面向机载探测系统大视场光学成像的需求,开展了大视场光学成像系统光学设计、自由曲面光学元件超精密加工、形位误差同步检测以及系统集成与成像实验研究。首先,采用视场扩展法进行大视场自由曲面离轴反射光学系统的设计;其次,进行铝合...面向机载探测系统大视场光学成像的需求,开展了大视场光学成像系统光学设计、自由曲面光学元件超精密加工、形位误差同步检测以及系统集成与成像实验研究。首先,采用视场扩展法进行大视场自由曲面离轴反射光学系统的设计;其次,进行铝合金自由曲面反射镜纳米精度加工和高频抑制工艺探索,并实现了基于计算全息元件的自由曲面形位高精度检测;最后,进行了光学系统的装调集成与成像实验。结果表明,系统的视场角为30°×5°,全视场光学传递函数值大于0.7,接近衍射极限,最大像元均方根半径为2.075μm,自由曲面光学元件的面形精度均方根(Root Mean Square,RMS)值优于20 nm,位置精度优于1μm,装配集成后能够满足大视场高分辨的场景使用要求,同时具备稳定可靠和快响制造等特点。展开更多
Design method and procedures of computer-generated hologram （CGH） used for aspheric test are in- troduced in detail. For CGH phase calculation, virtual medium which has zero refractive index at given wavelength is u...Design method and procedures of computer-generated hologram （CGH） used for aspheric test are in- troduced in detail. For CGH phase calculation, virtual medium which has zero refractive index at given wavelength is used to model ideal aspheric wavefront. Reflective Fresnel zones located in a ring area concentric to the CGH structure is designed to reduce or eliminate alignment errors. Substrate figure error, pattern distortion, etching and duty cycle variations that influence the reconstructed wavefront are quantitatively analyzed in theory and corresponding error equations are obtained to guide the tolerance distribution during CGH fabricating. A design example is given and the uncertainty of t achieves λ20.展开更多
The singularity at distance r → 0 at the center of a spherically symmetric non-rotating, uncharged mass of radius R, is considered here. Under inverse square law force, the Schwarzschild metric, needs to be modified,...The singularity at distance r → 0 at the center of a spherically symmetric non-rotating, uncharged mass of radius R, is considered here. Under inverse square law force, the Schwarzschild metric, needs to be modified, to include Newton’s Shell Theorem (NST). By including NST for r, both Schwarzschild singularity at r = 2GM/c2 and at r → 0 singularities are removed from the metric. Near R → 0, the question of maximal density is considered based on Schwarzschild’s modified metric, and compared to the quantum limit of maximal mass density put by Planck’s quantum-based universal units. It is asserted, that General relativity, when combined with Planck’s universal units, inevitably leads to quantization of gravity.展开更多
Acute lymphoblastic leukemia (ALL) is the most common malignancy in children, with the majority of cases being of precursor B-cell phenoltype. Conventional cytogenetic analysis plays an important role in the diagnosis...Acute lymphoblastic leukemia (ALL) is the most common malignancy in children, with the majority of cases being of precursor B-cell phenoltype. Conventional cytogenetic analysis plays an important role in the diagnosis of B-cell ALL, identifying characteristic chromosomal abnormalities associated with a given prognosis therein facilitating optimized treatment. The more recent introduction of microarray technology to the analysis of B-cell ALL has afforded both higher resolution for the detection of known abnormalities and an ability to identify novel copy number abnormalities (CNAs) with potential clinical relevance. In the current study, microarray analysis was performed on 20 cytogenetically abnormal B-cell ALL cases (10 pediatric and 10 adult), while a novel microarray-based balanced-translocation detection methodology (translocation CGH or tCGH) was applied to that subset of cases with a known or suspected recurrent balanced translocation. Standard microarray analysis identified that CNAs was not detected by previous conventional cytogenetics in 75% (15/20) cases. tCGH identified 9/9 (100%) balanced translocations defining BCR/ABL1 (x4), ETV6/RUNX1 (x3), and MLL/AFF1 (x2) breakpoints with high resolution. The results illustrate the improved molecular detail afforded by these technologies and a comparison of translocation breakpoints, CNAs and patient age offers new insights into tumor biology with potential prognostic significance.展开更多
