Effect of Cooking on Amylose Contents of Mungbean （Phaseolus aureus） and Chickpea （Cicer arietinum L.） Varieties

Keeping in view the importance of amylose for various food industries as well as its importance on domestic level, the current study was conducted to analyze the amylose content of pulses of common use e.g. mungbean （NM-92, NM-98 and Ramazan） and kabuli type chickpea （CMNKI0-99, CMNK452-2 and Hassan 2k） varieties and quantifying the influence of cooking （boiling） on the amylose contents of pulses. The data revealed that moisture and arnylose content of uncooked mungbean varieties ranged 9.01-9.47% and 20.06-22.26% respectively. The moisture and amylose content of Kabuli type chickpea varieties varied 8.52-8.79% and 20.25-22.83% respectively. Moisture content of mungbean （64.16-66.08%） as well as chickpea （55.56-61.52%） varieties increased after cooking. For all the three varieties of mungbean the observed cooking time was 11 minutes. The maximum value of amylose content was assayed for NM-98 （20.74%） followed by Ramzan （20.24%）. The cooking time of Kabuli type chickpea varieties CMNK-452-2 and CMNK-10-99 was 60 min. Hassan 2K observed more cooking time （90 min） and highest value of amylose content in cooked （19.01%） samples. The effect of cooking/varietals on sensory evaluation （appearance, flavor, texture and overall acceptability） of mungbean varieties was statistically non significant attributes. Among chickpea varieties, Hassan 2K was ranked lowest due to its small size and hard texture and the sensory attributes were statistically significant （P 〈 0.05） as compared to the other two varieties.

EMS诱变对鹰嘴紫云英种子萌发及幼苗表型特征的影响

【目的】筛选适宜鹰嘴紫云英的EMS诱变条件。【方法】以鹰嘴紫云英品种甘绿2号种子为试验材料,采用不同浓度(0、0.2%、0.4%、0.6%、0.8%)的甲基磺酸乙酯(EMS)进行不同时间(2、4、6、8 h)的诱变处理,探究EMS对鹰嘴紫云英种子萌发特征及表型变化的影响。【结果】低浓度和短时间EMS处理促进鹰嘴紫云英种子的萌发,随EMS浓度升高和诱变时间的增加,对鹰嘴紫云英种子萌发产生抑制作用。种子浸出液电导率随诱变条件的加深呈上升趋势,在2~6 h时,萌发期发芽率、发芽势、幼苗苗长和根长均随处理时间和诱变浓度的增加呈先升高后降低的趋势,在8 h处理下,随EMS浓度的增加呈逐渐降低趋势,EMS浓度在0.8%时,种子发芽率和发芽势均达到最低,较对照分别降低了54.45%和56.67%;经形态学筛选,发现有鹰嘴紫云英幼苗表现出幼苗矮小、停滞生长、叶片黄化、宽叶、窄叶、叶片发紫、诸多病斑等特殊变异性状。【结论】0.8%EMS处理6 h可作为最佳诱变组合;幼苗的生长随诱变时间和浓度增加而明显受到抑制,且表型变异类型也逐步增多。

Interaction between seed size and NaCl on germination and early seedling growth of some Turkish cultivars of chickpea(Cicer arietinum L.)

Chickpea is an important food legume crop of Turkey and is largely grown for human consumption on low moisture or salt-affected soils. The objective of the study was to find the effects of NaCl stress at electrical conductivities of 4.5, 8.6, 12.7 and 16.3 dS/m and seed sizes （7, 8 and 9 mm） on germination and early seedling growth of three popular chickpea cultivars （AKN-97, Gokce and Uzunlu-99）. Mean frequency of germination, germination time, germination index, root length, shoot length and seedling fresh weight showed seed size-dependent responses ofcultivars to salt stress. In general, small seeds germinated and grew more rapidly compared to medium and large seeds of the same cultivars against all levels of salt stress, with the best results in cultivar Uzunlu-99. No effect of NaCl treatments was observed on frequency of germination; however, a drastic decrease in early seedling growth was recorded at increased NaC1 concentrations. Regression analysis results showed a significantly positive relationship （P〈0.01） between seed size and mean germination time, whereas a significantly negative relationship was recorded between seed size and germination index, root length, shoot length. Moreover, linear regression values apparently confirmed that increased seed size in each cultivar affected decreased germination index, root and shoot lengths with enhanced mean germination time. Thus, it was concluded that the use of small seeds could considerably reduce the production costs of chickpea in salt-affected soils.

