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山羊支原体山羊肺炎亚种MCCG-0521乳酸脱氢酶基因的克隆表达及其编码氨基酸的生物学分析
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作者 刘威 蒋鹏程 +7 位作者 杨克礼 袁芳艳 周丹娜 刘泽文 高婷 郭锐 孙裴 田永祥 《动物医学进展》 北大核心 2023年第2期35-40,共6页
旨在获得山羊支原体山羊肺炎亚种MCCG-0521乳酸脱氢酶(L-lactate dehydrogenase,LDH)的蛋白表达产物,分析其编码氨基酸的生物学特性,为进一步研究LDH蛋白的功能奠定基础。试验完成了LDH基因的克隆和密码子改造,成功将LDH基因中473、761... 旨在获得山羊支原体山羊肺炎亚种MCCG-0521乳酸脱氢酶(L-lactate dehydrogenase,LDH)的蛋白表达产物,分析其编码氨基酸的生物学特性,为进一步研究LDH蛋白的功能奠定基础。试验完成了LDH基因的克隆和密码子改造,成功将LDH基因中473、761、779 nt处的A突变为G,使改造后的LDH基因能在大肠埃希氏菌Rosetta中正确表达。SDS-PAGE电泳结果显示,IPTG诱导目的蛋白表达后获得一条大小约34 ku的蛋白条带,Western blot结果表明获得的原核表达产物可与抗6×His tag的单克隆抗体发生特异性反应,研究采用DNAStar Protean模块分析了LDH的二级结构、蛋白骨架柔性区域和可能的B细胞抗原优势表位,为进一步研究其功能奠定了基础。 展开更多
关键词 山羊支原体山羊肺炎亚种 mccg-0521 乳酸脱氢酶 密码子改造 二级结构
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Embedding Economic Excellence: A Transformational Definition of "Corporate Governance" for Malaysia
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作者 Zulkiffiee Mohamed Garry James Clayton Mohd Yaziz Mohd Isa 《Journal of Modern Accounting and Auditing》 2015年第2期124-129,共6页
The Malaysian government has carefully crafted policies to ensure that the nation achieves its aspirational goal to become a developed nation by 2020. Crucial for which is the success of the Securities Commission's ... The Malaysian government has carefully crafted policies to ensure that the nation achieves its aspirational goal to become a developed nation by 2020. Crucial for which is the success of the Securities Commission's "Corporate Governance Blueprint 2011" and "Capital Market Master Plan 2" designed to provide a business environment which attracts investment and talent. Somewhat overlooked in these otherwise robust policy shifts has been a critical critique of the definition, nature, and purpose of corporate governance itself. Recognizing this oversight, Tan Sri Muhyiddin Yassin, Malaysia's Deputy Prime Minister, has directed that the definition of corporate governance be rejuvenated for the 21st century. This paper looks to answer that challenge by recommending the following transformational definition for corporate governance: "the proactive implementation of accountable ethical processes, procedures, and policies that inspires innovative aspirational economic activity to produce sustainable wealth and prosperity for shareholders, stakeholders, and society". 展开更多
关键词 corporate governance Malaysian Code of Corporate Governance (mccg) SUSTAINABILITY MALAYSIA
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