为鉴定影响蛋壳色素含量的基因组拷贝数变异,试验以长顺绿壳蛋鸡为研究对象,依据蛋壳表型设置2个试验组:B2 vs B1和B3 vs B1,通过CGH芯片全基因组检测筛选和Ac⁃cuCopyTM多重基因拷贝数检测验证,在全基因组范围内进行鉴定,选择其中12个...为鉴定影响蛋壳色素含量的基因组拷贝数变异,试验以长顺绿壳蛋鸡为研究对象,依据蛋壳表型设置2个试验组:B2 vs B1和B3 vs B1,通过CGH芯片全基因组检测筛选和Ac⁃cuCopyTM多重基因拷贝数检测验证,在全基因组范围内进行鉴定,选择其中12个注释功能基因开展关联验证。结果显示:两个试验组分别检测到绿壳表型差异关联区域22个和24个;腺苷酸环化酶10(ADCY10)基因拷贝数变异与原卟啉色素含量呈极显著负相关(R=-0.424,P=0.001),与胆绿素色素含量呈显著正相关(R=0.299,P=0.018)。研究提示,ADCY10基因可能是影响蛋壳中不同色素含量的关键基因之一。展开更多
文摘面向机载探测系统大视场光学成像的需求,开展了大视场光学成像系统光学设计、自由曲面光学元件超精密加工、形位误差同步检测以及系统集成与成像实验研究。首先,采用视场扩展法进行大视场自由曲面离轴反射光学系统的设计;其次,进行铝合金自由曲面反射镜纳米精度加工和高频抑制工艺探索,并实现了基于计算全息元件的自由曲面形位高精度检测;最后,进行了光学系统的装调集成与成像实验。结果表明,系统的视场角为30°×5°,全视场光学传递函数值大于0.7,接近衍射极限,最大像元均方根半径为2.075μm,自由曲面光学元件的面形精度均方根(Root Mean Square,RMS)值优于20 nm,位置精度优于1μm,装配集成后能够满足大视场高分辨的场景使用要求,同时具备稳定可靠和快响制造等特点。
基金the Ministerial Level Foundation (4645103030401)
文摘Design method and procedures of computer-generated hologram （CGH） used for aspheric test are in- troduced in detail. For CGH phase calculation, virtual medium which has zero refractive index at given wavelength is used to model ideal aspheric wavefront. Reflective Fresnel zones located in a ring area concentric to the CGH structure is designed to reduce or eliminate alignment errors. Substrate figure error, pattern distortion, etching and duty cycle variations that influence the reconstructed wavefront are quantitatively analyzed in theory and corresponding error equations are obtained to guide the tolerance distribution during CGH fabricating. A design example is given and the uncertainty of t achieves λ20.
文摘The singularity at distance r → 0 at the center of a spherically symmetric non-rotating, uncharged mass of radius R, is considered here. Under inverse square law force, the Schwarzschild metric, needs to be modified, to include Newton’s Shell Theorem (NST). By including NST for r, both Schwarzschild singularity at r = 2GM/c2 and at r → 0 singularities are removed from the metric. Near R → 0, the question of maximal density is considered based on Schwarzschild’s modified metric, and compared to the quantum limit of maximal mass density put by Planck’s quantum-based universal units. It is asserted, that General relativity, when combined with Planck’s universal units, inevitably leads to quantization of gravity.
文摘Acute lymphoblastic leukemia (ALL) is the most common malignancy in children, with the majority of cases being of precursor B-cell phenoltype. Conventional cytogenetic analysis plays an important role in the diagnosis of B-cell ALL, identifying characteristic chromosomal abnormalities associated with a given prognosis therein facilitating optimized treatment. The more recent introduction of microarray technology to the analysis of B-cell ALL has afforded both higher resolution for the detection of known abnormalities and an ability to identify novel copy number abnormalities (CNAs) with potential clinical relevance. In the current study, microarray analysis was performed on 20 cytogenetically abnormal B-cell ALL cases (10 pediatric and 10 adult), while a novel microarray-based balanced-translocation detection methodology (translocation CGH or tCGH) was applied to that subset of cases with a known or suspected recurrent balanced translocation. Standard microarray analysis identified that CNAs was not detected by previous conventional cytogenetics in 75% (15/20) cases. tCGH identified 9/9 (100%) balanced translocations defining BCR/ABL1 (x4), ETV6/RUNX1 (x3), and MLL/AFF1 (x2) breakpoints with high resolution. The results illustrate the improved molecular detail afforded by these technologies and a comparison of translocation breakpoints, CNAs and patient age offers new insights into tumor biology with potential prognostic significance.
文摘为鉴定影响蛋壳色素含量的基因组拷贝数变异,试验以长顺绿壳蛋鸡为研究对象,依据蛋壳表型设置2个试验组:B2 vs B1和B3 vs B1,通过CGH芯片全基因组检测筛选和Ac⁃cuCopyTM多重基因拷贝数检测验证,在全基因组范围内进行鉴定,选择其中12个注释功能基因开展关联验证。结果显示:两个试验组分别检测到绿壳表型差异关联区域22个和24个;腺苷酸环化酶10(ADCY10)基因拷贝数变异与原卟啉色素含量呈极显著负相关(R=-0.424,P=0.001),与胆绿素色素含量呈显著正相关(R=0.299,P=0.018)。研究提示,ADCY10基因可能是影响蛋壳中不同色素含量的关键基因之一。
