Development and application of KASP marker for high throughput detection of the seedless trait in grapevine

Molecular marker-assisted selection(MAS) can significantly accelerate and improve the efficiency of the breeding process in seedless grape cultivars. In this study, we developed the KASP_VviAGL11 and VviAGL11_410 markers based on a single nucleotide polymorphism(SNP) site(Chr18: 26889437(A/C)) of the VviAGL11 gene, and compared them with previously reported SSR markers p3_VvAGL11 and 5U_VviAGL11 by testing 101 cultivars and 81 F1 hybrid progenies. The results showed that both of the proposed markers obtained 100% accuracy rates in detecting allele A, which was closely associated with the seedless trait in grapes, while p3_VvAGL11 and 5U_VviAGL11 had lower accuracy rates due to their tendency to produce false positives. After careful evaluation of the technical advantages and disadvantages associated with these markers, we concluded that KASP_VviAGL11 was superior in terms of simplicity,cost-effectiveness, efficiency, and accuracy. Thus, we optimized the process of molecular MAS for seedless grapes,focusing on the KASP_VviAGL11 marker as a central component, to provide key technical support for the development of new seedless grape cultivars.

Immature Embryo Rescue and in Vitro Development Evaluation of Intraspecific Hybrids from Brazilian Seedless Grapevine“Superior×Thompson”Clones

The fruit production for export is an economically significant activity in the Valley of S?o Francisco River, especially in the irrigated lands of Petrolina-PE/Juazeiro-BA, Brazil. The development of new genetic material most suitable to the tropical climate and the demands of the consumer market have led to the selection of new seedless grapes cultivars. In this case, the use of the embryo rescue technique has produced satisfactory results for obtaining such materials, especially in the semiarid region. This study aimed to evaluate the in vitro development of intraspecific hybrids of grapevine (Vitis vinifera L.), derived from the rescue of immature embryos resultant from the crossing of “Superior Seedless” and “Thompson Seedless” Brazilian clones. To establish and develop the cultivation, the culture media was supplemented with 30 g/L sucrose, 0.1 g/L myo-inositol, 0.002 g/L glycine, 0.1 mg/L indoleacetic acid (IAA), 6.5 g/L of agar, adjusted pH to 5.7. The experiment was evaluated after 90 days. The variables measured were: number of nodes, number of leaves, plant height (cm), number of roots and length (cm) of the root system and internodes. The period of 60 days of in vitro culture of ovules resulted in the highest values of embryos (about 50%), as well as better characterized developmental stages with higher germination (47.3%). The three types of hybrid grapes evaluated in micropropagation showed very similar values of the measured parameters, even having originated from embryos of different developmental stages.

Effects of Different Concentrations of Paclobutrazol on Seedling Quality of Small Seedless Watermelon in Autumn

[Objectives]The paper was to explore the effects of different concentrations of paclobutrazol on seedling quality of small seedless watermelon in autumn.[Methods]The leaves of watermelon seedlings were sprayed with 30,50,80 and 120 mg/L paclobutrazol at cotyledon expansion stage,and those sprayed with tap water were used as control.[Results]All the treatments of paclobutrazol effectively inhibited the excessive growth of watermelon at seedling stage,significantly enhanced the leaf quality of watermelon seedlings and promoted root growth.The seedlings treated by 50 mg/L paclobutrazol had the best performance compared with the control:a decrease of hypocotyl by 37.9%,an increase of plant dry weight by 46.55%,significantly increased seedling strength index,deep green leaf color,thick leaves,an increase of specific leaf weight by 28.13%,and significantly heavier root weight and longer length.[Conclusions]The results will provide a reference for strong seedling cultivation of small seedless watermelon.

