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Moral Perspective as Depicted in the Archetypes on Divine-Human Perspective of Dianshizhai Pictorial
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作者 REN Siying LIAO Chenxi 《Cultural and Religious Studies》 2024年第5期324-327,共4页
This article examines the archetypes related to the divine-human perspective in the Dianshizhai Pictorial and uses the basic theory of“Myth-Archetype Criticism”to analyze the concepts of moral perspective and the vi... This article examines the archetypes related to the divine-human perspective in the Dianshizhai Pictorial and uses the basic theory of“Myth-Archetype Criticism”to analyze the concepts of moral perspective and the view of cause and effect reflected in the text.It summarizes the interactive modes and various archetypes of cause and effect in three contexts:gods to humans,humans to gods,and humans to humans,explores the social reality and the relevant views of moral perspective and causality reflected in the illustrated magazine,and reveals the archetype foundation of the narrative of moral perspective retribution in Dianshizhai Pictorial in the three dimensions of psychology,literature,and society. 展开更多
关键词 Dianshizhai Pictorial divine-human perspective archetype study cause and effect retribution view of moral perspective
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Collaborative Cultivation of Ideological and Political Education and Undergraduate Management in Colleges and Universities Under the Guidance of Moral Education
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作者 Jianyuan Xie 《Journal of Contemporary Educational Research》 2024年第4期221-225,共5页
Guided by moral education,improving the ideological and moral quality of college students is an important goal of college education.To achieve this goal,it is necessary to strengthen ideological and political educatio... Guided by moral education,improving the ideological and moral quality of college students is an important goal of college education.To achieve this goal,it is necessary to strengthen ideological and political education and continuously improve the level of undergraduate management.This paper outlines the feasibility of collaborative cultivation of ideological and political education and undergraduate management in colleges and universities and analyzes its significance and the specific implementation strategies,with a view to providing valuable references for relevant colleges and universities to carry out educational work. 展开更多
关键词 moral education Colleges and universities Ideological and political education Undergraduate management
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Reading man皮瓣修复术治疗皮肤缺损的疗效观察
