In order to study the effects of different extraction methods on the molar mass distribution and chain conformation of Fortunella margarita (Lour.) Swingle polysaccharides (FP), we used extraction by hot water (...In order to study the effects of different extraction methods on the molar mass distribution and chain conformation of Fortunella margarita (Lour.) Swingle polysaccharides (FP), we used extraction by hot water (WFP), ultrasonic-assisted treatment (UFP), microwave-assisted treatment (MFP) and ultrasonic/microwave-assisted treatment (UMFP), and then Fourier transform infrared (FT-IR) spectroscopy as well as 1H and 13C nuclear magnetic resonance (NMR) spectroscopy to characterize the structural properties of FP extracted. The molar weight (Mw), polydispersity index (Mw/Mn), root-mean-square (RMS) turning radius (Rg), molar mass distribution and chain conformation of FP were studied systematically using size-exclusion chromatography (SEC), multi-angle laser light-scattering (MALLS) and refractive index (RI). WFP, UFP, MFP and UMFP are all typical carbohydrates according to 1H NMR, laC NMR and FT-IR measurements. The type of glycosidic linkage is mainly a fl-glycosidic bond with a small amount of a-glycosidic bond. The results obtained by the SEC-MALLS-RI system showed the molar masses of WFP and UMFP were distributed mainly in the range of 5.0x10^6-1.0x10^7 g-mo1-1 and they accounted for 57.80% and 56.84% of total FP, respectively. The molar masses of UFP and MFP were distributed mainly in the 1.0x10^6-5.0x10^6 g.mol-1 range, which accounted for 38.24% and 52.39% of FP, respectively. WFP and UMFP in water were uniform spherical polymers; UFP and MFP were typical highly branched polymers and the degree of branching for MFP was higher compared to UFP. These results indicated the ultrasonic- and microwave-assisted extraction methods caused a significant decrease of the molar mass of FP but the ultrasonic/microwave synergistic extraction method had no effect.展开更多
Fortunella margarita(Lour.) Swingle, commonly known as kumquat, is the smallest citrus fruit. It thrives in southeastern China and is widely cultivated and consumed in the world due to its multiple health benefits. ...Fortunella margarita(Lour.) Swingle, commonly known as kumquat, is the smallest citrus fruit. It thrives in southeastern China and is widely cultivated and consumed in the world due to its multiple health benefits. It has been used as an important herbal medicine in traditional Chinese medicine and also as one of the most popular fruits. There are various kinds of bioactive compounds in F. margarita, such as polysaccharides, limonoids, essential oils, flavonoids, phenolic acids, vitamins, dietary fiber, etc. In addition, many studies have reported that these bioactive compounds can be used as antioxidant, antimicrobial, hypolipidemic, drosophila lure components in functional foods, pharmaceuticals and daily chemical products due to their biological activities. This review focuses on the structural features and biological activities of polysaccharides, limonoids, essential oils and flavonoids and other bioactive substances from F. margarita and their potential applications in food, daily chemical and pharmaceutical industries.展开更多
[目的]低温是导致香蕉减产的主要自然灾害之一。基于转录组与蛋白质组关联分析参与香蕉抗寒的相关基因、蛋白及信号、代谢通路调控网络,探讨香蕉抗寒的分子机制。[方法]以巴西蕉为试材,在7℃低温下处理1 d(Cold1)和3 d(Cold3),以28℃培...[目的]低温是导致香蕉减产的主要自然灾害之一。基于转录组与蛋白质组关联分析参与香蕉抗寒的相关基因、蛋白及信号、代谢通路调控网络,探讨香蕉抗寒的分子机制。[方法]以巴西蕉为试材,在7℃低温下处理1 d(Cold1)和3 d(Cold3),以28℃培养的巴西蕉为对照(CK),基于笔者课题组前期获得的蛋白质组数据,利用转录组测序技术检测巴西蕉在低温胁迫下基因调控网络的变化,同时与蛋白质组学数据进行关联分析,共同解析巴西蕉响应低温胁迫的分子机制。[结果]转录组分析结果显示,Cold1 vs CK、Cold3 vs CK和Cold1 vs Cold3三个对比组分别鉴定出11 370、15 460和9 619个差异表达基因,对这些基因的KEGG富集分析发现,差异表达基因在光合作用信号、谷胱甘肽代谢、α-亚麻酸代谢途径和苯丙素生物合成等多个低温胁迫关键信号代谢通路中富集。对部分差异表达基因进行实时荧光定量(qRT-PCR)分析,其中DREB、MAPK和MYB等低温调控关键基因的表达量在低温处理后显著上升,所选20个基因的表达变化趋势与RNA-seq基本相符,证实了RNA-seq的准确性。转录组与蛋白质组关联分析结果显示,共鉴定到6 211个与转录本相对应的蛋白,转录组与蛋白质呈现正相关关系,共有105个转录本及其对应的蛋白表达量共同上调,有218个转录本及其对应的蛋白表达量共同下调。GO富集分析显示,差异表达基因和蛋白在光响应、叶绿体、氧化还原酶活性等功能大量富集。此外,对差异表达基因和蛋白KEGG通路的关联分析发现,低温处理抑制了苯丙素生物合成和光合作用信号途径相关基因及蛋白的表达,促进了α-亚麻酸代谢和谷胱甘肽途径的表达。[结论]运用转录组结合蛋白质组学技术绘制了基因和蛋白质水平上的香蕉抗寒调控网络,并发现香蕉响应低温的信号通路主要涉及光合作用信号、谷胱甘肽代谢、α-亚麻酸代谢途径和苯丙素生物合成等途径。展开更多
