An understanding of the knowledge creation and diffusion process in the organizational context is extremely relevant. Because from this understanding, organizations can restructure processes, reorient teams and implem...An understanding of the knowledge creation and diffusion process in the organizational context is extremely relevant. Because from this understanding, organizations can restructure processes, reorient teams and implement methodologies to assist in the construction of an evolutionary process of knowledge creation and diffusion aimed at sustainable growth and innovation. The theory of complex social networks has been applied in several fields to help understand organizational cognitive processes. However, these approaches still insipiently consider the analysis of the nestedness and modularity of the studied networks. In this article, we presented an approach that sought to identify patterns of nestedness and modularity in networks of affiliation of people in projects in the organizational context. The study sought to identify these patterns in affiliation networks in a public organization providing information technology services in the period from 2006 to 2013. The detection of these patterns was performed using the NODF (Nestedness metric based on Overlap and Decreasing Fill) algorithm described by <a href="#ref1">[1]</a>. The nestedness and modularity metrics can influence patterns of knowledge creation and diffusion in formal and informal networks constituted for the execution of projects in organizations. This study showed that the network structures of the organization during the study period presented a high degree of nestedness, and it was possible to identify combined structures of nestedness and modularity.展开更多
We compare and contrast asymmetry and nestedness, two concepts used in the characterisation of the specialist-generalist balance in bipartite ecological interaction networks. Our analysis is relevant to mutualistic ne...We compare and contrast asymmetry and nestedness, two concepts used in the characterisation of the specialist-generalist balance in bipartite ecological interaction networks. Our analysis is relevant to mutualistic networks such as those consisting of flowering plants and pollinators, or fruiting plants and frugivores, or antagonistic networks such as those consisting of plants and herbivores, in an ecological community. We shall refer to the two sets of species in the bipartite network as plants and animals, the usual but not the only ecological situation. By asymmetry we mean either connectivity asymmetry or dependence asymmetry, which are essentially equivalent. Asymmetry expresses two attributes: generalists interact preferentially with specialists, and specialists avoid interacting with each other. Nested patterns, in principle, should express these same two features and one more: the presence of a core of interactions among generalists. We compute the full set of perfectly nested patterns that are possible in an L × L matrix with N interactions representing an ecological network of L plants and L animals, and point out that the number of nested arrangements grows exponentially with N. In addition, we analyse asymmetry for the full set of perfectly nested patterns, and identify extremes of asymmetry inside the universe of nested patterns. The minimal asymmetry is marked by a modular core of interactions between species that are neither specialists nor generalists. On the other hand, the case of maximal asymmetry is formed by a set of few generalists and many specialists with equal connectivity. The stereotypic case of nestedness with a core of interactions among generalists has intermediate asymmetry.展开更多
城市中的园林绿地呈现斑块状分布,其栖息地特征与岛屿栖息地相似。2008年11月至2009年10月,对上海市闵行区内的7块城市绿地进行调查,记录雀形目鸟类的分布情况,并运用Nestedness temperature calculator软件,检验其群落结构是否符合嵌...城市中的园林绿地呈现斑块状分布,其栖息地特征与岛屿栖息地相似。2008年11月至2009年10月,对上海市闵行区内的7块城市绿地进行调查,记录雀形目鸟类的分布情况,并运用Nestedness temperature calculator软件,检验其群落结构是否符合嵌套结构。运用Arc GIS软件分析该地区的卫星图片,收集7块样地的面积、绿地盖度、水源距离和人为干扰程度等数据,结合实地调查所得到的数据,分析这一嵌套结构的形成原因和影响因素。调查结果显示:上海市闵行区城市绿地中的雀形目鸟类分布是显著的嵌套结构,矩阵系统温度是21.78℃,填充度41.3%。7块样地中出现的物种数从14种到38种不等。其中,白鹡鸰(Motacilla alba)等11种鸟在7块样地中都有分布,黑卷尾(Dicrurusmacrocerus)等9种鸟类只在一个样地中出现过。园林面积、绿地面积和水源情况都对其嵌套结构有显著影响。但与真正岛屿上存在的群落分布嵌套结构不同,人为干扰程度对这一结构也有非常明显的影响。上述结果表明,影响上海闵行区园林鸟类群落嵌套结构的主要原因是栖息地结构和人为干扰程度。因此,建议在规划和建设城市公园和绿地时,应该偏重于面积较大,植被盖度和丰富度高,结构合理的园林,并尽量减少人为干扰对鸟类栖息地的影响。展开更多