Screening Test of Small Seedless Watermelon Varieties in Beijing Area

[Objectives]This study was conducted to select small seedless watermelon varieties with excellent quality and high yield suitable for planting in Beijing.[Methods]Under the conditions of facility cultivation in Beijing,a screening test was conducted on seven small seedless watermelon varieties for growth process,field traits,yield traits,fruit quality,and disease resistance.[Results]Varieties Mitong,Motong and Tianbao Xiaowuzi had a short growth period and relatively stable growth,and bore fruit easily,and their female flowers were closely spaced.Their yields exceeded 60000 kg/hm~2,ranking in the top three among all varieties.The fruit had a thin skin,a bright red flesh color,and a high mass fraction of soluble solids in the center.The flesh was tight or crispy,and tasted good.They also showed good disease resistance.Overall,they showed excellent comprehensive agronomic traits,and are thus suitable for facility cultivation in Beijing and its surrounding areas.[Conclusions]This study provides a theoretical basis for the upgrading of varieties and the increase in production and farmers'income in the suburbs of Beijing.

葡萄无核晚熟新品种中葡萄18号的选育

中葡萄18号是以无核紫、玫瑰香杂交育种结合无核胚挽救技术选育出的无核晚熟新品种。该品种自然坐果平均单穗质量600 g,平均单粒质量7.3 g,果粒长椭圆形,紫色或紫红色,可溶性固形物含量(w,后同)18.0%,总糖含量16.2%,总酸含量3.06 g·kg^(-1),糖酸比达到54∶1,单宁含量686 mg·kg^(-1),风味甜香,品质上等。在河南郑州露地栽培成熟期为9月初。该品种树势中庸,适宜避雨栽培。

大果少核椪柑新品种华柑3号的选育

华柑3号是通过鄂柑1号椪柑珠心胚实生苗倍性变异而培育的四倍体大果少核椪柑新品种。果实扁圆形,果实大,平均横径74.08 mm,纵径62.63 mm,平均单果质量179.94 g,平均果皮厚度3.45 mm。果肉橙黄色,肉质脆嫩,酸甜爽口,风味浓,可溶性固形物含量(w,后同)为14.49%,可滴定酸含量为1.16%,可食率为71.6%,化渣性好;种子少,平均2.7粒·果^(-1);树势中等偏强,树姿较直立,结果母枝以春梢和秋梢为主;在湖北武汉地区,12月中下旬果实成熟,可挂树至翌年1月;丰产性中等,按3.0 m×4.0 m密度定植的5年生树,每666.7 m^(2)产量可达1500 kg;适宜在年均温度16℃左右,冬季无霜冻、绝对最低温度在-5℃以上的区域种植。

浙江地方特色品种温岭高橙无核潜力新种质创制及分子鉴定

【目的】温岭高橙是浙江台州地方特色品种,栽培历史悠久,且具有一定的保健和药用价值,但果实有核限制了其在台州以外地区的发展,因此利用细胞工程高效育种技术对温岭高橙进行无核化改良。【方法】通过2条育种途径,一是以二倍体温岭高橙为母本与异源四倍体为父本倍性杂交,授粉后90 d利用幼胚离体挽救培养和流式细胞仪倍性鉴定创制三倍体无核新种质;二是以雄性不育胞质杂种华柚2号为母本与温岭高橙有性杂交,通过成熟种子催芽播种,创制二倍体无核新种质。利用SSR分子标记对杂交后代进行分子鉴定。【结果】以温岭高橙为母本、异源四倍体NS和BDZNS为父本配置2个倍性杂交组合,授粉145朵花,坐果74个;胚抢救幼嫩种子1520粒,离体培养获得再生植株553株;用流式细胞仪对再生植株进行倍性鉴定,获得三倍体45株、四倍体8株;以华柚2号为母本与温岭高橙有性杂交,授粉69朵花,坐果20个,获得成熟种子312粒;通过催芽播种获得实生后代203株,倍性鉴定均为二倍体;用SSR分子标记对2个倍性杂交组合的21株三倍体和8株四倍体后代及华柚2号×温岭高橙随机选取的29株二倍体后代进行分子鉴定,显示所鉴定的三倍体及二倍体后代均含有父本特异条带,为其杂交双亲的有性后代,而倍性杂交获得的四倍体后代与母本条带完全一致,推测其为母本珠心细胞自然加倍形成的同源四倍体(双二倍体)。【结论】研究结果为温岭高橙无核新品种培育奠定了宝贵的材料基础。

