期刊文献+
共找到4篇文章
< 1 >
每页显示 20 50 100
双重实时荧光PCR法检测食品和饲料中的鸡源性成分 被引量:13
1
作者 汪永信 安虹 +3 位作者 程坚 程潇 刘娟娟 张波 《生物技术通报》 CAS CSCD 北大核心 2012年第1期134-138,共5页
根据鸡线粒体DNA细胞色素b基因序列和内参照基因PUC18质粒基因序列设计特异性引物和以不同荧光素标记的TaqMan探针。通过对反应条件的优化筛选,建立能同时扩增鸡源性成分和内参照基因成分的双重实时荧光PCR检测方法。设置内参照反应是... 根据鸡线粒体DNA细胞色素b基因序列和内参照基因PUC18质粒基因序列设计特异性引物和以不同荧光素标记的TaqMan探针。通过对反应条件的优化筛选,建立能同时扩增鸡源性成分和内参照基因成分的双重实时荧光PCR检测方法。设置内参照反应是为了监控反应体系中是否含有PCR反应的抑制物,避免出现假阴性结果。分别以鸡、鸭、鹅、火鸡、牛、羊、猪、鱼、兔、驴、鹿、狗、马、鸽子、大豆、玉米、小麦、大米、马铃薯及番茄的线粒体DNA作为模板进行特异性试验,结果表明该方法仅能特异性扩增鸡源性成分,而对其它物种未见有效扩增。通过灵敏度测试,该方法检出限达0.01%。所制定的方法特异性高,灵敏度好,可以作为食品和饲料中鸡源性成分的高效检测方法。 展开更多
关键词 双重实时荧光PCR 细胞色素B基因 鸡源性成分 内参照 检测方法
下载PDF
印刷电路组件吸湿性尘粒人工模拟试验中关键因素的控制
2
作者 刘勇志 詹炀 +1 位作者 钟浩 晋晓峰 《环境技术》 2016年第5期19-22,共4页
吸湿性尘粒人工模拟试验(简称湿尘试验)是一种新型的环境试验。通过对试验方法和试验过程中各环节的研究,确定了试验时应把握的关键因素,并最终总结了试验中关键因素控制的方法。这些方法可为湿尘试验的开展提供借鉴作用。
关键词 印刷电路组件 湿尘试验 吸湿性尘粒 湿度阀值 人工模拟试验 关键因素 IPC-B-25试片 表面绝缘电阻
下载PDF
基于80C196单片机+PSD芯片的新型电除尘控制器
3
作者 张伟平 《山西科技》 2005年第5期104-105,107,共3页
文章介绍了新型电除尘控制器高度集成化的一种硬件设计方案,对其中几个关键组成单元进行了较为详细的说明,提出基于CAN总线的“对等式网络”设计构架,阐述了由本控制器组成的IPC系统采用B/S模式进行信息交互、资源共享的网络设计思想。
关键词 单片机 PSD芯片 CAN总线 IPC系统 B/S模式
下载PDF
Effect of kinetic needling combined with blood-letting puncturing and cupping on functions of upper limbs of patients with shoulder-hand syndrome after apoplexy 被引量:3
4
作者 刘未艾 吴清明 +2 位作者 付磊 李向荣 李丹丹 《World Journal of Acupuncture-Moxibustion》 2010年第1期7-12,23,共7页
Objective To observe influence of kinetic needling on functional restoration of upper limbs of patients with apoplexy-induced shoulder-hand syndrome. Methods Ninety patients were randomly divided into an observation g... Objective To observe influence of kinetic needling on functional restoration of upper limbs of patients with apoplexy-induced shoulder-hand syndrome. Methods Ninety patients were randomly divided into an observation group (45 cases) and a control group (45 cases). Basic treatments selected according to corresponding stroke units were applied to both groups. Besides, scalp acupuncture at Dingnie Qianxiexian (MS 6, 顶颞前斜线), Dingnie Houxiexian (MS 6, 顶颞前斜线) and Dingzhongxian (NS 5, 顶中线), was applied in the treatment group. In the observation group, active and passive movement was carried out during the process of scalp acupuncture, and then needling plus bloodletting puncturing and cupping was applied. In the control group, only needling plus blood-letting puncturing and cupping was given. Therapeutic effects, pain scoring obtained by visual analogue scales (VAS), FugI-Meyer and FugI-Meyer's scoring reflecting moving functions of upper limbs were made after 4 courses of treatments in both groups. Results The total effective rate was 95.5% and 91.1% in the observation group and control group, respectively, showing superiority of the former to the latter (P〈0.05). After treatment, VAS scores were all obviously reduced in both groups (P〈0.01), and the reduction in the former was significantly more than that in the latter (P〈0.01). Moving functions of upper limbs shown by Fugl-Meyer's scoring were improved after 2 courses of treatment in both groups (P〈0.01). The improvement was more obvious after 4 courses of treatment than that after 2 courses of treatment in both groups (P〈0.05). After 4 courses of treatment, moving functions shown by FugI-Meyer's scoring were more remarkably improved in the observation group than those in the control group(t=3.9, P〈0.01). Conclusion Both kinetic needling combined with needling plus blood-letting puncturing and cupping and simple needling plus blood-letting puncturing and cupping are effective in treating shoulder-hand syndrome after apoplexy, and the former is better than the latter. 展开更多
关键词 APOPLEXY Shoulder-hand Syndrome Kinetic Needling Blood-letting Puncturing and Cupping (b-ipc)
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部