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作者 王鸣 严炜 +3 位作者 汤由之 朱喆辰 史京萍 马秀云 《临床皮肤科杂志》 CAS CSCD 北大核心 2024年第9期557-561,共5页
目的:探讨Reading man皮瓣修复术在全身不同部位皮肤缺损及其预防瘢痕增生的临床疗效。方法:回顾性分析2021年1月—2023年10月该科应用Reading man皮瓣修复术治疗全身不同部位皮肤软组织缺损的20例患者临床资料。结果:所有患者皮瓣均成... 目的:探讨Reading man皮瓣修复术在全身不同部位皮肤缺损及其预防瘢痕增生的临床疗效。方法:回顾性分析2021年1月—2023年10月该科应用Reading man皮瓣修复术治疗全身不同部位皮肤软组织缺损的20例患者临床资料。结果:所有患者皮瓣均成活。术后随访时间为3~24个月,所有皮瓣血运良好,色泽红润,质地柔软,外观适宜,局部皮肤张力较低,手术切口瘢痕增生不明显,切口周围软组织无明显形变,周围关节活动度正常。所有患者均恢复良好,疗效满意。结论:Reading man皮瓣特有的设计可以很好地解决全身多部位较大的皮肤软组织缺损,尤其在术中可以最大限度地减少正常皮肤的切除,术后外观、功能及皮肤瘢痕增生均有较满意的表现,且不存在自体皮移植带来的供皮区损伤。 展开更多
关键词 Reading man皮瓣 皮瓣血运 张力 瘢痕增生
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牛冠状病毒TaqMan荧光定量PCR检测方法的建立及应用
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作者 蒲鹏 李晨露 +2 位作者 张琪 吴发兴 许信刚 《动物医学进展》 北大核心 2024年第2期7-10,共4页
为建立牛冠状病毒TaqMan荧光定量PCR检测方法,根据GenBank收录的牛冠状病毒AKS-01株N基因序列(KU886219)保守区设计特异性引物和探针,构建重组质粒并进行反应条件优化、特异性试验、重复性试验以及敏感性试验,建立一种检测BCoV的TaqMan... 为建立牛冠状病毒TaqMan荧光定量PCR检测方法,根据GenBank收录的牛冠状病毒AKS-01株N基因序列(KU886219)保守区设计特异性引物和探针,构建重组质粒并进行反应条件优化、特异性试验、重复性试验以及敏感性试验,建立一种检测BCoV的TaqMan荧光定量PCR方法。结果显示,建立的牛冠状病毒TaqMan荧光定量PCR检测方法特异性、敏感性和重复性均良好。该方法BCoV重组质粒标准品在5.75×10^(7)~5.75×10^(3)copies/μL时与Ct值呈现良好线性关系,该方法对牛轮状病毒、牛传染性鼻气管炎病毒、牛病毒性腹泻病毒、牛副流感病毒3型均无交叉反应,特异性良好;该方法对BCoV重组质粒标准品最低检测限为5.75×10^(1)copies/μL;批内和批间重复性试验结果稳定,变异系数均小于2%。利用所建立的TaqMan荧光定量PCR方法对收集的132份样品进行检测,与常规PCR相比,两者符合率为96.21%,可为BCoV的临床检测和流行病学调查提供技术支持。 展开更多
关键词 牛冠状病毒 Taqman荧光定量PCR 检测方法
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An Exploration of Human Nature and a Challenge Against Conventional Morality of American South——On the Theme ofThe Adventures of Huckleberry Finn
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作者 胡辉平 《安徽职业技术学院学报》 2005年第4期52-55,共4页
文章通过对马克.吐温名著《哈克贝利.芬历险记》主题的分析,深刻探讨了马克.吐温对人的本性的探索和对美国南方传统道德的挑战,并进而提出了与现今社会紧密联系的人文关怀问题。
关键词 人性 挑战 传统道德 美国南方
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大口黑鲈弹状病毒Taq Man荧光定量PCR检测方法的建立及初步应用 被引量:1
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作者 孔明慧 梁红茹 +5 位作者 李宁求 林强 牛银杰 罗霞 马宝福 付小哲 《中国预防兽医学报》 CAS CSCD 北大核心 2024年第4期379-384,共6页
为建立快速、敏感的大口黑鲈弹状病毒(MSRV)流行株Taq Man荧光定量PCR (qPCR)方法,本研究采用NCBI分析MSRV流行株N基因的保守序列,设计特异性引物和Taq Man探针,以构建的并经PCR和测序鉴定正确的重组质粒标准品p MD18-MSRV-N为模板,通... 为建立快速、敏感的大口黑鲈弹状病毒(MSRV)流行株Taq Man荧光定量PCR (qPCR)方法,本研究采用NCBI分析MSRV流行株N基因的保守序列,设计特异性引物和Taq Man探针,以构建的并经PCR和测序鉴定正确的重组质粒标准品p MD18-MSRV-N为模板,通过优化各反应条件初步建立MSRV的Taq Man q PCR检测方法。结果显示,建立的Taq Man q PCR方法标准曲线的R2为0.9906,质粒标准品在6.79×10^(8)拷贝/μL~6.79×10^(2)拷贝/μL范围内与Ct值均存在较好的线性关系。采用该方法检测MSRV及相关病毒,评估该方法的特异性,结果显示,该方法可以特异性检测MSRV,而草鱼出血病病毒、神经坏死病毒、鲤春病毒血症病毒、大口黑鲈双RNA病毒、鳜蛙虹彩病毒、传染性脾肾坏死病毒、石斑鱼虹彩病毒、大口黑鲈蛙虹彩病毒等8种常见鱼类病毒检测结果均为阴性;采用建立的方法检测不同浓度的质粒标准品(6.79×10^(8)拷贝/μL~6.79×10^(1)拷贝/μL),评估该方法的敏感性,结果显示,该方法的检测限为6.79×10^(2)拷贝/μL,比文献中的常规PCR方法敏感1 000倍;以不同批次或同一批次提取的不同浓度的质粒标准品作为模板,分别利用该方法检测,评估该方法的重复性,结果显示,该方法批内和批间重复性试验的变异系数均小于2%。采用该方法和文献中的常规PCR同时检测71份临床发病的大口黑鲈、鳜鱼、鲮鱼组织样品,结果显示阳性率为16.9%(12/71),阴性率为83.1%(59/71),且阳性样品均是从发病大口黑鲈的组织样品中检测到,而发病的鳜鱼、鲮鱼组织样品中均未检测到该病毒;常规PCR方法的阳性率为9.9%(7/71),阴性率为90.1%(64/71),二者的总符合率为93.0%。本研究建立的MSRV Taq Man q PCR方法特异性强、敏感性高、重复性和准确性均较好,可用于大口黑鲈临床样品的检测,为MSRV的快速检测提供了可行的技术手段。 展开更多