鲜食葡萄无核化研究进展

优质、大粒、无核鲜食葡萄深受消费者青睐,利用植物生长调节剂进行葡萄无核化生产已成为获取优质、大粒、无核鲜食葡萄的重要途径,并在生产上得到广泛应用。本文综述了葡萄无核化机理、常用植物生长调节剂种类及其使用浓度和处理方法、无核化栽培技术及需注意的问题等,以期为葡萄无核化生产提供理论依据和参考。

无籽西瓜新品种墨绿无籽5号的选育

墨绿无籽5号是以自交系GR4x-2为母本,以2x-55为父本配制而成的大果型无籽西瓜一代杂种。中晚熟,早春大棚栽培全生育期112 d(天)左右,果实发育期40 d(天)左右。果实正圆形,果皮墨绿色覆深绿色网条纹,果皮厚度1.2 cm,硬度27 kg·cm^(-2)左右,耐裂、耐贮运;瓜瓤红色,中心可溶性固形物含量约12.1%,边部可溶性固形物含量约9.8%。

延迟环剥处理对克瑞森葡萄果实品质的影响

以优质鲜食葡萄品种克瑞森无核为试材,设置一次环剥和延迟环剥2个处理,以常规管理为对照。于7月28日至9月17日每隔10 d采样1次,共采集6次,对单粒重、粒径、果形指数、色差等外在指标,可溶性固形物、可滴定酸、V_(C)、糖酸组分含量等内在指标进行测定,以期为新疆产区克瑞森无核葡萄促早提质栽培技术的应用提供理论依据。结果表明,延迟环剥处理对克瑞森葡萄果形指数无显著影响,一次环剥处理可以显著提高单粒重,延迟环剥处理对单粒重无显著影响;一次环剥和延迟环剥处理均显著促进果实中可溶性固形物的积累,显著提高固酸比,促进果实成熟;糖酸组成及含量比例有很大的不同,其中,葡萄糖含量、果糖含量最高的都是一次环剥处理。一次环剥处理明显提高了糖含量。延迟环剥对酒石酸、苹果酸与柠檬酸含量有显著加速下降的趋势,一次环剥草酸含量有所降低。克瑞森葡萄一次环剥和延迟环剥处理V_(C)含量显著提高,表明延迟环剥对克瑞森葡萄V_(C)含量增加有明显作用。克瑞森葡萄不同时期环剥处理中,延迟环剥处理能明显改善克瑞森的着色,提高亮度,促进果实着红色。

无籽西瓜新品种晋花无籽4号的选育

晋花无籽4号是以四倍体自交系GR4X-2为母本、二倍体自交系2X-59为父本杂交育成的中果型无籽西瓜新品种。该品种植株生长势强,中晚熟,在山西省晋中地区早春大棚栽培果实发育期40 d,中抗枯萎病。主蔓、侧蔓均可连续坐果,果实圆形,果面绿底覆墨绿色齿条纹,瓜瓤粉红色,着色秕籽少,无籽性好。中心和边部可溶性固形物含量(w,后同)分别为12.7%、10.5%,果皮硬度大,皮韧耐贮运。平均单果质量5.4 kg,667 m^(2)产量4400~4500 kg,果实商品率90%,适宜在山西地区春提早设施栽培。2023年3月通过农业农村部非主要农作物品种登记。

葡萄褐纹病菌侵染过程的细胞学特性研究

为明确褐纹病菌侵染葡萄叶片的过程,采用光学显微镜、扫描电镜和透射电镜等技术手段,系统研究了葡萄褐纹病菌在‘无核白’和‘克瑞森无核’葡萄叶片上的侵染过程。结果表明:葡萄褐纹菌分生孢子在‘无核白’‘克瑞森无核’葡萄叶片接种1 d后均可萌发;后期发育过程,‘克瑞森无核’葡萄叶片上菌丝细长,生长停滞;‘无核白’葡萄叶片表面初生菌丝密布,持续在寄主体表生长扩展;接种3 d,‘无核白’葡萄叶片的表面形成了密集的菌丝网,同时观察到菌丝从气孔或直接入侵方式进入叶片表皮组织内部,随后,菌丝以胞间和胞内生长的方式扩展;接种6 d,菌丝达到上表皮,随菌丝在叶片组织内不断地生长扩展,使得寄主细胞坏死、解体。可见:‘无核白’是亲和葡萄褐纹病菌品种,而‘克瑞森无核’葡萄非亲和褐纹病菌。

