Hainan.

Exploration of Teaching Development Strategies for Double-Qualified Teachers in University- Affiliated Hospitals Based on Role Conflict Theory

This study draws on the classic theories and research achievements of university teacher development,and from the perspective of role conflict in social psychology,proposes policy recommendations for the development of clinical teachers in medical colleges,including following different stages of teacher development and designing teaching development strategies at different levels;designing the content and form of teaching development activities to meet the temporal and spatial needs of clinical teachers;and building an academic community for clinical teachers to promote the creation of teaching development behaviors.

Analysis of Innovative Paths for Enterprise Marketing Management Development Strategy under the Background of New Media

From the perspective of enterprise marketing strategy planning,innovative research should be conducted based on the current trends in new media.The findings should be applied to practical activities within enterprises to achieve innovation in marketing strategy decision-making in the new media environment.Enterprises should adopt advanced communication transmission technologies and big data processing capabilities to effectively leverage the hidden value and promotional planning advantages of network media platforms.This approach can enhance customer loyalty and increase demand for new products.Enterprises should integrate into the continuously evolving new media landscape,explore their potential business characteristics,and innovate promotional methods.By effectively combining product information with digital media content,companies can ultimately achieve significant increases in corporate interests.

Development Strategy of Sichuan Sports Museum under the Background of Cultural and Tourism Integration

Through conducting a SWOT analysis of the Chengdu Sports Institute Museum,and utilizing the 4M management,5W display,and 4P marketing theoretical models,this study explores effective strategies suitable for Sichuan sports museums in terms of functionality,display,and marketing.

FE65:a hub for neurodevelopment

FE65,initially identified as a binding partner of amyloid precursor protein(APP),is an adaptor protein enriched in the brain and regulated during development.FE65 belongs to the FE65 protein family.This family is comprised of three members,FE65,FE65 like-1(FE65L1),and FE65 like-2(FE65L2).

AI-Powered Innovations in High-Tech Research and Development: From Theory to Practice

This comparative review explores the dynamic and evolving landscape of artificial intelligence(AI)-powered innovations within high-tech research and development(R&D).It delves into both theoreticalmodels and practical applications across a broad range of industries,including biotechnology,automotive,aerospace,and telecom-munications.By examining critical advancements in AI algorithms,machine learning,deep learning models,simulations,and predictive analytics,the review underscores the transformative role AI has played in advancing theoretical research and shaping cutting-edge technologies.The review integrates both qualitative and quantitative data derived from academic studies,industry reports,and real-world case studies to showcase the tangible impacts of AI on product innovation,process optimization,and strategic decision-making.Notably,it discusses the challenges of integrating AI within complex industrial systems,such as ethical concerns,technical limitations,and the need for regulatory oversight.The findings reveal a mixed landscape where AI has significantly accelerated R&D processes,reduced costs,and enabled more precise simulations and predictions,but also highlighted gaps in knowledge transfer,skills adaptation,and cross-industry standardization.By bridging the gap between AI theory and practice,the review offers insights into the effectiveness,successes,and obstacles faced by organizations as they implement AI-driven solutions.Concluding with a forward-looking perspective,the review identifies emerging trends,future challenges,and promising opportunities inAI-poweredR&D,such as the rise of autonomous systems,AI-driven drug discovery,and sustainable energy solutions.It offers a holistic understanding of how AI is shaping the future of technological innovation and provides actionable insights for researchers,engineers,and policymakers involved in high-tech Research and Development(R&D).

