报道了中国希瓦氏菌D14 T 的Fe(Ⅲ)还原特性,研究了溶氧浓度、光照强度、温度、pH等条件对菌株Fe(Ⅲ)还原的影响。结果发现,随着培养基中Fe(Ⅲ)浓度的提高,菌株D14 T 的Fe(Ⅲ)还原速率相应降低;氧气和光照对Fe(Ⅲ)还原有一定的抑制作用...报道了中国希瓦氏菌D14 T 的Fe(Ⅲ)还原特性,研究了溶氧浓度、光照强度、温度、pH等条件对菌株Fe(Ⅲ)还原的影响。结果发现,随着培养基中Fe(Ⅲ)浓度的提高,菌株D14 T 的Fe(Ⅲ)还原速率相应降低;氧气和光照对Fe(Ⅲ)还原有一定的抑制作用;菌株还原Fe(Ⅲ)的最适反应温度为37℃;在反应起始pH6 0 - 10 0的条件下菌株可进行Fe(Ⅲ)还原。对不同形态Fe(Ⅲ)还原特性的研究结果表明,Fe(Ⅲ)的溶解度越高越有利于还原反应的进行。采用SDS和OGP这两种蛋白变性剂对Fe(Ⅲ)还原蛋白进行初步定位的结果表明,参与Fe(Ⅲ)还原的蛋白主要位于细胞可溶性外周蛋白。在同时含有偶氮染料和Fe(Ⅲ)的条件下,菌株D14 T 的偶氮染料脱色率和Fe(Ⅲ)展开更多
AIM. To establish a kind of standardization of the clinical chemotherapeutic prototypes for unresectable hepatocellular carcinomas （HCC）. METHODS： 10-Hydroxycamptothecin （HCPT） was applied through transcatheter a...AIM. To establish a kind of standardization of the clinical chemotherapeutic prototypes for unresectable hepatocellular carcinomas （HCC）. METHODS： 10-Hydroxycamptothecin （HCPT） was applied through transcatheter arterial embolization （TAE） to HCC patients who were categorized into three groups： （1） test group： treatment with HCPT twice （HCPT dl and 14） through TAE and portal venous embolization. （2） Control Ⅰ： treatment with anticancer drugs without HCPT. （3） Control Ⅱ： treatment with HCPT as a major component in anticancer drugs once （HCPT dl）. A set of comparisons between test groups and control Ⅰ and Ⅱ groups were performed before and after the treatment to study the effectiveness of each treatment, in terms of tumor volumes, dynamic variations in serum alpha-fetoprotein （AFP）, gamma-glutamyl transferase hepatoma-specific band （GGT-Ⅱ）, patient survival and adverse events. RESULTS： The general effectiveness rate of the test group reached 62.1% （72/116）, remarkably higher than that of control Ⅰ （32.1%, 40/124） and control Ⅱ （54.7%, 47/56）, （P〈0.01 and P〈0.05, respectively）. Especially, the reduction rate or disappearance of the portal vein tumor emboli was as high as 88.4% （61/69） in the test group, in contrast with 13.9% （10/72） in control Ⅰ and 35.9% （18/51） in control Ⅱ （P〈0.01 and P〈0.01, respectively）. After treatment, AFP decreased or turned to negative levels at 52.3% （34/65） in control Ⅰ, 67.3% （35/52） in control Ⅱ, and 96.8% （60/62） in the test group. Also GGT-Ⅱ declined or became negative at 37.8% （28/74） in control Ⅰ, 69.5% （57/82） in control Ⅱ, and 94.7% （89/94） in test group （P〈0.01 and P〈0.05, respectively）. CONCLUSION： We have designed a good protocol （test group） to treat HCC with excellent advantages of high efficiency, low cost, low toxicity and low adverse events and easy application. It could be recommended as one of the standardizations for HCC treatment in clinical practice.展开更多
Strigolactones （SLs） are a class of plant hormones that control plant development in response to environmental conditions. In rice, mesocotyl elongation is regulated by SLs in the dark, while mesocotyls are longer i...Strigolactones （SLs） are a class of plant hormones that control plant development in response to environmental conditions. In rice, mesocotyl elongation is regulated by SLs in the dark, while mesocotyls are longer in SL deficient or insensitive mutants. SLs are perceived by DWARF14 （D14）, which is a member of a small gene family. In this study, we examined the function of another D14 family gene in rice, D14 LIKE （D14L）, focusing on mesocotyl growth. The mesocotyls of D14L RNAi lines are longer than those of WT in the dark. This phenotype is enhanced when the D14L RNAi lines are combined with the d14 mutation, suggesting that D14 and D14L work independently to inhibit mesocotyl elongation. This phenotype is alleviated by the exogenous supply of GR24, a synthetic SL, suggesting that D14L is not necessary for SL signaling. D14L mRNA is predominantly expressed in vascular bundles and crown root primordia. Our results suggest that D14L and D14 confer their effects via an SL independent pathway and an SL signaling pathway respectively.展开更多