Objective： Successful treatment of breast cancer greatly depends on the early detection of its metastasis, therefore a sensitive and specific biomarker for detecting dissemination of the cancer cells will help to ach...Objective： Successful treatment of breast cancer greatly depends on the early detection of its metastasis, therefore a sensitive and specific biomarker for detecting dissemination of the cancer cells will help to achieve this goal. This study was to evaluate the prognostic significance of human small breast epithelial mucin （hSBEM） and CD44V6 in breast cancer. Methods： The expressions of hSBEM mRNA and CD44V6 mRNA were detected with nested reverse transcription polymerase chain reaction （nested RT-PCR） in 67 samples of breast cancer and adjacent normal breast tissue, 16 samples of breast benign lesions tissue, and 67 specimens of peripheral blood from patients with breast cancer, 16 specimens of benign breast lesions, 20 specimens of healthy volunteers, and 25 （each five cases） other carcinomas tissue samples, including those of gastric carcinoma, colorectal carcinoma, esophageal carcinoma, lung carcinoma, and ovary carcinoma, were analyzed for hSBEM mRNA expression by nested RT-PCR. Results： hSBEM mRNA expression was observed in 62/67 （92.54%） of breast cancer, 14/16 （87.50%） of breast benign lesions and 59/67 （88.05%） of normal breast tissue, with no significant differences between them （P 〉 0.05）. None of the samples from other cancer tissues were positive. In peripheral blood the expression of hSBEM mRNA was detected in 34/67 （50.75%） from patients with breast cancer, with significant increasing （P 〈 0.05） in the cases of metastatic disease （stage Ⅳ） and those with lymph node metastasis compared with localized disease （stage Ⅰ, Ⅱ and Ⅲ） and without lymph node metastasis, but its expression was not found in peripheral blood of patients with benign breast lesions or healthy volunteers. Although CD44V6 mRNA was significantly higher in breast cancer than in benign breast lesions tissue and normal breast tissue, its expression in peripheral blood show no significant difference （P 〉 0.05） in the patients with breast cancer （82.09%）, benign breast lesion （75.00%）, or healthy volunteers （70.00%）. The expressions of hSBEM mRNA and CD44V6 mRNA had no correlation with the age of the patients, size of primary tumor, histological type and estrogen or progestin receptor status （P 〉 0.05）. Conclusion： hSBEM mRNA, as assessed by nested RT-PCR, shows a mammary-specific and mammary-sensitive expression, and is a sensitive indicator of hematogeneous spread of breast cancer cell, while CD44V6 shows low sensitivity and specificity in detecting dissemination of breast cancer cell in peripheral blood, hSBEM mRNA is a promising molecular biomarker for detecting breast cancer micrometastases.展开更多
Objective: Using nested reverse transcription-polymerase chain reaction(Nested RT-PCR) to test the mRNA level in peripheral blood CK19 and MUC-1 in colorectal cancer patients and it's clinical significance, to dis...Objective: Using nested reverse transcription-polymerase chain reaction(Nested RT-PCR) to test the mRNA level in peripheral blood CK19 and MUC-1 in colorectal cancer patients and it's clinical significance, to discuss the feasibility of colorectal carcinoma micro-metastasis detection of molecular markers. Methods: The expression level was detected by nested RT-PCR in 20 healthy people, 20 patients with colorectal adenoma and 90 cases of patients with colorectal cancer disease peripheral blood CK19 mRNA and MUC-1 mRNA. Results: The positive expression rate of CK19 mRNA and MUC-1 mRNA were: 58.89%(53/90) and 52.22%(47/90). No CK19 mRNA healthy people 20 cases in the control group in the peripheral blood, the expression of MUC-1 mRNA in 12 cases, the expression rate of 60%(12/20). In 20 cases of colorectal adenoma diseases have the expression of CK19 mRNA in 1 cases, the expression rate of 5%(1/20), the expression of MUC-1 mRNA in 10 cases, the expression rate of 50%. Patients with colorectal cancer CK19 mRNA, MUC-1 mRNA expression rate was significantly correlated with tumor staging, the degree of differentiation of the tumor cells and tumor metastasis(P < 0.05). Conclusion: Marker CK19 mRNA as the detection of micro-metastasis in peripheral blood of patients with colorectal cancer has good sensitivity and specificity, but CK19 mRNA, MUC-1 mRNA can be used to judge the prognosis of patients with colorectal cancer index.展开更多
