In the present study, a japonica rice ( Oryza sativa L. ) variety Nipponbare, an indica variety 9311 and a set of chromosome segment substitution lines (CSSLs) which were generated using Nipponbare as the recipien...In the present study, a japonica rice ( Oryza sativa L. ) variety Nipponbare, an indica variety 9311 and a set of chromosome segment substitution lines (CSSLs) which were generated using Nipponbare as the recipient parent and 9311 as the donor parent were used as the experimental materials. The CSSLs were grown in 2012 (normal temperature condition) and 2013 (high temperature condition) in Yangzhou, Jiangsu, and were used to map the quantitative trait loci (QTLs) for heat tolerance, based on the heat tolerance index [ (The seed setting rate under normal temperature condition -The seed setting rate under high temper- ature condition) / The seed setting rate under normal temperature condition]. As a result, three QTLs related to heat tolerance in rice were mapped on chromo- somes 2, 4 and 12, respectively. They had LOD (logarithm of rntds) scores of 2.56, 4.02 and 2.79, and contributian rates of 4.95%, 7.99% and 5.44%. Among them, qHT12.1 showed positive effect, while qHT2.1 and qHT4. t showed negative effect on heat tolerance. The results lay a foundation for the fine mapping and cloning of the QTLs and genes related to heat tolerance, and for the breeding of heat-tolerant rice varieties.展开更多
Chromosome segment substitution lines(CSSLs) are useful for the precise mapping of quantitative trait loci(QTLs) and dissection of the genetic basis of complex traits.In this study,two whole-genome sequenced rice ...Chromosome segment substitution lines(CSSLs) are useful for the precise mapping of quantitative trait loci(QTLs) and dissection of the genetic basis of complex traits.In this study,two whole-genome sequenced rice cultivars,the japonica Nipponbare and indica 9311 were used as recipient and donor,respectively.A population with 57 CSSLs was developed after crossing and back-crossing assisted by molecular markers, and genotypes were identified using a high-throughput resequencing strategy.Detailed graphical genotypes of 38 lines were constructed based on resequencing data.These CSSLs had a total of 95 substituted segments derived from indica 9311,with an average of about 2.5 segments per CSSL and eight segments per chromosome,and covered about 87.4%of the rice whole genome.A multiple linear regression QTL analysis mapped four QTLs for 1000-grain weight.The largest-effect QTL was located in a region on chromosome 5 that contained a cloned major QTL GW5/qSW5 for grain size in rice.These CSSLs with a background of Nipponbare may provide powerful tools for future whole-genome展开更多
基金Supported by the National Natural Science Foundation of China(31561143008,31401354)Jiangsu Provincial Natural Science Foundation(BK20140484)Doctoral Fund of Ministry of Education of China(20133250120001)
文摘In the present study, a japonica rice ( Oryza sativa L. ) variety Nipponbare, an indica variety 9311 and a set of chromosome segment substitution lines (CSSLs) which were generated using Nipponbare as the recipient parent and 9311 as the donor parent were used as the experimental materials. The CSSLs were grown in 2012 (normal temperature condition) and 2013 (high temperature condition) in Yangzhou, Jiangsu, and were used to map the quantitative trait loci (QTLs) for heat tolerance, based on the heat tolerance index [ (The seed setting rate under normal temperature condition -The seed setting rate under high temper- ature condition) / The seed setting rate under normal temperature condition]. As a result, three QTLs related to heat tolerance in rice were mapped on chromo- somes 2, 4 and 12, respectively. They had LOD (logarithm of rntds) scores of 2.56, 4.02 and 2.79, and contributian rates of 4.95%, 7.99% and 5.44%. Among them, qHT12.1 showed positive effect, while qHT2.1 and qHT4. t showed negative effect on heat tolerance. The results lay a foundation for the fine mapping and cloning of the QTLs and genes related to heat tolerance, and for the breeding of heat-tolerant rice varieties.
基金supported in part by the National Basic Research Program(Nos.2012CB944803 and 2011CB 100202)the National Natural Science Foundation(No.31071383)+1 种基金the National Special Program for Transgenic Research(No. 2009ZX08009-008B)the Priority Academic Program Development from Jiangsu Government of China
文摘Chromosome segment substitution lines(CSSLs) are useful for the precise mapping of quantitative trait loci(QTLs) and dissection of the genetic basis of complex traits.In this study,two whole-genome sequenced rice cultivars,the japonica Nipponbare and indica 9311 were used as recipient and donor,respectively.A population with 57 CSSLs was developed after crossing and back-crossing assisted by molecular markers, and genotypes were identified using a high-throughput resequencing strategy.Detailed graphical genotypes of 38 lines were constructed based on resequencing data.These CSSLs had a total of 95 substituted segments derived from indica 9311,with an average of about 2.5 segments per CSSL and eight segments per chromosome,and covered about 87.4%of the rice whole genome.A multiple linear regression QTL analysis mapped four QTLs for 1000-grain weight.The largest-effect QTL was located in a region on chromosome 5 that contained a cloned major QTL GW5/qSW5 for grain size in rice.These CSSLs with a background of Nipponbare may provide powerful tools for future whole-genome