Functional Studies of Castor(Ricinus communis L.)PLC Family Genes in Arabidopsis Inflorescence Development

Castor(Ricinus communis L.)is one of the top 10 oil crops in the world,and inflorescence is a trait that directly affects its yield.Phospholipase C(PLCs)is involved in many plant activities and metabolic processes.To study the functions of PLC family genes in the regulation of the inflorescence development of the female line of Lm-type castor aLmAB2,we determined the expression levels of six PLC family genes of three types of inflorescences of aLmAB2(isofemale line,female line,bisexual line)at different developmental stages.The results showed that the 6 genes of the castor PLC family had relative expression levels at different developmental stages of the three types of inflorescences.The subcellular location of all six protein products was the cell membrane.The six genes were heterologously overexpressed in Arabidopsis thaliana to obtain the T3 generation-resistant Arabidopsis thaliana plants.The results showed that the overexpression of six genes significantly promoted the maturation of Arabidopsis thaliana,the growth of lateral moss,and the development of flowers and pods,but the development of basal leaves and stem leaves of Arabidopsis thaliana was significantly inhibited.According to homology analysis,it is speculated that PLC2,PLC2M,PLC2N,PLC4,PLC4X2,and PLC6 genes have the same regulatory function.

Challenges to establishing and maintaining kidney transplantation programs in developing countries:What are the coping strategies?

Kidney transplantation(KT)is the optimal form of renal replacement therapy for patients with end-stage renal diseases.However,this health service is not available to all patients,especially in developing countries.The deceased donor KT programs are mostly absent,and the living donor KT centers are scarce.Single-center studies presenting experiences from developing countries usually report a variety of challenges.This review addresses these challenges and the opposing strategies by reviewing the single-center experiences of developing countries.The financial challenges hamper the infrastructural and material availability,coverage of transplant costs,and qualification of medical personnel.The sociocultural challenges influence organ donation,equity of beneficence,and regular follow-up work.Low interests and motives for transplantation may result from high medicolegal responsibilities in KT practice,intense potential psychosocial burdens,complex qualification protocols,and low productivity or compensation for KT practice.Low medical literacy about KT advantages is prevalent among clinicians,patients,and the public.The inefficient organizational and regulatory oversight is translated into inefficient healthcare systems,absent national KT programs and registries,uncoordinated job descriptions and qualification protocols,uncoordinated on-site investigations with regulatory constraints,and the prevalence of commercial KT practices.These challenges resulted in noticeable differences between KT services in developed and developing countries.The coping strategies can be summarized in two main mechanisms:The first mechanism is maximizing the available resources by increasing the rates of living kidney donation,promoting the expertise of medical personnel,reducing material consumption,and supporting the establishment and maintenance of KT programs.The latter warrants the expansion of the public sector and the elimination of non-ethical KT practices.The second mechanism is recruiting external resources,including financial,experience,and training agreements.

Research on strategic risk identification method of equipment system development based on system dynamics

Strategic management of equipment system develop-ment must attach importance to effective strategic risk manage-ment.Aiming at the identification of strategic risk of equipment system development,firstly,the source of strategic risk of equip-ment system development is analyzed and classified.Based on this,a causal loop diagram of strategic risk of equipment sys-tem development based on system dynamics is established.The system dynamics analysis software Vensim PLE is used to carry out the risk influencing factors analysis,risk consequences ana-lysis,risk feedback loop identification and corresponding pre-control measures,and achieves a good risk identification effect.

Analysis of the Potential for Tourism Development on Semau Island(Case Study at Liman Beach)

Semau Island has enormous potential to become a tourist destination in East Nusa Tenggara.Tourism potential is a variety of resources found in a particular area that can be developed into a tourist attraction.The potential of this island is in the form of natural potential,animal husbandry,maritime culture,and agriculture.This research aims to analyze and classify the level of potential of tourist attractions on Semau Island in improving the regional economy at tourist attractions on Semau Island.The data used is the result of direct interviews with respondents,namely village heads,tourist attraction managers,and local communities.The analytical method used is descriptive qualitative with the SWOT analysis method.The research results concluded that the tourist attraction on Semau Island has the potential to be developed as a superior tourist attraction in Kupang Regency.Strategies or steps that can be taken by managers or local governments to increase tourism development in the form of standard tourism facilities,improving the quality of human resources,and improving infrastructure.