Yield and Water Productivity of Chickpea (<i>Cicer arietinum</i>L.) as Influenced by Different Irrigation Regimes and Varieties under Semi Desert Climatic Conditions of Sudan

A field experiment was conducted at Hudeiba Research Station Farm, located at Ed-Damer, Sudan during 2011/2012 and 2012/2013 winter seasons to investigate the effect of different irrigation regimes and varieties on chickpea (Cicer arietinum L.) yield, yield components and water productivity. The treatments include three irrigation regimes;irrigation every 10 days (I1 = full irrigation), irrigation every 15 days (I2 = moderate stress) and irrigation every 20 days (I3 = severe stress) and two varieties (Borgieg and Wad Hamid). The treatments were arranged in factorial randomized complete block design (RCBD) with 3 replications. Irrigation water being applied, grain yield, yield components (number of pods per plant, number of seeds per pod and the 100 seeds weight) and crop water productivity (CWP) and irrigation water productivity (IWP) were recorded. Results showed that the number of pods per plant, number of seeds per pod, 100-seeds weight, grain yield and irrigation water applied were significantly (p ≤ 0.001) affected by irrigation regimes. The highest values of these traits obtained with full irrigation, whereas the lowest values were recorded under severe water stress conditions. Results also indicated that, moderate and severe water stress regimes saved irrigation water by 24% and 32%, respectively compared with full irrigation. This study indicated that treatment I1 which was irrigated every 10-days did not produce the highest IWP, while treatment I2 which irrigated every 15-days gave the highest IWP. The lowest IWP occurred at severe water stress regime (I3). It could be concluded that moderate water stress might be adopted. Contrarily, the adoption of severe water stressed that produce high water savings would lead to yield losses that might be economically not acceptable. The late maturing chickpea variety of Borgieg significantly (p ≤ 0.05) out-yielded the early maturing variety Wad Hamid by 11%. Borgieg displayed the highest values of CWP and IWP.

Effect of Cultivar, Irrigation and Nitrogen Fertilization on Chickpea (<i>Cicer arietinum</i>L.) Productivity

A 2-year field study was conducted in northern Greece to investigate the effect of nitrogen fertilization and irrigation on productivity of three Greek chickpea varieties (“Amorgos” “Serifos”, “Andros”). Chickpea, grown under irrigation regime (30 + 30 mm of water) and fertilized with 50 kg·N·ha-1 before planting and with 40 kg·N·ha-1 at blossom growth stage, produced more total dry biomass and seed yield as compared with that grown under non-irrigated conditions and fertilized with 50 kg·N·ha-1 before planting only. In particular, irrigation and nitrogen fertilization at blossom growth stage increased total dry weight of chickpea by 18.3% and 18.5%, respectively, as compared with that of non-irrigated and fertilized with N before planting. The corresponding increase of seed yield was 30.5% and 20%, respectively. The total dry biomass of “Amorgos” was 10% and 13% greater than that of “Serifos” and “Andros”, while its respective seed yield increase was 5% and 16%. Finally, the quantum yield of photosystem II of chickpea was not affected by irrigation or fertilization. These results indicated that nitrogen fertilization at blossom growth stage combined with irrigation increased seed yield of all chickpea varieties, whereas the same treatments did not have any effect on plant quantum yield of photosystem II.

Rhizogenesis of Two Species Fabaceae: <i>Cicer arietinum</i>L. and <i>Pisum sativum</i>L.

Our work of comparative study on the biomorphological, rhizogenic level of Chickpea (Cicer arietinum) and Pea (Pisum sativum), includes successively the following results: The in-vitro culture done, showed strains, which can reach 6.5 mm in the medium Nutrient agar (NA) for Cicer arietinum 6 mm of strain were recorded in the PDA culture medium for Pisum sativum. The best strain is obtained in temperature of 20&#8451;(ambient). According to the results of the rhizogenesis, we notice that the nutrient agar (NA) medium responds better that the PDA medium for Cicer arietinum. Meanwhile, the medium PDA brings a better reaction (response) compared with the nutrient agar medium concerning Pisum sativum.