文摘报道了中国希瓦氏菌D14 T 的Fe(Ⅲ)还原特性,研究了溶氧浓度、光照强度、温度、pH等条件对菌株Fe(Ⅲ)还原的影响。结果发现,随着培养基中Fe(Ⅲ)浓度的提高,菌株D14 T 的Fe(Ⅲ)还原速率相应降低;氧气和光照对Fe(Ⅲ)还原有一定的抑制作用;菌株还原Fe(Ⅲ)的最适反应温度为37℃;在反应起始pH6 0 - 10 0的条件下菌株可进行Fe(Ⅲ)还原。对不同形态Fe(Ⅲ)还原特性的研究结果表明,Fe(Ⅲ)的溶解度越高越有利于还原反应的进行。采用SDS和OGP这两种蛋白变性剂对Fe(Ⅲ)还原蛋白进行初步定位的结果表明,参与Fe(Ⅲ)还原的蛋白主要位于细胞可溶性外周蛋白。在同时含有偶氮染料和Fe(Ⅲ)的条件下,菌株D14 T 的偶氮染料脱色率和Fe(Ⅲ)
文摘AIM. To establish a kind of standardization of the clinical chemotherapeutic prototypes for unresectable hepatocellular carcinomas （HCC）. METHODS： 10-Hydroxycamptothecin （HCPT） was applied through transcatheter arterial embolization （TAE） to HCC patients who were categorized into three groups： （1） test group： treatment with HCPT twice （HCPT dl and 14） through TAE and portal venous embolization. （2） Control Ⅰ： treatment with anticancer drugs without HCPT. （3） Control Ⅱ： treatment with HCPT as a major component in anticancer drugs once （HCPT dl）. A set of comparisons between test groups and control Ⅰ and Ⅱ groups were performed before and after the treatment to study the effectiveness of each treatment, in terms of tumor volumes, dynamic variations in serum alpha-fetoprotein （AFP）, gamma-glutamyl transferase hepatoma-specific band （GGT-Ⅱ）, patient survival and adverse events. RESULTS： The general effectiveness rate of the test group reached 62.1% （72/116）, remarkably higher than that of control Ⅰ （32.1%, 40/124） and control Ⅱ （54.7%, 47/56）, （P〈0.01 and P〈0.05, respectively）. Especially, the reduction rate or disappearance of the portal vein tumor emboli was as high as 88.4% （61/69） in the test group, in contrast with 13.9% （10/72） in control Ⅰ and 35.9% （18/51） in control Ⅱ （P〈0.01 and P〈0.01, respectively）. After treatment, AFP decreased or turned to negative levels at 52.3% （34/65） in control Ⅰ, 67.3% （35/52） in control Ⅱ, and 96.8% （60/62） in the test group. Also GGT-Ⅱ declined or became negative at 37.8% （28/74） in control Ⅰ, 69.5% （57/82） in control Ⅱ, and 94.7% （89/94） in test group （P〈0.01 and P〈0.05, respectively）. CONCLUSION： We have designed a good protocol （test group） to treat HCC with excellent advantages of high efficiency, low cost, low toxicity and low adverse events and easy application. It could be recommended as one of the standardizations for HCC treatment in clinical practice.
基金supported by a grant from the Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN) of Japan to J.K
文摘Strigolactones （SLs） are a class of plant hormones that control plant development in response to environmental conditions. In rice, mesocotyl elongation is regulated by SLs in the dark, while mesocotyls are longer in SL deficient or insensitive mutants. SLs are perceived by DWARF14 （D14）, which is a member of a small gene family. In this study, we examined the function of another D14 family gene in rice, D14 LIKE （D14L）, focusing on mesocotyl growth. The mesocotyls of D14L RNAi lines are longer than those of WT in the dark. This phenotype is enhanced when the D14L RNAi lines are combined with the d14 mutation, suggesting that D14 and D14L work independently to inhibit mesocotyl elongation. This phenotype is alleviated by the exogenous supply of GR24, a synthetic SL, suggesting that D14L is not necessary for SL signaling. D14L mRNA is predominantly expressed in vascular bundles and crown root primordia. Our results suggest that D14L and D14 confer their effects via an SL independent pathway and an SL signaling pathway respectively.