Early Intervention Facilitates Neuropsychological Development in Children with Autism and Attention Deficit Hyperactivity Disorder

Objective:This study aims to investigate the impact of early intervention on neuropsychological development in children with autism and attention deficit hyperactivity disorder(ADHD),providing effective intervention strategies for clinical practice.Methods:A total of 130 children with autism and ADHD who visited the hospital between June 2023 and June 2024 were selected as study subjects and randomly divided into an intervention group and a control group,with 65 children in each group.The intervention group received a one-year early comprehensive intervention,including behavioral therapy,cognitive training,and family guidance,while the control group only received routine medical care.The neuropsychological development assessment scale was used to evaluate both groups before and after the intervention to compare changes in their neuropsychological development levels.Results:Children in the intervention group showed significant improvements in cognitive function,social skills,language ability,and attention concentration,with an average improvement score of 23.5 points.Children in the control group did not show significant improvements in these areas,with an average improvement score of only 5.8 points.The difference between the two groups was statistically significant(P<0.05).Conclusion:Early comprehensive intervention has a significant promoting effect on the neuropsychological development of children with autism and ADHD.Targeted behavioral therapy,cognitive training,and family guidance can effectively enhance children’s cognitive,social,language,and attention abilities,laying a solid foundation for their future overall development.Therefore,it is recommended to actively promote and apply early intervention strategies in clinical practice.

Connotation and Strategies of High-quality Development of Farmers' Cooperatives in Ethnic Minority Areas : A Case Study of Guizhou Province

During the "14^(th) Five-Year Plan" period, consolidating the achievements of poverty alleviation, promoting rural revitalization and the high-quality development of farmers' cooperatives will overlap at the same time in the spatial field of ethnic minority areas, and they have consistency in content and commonality in value. The high-quality development of farmers' cooperatives in ethnic minority areas is an important organizational way to realize the modernization of agricultural industry, and it is also the key to consolidating the achievements of poverty alleviation and promoting rural revitalization at this stage. Because there is a gap between ethnic minority areas and developed areas in key factors of production such as capital, market, labor and technology, the development of farmers' cooperatives in ethnic minority areas faces more difficulties. This paper takes the development status and outstanding problems of farmers' cooperatives in Guizhou Province as the research object, discusses the connotation and significance of the high-quality development of farmers' cooperatives, and puts forward some strategies and suggestions on the governance of hollowing cooperatives, the allocation of key production factors, and the improvement of the quality of cooperatives.

Development Strategy of Urban Cultural Scene to Promote the Upgrading of Regional Cultural Consumption in Shaanxi

Regional cultural patterns and characteristics play a positive role in economic and social development.By planning and constructing cultural amenities and creating cultural scenes,the spatial quality and quality of life in a region can be enhanced,facilitating the expansion of cultural consumption.Shaanxi,with its rich historical and cultural resources,positions the capital city of Xi’an as a“world historical city”,boasting a vast number of cultural amenities represented by“cultural facilities”,“cultural activities”,“cultural experiences”,and“cultural services”.The development of urban cultural scene,with the aim of promoting the upgrading of regional cultural consumption in Shaanxi,requires comprehensive planning and a multifaceted approach,particularly in integrating provincial cultural scenes,clarifying the positioning of cultural scenes,innovating cultural scene experience projects,creating cultural scene intellectual property(IP),and empowering cultural scenes through the application of science and technology.

Publishers’Development Strategies in Response to Changes in National Reading-Analysis Based on the 16th to 20th National Reading Survey Report

Since 2014,nationwide reading has been included in the government work report for 10 consecutive times,and twice into the country’s five-year development plan.The importance of reading for individuals,society,and the country has become increasingly prominent.National reading has flourished,and throughout the 2018 to 2022 National Reading Survey Report,there have been welcome changes in national reading in China.This study compares and analyzes the relevant reading indicators of adult nationals from 2018 to 2022,and summarizes the five changes and new trends in national reading.That is,national reading awareness has increased,paper-book reading habits have returned,digital reading has become more diversified,the listening group has grown,and the voice of national reading activities has continued to rise.Accordingly,it provides relevant development strategies for publishers.