关键词 大口黑鲈弹状病毒 Taq man荧光定量PCR N基因
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The Neural Mechanisms of Utility and Ethic in the Management Moral Decision Making 被引量:1
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作者 Zhiwen Tang Ying Lin 《Journal of Behavioral and Brain Science》 2015年第4期157-162,共6页
The study aimed at investigating the neural mechanism of business moral decision-making. 15 participants were presented with a set of 96 dilemmas in business situation. Participants were required to decide between opt... The study aimed at investigating the neural mechanism of business moral decision-making. 15 participants were presented with a set of 96 dilemmas in business situation. Participants were required to decide between option 1 (more utilitarian decision for self benefit) and 2 (ethical decision for fairness or institution). ERPs were recorded to a slide displaying the letter 1 and 2 when subjects were deciding between options. The selection rate was calculated after the experiment as the behavior data. The business moral dilemmas prompted more ethical option. A larger P260 component was elicited in the left frontal lobe area when participants were deciding to act ethically. On the other hand, decisions on utilitarian aroused weaker emotional experience, as supported by the shorter LPC and smaller P260 amplitude. 展开更多
关键词 management moral Decision Making EVENT-RELATED POTENTIALS NERVE
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鸡毒支原体 Taq Man实时荧光定量PCR检测方法的建立
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作者 孙宇 苗立中 +6 位作者 程立坤 赵家磊 赵修报 沈志强 王敬茹 李书光 余燕 《动物医学进展》 北大核心 2024年第4期64-68,共5页
为快速检测鸡毒支原体,根据鸡毒支原体的保守基因设计特异性引物和Taq Man探针,建立鸡毒支原体Taq Man实时荧光定量PCR方法并分析鸡毒支原体培养过程中颜色单位改变法(color change unit,CCU)和荧光定量PCR检测的相关性。结果显示,标准... 为快速检测鸡毒支原体,根据鸡毒支原体的保守基因设计特异性引物和Taq Man探针,建立鸡毒支原体Taq Man实时荧光定量PCR方法并分析鸡毒支原体培养过程中颜色单位改变法(color change unit,CCU)和荧光定量PCR检测的相关性。结果显示,标准曲线y=-3.646x+44.208,相关系数R^(2)=0.998,最低检测限度为1 copy/μL;与其他菌株等均无交叉反应;组内和组间变异系数均小于3%,表明该方法具有良好的稳定性和可重复性。用建立的实时荧光定量PCR检测方法对26份临床样品进行检测,该方法较普通PCR方法检出率更高。建立的荧光定量PCR与CCU显示鸡毒支原体在对数生长期时,两者具有一定的对应关系。表明建立了鸡毒支原体Taq Man探针实时荧光定量PCR检测方法,可实现对鸡毒支原体的快速检测,为鸡毒支原体感染的防控提供技术基础。 展开更多
关键词 鸡毒支原体 Taq man探针 荧光定量PCR
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牛支原体和丝状支原体丝状亚种双重TaqMan荧光定量PCR检测方法的建立与应用 被引量:3