4种植物生长调节剂对无核白葡萄果刷生长发育及采后落粒性的影响

无核白葡萄是我国重要的鲜食和制干兼用品种,但生产中普遍存在采后落粒问题。设置采前4种植物生长调节剂处理(100 mg/L S-ABA、10μmol/L MeJA、100 mg/L NAA+100 mg/L 6-BA、25 mg/L 2,4-D)研究无核白落粒率与果梗拉力的变化,以及果刷和其他相关指标间的联系。结果表明:2,4-D处理落粒率降低、果梗拉力增大,对应的果刷纵切与横切情况下的木质素染色面积显著大于其他处理,与落粒率呈显著负相关关系,与果梗拉力呈显著正相关关系,而纤维素并没有这种相关性;2,4-D处理上部果皮厚度显著大于其他处理,与落粒率呈负相关关系,与果梗拉力、果刷长度、果刷纵切及横切木质素染色面积呈显著正相关,但中部果皮厚度与其他指标不存在相似的关系;主成分分析结果显示,综合9个指标评价4种处理提高果梗耐拉力的效果由强到弱为25 mg/L 2,4-D>10μmol/L MeJA>100 mg/L NAA+100 mg/L 6-BA>100mg/L S-ABA。综上所述,无核白果实的果刷长度和其木质素含量是影响落粒率的重要因素,2,4-D可以诱导果刷内木质素积累,为果实落粒防控提供科学依据。

Glan-Thompson棱镜高性能宽带减反射膜的研制及性能测试

Glan-Thompson棱镜在光学领域有着重要的应用,透过比的高低和有效带宽的大小,直接影响着棱镜的品质.为了提高棱镜的透过比,拓宽有效使用带宽,借助于计算机辅助设计方法,设计了高性能多层减反射膜系;选用合适的光学薄膜材料,利用电子束蒸镀,借助于离子源辅助蒸镀,制作了高性能的宽带减反射膜.测试结果表明:平均剩余反射率小于0.5%,有效使用带宽在可见光区大于100 nm,在近红外大于200nm,达到了设计要求,提升了棱镜的品质.

葡萄无核性状分子标记通用性验证

【目的】利用葡萄自然群体对已有9个无核分子标记进行评价,验证其无核检测通用性效果,为加快无核葡萄新品种选育进程提供技术支持。【方法】以88个无核种质和120个有核种质组成的自然群体为试材,对国内外已发表的9个常用葡萄无核分子标记进行通用性验证。【结果】SCAR类型标记GSLP1-569、SCC8-1080和SCF27-2000的鉴定准确率分别为57.12%、72.20%和75.38%,无核检测率分别为90.51%、79.18%和67.82%。SSR类型标记p1-VvAGL11、p2-VvAGL11、p3-VvAGL11、5U_VviAGL11、VMC7F2和VVSD10的鉴定准确率分别是88.47%、67.43%、71.94%、68.47%、67.99%和61.60%,无核检测率分别是87.74%、77.22%、90.72%、90.80%、79.10%和63.03%;卡方分析表明与无核表型极显著相关的等位点分别是250 bp、171 bp、195 bp、315 bp、197 bp和105 bp。【结论】SCAR标记SCF27-2000和SSR标记p1-VvAGL11准确率高,综合表现最优,适用于无核葡萄新品种的分子辅助选择。

‘无核白’葡萄热风干燥特性及色泽研究

为探究干燥温度、装载密度和风速对‘无核白’葡萄干燥特性及色泽的影响,通过调查制干过程中葡萄重量变化,葡萄干含水量、色差,以及总酚、叶绿素、总糖、总酸含量等品质指标,计算出干率、绿品率、成干时间,分析不同处理对加工效率和葡萄干品质的影响。结果表明,干燥时间与物料温度和风速呈正相关,与装载量呈负相关,葡萄干总糖含量与果实的干燥工艺无相关性。当干燥温度为40℃时,‘无核白’葡萄会发生明显的褐变;而装载量超过15.62 kg·m^(-3)和风速超过2 m·s^(-1)时,褐变现象逐渐加重。综上,温度为35℃、风速1.0 m·s^(-1)、装载量7.81 kg·m^(-3)时,‘无核白’葡萄干燥时间为148 h,叶绿素、总酚含量分别为0.55、0.15 mg·g^(-1),与原材料色差为6.99,绿品率达到64%。葡萄干整体表现颗粒饱满,色泽良好。

