The northem pig-tailed macaque （NPM, Macaca leonina） has become a widely used animal model in biomedical research. In this study, we measured serum immunoglobulin IgG, IgM, IgA, complement C3, C4 and CRP levels in 3...The northem pig-tailed macaque （NPM, Macaca leonina） has become a widely used animal model in biomedical research. In this study, we measured serum immunoglobulin IgG, IgM, IgA, complement C3, C4 and CRP levels in 3-11 year old captive northem pig-tailed macaques using HITACHI 7600-20 automated chemistry analyzer in order to determine the influences of age and gender on these items. The results showed that serum IgA, IgM, C3 and C4 levels were not correlated with age （P〉0.05）, while serum IgG levels increased progressively with age （r=0.202; P=0.045）. Serum IgG, IgA, IgM and C3 levels were higher in females than in males （P〈0.05）. Moreover, serum C3 concentration was both positively and strongly correlated with that of C4 （r=0.700; P〈0.0001）. This study provides basic serum immunoglobulin and complement data of captive northem pig-tailed macaques, which may prove useful for future breeding efforts and biomedical research.展开更多
The northern pig-tailed macaque （Macaca leonina） has been identified as an independent species of Old World monkey, and we previously found that PBMCs from M. leonina were susceptible to human immunodeficiency virus...The northern pig-tailed macaque （Macaca leonina） has been identified as an independent species of Old World monkey, and we previously found that PBMCs from M. leonina were susceptible to human immunodeficiency virus type 1 （HIV-1）, which may be due to the absence of a TRIM5 protein restricting HIV-1 replication. Here we investigated the infection potentials of six laboratory adapted HIV-1 strains and three primary HIV-1 isolates in PBMCs from M. leonina. The results indicate that these strains are characterized by various but low replication levels, and among which, HIV-INL4-3 shows the highest replication ability. Based on the abundant evidence of species-specific interactions between restriction factors APOBEC3 and HIV/SIV-derived Vif protein, we subsequently examined the replication potentials of v/f-substituted HIV-1 （HSIV） in M. leonina PBMCs. Notably, HSIV-vifmac and stHIV-lsv chimeras, two HIV-1Ni.4-3-derived viruses encoding the viral infectivity factor （Vif） protein from SIVmac239, replicated robustly in cells from M. leonina, which suggests that HSIV could effectively antagonize the antiviral activity of APOBEC3 proteins expressed in cells of M. leonina. Therefore, our data demonstrate that M. leonina has the potential to be developed into a promising animal model for human AIDS.展开更多
种间相互作用,包括竞争、捕食和寄生等,对动物种群动态的调控具有重要影响(Akçakaya et al.,2019;Mittelbach and McGill,2019)。当具有相似生态习性的物种同域分布,并且空间和食物等必要生存资源有限时,就可能发生种间竞争,进而...种间相互作用,包括竞争、捕食和寄生等,对动物种群动态的调控具有重要影响(Akçakaya et al.,2019;Mittelbach and McGill,2019)。当具有相似生态习性的物种同域分布,并且空间和食物等必要生存资源有限时,就可能发生种间竞争,进而导致其生态位的分化。种间竞争包括两种主要形式,即利用型竞争(exploitation competition)和干涉型竞争(interference competition)(Dhondt,2012;Chen et al.,2020)。同一资源对两种动物都非常重要,但两者竞争过程中不发生直接冲突,表现为利用型竞争;一种动物借助于行为干扰另一种动物使其得不到资源,且发生直接冲突,则为干涉型竞争(Kro‐nfeld-Schor and Dayan,2003;Dhondt,2012)。这些竞争关系可能影响物种种群的稳定,或者使物种对资源的利用发生转变以避免竞争,从而实现长期共存(Mittelbach and McGill,2019)。展开更多
基金Foundation items： This work was supported by the National Natural Science Foundation of China （81172876, U0832601, 81273251, U1202228） the National Special Science Research Program of China （2012CBA01305） the National Science and Technology Major Project （2013ZX10001-002, 2012ZX10001-007） and the Knowledge Innovation Program of CAS （KSCX2-EW-R-13）.
文摘The northem pig-tailed macaque （NPM, Macaca leonina） has become a widely used animal model in biomedical research. In this study, we measured serum immunoglobulin IgG, IgM, IgA, complement C3, C4 and CRP levels in 3-11 year old captive northem pig-tailed macaques using HITACHI 7600-20 automated chemistry analyzer in order to determine the influences of age and gender on these items. The results showed that serum IgA, IgM, C3 and C4 levels were not correlated with age （P〉0.05）, while serum IgG levels increased progressively with age （r=0.202; P=0.045）. Serum IgG, IgA, IgM and C3 levels were higher in females than in males （P〈0.05）. Moreover, serum C3 concentration was both positively and strongly correlated with that of C4 （r=0.700; P〈0.0001）. This study provides basic serum immunoglobulin and complement data of captive northem pig-tailed macaques, which may prove useful for future breeding efforts and biomedical research.