Gene Expression Profiling during Wilting in Chickpea Caused by <i>Fusarium oxysporum</i>F. sp. <i>Ciceri</i>

Fusarium oxysporum f. sp. ciceri (Foc), one of the most important fungal pathogen of chickpea, is a constant threat to this crop plant. In the present study gene expression analysis of chickpea roots during Foc infection was performed using various approaches. cDNAs derived from total mRNA during infection process of susceptible (JG62)and resistant (Digvijay) cultivars, were amplified using random oligonucleotides. Sequence characterization of differentially expressed transcripts revealed their homology with many plant genes essential for various metabolic functions including defense. Further, expression patterns of specific candidate gene transcripts were analyzed in the Foc inoculated and uninoculated resistant and susceptible chickpea cultivars, on day 6 of infection. Semiquantitative RT-PCR analysis of defense related genes was performed using gene specific oligonucleotides in resistant and susceptible chickpea cultivars. The expression of fungal pathogenesis related genes and their race specific response was determined throughout the course of chickpea-Foc interaction. Temporal expression and race specific response of plant defense related and fungal virulence genes were studied in the resistant and susceptible cultivars of chickpea inoculated with three races of Foc highlighting the host-pathogen interactions. Few genes, involved in chickpea defense against Fusarium wilt which were not reported previously were unveiled in this study.

A Kunitz trypsin inhibitor from chickpea (<i>Cicer arietinum</i>L.) that exerts an antimicrobial effect on Fusarium oxysporum f.sp. ciceris

Fusarium oxysporum f.sp. ciceris (Foc) is one of the most important fungal pathogens of chickpea and is regarded as a constant threat in tropical and subtropical countries. In order to correlate Fusarium wilt resistance/susceptibility in Cicer arietinum to the presence or absence of trypsin inhibitor (TI) in the crude extract, trypsin inhibitory assay (TIA) and in vitro activity of TI against Foc were studied. In the present study, a 20 kDa trypsin inhibitor was purified from Fusarium wilt resistant cultivar (viz. JG 2001-12) by ammonium sulfate precipitation, dialysis and chromatographies with Sephadex G-100 and Diethyl aminoethyl cellulose (DEAE-cellulose-52) ion-exchange column. Results of pathogenecity assay were found to be in correlation to the trypsin inhibitor assay where the Fusarium wilt resistant cultivar showed high trypsin inhibitory activity (99%) in the presence of trypsin enzyme using both natural and synthetic substrates. Preliminary studies using crude extracts of JG 2001-12 showed a decrease in radial growth of Foc. A 45%-82% reduction in conidium germination at 20 μg&middotmL-1?Cicer arietinum trypsin inhibitor (CaTI) concentration was observed, thereby, indicating the use of CaTI in suppression of pathogen and in its deployment through transgenic plants for the management of Fusarium wilt.

Variation in Thermal time model Parameters Between Two Contrasting Chickpea (<i>Cicer arietinum</i>) cultivars

A laboratory experiment was carried out to determine the effect of different constant temperatures on germination and early seedling establishment and to study the variation among parameters of thermal time model parameters for two contrasting chickpea cultivars . Seeds were subjected to six constant temperatures from 10 o C to 35 o C . A complete randomized design was used with four replication. Analysis of variance showed significant differences among treatments for all characters studied. The final germination percentage significantly increased with increasing temperature up to 25 ° C, and thereafter there was a sharp decrease in final germination at 30 ° and 35 ° C. Desi type cultivar (small seeded) “Jabel Marra” significantly exhibited higher final germination percentage and lower germination rate compared with the kabui type cultivar “Shendi” at all temperatures. The median (θ T(50) ) of the thermal time was significantly differ between the two chickpea cultivars. The large seeded cultivars (shendi) recorded significantly higher median thermal time than the small seeded cultivars (Jabel Marra). The results also revealed a significant differences between the two cultivars in all thermal time model parameters. The small seeded cultivar (Jabel Marra) scored lower total dry matter and temperature tolerance index (TTI) compared to the large seeded cultivar (Shendi) at all temperatures studied.

不同处理方式对鹰嘴紫云英种子萌发和生长的影响

为探索打破鹰嘴紫云英种子硬实、促进种子萌发的方法,本研究以鹰嘴紫云英甘绿2号为试验材料,采用恒温水浴、物理破除种皮和浓硫酸浸泡等3种种子处理方法,测定鹰嘴紫云英种子的发芽率、发芽势、根长和苗长等各项萌发指标和生长指标。结果表明,3种处理方法对鹰嘴紫云英种子的萌发均有促进作用,综合各萌发指标和幼苗生长指标,表现为划破种皮处理12 h>浓硫酸处理15 min>热水浴处理1 min,但划破种皮较为繁琐耗时,不适用于大型农业生产。综合考虑,以浓硫酸浸泡15 min为最优处理方式。