前臂近中段Thompson入路中桡神经深支医源性损伤的解剖学因素及对策

目的探讨前臂近中段Thompson入路中容易发生桡神经深支损伤的解剖学因素并提出对策。方法教学用成人尸体标本48具,按照Thompson入路显露,观察前臂伸肌与桡神经深支及其肌支的位置关系;测量桡神经深支穿出旋后肌的位置距离桡侧腕短伸肌的横向距离;观察指伸肌和桡侧腕短伸肌在前臂近段的愈着情况,测量二者在肱桡关节线以远的愈着长度。结果指伸肌和桡侧腕短伸肌在前臂近段相愈着,愈着长度在肱桡关节以远(7.1±2.1)cm;桡神经深支在旋后肌下缘的穿出点与桡侧腕短伸肌尺侧缘的水平距离为(1.3±0.3)cm,与肱桡关节线距离为(6.1±1.8)cm。结论前臂近中段Thompson入路中容易发生桡神经深支损伤存在着解剖学因素,了解桡神经深支的解剖特点及其与前臂伸肌的位置关系可以避免桡神经深支损伤。

无核荔枝僵果病的病原菌鉴定、入侵途径及快速检测技术

2022年在海南无核荔枝上发现一种僵果病,与海南常见的荔枝病害炭疽病、酸腐病等病症差别明显,主要为害幼果,发病时幼果果实外观无明显变化,但无核荔枝完成疏果后,果实长至蚕豆大小时,发病果实的外果皮颜色变暗,剖开后内果皮发生不规则褐变,果实不再膨大,形成僵果,易造成严重的经济损失。本研究对该病害的病原菌进行分离和鉴定,对入侵途径和快速检测技术进行研究。结果表明:通过形态学鉴定、ITS、RBP2和TEF1基因序列分析,鉴定引起无核荔枝新型僵果病的病原菌为伯氏镰刀菌(Fusarium pernambucanum)。无核荔枝幼果期果实果皮受农事操作、风、刺吸性昆虫为害等损伤后,易受到病原菌的入侵发病,而果实表面无伤口时不发病。病原菌入侵主要集中在果实纵经3.2~4.8 mm的幼果期,随着果实的生长、果皮保护组织的增加和伤口的减少,病原菌难以入侵,果实发病率快速降低。基于TUB基因序列,以伯氏镰刀菌及其他分离获得的镰刀菌属菌株基因组DNA为模板进行扩增测序,根据序列信息设计筛选出一对伯氏镰刀菌的特异性引物,构建了基于普通PCR的快速高效分子检测方法,该检测方法灵敏、高效,可用于指导无核荔枝僵果病的早期诊断与预防。

Thompson变换－FDTD方法模拟不规则形体的电磁散射问题

三角形由于尖锐的角点，半环形由于既有凹面又有角点，它们都给传统的ＦＤＴＤ数值求解造成了一定的困难。这是因为要获得较高的精度，必须细分网格，从而增加内存需求和计算时间。同时三角形柱和半环柱对Ｔｈｏｍｐｓｏｎ－ＦＤＴＤ方法来说也是“坏”的形状，它使得Ｔｈｏｍｐｓｏｎ变换的精度有所下降。本文通过模拟这两种典型的不利形体的散射问题。

An Effective Method for Extracting Total RNA from Young Embryo of Seedless Litchi

A total RNA extraction method for young embryo of seedless litchi was introduced. CTAB, Phenol （saturated with water）, chloroform, Guanidine isothioeyanate were used as main extraction reagents. Polyphenolie compounds were removed effectively by added PVP into the extraction buffer solution. RNA was purified intensively by phenol, chloroform extraction, and ethanol deposition after deposited by LiCl. Both the results of formaldehyde denatured agarose gel eleetrophoresis and ultraviolet spectrophotometer analysis showed high integrity and purity of RNA. So the quality of extracted RNA could meet the demand of most molecular biology experiments that require higher quality RNA.