研究发现,Linux的内核模块具有良好的可控性而Linux的软中断具有极强的实时性。提出把两者有机地结合起来作为逻辑功能块(Logical Functional B lock,LFB)的实现机制,并用流标识号(PkfID)来描述LFB。实验表明采用这种方法实现的LFB能很...研究发现,Linux的内核模块具有良好的可控性而Linux的软中断具有极强的实时性。提出把两者有机地结合起来作为逻辑功能块(Logical Functional B lock,LFB)的实现机制,并用流标识号(PkfID)来描述LFB。实验表明采用这种方法实现的LFB能很好地满足ForCES需求(RFC 3654)。展开更多
目的介绍一种改良的Marsland法与Luxol fast blue(LFB)双重染色方法及其应用。方法用10%硝酸银溶液和0.2%Luxol fast blue溶液在一张石蜡切片上同时显示两种不同的神经组织成分。结果神经纤维呈黑色,神经元、轴索、树突呈黑色或紫黑色,...目的介绍一种改良的Marsland法与Luxol fast blue(LFB)双重染色方法及其应用。方法用10%硝酸银溶液和0.2%Luxol fast blue溶液在一张石蜡切片上同时显示两种不同的神经组织成分。结果神经纤维呈黑色,神经元、轴索、树突呈黑色或紫黑色,髓鞘呈鲜蓝色。结论改良的Marsland法与LFB双重染色方法,使神经纤维染成黑色,髓鞘呈鲜艳蓝色,两种颜色对比明显,背晃清晰。展开更多
为探究温度冲击引起的污泥膨胀机理,以生活污水为处理对象,采用SBR工艺分别运行温度骤降系统和温度梯度降低系统,利用Illumina MiSeq高通量测序技术分析温度变化过程中微生物群落整体变化,并对膨胀阶段优势丝状菌类型进行解析.结果表明...为探究温度冲击引起的污泥膨胀机理,以生活污水为处理对象,采用SBR工艺分别运行温度骤降系统和温度梯度降低系统,利用Illumina MiSeq高通量测序技术分析温度变化过程中微生物群落整体变化,并对膨胀阶段优势丝状菌类型进行解析.结果表明,温度骤降系统优势丝状菌为微丝菌(Microthrixparvicella),SVI值升高至291mL/g以上,温度梯度降低系统优势丝状菌为Eikelboom Type 0092型丝状菌,SVI值稳定维持在250mL/g,因此Eikelboom Type 0092型丝状菌适宜在温度冲击环境中生长繁殖.温度冲击方式不同导致菌群组成具有差异性,Proteobacteria相对丰度均值为39.3%,其占比在不同阶段变化较小.两个系统在污泥膨胀阶段Actinobacteria和Chloroflexi的相对丰度占比不同.各样本中与去除有机物相关微生物菌群丰度均值为13.6%,Nitrospira其相对丰度均值为2.48%,占NOB总含量80%以上.温度梯度降低系统发生的Eikelboom Type 0092型丝状菌型污泥微膨胀,其出水水质没有发生严重恶化,COD和NH_(4)^(+)-N的去除效果均高于温度骤降系统.展开更多
当今IP路由器的一个发展趋势是控制器(Con tro l E lem en t,CE)与转发器(Forw ard ing E lem en t,FE)的分离(Forw ard ing and Con tro l E lem en t Separation)。基于控制器和转发器分离的IP路由器比传统模式的IP路由器具有更高的...当今IP路由器的一个发展趋势是控制器(Con tro l E lem en t,CE)与转发器(Forw ard ing E lem en t,FE)的分离(Forw ard ing and Con tro l E lem en t Separation)。基于控制器和转发器分离的IP路由器比传统模式的IP路由器具有更高的可配置性和灵活性,更容易满足新业务对网络提出的新要求,从根本上解决IP路由器的Q oS问题。控制器在IP路由器中至关重要,他通过向转发器发送控制消息来控制整个路由器的动作,并且及时反映转发器的属性信息。对基于ForCES(Forw ard ing and Con tro l E lem en t Separation)结构的IP路由器中控制器进行了研究与实现,重点介绍网络件(N etw orkE lem en t,NE)信息描述的实现原理和逻辑功能块(Log ica l Function B lock,LFB)拓扑结构图形化显示的实现原理,最后给出了测试结果。展开更多
Here, we report a novel and universal methodology,termed ＂ntarctic thermolabile uracil-DNA-glycosylase （AUDG）-supplemented nucleic acid amplification techniques （NAAs） using a labeled-based nanoparticle lateral f...Here, we report a novel and universal methodology,termed ＂ntarctic thermolabile uracil-DNA-glycosylase （AUDG）-supplemented nucleic acid amplification techniques （NAAs） using a labeled-based nanoparticle lateral flow biosensor （LFB）＂ （AUDG-NAAs-LFB）, which merges enzymatic （AUDG） digestion of contaminant amplicons with different nucleic acid amplification techniques （NAAs）, and uses a lateral flow biosensor （LFB） for the rapid and visual confirmation of the presence of a target nucleic acid sequence. AUDG-NNAs-LFB is a one-pot, closedvessel assay, that can effectively eliminate false-positive signals arising from either carryover contaminants or the interaction between labeled primers. A new LFB was devised for detecting three targets （two amplicons generated from amplification of target sequences, and a chromatography control）, without the need for probe- hybridization or additional incubation steps. As a proof of concept, multiple cross displacement amplification （MCDA）, which is a specific, sensitive, and rapid isothermal amplification method, was selected as the model amplification technique to demonstrate the feasibility of AUDG-NAAs-LFB. As a result, we demonstrate the applicability of the AUDG-MCDA-LFB method for simultaneously detecting high-risk human papillomaviruses genotypes 16 and 18, which are the most and second-most prevalent strains of the virus reported in women worldwide. We also confirm the principle behind the AUDG-MCDA- LFB assay and validate its sensitivity, reproducibility, and specificity using serial dilutions of the type-specific plasmids, as well as clinical samples. This proof- of-concept method （AUDG-MCDA-LFB） can be easily reconfigured to detect various nudeic acid sequences by redesigning the specific MCDA primers.展开更多
