For better understanding the chemical or biological information of ZNF191 （243-368）, we expressed the fusion protein of GST and ZNF191 （243-368）, and used it to obtain the binding DNA sequence of this zinc finger ...For better understanding the chemical or biological information of ZNF191 （243-368）, we expressed the fusion protein of GST and ZNF191 （243-368）, and used it to obtain the binding DNA sequence of this zinc finger protein. But in the process of expression and purification, we found this fusion protein slowly degradated. For resolving this problem, we simultaneously added charged amino acids L-Arg and L-Glu to the solution of fusion protein, and demonstrated that this method can dramatically increase the stability of this fusion protein. This method can make the fusion protein suitable for the continuous works, especially for situations where high protein concentration and long-term stability without precipitate and degradation of protein are required.展开更多
This paper investigates a simplified model for describing the gravitational fields of non- axisymmetric elongated asteroids. The connection between the simplified model and the target aster-oid is built by considering...This paper investigates a simplified model for describing the gravitational fields of non- axisymmetric elongated asteroids. The connection between the simplified model and the target aster-oid is built by considering the positions of equilibrium points. To improve the performance of po-sition matching for the equilibrium points associated with these non-axisymmetric asteroids, a non-axisymmetric triple-particle-linkage model is proposed based on two existing axisymmetric particle- linkage models. The unknown parameters of the simplified model are determined by minimizing the matching error using the nonlinear optimization method. The proposed simplified model is applied for three realistic elongated asteroids, 243 Ida, 433 Eros and （8567） 1996 HW1. The simulation re-sults verify that the current particle-linkage model has better matching accuracy than the two existing particle-linkage models. The comparison, between the simplified model and the polyhedral model, on the topological cases of the equilibrium points and the distribution of gravitational potential further validate the rationality and accuracy of the simplified model.展开更多
We report results of our optical photometric observations of ten gamma-ray loud blazers, namely: 0219+428 (3C66A), PKS 0420-014 (OA 129), S5 0716+714, 0754+100 (OI 090.4), 0827+243 (OJ248), 1652+398 (Mrk 501), 2200+42...We report results of our optical photometric observations of ten gamma-ray loud blazers, namely: 0219+428 (3C66A), PKS 0420-014 (OA 129), S5 0716+714, 0754+100 (OI 090.4), 0827+243 (OJ248), 1652+398 (Mrk 501), 2200+420 (BL Lacertae), 2230+114 (CTA 102), 2251+158 (3C 454.3) and 2344+514. The observations were carried out in September-October, 2000 using the 70 cm optical telescope at Abstumani Observatory, Georgia. We found intra-day variations in 0420-014, S5 0716+714, BL Lacertae and CTA 102. A variation of 0.3 magnitude over a time scale of about 3 hours was observed in the R passband in BL Lacertae on JD 2451827. We did not detect any variation in 3C 66A, Mrk 501, or 3C 454.3 during our observations. Nor did we detect any clear evidence of variation in 1ES 2344+514 during our two weeks' observing run of the TeV gamma-ray source.展开更多
Severe bleeding is a common problem and a main cause of death in a medical setting,including surgery,trauma,congenital and acquired abnormalities of hemostasis,fulminant hepatic failure,and gastrointestinal bleeding.I...Severe bleeding is a common problem and a main cause of death in a medical setting,including surgery,trauma,congenital and acquired abnormalities of hemostasis,fulminant hepatic failure,and gastrointestinal bleeding.It has been estimated that rxcessive blood loss may contribute to approximately 30% of trauma-related deaths.Bleeding in critical locations,especially intracerebral hemorrhage,pose also a major clinical challenge.Severe bleeding often needs blood transfusion.In many patients with abnormalities of hemostasis,bleeding can be effectively corrected by replacing the defective components of the hemostatic system,of which the most widely used are factor Ⅷ concentrations and recombinant factor Ⅷ products for hemophilia A treatment,prothrombin complex concentrates and recombinant factor Ⅸ for hemophilia B treatment,and fibrinogen or cryoprecipitate for afibrinogenemia.On the other hand,all the cases of excessive bleeding require pharmacologic strategies of hemostatic agents to minimize blood loss.展开更多
Two mutants of the zinc finger protein, ZNF191(243—368) I323W and R327W, are suc-cessfully obtained by site-directed mutagenesis. The fluorescence spectrum is used to study the interaction between these two mutants a...Two mutants of the zinc finger protein, ZNF191(243—368) I323W and R327W, are suc-cessfully obtained by site-directed mutagenesis. The fluorescence spectrum is used to study the interaction between these two mutants and the oligonucleotides. The influence of the mutation on the interaction has been studied using ethidium bromide (EB) as the fluorescence probe. The binding constants of the I323W-DNA and R327W-DNA have been calculated and the possible binding models have been discussed.展开更多
文摘For better understanding the chemical or biological information of ZNF191 （243-368）, we expressed the fusion protein of GST and ZNF191 （243-368）, and used it to obtain the binding DNA sequence of this zinc finger protein. But in the process of expression and purification, we found this fusion protein slowly degradated. For resolving this problem, we simultaneously added charged amino acids L-Arg and L-Glu to the solution of fusion protein, and demonstrated that this method can dramatically increase the stability of this fusion protein. This method can make the fusion protein suitable for the continuous works, especially for situations where high protein concentration and long-term stability without precipitate and degradation of protein are required.