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作者 马芷忻 武琪 +2 位作者 刘桐 辛九庆 徐青元 《中国预防兽医学报》 CAS CSCD 北大核心 2024年第3期262-268,共7页
牛传染性胸膜肺炎和牛支原体病均为重要的牛传染病,两者均可致牛出现呼吸系统症状,临床上难以区分。为建立鉴别诊断牛传染性胸膜肺炎和牛支原体病的双重Taq Man荧光定量PCR检测方法,本研究根据这两种病的病原丝状支原体丝状亚种和牛支... 牛传染性胸膜肺炎和牛支原体病均为重要的牛传染病,两者均可致牛出现呼吸系统症状,临床上难以区分。为建立鉴别诊断牛传染性胸膜肺炎和牛支原体病的双重Taq Man荧光定量PCR检测方法,本研究根据这两种病的病原丝状支原体丝状亚种和牛支原体的基因组保守区域(丝状支原体丝状亚种nt826-nt1742,牛支原体nt189-nt618)分别设计引物与探针,通过优化反应体系与反应条件,建立同时检测这两种病原的Taq Man荧光定量PCR检测方法。特异性试验结果显示,该方法仅对丝状支原体丝状亚种模拟样品和牛支原体检测结果为阳性,而巴氏杆菌、牛传染性鼻气管炎病毒、无乳支原体、山羊支原体山羊肺炎亚种、丝状支原体山羊亚种、关节炎支原体Leachii株检测结果均为阴性,特异性较强。分别以1.0×10~7拷贝/μL~1.0×10~1拷贝/μL的p EASY-Mmm和p EASY-Mb质粒标准品为模板,进行敏感性试验,结果显示,该方法对丝状支原体丝状亚种和牛支原体重组质粒标准品的检测限均为1.0×10~1拷贝/μL,敏感性较高。对不同浓度质粒标准品混合物的重复性试验结果显示,组内与组间重复性试验变异系数均小于2.5%,重复性较好。利用该方法对112份临床样品(104份鼻拭子、8份肺组织样品)检测,结果显示,丝状支原体丝状亚种检测结果和已发表PCR方法检测结果一致,均为阴性,而本实验建立的方法检测出4份牛支原体阳性样品,且经测序证实检出样品为真实阳性样品,而已发表的多重PCR方法检测该病原结果均为阴性。本研究建立了能够同时检测丝状支原体丝状亚种和牛支原体的双重Taq Man荧光定量PCR方法,其特异性强、敏感性高、重复性好,可用于各种临床样品的检测,为这两种病原的快速检测和流行病学调查提供了技术手段。 展开更多
关键词 牛传染性胸膜肺炎 牛支原体 Taqman荧光定量PCR
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羊贝氏柯克斯体TaqMan荧光定量PCR检测方法的建立及应用
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作者 张锐铮 于皓同 +3 位作者 王凯茸 张琪 张淑霞 许信刚 《动物医学进展》 北大核心 2024年第7期6-10,共5页
为建立检测贝氏柯克斯体(Coxiella burnetii)实时荧光定量PCR方法,根据GenBank上收录的C.burnetii RSA439株com1基因序列(CP040059.1)保守区设计特异性引物和探针,并构建重组质粒及优化反应条件,以期建立一种检测贝氏柯克斯体的Taq Man... 为建立检测贝氏柯克斯体(Coxiella burnetii)实时荧光定量PCR方法,根据GenBank上收录的C.burnetii RSA439株com1基因序列(CP040059.1)保守区设计特异性引物和探针,并构建重组质粒及优化反应条件,以期建立一种检测贝氏柯克斯体的Taq Man荧光定量PCR方法。结果显示,该方法所建立标准曲线线性关系良好;敏感性试验结果显示,最低检出限为2.45×10^(2) copies/μL,与PCR最低检出限为2.45×10^(4) copies/μL相比较,灵敏度提高了100倍;对羊支原体、羊流产衣原体、羊布鲁氏菌等均无交叉反应,批内和批间重复性试验结果稳定,变异系数均小于3%,说明特异性、重复性好;利用该方法对收集的126份样品进行检测,结果显示样品阳性率为9.52%,常规PCR检测阳性率为7.14%,符合率为90.47%。该方法可为贝氏柯克斯体临床上快速检测及防控提供技术支持。 展开更多
关键词 贝氏柯克斯 Taq man荧光定量PCR 检测方法
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猪肺炎支原体和猪鼻支原体TaqMan双重荧光PCR检测方法的建立及应用 被引量:1
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作者 周颖 赵硕 +10 位作者 覃国喜 梁龙华 肖婷 陈忠伟 卢冰霞 秦毅斌 林昌华 张胜斌 段群棚 胡庭俊 何颖 《动物医学进展》 北大核心 2024年第2期11-16,共6页
为建立一种可快捷鉴别检测猪肺炎支原体(Mhp)和猪鼻支原体(Mhr)的方法,针对Mhp的183基因和Mhr的p37基因保守片段,分别设计合成引物及TaqMan探针,优化反应条件,建立一种可同时检测Mhp和Mhr的TaqMan双重荧光PCR方法,并评价其特异性、敏感... 为建立一种可快捷鉴别检测猪肺炎支原体(Mhp)和猪鼻支原体(Mhr)的方法,针对Mhp的183基因和Mhr的p37基因保守片段,分别设计合成引物及TaqMan探针,优化反应条件,建立一种可同时检测Mhp和Mhr的TaqMan双重荧光PCR方法,并评价其特异性、敏感性和重复性。结果表明,该方法特异性强,检测其他常见猪呼吸系统病原不发生交叉反应;对标准品pMD18-T-Mhp-183、pMD18-T-Mhr-P37的最低检测限分别达45.2 copies/μL和29.7 copies/μL,比普通PCR检测灵敏度提高10^(3)~10^(4)倍;该方法检测结果的批内与批间变异系数均小于2%。用该方法检测145份广西自治区内临床样品,从中检出82份Mhp和9份Mhr阳性样品。该方法可用于临床样品快速检测及实验室Mhp和Mhr的鉴定,可为Mhp和Mhr在猪群中的早期监测提供技术支持。 展开更多
关键词 猪肺炎支原体 猪鼻支原体 Taqman荧光定量PCR
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circMAN1A2调节miR-212-3p/DDIT4轴对胃癌细胞增殖、侵袭和迁移的影响