文摘研究发现,Linux的内核模块具有良好的可控性而Linux的软中断具有极强的实时性。提出把两者有机地结合起来作为逻辑功能块(Logical Functional B lock,LFB)的实现机制,并用流标识号(PkfID)来描述LFB。实验表明采用这种方法实现的LFB能很好地满足ForCES需求(RFC 3654)。
文摘目的介绍一种改良的Marsland法与Luxol fast blue(LFB)双重染色方法及其应用。方法用10%硝酸银溶液和0.2%Luxol fast blue溶液在一张石蜡切片上同时显示两种不同的神经组织成分。结果神经纤维呈黑色,神经元、轴索、树突呈黑色或紫黑色,髓鞘呈鲜蓝色。结论改良的Marsland法与LFB双重染色方法,使神经纤维染成黑色,髓鞘呈鲜艳蓝色,两种颜色对比明显,背晃清晰。
文摘为探究温度冲击引起的污泥膨胀机理,以生活污水为处理对象,采用SBR工艺分别运行温度骤降系统和温度梯度降低系统,利用Illumina MiSeq高通量测序技术分析温度变化过程中微生物群落整体变化,并对膨胀阶段优势丝状菌类型进行解析.结果表明,温度骤降系统优势丝状菌为微丝菌(Microthrixparvicella),SVI值升高至291mL/g以上,温度梯度降低系统优势丝状菌为Eikelboom Type 0092型丝状菌,SVI值稳定维持在250mL/g,因此Eikelboom Type 0092型丝状菌适宜在温度冲击环境中生长繁殖.温度冲击方式不同导致菌群组成具有差异性,Proteobacteria相对丰度均值为39.3%,其占比在不同阶段变化较小.两个系统在污泥膨胀阶段Actinobacteria和Chloroflexi的相对丰度占比不同.各样本中与去除有机物相关微生物菌群丰度均值为13.6%,Nitrospira其相对丰度均值为2.48%,占NOB总含量80%以上.温度梯度降低系统发生的Eikelboom Type 0092型丝状菌型污泥微膨胀,其出水水质没有发生严重恶化,COD和NH_(4)^(+)-N的去除效果均高于温度骤降系统.
文摘当今IP路由器的一个发展趋势是控制器(Con tro l E lem en t,CE)与转发器(Forw ard ing E lem en t,FE)的分离(Forw ard ing and Con tro l E lem en t Separation)。基于控制器和转发器分离的IP路由器比传统模式的IP路由器具有更高的可配置性和灵活性,更容易满足新业务对网络提出的新要求,从根本上解决IP路由器的Q oS问题。控制器在IP路由器中至关重要,他通过向转发器发送控制消息来控制整个路由器的动作,并且及时反映转发器的属性信息。对基于ForCES(Forw ard ing and Con tro l E lem en t Separation)结构的IP路由器中控制器进行了研究与实现,重点介绍网络件(N etw orkE lem en t,NE)信息描述的实现原理和逻辑功能块(Log ica l Function B lock,LFB)拓扑结构图形化显示的实现原理,最后给出了测试结果。
文摘Here, we report a novel and universal methodology,termed ＂ntarctic thermolabile uracil-DNA-glycosylase （AUDG）-supplemented nucleic acid amplification techniques （NAAs） using a labeled-based nanoparticle lateral flow biosensor （LFB）＂ （AUDG-NAAs-LFB）, which merges enzymatic （AUDG） digestion of contaminant amplicons with different nucleic acid amplification techniques （NAAs）, and uses a lateral flow biosensor （LFB） for the rapid and visual confirmation of the presence of a target nucleic acid sequence. AUDG-NNAs-LFB is a one-pot, closedvessel assay, that can effectively eliminate false-positive signals arising from either carryover contaminants or the interaction between labeled primers. A new LFB was devised for detecting three targets （two amplicons generated from amplification of target sequences, and a chromatography control）, without the need for probe- hybridization or additional incubation steps. As a proof of concept, multiple cross displacement amplification （MCDA）, which is a specific, sensitive, and rapid isothermal amplification method, was selected as the model amplification technique to demonstrate the feasibility of AUDG-NAAs-LFB. As a result, we demonstrate the applicability of the AUDG-MCDA-LFB method for simultaneously detecting high-risk human papillomaviruses genotypes 16 and 18, which are the most and second-most prevalent strains of the virus reported in women worldwide. We also confirm the principle behind the AUDG-MCDA- LFB assay and validate its sensitivity, reproducibility, and specificity using serial dilutions of the type-specific plasmids, as well as clinical samples. This proof- of-concept method （AUDG-MCDA-LFB） can be easily reconfigured to detect various nudeic acid sequences by redesigning the specific MCDA primers.