基金Supported by Science and Technology Plan Major Project of Fujian Province(2013Y0003)Agricultural Products(fruits and vegetables) Processing Engineering Technology Research Center Funding Projects of Fujian Province(2009N2002)+1 种基金Scientific and Technological Innovation Team Support Plan of Institution of Higher Learning in Fujian Province([2012]03)Scientific and Technological Innovation Team Support Plan of Fujian Agriculture and Forestry University(cxtd12009)
文摘In order to study the effects of different extraction methods on the molar mass distribution and chain conformation of Fortunella margarita (Lour.) Swingle polysaccharides (FP), we used extraction by hot water (WFP), ultrasonic-assisted treatment (UFP), microwave-assisted treatment (MFP) and ultrasonic/microwave-assisted treatment (UMFP), and then Fourier transform infrared (FT-IR) spectroscopy as well as 1H and 13C nuclear magnetic resonance (NMR) spectroscopy to characterize the structural properties of FP extracted. The molar weight (Mw), polydispersity index (Mw/Mn), root-mean-square (RMS) turning radius (Rg), molar mass distribution and chain conformation of FP were studied systematically using size-exclusion chromatography (SEC), multi-angle laser light-scattering (MALLS) and refractive index (RI). WFP, UFP, MFP and UMFP are all typical carbohydrates according to 1H NMR, laC NMR and FT-IR measurements. The type of glycosidic linkage is mainly a fl-glycosidic bond with a small amount of a-glycosidic bond. The results obtained by the SEC-MALLS-RI system showed the molar masses of WFP and UMFP were distributed mainly in the range of 5.0x10^6-1.0x10^7 g-mo1-1 and they accounted for 57.80% and 56.84% of total FP, respectively. The molar masses of UFP and MFP were distributed mainly in the 1.0x10^6-5.0x10^6 g.mol-1 range, which accounted for 38.24% and 52.39% of FP, respectively. WFP and UMFP in water were uniform spherical polymers; UFP and MFP were typical highly branched polymers and the degree of branching for MFP was higher compared to UFP. These results indicated the ultrasonic- and microwave-assisted extraction methods caused a significant decrease of the molar mass of FP but the ultrasonic/microwave synergistic extraction method had no effect.
基金Supported by the Natural Science Foundation of Fujian Province(2016J05068)High Level University Construction Projects of Fujian Agriculture and Forestry University(612014042)+2 种基金Science and Technology Development Foundation Project of Fujian Agriculture and Forestry University(KF2015101)Leading Talents Support Program of Science and Technology Innovation in Fujian Province(KRC16002A)Excellent Talents Support Program of Colleges and Universities in Fujian Province(JA14094)
文摘Fortunella margarita(Lour.) Swingle, commonly known as kumquat, is the smallest citrus fruit. It thrives in southeastern China and is widely cultivated and consumed in the world due to its multiple health benefits. It has been used as an important herbal medicine in traditional Chinese medicine and also as one of the most popular fruits. There are various kinds of bioactive compounds in F. margarita, such as polysaccharides, limonoids, essential oils, flavonoids, phenolic acids, vitamins, dietary fiber, etc. In addition, many studies have reported that these bioactive compounds can be used as antioxidant, antimicrobial, hypolipidemic, drosophila lure components in functional foods, pharmaceuticals and daily chemical products due to their biological activities. This review focuses on the structural features and biological activities of polysaccharides, limonoids, essential oils and flavonoids and other bioactive substances from F. margarita and their potential applications in food, daily chemical and pharmaceutical industries.
文摘[目的]低温是导致香蕉减产的主要自然灾害之一。基于转录组与蛋白质组关联分析参与香蕉抗寒的相关基因、蛋白及信号、代谢通路调控网络,探讨香蕉抗寒的分子机制。[方法]以巴西蕉为试材,在7℃低温下处理1 d(Cold1)和3 d(Cold3),以28℃培养的巴西蕉为对照(CK),基于笔者课题组前期获得的蛋白质组数据,利用转录组测序技术检测巴西蕉在低温胁迫下基因调控网络的变化,同时与蛋白质组学数据进行关联分析,共同解析巴西蕉响应低温胁迫的分子机制。[结果]转录组分析结果显示,Cold1 vs CK、Cold3 vs CK和Cold1 vs Cold3三个对比组分别鉴定出11 370、15 460和9 619个差异表达基因,对这些基因的KEGG富集分析发现,差异表达基因在光合作用信号、谷胱甘肽代谢、α-亚麻酸代谢途径和苯丙素生物合成等多个低温胁迫关键信号代谢通路中富集。对部分差异表达基因进行实时荧光定量(qRT-PCR)分析,其中DREB、MAPK和MYB等低温调控关键基因的表达量在低温处理后显著上升,所选20个基因的表达变化趋势与RNA-seq基本相符,证实了RNA-seq的准确性。转录组与蛋白质组关联分析结果显示,共鉴定到6 211个与转录本相对应的蛋白,转录组与蛋白质呈现正相关关系,共有105个转录本及其对应的蛋白表达量共同上调,有218个转录本及其对应的蛋白表达量共同下调。GO富集分析显示,差异表达基因和蛋白在光响应、叶绿体、氧化还原酶活性等功能大量富集。此外,对差异表达基因和蛋白KEGG通路的关联分析发现,低温处理抑制了苯丙素生物合成和光合作用信号途径相关基因及蛋白的表达,促进了α-亚麻酸代谢和谷胱甘肽途径的表达。[结论]运用转录组结合蛋白质组学技术绘制了基因和蛋白质水平上的香蕉抗寒调控网络,并发现香蕉响应低温的信号通路主要涉及光合作用信号、谷胱甘肽代谢、α-亚麻酸代谢途径和苯丙素生物合成等途径。