Current Status and Development Strategy of Mung Bean Production in Chongqing Municipality

[Objective] This paper presented the current status of mung bean produc- tion in Chongqing, and put forward the corresponding development strategies. [Meth- ods] By overviewing the status of mung bean production in Chongqing Municipality, the main problems, advantages of mung bean production in Chongqing were pre- sented, and based on corresponding development strategies were put forward.[Re- suits] Currently, the main problems of mung bean production in Chongqing area in- clude insufficient attention, serious variety degradation, lag in cultivation technology, low scientific research level. However, the region has strengths in growing mung beans which would have broad prospects by appropriate adjustment and planning. [Conclusion] This study laid the foundation for the development of mung bean pro- duction and promotion of economic development in Chongqing Municipality.

Current Status of Rice Production in Burundi and Its Development Strategies

Burundi, a landlocked country locked near the equator, is one of the least developed countries confirmed by the United Nations and the 60% financial revenue needs aid. Rice is the most important and extravagant food in Burundian consumption for no more than 5 kg on average per people by year. It＇s especially suitable to plant rice in Burundi because of the sufficient sunlight and rain. But the lack of new varieties, practical technology and modern service make it very difficult for rice production. According to this significant roll, the rice production has to be increased in order to satisfy the consumption needs which are now becoming higher and keep the food safe. This article mainly shows the rice ecological types, production facts and the major constraints of rice production in Burundi, and then look into the distance on how to solve the problems.

Current situation of Chinese forestry tactics and strategy of sustainable development

Based on the analysis of the current situation of Chinese forestry and a series of ecological, environmental, eco-nomic, and social problems, this paper emphasized on the importance of forests in social and economic development in China The author pointed out that sustainable development, particularly the sustainable development of forestry, is the inevitable choice to solve the problems of ecological environment and social economics. The objectives, criteria, and strategies of sus-taining forestry were also demonstrated and expounded, from the viewpoints of ecology, economics, and sociology.

Rural Tourism Development Strategies of Shaanxi Province in North China

Rural tourism development in Shaanxi Province is introduced:largely improved with a fast speed; large quantity of rural tourist sites covering a great range, a complete product system; more and more investment; new progress in the construction of management system and guarantee system; progress in the construction of new countryside. Development advantages and opportunities of rural tourism industry in Shaanxi Province are analyzed: rich humanistic, natural and agricultural resources are inborn advantages of its rural tourism development; excellent location and increasing GDP per capita provides more market opportunities for rural tourism development; prospering tourism industry provides an excellent exterior environment for rural tourism development; a complete traffic network facilitates rural tourism development. Then development strategies for the rural tourism industry of Shaanxi Province are given:government should be a guider, planning should be accelerated, layouts reasonably designed, rural tourism development concepts clarified; brands should be created to drive the overall development by using several prominent scenic spots and promote the scale-development of rural tourism industry; the core strategy is to launch concrete programs and promote rural tourism development; publicity should be enhanced to make the good reputation of rural tourism more popular; standard regulations should be made, special staff trained for rural tourism services and the system of "farm-stay" association improved.

Research Plan on the Strategic Development of High-value Eco-agriculture in the Middle-lower Reaches of Yangtze River

At present,high-value eco-agriculture research in China is till limited and controversial,theoretical innovation and practices on eco-agriculture should be conducted.The key problems which should be solved urgently on the development of high-value eco-agriculture in the middle-lower reaches of Yangtze River now including：the connotation and characteristics of high-value eco-agriculture,the construction of high-value eco-agriculture system and the policies of the development of high-value eco-agriculture.Therefore,the strategic development research on high-value eco-agriculture mainly includes the following aspects：development of agriculture modernization and theory and practices of eco-economics;theory of high-value eco-agriculture;practice of high-value eco-agriculture in the middle-lower reaches of Yangtze River;construction of high-value eco-agriculture in the middle-lower reaches of Yangtze River;development strategy and system innovation of high-value eco-agriculture in the lower and medium reaches of Yangtze River.