文摘An understanding of the knowledge creation and diffusion process in the organizational context is extremely relevant. Because from this understanding, organizations can restructure processes, reorient teams and implement methodologies to assist in the construction of an evolutionary process of knowledge creation and diffusion aimed at sustainable growth and innovation. The theory of complex social networks has been applied in several fields to help understand organizational cognitive processes. However, these approaches still insipiently consider the analysis of the nestedness and modularity of the studied networks. In this article, we presented an approach that sought to identify patterns of nestedness and modularity in networks of affiliation of people in projects in the organizational context. The study sought to identify these patterns in affiliation networks in a public organization providing information technology services in the period from 2006 to 2013. The detection of these patterns was performed using the NODF (Nestedness metric based on Overlap and Decreasing Fill) algorithm described by <a href="#ref1">[1]</a>. The nestedness and modularity metrics can influence patterns of knowledge creation and diffusion in formal and informal networks constituted for the execution of projects in organizations. This study showed that the network structures of the organization during the study period presented a high degree of nestedness, and it was possible to identify combined structures of nestedness and modularity.
文摘We compare and contrast asymmetry and nestedness, two concepts used in the characterisation of the specialist-generalist balance in bipartite ecological interaction networks. Our analysis is relevant to mutualistic networks such as those consisting of flowering plants and pollinators, or fruiting plants and frugivores, or antagonistic networks such as those consisting of plants and herbivores, in an ecological community. We shall refer to the two sets of species in the bipartite network as plants and animals, the usual but not the only ecological situation. By asymmetry we mean either connectivity asymmetry or dependence asymmetry, which are essentially equivalent. Asymmetry expresses two attributes: generalists interact preferentially with specialists, and specialists avoid interacting with each other. Nested patterns, in principle, should express these same two features and one more: the presence of a core of interactions among generalists. We compute the full set of perfectly nested patterns that are possible in an L × L matrix with N interactions representing an ecological network of L plants and L animals, and point out that the number of nested arrangements grows exponentially with N. In addition, we analyse asymmetry for the full set of perfectly nested patterns, and identify extremes of asymmetry inside the universe of nested patterns. The minimal asymmetry is marked by a modular core of interactions between species that are neither specialists nor generalists. On the other hand, the case of maximal asymmetry is formed by a set of few generalists and many specialists with equal connectivity. The stereotypic case of nestedness with a core of interactions among generalists has intermediate asymmetry.
文摘城市中的园林绿地呈现斑块状分布,其栖息地特征与岛屿栖息地相似。2008年11月至2009年10月,对上海市闵行区内的7块城市绿地进行调查,记录雀形目鸟类的分布情况,并运用Nestedness temperature calculator软件,检验其群落结构是否符合嵌套结构。运用Arc GIS软件分析该地区的卫星图片,收集7块样地的面积、绿地盖度、水源距离和人为干扰程度等数据,结合实地调查所得到的数据,分析这一嵌套结构的形成原因和影响因素。调查结果显示:上海市闵行区城市绿地中的雀形目鸟类分布是显著的嵌套结构,矩阵系统温度是21.78℃,填充度41.3%。7块样地中出现的物种数从14种到38种不等。其中,白鹡鸰(Motacilla alba)等11种鸟在7块样地中都有分布,黑卷尾(Dicrurusmacrocerus)等9种鸟类只在一个样地中出现过。园林面积、绿地面积和水源情况都对其嵌套结构有显著影响。但与真正岛屿上存在的群落分布嵌套结构不同,人为干扰程度对这一结构也有非常明显的影响。上述结果表明,影响上海闵行区园林鸟类群落嵌套结构的主要原因是栖息地结构和人为干扰程度。因此,建议在规划和建设城市公园和绿地时,应该偏重于面积较大,植被盖度和丰富度高,结构合理的园林,并尽量减少人为干扰对鸟类栖息地的影响。