基金supported by the National Natural Science Foundation of China (Grant No.11672126)the Innovation Funded Project of Shanghai Aerospace Science and Technology (Grant Nos.SAST2017032 and SAST2015036)the Scientific Research Foundation for New Staffs of Nanjing University of Aeronautics and Astronautics (Grant No.1011-YAH17071)
文摘This paper investigates a simplified model for describing the gravitational fields of non- axisymmetric elongated asteroids. The connection between the simplified model and the target aster-oid is built by considering the positions of equilibrium points. To improve the performance of po-sition matching for the equilibrium points associated with these non-axisymmetric asteroids, a non-axisymmetric triple-particle-linkage model is proposed based on two existing axisymmetric particle- linkage models. The unknown parameters of the simplified model are determined by minimizing the matching error using the nonlinear optimization method. The proposed simplified model is applied for three realistic elongated asteroids, 243 Ida, 433 Eros and （8567） 1996 HW1. The simulation re-sults verify that the current particle-linkage model has better matching accuracy than the two existing particle-linkage models. The comparison, between the simplified model and the polyhedral model, on the topological cases of the equilibrium points and the distribution of gravitational potential further validate the rationality and accuracy of the simplified model.
基金Supported by the National Natural Science Foundation of China
文摘We report results of our optical photometric observations of ten gamma-ray loud blazers, namely: 0219+428 (3C66A), PKS 0420-014 (OA 129), S5 0716+714, 0754+100 (OI 090.4), 0827+243 (OJ248), 1652+398 (Mrk 501), 2200+420 (BL Lacertae), 2230+114 (CTA 102), 2251+158 (3C 454.3) and 2344+514. The observations were carried out in September-October, 2000 using the 70 cm optical telescope at Abstumani Observatory, Georgia. We found intra-day variations in 0420-014, S5 0716+714, BL Lacertae and CTA 102. A variation of 0.3 magnitude over a time scale of about 3 hours was observed in the R passband in BL Lacertae on JD 2451827. We did not detect any variation in 3C 66A, Mrk 501, or 3C 454.3 during our observations. Nor did we detect any clear evidence of variation in 1ES 2344+514 during our two weeks' observing run of the TeV gamma-ray source.
基金supported by a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Severe bleeding is a common problem and a main cause of death in a medical setting,including surgery,trauma,congenital and acquired abnormalities of hemostasis,fulminant hepatic failure,and gastrointestinal bleeding.It has been estimated that rxcessive blood loss may contribute to approximately 30% of trauma-related deaths.Bleeding in critical locations,especially intracerebral hemorrhage,pose also a major clinical challenge.Severe bleeding often needs blood transfusion.In many patients with abnormalities of hemostasis,bleeding can be effectively corrected by replacing the defective components of the hemostatic system,of which the most widely used are factor Ⅷ concentrations and recombinant factor Ⅷ products for hemophilia A treatment,prothrombin complex concentrates and recombinant factor Ⅸ for hemophilia B treatment,and fibrinogen or cryoprecipitate for afibrinogenemia.On the other hand,all the cases of excessive bleeding require pharmacologic strategies of hemostatic agents to minimize blood loss.
基金This work was supported by the National Natural Science Foundation of China (Grant Nos. 30170228 and 39990600).
文摘Two mutants of the zinc finger protein, ZNF191(243—368) I323W and R327W, are suc-cessfully obtained by site-directed mutagenesis. The fluorescence spectrum is used to study the interaction between these two mutants and the oligonucleotides. The influence of the mutation on the interaction has been studied using ethidium bromide (EB) as the fluorescence probe. The binding constants of the I323W-DNA and R327W-DNA have been calculated and the possible binding models have been discussed.
