以小麦光温敏核雄性不育系BS20×Fu3双单倍体（DH）群体的289个系为材料,从1112对SSR和EST-SSR引物中筛选出多态性引物243对,利用其中128个SSR和6个EST-SSR标记构建遗传连锁图谱,该图谱覆盖长度为2749.2 c M,分布在小麦的19个连锁...以小麦光温敏核雄性不育系BS20×Fu3双单倍体（DH）群体的289个系为材料,从1112对SSR和EST-SSR引物中筛选出多态性引物243对,利用其中128个SSR和6个EST-SSR标记构建遗传连锁图谱,该图谱覆盖长度为2749.2 c M,分布在小麦的19个连锁群（除4D、6A）,不同连锁群标记数为2-15个,长度在15.3-244.4 c M之间,平均长度为144.7 c M,标记之间平均遗传距离为17.4 c M。同时构建3个DNA池（包括恢复池、北京不育池和阜阳不育池）,用分离群体分组分析法（BSA）对育性进行分析,筛选出的多态性引物为Wmc264、Wmc73、Xgwm350,分布在3A、5B、2A/7D染色体上。同时用混合线性复合区间作图法（MCIM）对育性进行QTL分析,当F〉7.5时,检测到6个主效QTL,用复合区间作图法（CIM）对育性进行QTL分析,当LOD值〉2.5时,共检测到13个主效QTL,两种方法检测到一致的QTL有3个,分别为1BL的Wmc365-cfa2129、2BS的Wmc602-Xgwm148和3AL的Wmc264a-cfa2262区间的QTL。综合BSA和QTL的结果,位于1BL、2BS和3AL上的小麦光温敏不育基因是真实的。展开更多
The adaptability of soybean to be grown at a wide range of latitudes is attributed to natural variation in the major genes and quantitative trait loci （QTLs） that control flowering time and maturity. Thus, the ident...The adaptability of soybean to be grown at a wide range of latitudes is attributed to natural variation in the major genes and quantitative trait loci （QTLs） that control flowering time and maturity. Thus, the identification of genes controlling flowering time and maturity and the understanding of their molecular basis are critical for improving soybean productivity. However, due to the great effect of the major maturity gene E1 on flowering time, it is difficult to detect other small-effect QTLs. In this study, aiming to reduce the effect of the QTL, associated with the E1 gene, on the detection of other QTLs, we divided a population of 96 recombinant inbred lines （RILs） into two sub-populations： one with the E1 allele and another with the elns allele. Compared with the results of using all 96 recombinant inbred lines, additional QTLs for flowering time were identified in the sub-populations, two （qFT-B1 and qFT-H） in RILs with the E1 allele and one （qFT-J-2） in the RILs with the elnl allele, respectively. The three QTLs, qFT-B1, qFT-H and qFT-J-2 were true QTLs and played an important role in the regulation of growth period. Our data provides valuable information for the genetic mapping and gene cloning of traits controlling flowering time and maturity and will help a better understanding of the mechanism of photoperiod-regulated flowering and molecular breeding in soybean.展开更多
Most often a genetic linkage map is prepared using populations obtained from two highly diverse genotypes. However, the markers from such a map may not be useful in a breeding program as these markers may not
文摘以小麦光温敏核雄性不育系BS20×Fu3双单倍体（DH）群体的289个系为材料,从1112对SSR和EST-SSR引物中筛选出多态性引物243对,利用其中128个SSR和6个EST-SSR标记构建遗传连锁图谱,该图谱覆盖长度为2749.2 c M,分布在小麦的19个连锁群（除4D、6A）,不同连锁群标记数为2-15个,长度在15.3-244.4 c M之间,平均长度为144.7 c M,标记之间平均遗传距离为17.4 c M。同时构建3个DNA池（包括恢复池、北京不育池和阜阳不育池）,用分离群体分组分析法（BSA）对育性进行分析,筛选出的多态性引物为Wmc264、Wmc73、Xgwm350,分布在3A、5B、2A/7D染色体上。同时用混合线性复合区间作图法（MCIM）对育性进行QTL分析,当F〉7.5时,检测到6个主效QTL,用复合区间作图法（CIM）对育性进行QTL分析,当LOD值〉2.5时,共检测到13个主效QTL,两种方法检测到一致的QTL有3个,分别为1BL的Wmc365-cfa2129、2BS的Wmc602-Xgwm148和3AL的Wmc264a-cfa2262区间的QTL。综合BSA和QTL的结果,位于1BL、2BS和3AL上的小麦光温敏不育基因是真实的。
基金partially supported by the National Natural Science Foundation of China (31430065, 31571686, 31201222 and 31371643)the Open Foundation of the Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences+5 种基金the “Hundred Talents” Program of the Chinese Academy of Sciencesthe Strategic Action Plan for Science and Technology Innovation of the Chinese Academy of Sciences (XDA08030108)the Natural Science Foundation of Heilongjiang Province, China (ZD201001, JC201313)the Research and Development of Applied Technology Project, Harbin, China (2014RFQYJ055)the Scientific Research Foundation for Returned Chinese Scholars of Heilongjiang Province, China (LC201417)the Science Foundation for Creative Research Talents of Harbin Science and Technology Bureau, China (2014RFQYJ046)
文摘The adaptability of soybean to be grown at a wide range of latitudes is attributed to natural variation in the major genes and quantitative trait loci （QTLs） that control flowering time and maturity. Thus, the identification of genes controlling flowering time and maturity and the understanding of their molecular basis are critical for improving soybean productivity. However, due to the great effect of the major maturity gene E1 on flowering time, it is difficult to detect other small-effect QTLs. In this study, aiming to reduce the effect of the QTL, associated with the E1 gene, on the detection of other QTLs, we divided a population of 96 recombinant inbred lines （RILs） into two sub-populations： one with the E1 allele and another with the elns allele. Compared with the results of using all 96 recombinant inbred lines, additional QTLs for flowering time were identified in the sub-populations, two （qFT-B1 and qFT-H） in RILs with the E1 allele and one （qFT-J-2） in the RILs with the elnl allele, respectively. The three QTLs, qFT-B1, qFT-H and qFT-J-2 were true QTLs and played an important role in the regulation of growth period. Our data provides valuable information for the genetic mapping and gene cloning of traits controlling flowering time and maturity and will help a better understanding of the mechanism of photoperiod-regulated flowering and molecular breeding in soybean.
文摘Most often a genetic linkage map is prepared using populations obtained from two highly diverse genotypes. However, the markers from such a map may not be useful in a breeding program as these markers may not
