Co-hosted by China Society for Hydropower Engineering and China Three Gorges Project Corporation, co-organized by China Hydropower Engineering Consulting Group Corporation , SINOHYDRO Corporation, China Gezhouba (Grou...Co-hosted by China Society for Hydropower Engineering and China Three Gorges Project Corporation, co-organized by China Hydropower Engineering Consulting Group Corporation , SINOHYDRO Corporation, China Gezhouba (Group) Corporation and China Institute of Water Resources and Hydropower Research, organized by China Electric Power Information Center Beijing Zhongdianqiuzhen T&DCo., Ltd., supported by State Electricity Regulatory Commission of China, State Grid Corporation of China, China Southern Power Grid Co., Ltd., China展开更多
在高等数学中关于极限的讨论,一般是既普遍又复杂的一个问题。我们在此就sum from i=1 to n a<sub>1</sub>b<sub>n-i+1</sub>形式的极限进行讨论。定理1（Mertens）设sum from a<sub>n</sub> t 和s...在高等数学中关于极限的讨论,一般是既普遍又复杂的一个问题。我们在此就sum from i=1 to n a<sub>1</sub>b<sub>n-i+1</sub>形式的极限进行讨论。定理1（Mertens）设sum from a<sub>n</sub> t 和sum from b<sub>n</sub> 两无穷级数收敛且至少有一个绝对收敛,又C<sub>n</sub>=a<sub>1</sub>b<sub>n</sub>+a<sub>2</sub>b<sub>n-1</sub>+…展开更多
AIM:To investigate the events associated with the apoptotic effect of p-Coumaric acid,one of the phenolic components of honey,in human colorectal carcinoma(HCT-15)cells.METHODS:3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny...AIM:To investigate the events associated with the apoptotic effect of p-Coumaric acid,one of the phenolic components of honey,in human colorectal carcinoma(HCT-15)cells.METHODS:3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltertazolium-bromide assay was performed to determine the antiproliferative effect of p-Coumaric acid against colon cancer cells.Colony forming assay was conducted to quantify the colony inhibition in HCT15 and HT 29 colon cancer cells after p-Coumaric acid treatment.Propidium Iodide staining of the HCT15 cells using flow cytometry was done to study the changes in the cell cycle of treated cells.Identification of apoptosis was done using scanning electron microscope and photomicrograph evaluation of HCT 15cells after exposing to p-Coumaric acid.Levels of reactive oxygen species(ROS)of HCT 15 cells exposed to p-Coumaric acid was evaluated using 2’,7’-dichlorfluorescein-diacetate.Mitochondrial membrane potential of HCT-15 was assessed using rhodamine-123 with the help of flow cytometry.Lipid layer breaks associated with p-Coumaric acid treatment was quantified using the dye merocyanine 540.Apoptosis was confirmed and quantified using flow cytometric analysis of HCT15 cells subjected to p-Coumaric acid treatment after staining with YO-PRO-1.RESULTS:Antiproliferative test showed p-Coumaric acid has an inhibitory effect on HCT 15 and HT 29 cells with an IC50(concentration for 50%inhibition)value of 1400 and 1600μmol/L respectively.Colony forming assay revealed the time-dependent inhibition of HCT 15 and HT 29 cells subjected to p-Coumaric acid treatment.Propidium iodide staining of treated HCT 15cells showed increasing accumulation of apoptotic cells(37.45±1.98 vs 1.07±1.01)at sub-G1phase of the cell cycle after p-Coumaric acid treatment.HCT-15 cells observed with photomicrograph and scanning electron microscope showed the signs of apoptosis like blebbing and shrinkage after p-Coumaric acid exposure.Evaluation of the lipid layer showed increasing lipid layer breaks was associated with the growth inhibition of p-Coumaric acid.A fall in mitochondrial membrane potential and increasing ROS generation was observed in the p-Coumaric acid treated cells.Further apoptosis evaluated by YO-PRO-1 staining also showed the timedependent increase of apoptotic cells after treatment.CONCLUSION:These results depicted that p-Coumaric acid inhibited the growth of colon cancer cells by inducing apoptosis through ROS-mitochondrial pathway.展开更多
文摘Co-hosted by China Society for Hydropower Engineering and China Three Gorges Project Corporation, co-organized by China Hydropower Engineering Consulting Group Corporation , SINOHYDRO Corporation, China Gezhouba (Group) Corporation and China Institute of Water Resources and Hydropower Research, organized by China Electric Power Information Center Beijing Zhongdianqiuzhen T&DCo., Ltd., supported by State Electricity Regulatory Commission of China, State Grid Corporation of China, China Southern Power Grid Co., Ltd., China
文摘在高等数学中关于极限的讨论,一般是既普遍又复杂的一个问题。我们在此就sum from i=1 to n a<sub>1</sub>b<sub>n-i+1</sub>形式的极限进行讨论。定理1（Mertens）设sum from a<sub>n</sub> t 和sum from b<sub>n</sub> 两无穷级数收敛且至少有一个绝对收敛,又C<sub>n</sub>=a<sub>1</sub>b<sub>n</sub>+a<sub>2</sub>b<sub>n-1</sub>+…
基金Supported by Universiti Teknologi Malaysia,Malaysia for providing Visiting Research Fellowship
文摘AIM:To investigate the events associated with the apoptotic effect of p-Coumaric acid,one of the phenolic components of honey,in human colorectal carcinoma(HCT-15)cells.METHODS:3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltertazolium-bromide assay was performed to determine the antiproliferative effect of p-Coumaric acid against colon cancer cells.Colony forming assay was conducted to quantify the colony inhibition in HCT15 and HT 29 colon cancer cells after p-Coumaric acid treatment.Propidium Iodide staining of the HCT15 cells using flow cytometry was done to study the changes in the cell cycle of treated cells.Identification of apoptosis was done using scanning electron microscope and photomicrograph evaluation of HCT 15cells after exposing to p-Coumaric acid.Levels of reactive oxygen species(ROS)of HCT 15 cells exposed to p-Coumaric acid was evaluated using 2’,7’-dichlorfluorescein-diacetate.Mitochondrial membrane potential of HCT-15 was assessed using rhodamine-123 with the help of flow cytometry.Lipid layer breaks associated with p-Coumaric acid treatment was quantified using the dye merocyanine 540.Apoptosis was confirmed and quantified using flow cytometric analysis of HCT15 cells subjected to p-Coumaric acid treatment after staining with YO-PRO-1.RESULTS:Antiproliferative test showed p-Coumaric acid has an inhibitory effect on HCT 15 and HT 29 cells with an IC50(concentration for 50%inhibition)value of 1400 and 1600μmol/L respectively.Colony forming assay revealed the time-dependent inhibition of HCT 15 and HT 29 cells subjected to p-Coumaric acid treatment.Propidium iodide staining of treated HCT 15cells showed increasing accumulation of apoptotic cells(37.45±1.98 vs 1.07±1.01)at sub-G1phase of the cell cycle after p-Coumaric acid treatment.HCT-15 cells observed with photomicrograph and scanning electron microscope showed the signs of apoptosis like blebbing and shrinkage after p-Coumaric acid exposure.Evaluation of the lipid layer showed increasing lipid layer breaks was associated with the growth inhibition of p-Coumaric acid.A fall in mitochondrial membrane potential and increasing ROS generation was observed in the p-Coumaric acid treated cells.Further apoptosis evaluated by YO-PRO-1 staining also showed the timedependent increase of apoptotic cells after treatment.CONCLUSION:These results depicted that p-Coumaric acid inhibited the growth of colon cancer cells by inducing apoptosis through ROS-mitochondrial pathway.