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作者 曹博威 贾腾飞 +2 位作者 宋钉町 赵昱清 张文斌 《胃肠病学和肝病学杂志》 CAS 2024年第7期868-874,共7页
目的探究circMAN1A2靶向miR-212-3p/DNA损伤诱导转录子4(NDA damage-inducible transcript 4,DDIT4)轴对胃癌细胞增殖、侵袭和迁移的影响。方法qRT-PCR法检测胃癌组织和细胞中circMAN1A2、miR-212-3p表达水平。荧光素酶检测circMAN1A2与... 目的探究circMAN1A2靶向miR-212-3p/DNA损伤诱导转录子4(NDA damage-inducible transcript 4,DDIT4)轴对胃癌细胞增殖、侵袭和迁移的影响。方法qRT-PCR法检测胃癌组织和细胞中circMAN1A2、miR-212-3p表达水平。荧光素酶检测circMAN1A2与miR-212-3p、DDIT4和miR-212-3p的靶向关系。在胃癌细胞SGC-7901、HGC-27中转染si-NC、si-circMAN1A2、si-circMAN1A2+anti-NC、si-circMAN1A2+anti-miR-212-3p、miR-NC、miR-212-3p mimics,将未转染的SGC-7901、HGC-27细胞设为对照。平板克隆实验检测细胞增殖;细胞划痕及Transwell实验分别检测细胞迁移、侵袭能力。Western blotting检测增殖、迁移及侵袭相关蛋白表达。结果在胃癌组织及细胞中,circMAN1A2高表达,miR-212-3p低表达;下调circMAN1A2可以靶向上调miR-212-3p表达,且下调DDIT4表达,进而抑制胃癌细胞增殖、侵袭和迁移能力。抑制miR-212-3p表达可部分逆转抑制circMAN1A2表达对胃癌细胞恶性增殖的抑制作用。结论在胃癌细胞中抑制circMAN1A2表达可抑制胃癌细胞增殖、迁移与侵袭,其与调节miR-212-3p/DDIT4信号轴有关。 展开更多
关键词 环状RNA man1A2 微小RNA-212-3p DNA损伤诱导转录子4 胃癌 增殖 侵袭 迁移
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Tragic Fate of Tess and Man-made Morality ---Reflection of Tess of the d' Urbervilles
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作者 Wang Diqiu Hu Shanshan 《International English Education Research》 2015年第2期58-60,共3页
Tess of the d' Urbervilles, the greatest novel of British famous writer Thomas Hardy, describes the misfortune and tragedy of a poor peasant girl named Tess, the cause of which has always been the concern of people. ... Tess of the d' Urbervilles, the greatest novel of British famous writer Thomas Hardy, describes the misfortune and tragedy of a poor peasant girl named Tess, the cause of which has always been the concern of people. This passage will inquire into the cause of her tragedy from a critical point of view of feminism. In this passage, we can know the Tess takes shape from a social developing procession, man-made morality and the male writer. Then this article concludes that the consciousness of the females are given by the society in which they lived and the liberation of the females must be based on the awakening of self-consciousness and the change of moral sense of mortality in society. 展开更多
关键词 TESS TRAGEDY moralITY SELF-CONSCIOUSNESS
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猪细小病毒TaqMan荧光定量PCR检测方法的建立及应用
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作者 杨奕 吴发兴 +4 位作者 康京丽 孙洪涛 王志亮 许信刚 张琪 《动物医学进展》 北大核心 2024年第2期75-79,共5页
旨在建立一种猪细小病毒(PPV)快速准确PCR检测方法,根据PPV VP2基因的保守序列设计并合成1对特异性引物和1条特异性探针,建立了可检测PPV的TaqMan荧光定量PCR检测方法。建立的检测方法敏感性高,最低可检测8.76×10^(1)copies/μL的P... 旨在建立一种猪细小病毒(PPV)快速准确PCR检测方法,根据PPV VP2基因的保守序列设计并合成1对特异性引物和1条特异性探针,建立了可检测PPV的TaqMan荧光定量PCR检测方法。建立的检测方法敏感性高,最低可检测8.76×10^(1)copies/μL的PPV VP2基因标准品;特异性试验结果显示,该方法检测猪流行性腹泻病毒、猪圆环病毒2型、伪狂犬病病毒、猪繁殖与呼吸综合征病毒均无交叉反应;重复性试验组内与组间变异系数均低于2%,重复性良好。用该方法检测102份临床样本,检出阳性样本16份,阳性检出率为15.69%,将检测出的阳性样本进行PCR扩增并测序,证实检测样品中确实存在PPV。该方法敏感性高、特异性强,适用于PPV的定量检测及猪细小病毒病的流行病学调查。 展开更多
