Abstract： To investigate the chemical constituents of the roots of Polygala wattersii Hance, the separation and purification were performed by solvent extraction and repeated column chromatography （CC） on silica ge...Abstract： To investigate the chemical constituents of the roots of Polygala wattersii Hance, the separation and purification were performed by solvent extraction and repeated column chromatography （CC） on silica gel, Sephadex LH-20 and macroporous resin D101, preparative TLC and semi-preparative HPLC. The structures were identified by spectroscopic analysis and comparison of their 1H and 13C NMR data with those reported in literatures. Twenty-three known compounds, including eleven xanthones （1-11）, nine sugar esters （12-20）, two triterpenoid saponins （21 and 22） and one phenylpropanoid （23） were isolated and their structures were identified as 1,3-dihydroxyxanthone （1）, 1-hydroxy-3-methoxyxanthone （2）, 1,3-dihydroxy-2-methoxyxanthone （3）, 1,3,7-trihydroxy-2- methoxyxanthone （4）, 1,3,6-trihydroxy-2,7-dimethoxyxanthone （5）, 1,6,7-trihydroxy-2,3-dimethoxyxanthone （6）, 1,7-dihydroxy- 2,3-methylenedioxyxanthone （7）, 1,7-dimethoxyxanthone （8）, 1,2,3-trimethoxyxanthone （9）, 1-methoxy-2,3-methylenedioxyxanthone （10）, 6-hydroxy-0-methoxy-2,3-methylenedioxyxanthone （11）, 3＇-O-feruloyl-6-O-acetyl sucrose （12）, arillatose B （13）, sibricose A5 （14）, sibricose A6 （15）, 3＇,6-di-O-sinapoyl sucrose （16）, tenufoliside A （17）, 3＇-O-3,4,5-trimethoxycinnamoyl-6-O-p-methoxybenzoyl sucrose （18）, glomeratose A （19）, 1-O-p-coumaroyl-D-glucopyranose （20）, bayogenin-3-O-glucoside （21）, tenufolin （22）, and sinapic acid （23）. Among them, compounds 2 and 12 were obtained from genus Polygala for the first time, and except compound 16, all others were isolated from this species for the first time.展开更多
Eight known compounds were isolated from the whole plants ofArenaria serpyllifolia L. by solvent fractionation and subsequent separations with silica gel, ODS, Sephadex LH-20 and semi-preparative HPLC chromatography. ...Eight known compounds were isolated from the whole plants ofArenaria serpyllifolia L. by solvent fractionation and subsequent separations with silica gel, ODS, Sephadex LH-20 and semi-preparative HPLC chromatography. Their structures were determined by spectroscopic analysis and identified as quercetin （1）, quercetin 7-O-α-L-rhamnoside （2）, quercetin 3-O-α-L- rhamnoside （3）, 3-O-methyl quercetin （4）, 1,5-dihydroxyxanthone （5）, 1,5-dihydroxyxanthone-6-O-β-D-glucoside （6）, 5-hydroxy- l-methoxyxanthone （7） and 6-deoxyisojacareubin （8）. All these compounds were isolated from this title plant for the first time, and compounds 5-8 were obtained from genus Arenaria for the first time.展开更多
基金The National Key Technology R&D Program"New Drug Innovation"of China(Grant No.2012ZX09301002-002-002,2012ZX09304-005)special funds for scientific research on traditional Chinese medicine(Grant No.201307002)National Science Fund for Excellent Young Scholars(Grant No.81222051)
文摘Abstract： To investigate the chemical constituents of the roots of Polygala wattersii Hance, the separation and purification were performed by solvent extraction and repeated column chromatography （CC） on silica gel, Sephadex LH-20 and macroporous resin D101, preparative TLC and semi-preparative HPLC. The structures were identified by spectroscopic analysis and comparison of their 1H and 13C NMR data with those reported in literatures. Twenty-three known compounds, including eleven xanthones （1-11）, nine sugar esters （12-20）, two triterpenoid saponins （21 and 22） and one phenylpropanoid （23） were isolated and their structures were identified as 1,3-dihydroxyxanthone （1）, 1-hydroxy-3-methoxyxanthone （2）, 1,3-dihydroxy-2-methoxyxanthone （3）, 1,3,7-trihydroxy-2- methoxyxanthone （4）, 1,3,6-trihydroxy-2,7-dimethoxyxanthone （5）, 1,6,7-trihydroxy-2,3-dimethoxyxanthone （6）, 1,7-dihydroxy- 2,3-methylenedioxyxanthone （7）, 1,7-dimethoxyxanthone （8）, 1,2,3-trimethoxyxanthone （9）, 1-methoxy-2,3-methylenedioxyxanthone （10）, 6-hydroxy-0-methoxy-2,3-methylenedioxyxanthone （11）, 3＇-O-feruloyl-6-O-acetyl sucrose （12）, arillatose B （13）, sibricose A5 （14）, sibricose A6 （15）, 3＇,6-di-O-sinapoyl sucrose （16）, tenufoliside A （17）, 3＇-O-3,4,5-trimethoxycinnamoyl-6-O-p-methoxybenzoyl sucrose （18）, glomeratose A （19）, 1-O-p-coumaroyl-D-glucopyranose （20）, bayogenin-3-O-glucoside （21）, tenufolin （22）, and sinapic acid （23）. Among them, compounds 2 and 12 were obtained from genus Polygala for the first time, and except compound 16, all others were isolated from this species for the first time.
基金National Key Technology R&D Program"New Drug Innovation"of China(Grant No.2012ZX09301002-002-002,2012ZX09304-005)
文摘Eight known compounds were isolated from the whole plants ofArenaria serpyllifolia L. by solvent fractionation and subsequent separations with silica gel, ODS, Sephadex LH-20 and semi-preparative HPLC chromatography. Their structures were determined by spectroscopic analysis and identified as quercetin （1）, quercetin 7-O-α-L-rhamnoside （2）, quercetin 3-O-α-L- rhamnoside （3）, 3-O-methyl quercetin （4）, 1,5-dihydroxyxanthone （5）, 1,5-dihydroxyxanthone-6-O-β-D-glucoside （6）, 5-hydroxy- l-methoxyxanthone （7） and 6-deoxyisojacareubin （8）. All these compounds were isolated from this title plant for the first time, and compounds 5-8 were obtained from genus Arenaria for the first time.