人工老化对鹰嘴豆种子发芽和膜透性的影响

以鹰嘴豆种子为研究材料,分别进行12 h、24 h、36 h、48 h、60 h、72 h、84 h及96 h 8个人工老化处理(40℃、相对湿度100%),研究老化过程中种子的发芽率、发芽指数、活力指数、苗长、根长、电导率及可溶性糖的变化趋势,分析人工老化处理对鹰嘴豆种子膜透性的影响。结果表明,鹰嘴豆种子在老化处理后,发芽率、发芽指数先急剧下降(12 h)而后缓慢下降,浸种液电导率先缓慢上升而后急剧上升(48 h),而可溶性糖含量先急剧上升(12 h)后缓慢上升再略有下降(96 h)。不同老化程度种子的电导率与发芽指数和发芽率均呈显著正相关(P<0.05),发芽率与发芽指数呈显著正相关(P<0.05),可溶性糖与活力指数呈极显著正相关(P<0.01),其他各指标间无显著相关性。

鹰嘴豆种质资源农艺性状遗传多样性分析

以100份鹰嘴豆种质资源为材料,应用聚类分析和主成分分析方法,对15个主要农艺性状的遗传多样性进行分析。结果表明,参试材料存在广泛的遗传多样性。其中,多样性指数最高的是株高,其次是百粒重;性状变异系数最大的是单株荚数,其次是单株粒重;基于各种质间形态标记的遗传差异,将100份鹰嘴豆种质聚类并划分为4大类群。第Ⅰ类群可作为选育丰产中粒型和株高适中的品种,第Ⅱ类群可作为选育矮秆耐密及特异粒色(型)品种,第Ⅲ类群丰产性较差可作为选育子粒球型、光滑的品种,第Ⅳ类群可作为选育大粒型、适宜机械化收获的品种。9个数量性状的主成分分析结果表明,前4个主成分累计贡献率达73.91%,各主成分性状载荷值反映了主要数量性状的育种选择潜力。综合分析种质资源农艺性状,为鹰嘴豆的有效利用提供一定的科学依据。

鹰咀豆根系分泌物的分离鉴定及典型分泌物苯甲醛的化感效应

运用CH2Cl2提取水培35d鹰咀豆的根系分泌物,并通过气相色谱和质谱联用技术(GC MS)进行分离鉴定,对典型分泌物苯甲醛的生物学效应通过发芽试验进行了测试。结果表明,鹰咀豆的根分泌物主要包括有机酸、酚、烃类、酯、酮类、醇、酰胺、苯、醛和噻唑等;苯甲醛是一种他感作用强于自毒作用的化感物质,对2种幼苗组成的复合系统的负效应小于对单作系统的负效应。

鹰嘴豆种子胰蛋白酶抑制剂的分离纯化与鉴定

为了寻找具有药物作用的天然胰蛋白酶抑制物,采用硫酸铵分级沉淀、离子交换层析(DEAE-纤维素52)及Sephadex G-100凝胶层析等方法,从鹰嘴豆种子中分离出一种鹰嘴豆胰蛋白酶抑制剂(CPTI).研究表明:CPTI对胰蛋白酶有较强的抑制作用,抑制率达80%,而对胰凝乳蛋白酶抑制作用较弱,抑制率为32%,对胃蛋白酶、木瓜蛋白酶及枯草杆菌蛋白酶均无抑制作用;用SDS-PAGE测得CPTI近似分子质量为25.7 kD;CPTI具有较高的热稳定性,在100℃下加热60min,对胰蛋白酶活性仍保持78%抑制率;Lineveaer-Burk作图得知该抑制剂属竞争性抑制类型.动力学测定显示,来自鹰嘴豆中的CPTI对胰蛋白酶的抑制作用常数(Ki)为3.99×10-7 mol/L.