关键词 猪细小病毒 VP2 TAQman探针 荧光定量PCR
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Swimming Against Moral Currents:Gasping for Survival in Manjula Padmanabhan’s Harvest
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作者 Eugene Ngezem 《Journal of Philosophy Study》 2021年第8期631-636,共6页
Manjula Padmanabhan’s Harvest(1997)depicts despoiled and despondent characters whose change of fortunes compel them to careen between two conflicting cultures and squash their revered moral etiquettes as their vulner... Manjula Padmanabhan’s Harvest(1997)depicts despoiled and despondent characters whose change of fortunes compel them to careen between two conflicting cultures and squash their revered moral etiquettes as their vulnerable bodies are prey on and their lifestyles are distorted with impunity.Although the audience/readers may wriggle on Padmanabhan’s Harvest,circumstances drive her characters to swim against moral currents to survive in a world hanging on a string of economic collapse.Conscious of the dent their choices would put on their morality,these characters still surrender their rights to their own bodies and their pristine values to Ginni,a metaphor for western greed and exploitation.Ginni,via InterPlanta Services,tiptoes into the lives of impoverished Indian characters with promises of alleviating their poverty-stricken conditions,but quickly becomes a dour,barking orders to them with sternness,and leaving them battered and empty.Bruised by active resistance to western encroachment into their lives,Jaya defies the patriarchal system,even as her family members egg on her husband to give away everything for basic western needs. 展开更多
关键词 moralS values culture poverty exploitation collapse resistance DIGNITY and hope
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The classroom organizational management in the practice teaching of “Ideological and Moral Cultivation” in universities
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作者 黄晓晔 《海外英语》 2013年第11X期10-12,19,共4页
This article begins with the practice teaching of the course"Ideological and Moral Cultivation", to explore how to build a classroom organizational management mode, to highlight the applicability and practic... This article begins with the practice teaching of the course"Ideological and Moral Cultivation", to explore how to build a classroom organizational management mode, to highlight the applicability and practicality of teaching, striving to make this course teaching matches its nature of practice, to enhance the effectiveness of teaching. 展开更多