文摘Objective： Successful treatment of breast cancer greatly depends on the early detection of its metastasis, therefore a sensitive and specific biomarker for detecting dissemination of the cancer cells will help to achieve this goal. This study was to evaluate the prognostic significance of human small breast epithelial mucin （hSBEM） and CD44V6 in breast cancer. Methods： The expressions of hSBEM mRNA and CD44V6 mRNA were detected with nested reverse transcription polymerase chain reaction （nested RT-PCR） in 67 samples of breast cancer and adjacent normal breast tissue, 16 samples of breast benign lesions tissue, and 67 specimens of peripheral blood from patients with breast cancer, 16 specimens of benign breast lesions, 20 specimens of healthy volunteers, and 25 （each five cases） other carcinomas tissue samples, including those of gastric carcinoma, colorectal carcinoma, esophageal carcinoma, lung carcinoma, and ovary carcinoma, were analyzed for hSBEM mRNA expression by nested RT-PCR. Results： hSBEM mRNA expression was observed in 62/67 （92.54%） of breast cancer, 14/16 （87.50%） of breast benign lesions and 59/67 （88.05%） of normal breast tissue, with no significant differences between them （P 〉 0.05）. None of the samples from other cancer tissues were positive. In peripheral blood the expression of hSBEM mRNA was detected in 34/67 （50.75%） from patients with breast cancer, with significant increasing （P 〈 0.05） in the cases of metastatic disease （stage Ⅳ） and those with lymph node metastasis compared with localized disease （stage Ⅰ, Ⅱ and Ⅲ） and without lymph node metastasis, but its expression was not found in peripheral blood of patients with benign breast lesions or healthy volunteers. Although CD44V6 mRNA was significantly higher in breast cancer than in benign breast lesions tissue and normal breast tissue, its expression in peripheral blood show no significant difference （P 〉 0.05） in the patients with breast cancer （82.09%）, benign breast lesion （75.00%）, or healthy volunteers （70.00%）. The expressions of hSBEM mRNA and CD44V6 mRNA had no correlation with the age of the patients, size of primary tumor, histological type and estrogen or progestin receptor status （P 〉 0.05）. Conclusion： hSBEM mRNA, as assessed by nested RT-PCR, shows a mammary-specific and mammary-sensitive expression, and is a sensitive indicator of hematogeneous spread of breast cancer cell, while CD44V6 shows low sensitivity and specificity in detecting dissemination of breast cancer cell in peripheral blood, hSBEM mRNA is a promising molecular biomarker for detecting breast cancer micrometastases.
文摘Objective: Using nested reverse transcription-polymerase chain reaction(Nested RT-PCR) to test the mRNA level in peripheral blood CK19 and MUC-1 in colorectal cancer patients and it's clinical significance, to discuss the feasibility of colorectal carcinoma micro-metastasis detection of molecular markers. Methods: The expression level was detected by nested RT-PCR in 20 healthy people, 20 patients with colorectal adenoma and 90 cases of patients with colorectal cancer disease peripheral blood CK19 mRNA and MUC-1 mRNA. Results: The positive expression rate of CK19 mRNA and MUC-1 mRNA were: 58.89%(53/90) and 52.22%(47/90). No CK19 mRNA healthy people 20 cases in the control group in the peripheral blood, the expression of MUC-1 mRNA in 12 cases, the expression rate of 60%(12/20). In 20 cases of colorectal adenoma diseases have the expression of CK19 mRNA in 1 cases, the expression rate of 5%(1/20), the expression of MUC-1 mRNA in 10 cases, the expression rate of 50%. Patients with colorectal cancer CK19 mRNA, MUC-1 mRNA expression rate was significantly correlated with tumor staging, the degree of differentiation of the tumor cells and tumor metastasis(P < 0.05). Conclusion: Marker CK19 mRNA as the detection of micro-metastasis in peripheral blood of patients with colorectal cancer has good sensitivity and specificity, but CK19 mRNA, MUC-1 mRNA can be used to judge the prognosis of patients with colorectal cancer index.