富钒鹰嘴豆芽对糖尿病大鼠血糖和血脂的影响

目的:研究富钒鹰嘴豆芽降糖作用及可能机制。方法:以链脲佐菌素诱导形成糖尿病大鼠模型,饲喂不同浓度的富钒鹰嘴豆芽4周,观察大鼠血糖和血脂等的变化。结果:与正常对照组比较,糖尿病模型大鼠的血糖升高了4倍,血浆胰岛素降低了57.63%、糖化血红蛋白(GHb)升高了42.28%,谷丙转氨酶、谷草转氨酶和血浆脂质水平明显升高。而分别饲喂不同浓度富钒鹰嘴豆芽4周后,糖尿病大鼠的上述指标都得到改善,其中以中等剂量(100μg/ml)富钒鹰嘴豆芽治疗组的效果最明显:血糖水平降低了58.60%(P<0.05);血浆胰岛素水平明显增高(P<0.05)且接近正常;GHb降低了32.81%,血浆低密度脂蛋白、甘油三酯和总胆固醇明显下降(P<0.05),谷丙转氨酶和谷草转氨酶分别降低了39.98%和41.37%。而葡萄糖耐量得到显著改善,2h的血糖值降低了38.46%。结论:富钒鹰嘴豆芽能阻止糖尿病大鼠高血糖的进展和血浆脂质水平的改变;这种钒的有机复合物形式达到了减毒增效的目的,具有较佳的应用前景。

紫外分光光度法测定鹰嘴豆和豆芽中异黄酮含量的研究

目的建立一种检测鹰嘴豆异黄酮含量的快速分析方法，并检测鹰嘴豆和豆芽中异黄酮的含量。方法采用紫外分光光度法，检测波长为260．9nm，测定鹰嘴豆及其豆芽中异黄酮的含量测定。结果鹰嘴豆芽素A在2—30μg／ml范围内吸光度与浓度呈良好的线性关系，以黄酮总含量测定结果重复性RSD=1．95％，加样回收率为100．18％。结论为鹰嘴豆提取物及制品提供一种简单、可靠、有效的测定方法。

鹰嘴紫云英甲硫氨酸抗性系原生质体培养及植株再生

本研究建立了鹰嘴紫云英(AstragaluscicerL.)甲硫氨酸抗性系原生质体再生植株的实验体系。以茎切段诱导的松软愈伤组织为材料,通过酶法游离出大量有活力的原生质体。原生质体经培养持续细胞分裂形成了愈伤组织,并分化出再生苗。比较了不同培养基、培养密度对原生质体形成细胞分裂和再生的影响。结果表明,原生质体以2×105个/ml的植板密度,在附加2.0mg/L2,4-二氯苯氧乙酸(2,4-D)、0.2mg/L6-苄氨基嘌呤(6-BA)、200mg/L水解酪蛋白、2%蔗糖和0.3mol/L甘露醇DPD培养基中培养后,其分裂频率达38.3%。原生质体培养形成的愈伤组织仍具有对甲硫氨酸的抗性。转移到附加10mg/LKT、0.5mg/LNAA的MS分化培养基上,获得大量的再生苗。

GC-MS应用于鹰嘴豆中弱极性化学成分的研究(英文)

应用气相色谱-质谱分析技术,首次对鹰嘴豆中弱极性成分进行研究。从鹰嘴豆弱极性萃取部位中鉴定了22个化合物,以脂肪酸为主要成分。脂肪酸含量(质量分数)高达81.25%,其中9-十八烯酸和8,11-十八碳二烯酸的含量分别占总量的28.00%和38.60%。

果园种草对土壤及果树的影响

在老龄果园套种优良牧草鸭等（Dactylisglomerata）和鹰嘴紫云英（Astragaluscicer）。经3年试验，研究结果表明，果园套种鸭茅和鹰嘴紫云荚，具有蓄水保墒，提高土壤肥力，促进果树生长发育，增加果实产量，改善果实品质等明显效应。尤其鸭茅具有耐前性强，覆盖度大，生物学量高，再生性强等优点，且易于栽培，可在老龄果园中大面积推广。

鹰嘴豆化学成分研究

目的:研究鹰嘴豆的化学成分,从中寻找具有生物活性的天然化合物。方法:采用不同体积分数的乙醇对鹰嘴豆干燥种子及鹰嘴豆发芽种子进行提取,经硅胶、大孔树脂D101、葡聚糖Sephadex LH-20色谱手段分离纯化,通过NMR,MS等波谱分析手段鉴定化合物结构。结果:分离到9个化合物,分别鉴定为3-羟基-齐墩果-12-烯(1),鹰嘴豆芽素A-7-O-β-D-葡萄糖苷(2),脑苷(3),1-乙基-α-L-半乳糖苷(4),尿苷(5),腺苷(6),色氨酸(7),鹰嘴豆芽素A(8),芒柄花素(9)。结论:1,3,4,6,7均为首次从鹰嘴豆属植物中分离到。