关键词 " Ideological and moral Cultivation" PRACTICE TEAC
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Management of College Students under the Ideaof Morality Education
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作者 Zhu Yan 《教育研究前沿(中英文版)》 2019年第3期159-163,共5页
Morality education is not only the foundation of education, but also the foundation of life, colleges and universities should keeppace with the times, innovate the concept of education, and integrate the concept of mo... Morality education is not only the foundation of education, but also the foundation of life, colleges and universities should keeppace with the times, innovate the concept of education, and integrate the concept of morality education into the management andideological and political education of colleges and universities. Based on this, in order to provide data support for this study, aquestionnaire survey was adopted to sample the teachers and students of Sichuan University to understand the current situation ofthe concept of "morality education" in colleges and universities, and the importance of the concept of "morality education" in themanagement of college students and the difficulties it faces were expounded, then pertinent suggestions were put forward for thecurrent problems. Implementing the concept of "morality education" in the management of colleges and universities caneffectively improve the quality of students, and guide students to establish correct political beliefs and promote their all-rounddevelopment. 展开更多
关键词 morality Education Education management Education Reform Quality Education
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The Death of Woman Wang—Morality in Crack
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作者 Manwei Zhang 《教育研究前沿(中英文版)》 2019年第4期360-362,共3页
The Death of Woman Wang tells a story about a married woman who eloped with her lover and finally was murdered by her husband.Nevertheless,the impressive and amazing part of this book is the perspective that the autho... The Death of Woman Wang tells a story about a married woman who eloped with her lover and finally was murdered by her husband.Nevertheless,the impressive and amazing part of this book is the perspective that the author has taken,rather than the story itself,an view that standing from the complete era,including backgrounds of politics,history and economy,making the plots in this book reasonable through the writing technique which has no subjective position.Morality has played a part of vital importance with its irreplaceability in this work,so the following article will start from ethic,then describe and analyse the use and role it demonstrates in this book. 展开更多