基金Foundation items： This work was supported by the National Basic Research Program （2012CBA01305） the National Natural Science Foundation of China （81172876, U0832601, 81273251 and U 1202228） the Knowledge Innovation Program of CAS （KSCX2-EW-R-13, Y206A- 71181）, and the Key Scientific and Technological Program of China （2012ZX10001-007, 2013ZX10001-002）. Acknowledgements： We thank Prof. Guang-Xia GAO （Institute of Biophysics, Chinese Academy of Sciences） for kindly providing HSIV proviral plasmids.We also thank Long-Bao LV, Gui LI and Dong- Ti HUANG of Kunming Primate Research Center for their assistance in obtaining blood samples from northem pig-tailed macaques （M. leonina） and Chinese rhesus macaques.
文摘The northern pig-tailed macaque （Macaca leonina） has been identified as an independent species of Old World monkey, and we previously found that PBMCs from M. leonina were susceptible to human immunodeficiency virus type 1 （HIV-1）, which may be due to the absence of a TRIM5 protein restricting HIV-1 replication. Here we investigated the infection potentials of six laboratory adapted HIV-1 strains and three primary HIV-1 isolates in PBMCs from M. leonina. The results indicate that these strains are characterized by various but low replication levels, and among which, HIV-INL4-3 shows the highest replication ability. Based on the abundant evidence of species-specific interactions between restriction factors APOBEC3 and HIV/SIV-derived Vif protein, we subsequently examined the replication potentials of v/f-substituted HIV-1 （HSIV） in M. leonina PBMCs. Notably, HSIV-vifmac and stHIV-lsv chimeras, two HIV-1Ni.4-3-derived viruses encoding the viral infectivity factor （Vif） protein from SIVmac239, replicated robustly in cells from M. leonina, which suggests that HSIV could effectively antagonize the antiviral activity of APOBEC3 proteins expressed in cells of M. leonina. Therefore, our data demonstrate that M. leonina has the potential to be developed into a promising animal model for human AIDS.
文摘目的本研究旨在建立一种实时荧光定量PCR方法,用于检测猕猴三磷酸腺苷结合盒转运蛋白G2(adenosine triphosphate-binding cassette transporter protein G2,ABCG2)mRNA的基因转录水平。方法使用NCBI上GenBank数据库猕猴(Macaca mulatta)的ABCG2核苷酸序列号NM_001032919.1及内参GAPDH核苷酸序列号NM_001195426.1,借助Primer premier 5.0软件设计PCR引物。提取猕猴新鲜肾组织的总RNA,并反转录合成cDNA。接着,利用PCR引物进行实时荧光定量PCR扩增,并根据反应体系中荧光的变化情况定量分析ABCG2的mRNA相对表达水平。结果PCR产物测序结果显示,扩增的ABCG2和GAPDH核苷酸序列与NCBI上猕猴的序列同源性分别为90.91%和91.14%。ABCG2和GAPDH的扩增效率均达到80%~120%,实时荧光定量PCR标准曲线的熔解曲线为单峰,R2接近1。结论本研究建立的检测猕猴ABCG2 mRNA实时荧光定量检测方法,为研究高尿酸血症的发病机制以及新药开发奠定基础。
文摘种间相互作用,包括竞争、捕食和寄生等,对动物种群动态的调控具有重要影响(Akçakaya et al.,2019;Mittelbach and McGill,2019)。当具有相似生态习性的物种同域分布,并且空间和食物等必要生存资源有限时,就可能发生种间竞争,进而导致其生态位的分化。种间竞争包括两种主要形式,即利用型竞争(exploitation competition)和干涉型竞争(interference competition)(Dhondt,2012;Chen et al.,2020)。同一资源对两种动物都非常重要,但两者竞争过程中不发生直接冲突,表现为利用型竞争;一种动物借助于行为干扰另一种动物使其得不到资源,且发生直接冲突,则为干涉型竞争(Kro‐nfeld-Schor and Dayan,2003;Dhondt,2012)。这些竞争关系可能影响物种种群的稳定,或者使物种对资源的利用发生转变以避免竞争,从而实现长期共存(Mittelbach and McGill,2019)。