关键词 The Death of Woman Wang moralITY HISTORY
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虾十足目虹彩病毒1、肝肠胞虫及传染性早熟病毒多重Taq Man荧光定量PCR检测方法的建立与应用
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作者 徐晔 周毅 +4 位作者 王娜 窦维伟 王静 王凤芝 段宏安 《中国预防兽医学报》 CAS CSCD 北大核心 2024年第8期825-831,共7页
为建立检测十足目虹彩病毒1(DIV1)、肝肠胞虫(EHP)、传染性早熟病毒(IPV)的多重Taq Man荧光定量PCR检测方法,本研究根据DIV1 MCP基因、EHP SSU基因与IPV polyprotein A基因设计特异性引物和Taq Man探针,经各反应条件的优化建立了同时检... 为建立检测十足目虹彩病毒1(DIV1)、肝肠胞虫(EHP)、传染性早熟病毒(IPV)的多重Taq Man荧光定量PCR检测方法,本研究根据DIV1 MCP基因、EHP SSU基因与IPV polyprotein A基因设计特异性引物和Taq Man探针,经各反应条件的优化建立了同时检测DIV1、EHP及IPV的多重Taq Man荧光定量PCR方法。利用该方法检测DIV1、EHP、IPV、白斑综合征病毒、致急性肝胰腺坏死弧菌、传染性皮下及造血器官坏死病毒、虾偷死病毒、传染性肌肉坏死病毒、桃拉病毒、黄头病毒1型等主要虾类病毒,结果显示,该方法仅能扩增出DIV1、EHP和IPV,与其他主要虾类病原均无交叉反应,特异性较强。对10倍倍比稀释后等浓度等体积混合的DIV1、EHP和IPV质粒标准品混合物的检测结果显示,该方法对3种病原的质粒标准品的检测限均为1.67×10^(1)拷贝/μL,各病毒单一荧光定量PCR方法的检测限均为1.67×10~0拷贝/μL,多重Taq Man荧光定量PCR方法的灵敏度虽略低于单一荧光定量PCR方法,但是可以同时检测3种病原,检测效率高。选取3个浓度的pDIV1、pEHP、pIPV(5×10^(5)拷贝/μL、5×10^(4)拷贝/μL、5×10^(3)拷贝/μL)等浓度等体积混合的质粒标准品为模板,进行组内和组间重复性试验,结果显示组内与组间重复性试验的变异系数均小于1%,该方法重复性较好。利用该方法与DIV1、EHP、IPV单一荧光定量PCR方法同时检测105份虾苗和亲虾样品,结果显示,本研究建立的多重Taq Man荧光定量PCR方法对DIV1的检出率为21.9%(23/105),对EHP的检出率为14.3%(15/105),对IPV的检出率为45.7%(48/105),3种病原混合感染率为1.9%(2/105),与DIV1、EHP、IPV各单一荧光定量PCR方法的检测结果均一致,阳性样品的符合率达100%。本研究建立的DIV1、EHP、IPV多重Taq Man荧光定量PCR方法为临床同时检测该3种病原提供了特异、敏感、高效的技术手段。 展开更多
关键词 虾十足目虹彩病毒1 虾肝肠胞虫 虾传染性早熟病毒 多重Taq man荧光定量PCR
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Discussion on the Modern Moral Education Function of the Traditional "Five Human Relationships"
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作者 Guo Yuan 《International Journal of Technology Management》 2013年第7期67-69,共3页
Chinese culture puts much emphasis upon ethics, by which people are bound to pay respect to their superiors, fathers or husbands, hence a realization of social harmony and the utmost of the moral unification of heaven... Chinese culture puts much emphasis upon ethics, by which people are bound to pay respect to their superiors, fathers or husbands, hence a realization of social harmony and the utmost of the moral unification of heavens and human beings. The story as a means of significance in transmission of traditional culture explains vividly the connotation of traditional morals and plays a role of importance in moral education of ancient times as well. This thesis shall analyze the essence of moral ideas in those stories, through which to be aware of and taste the ethical tenderness in traditional moralities, thus a perfection of the application of traditional stories to modern moral education. 展开更多
关键词 Traditional stories Ideas of the five ethics moral education
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