[Objectives] This study was conducted to establish the genetic marker Bi-PASA of STCH gene in Jinfen white pig and New Shanxi black pig. [Methods] The polymorphism of STCH gene was detected in Jinfen white pig and New...[Objectives] This study was conducted to establish the genetic marker Bi-PASA of STCH gene in Jinfen white pig and New Shanxi black pig. [Methods] The polymorphism of STCH gene was detected in Jinfen white pig and New Shanxi black pig by bidirectional PCR amplification of specific alleles. [Results] There were two alleles(A, B) and three genotypes(AA, AB and BB) in Jinfen white pig and New Shanxi black pig. The PopGene32 analysis showed that the polymorphic site of STCH gene in Jinfen white pig accorded with the Hardy-Weinberg equilibrium(P>0.05), while that of new Shanxi black pig did not meet the Hardy-Weinberg equilibrium(P<0.01). The results of chi-square test showed that the polymorphic site of STCH gene was significantly different in genotype distribution between the two pig breeds(P<0.01). [Conclusions] The establishment of the genetic marker Bi-PASA of STCH gene in Jinfen white pig and new Shanxi black pig could provide basic biological data for improving disease resistance of pigs and establishing disease-resistant populations.展开更多
Previous studies have documented the occurrence of glyphosate-resistant （GR） goosegrass （Eleusine indica （L.） Gaertn.） and, in at least some cases, resistance is due to an altered target site. Research was perfo...Previous studies have documented the occurrence of glyphosate-resistant （GR） goosegrass （Eleusine indica （L.） Gaertn.） and, in at least some cases, resistance is due to an altered target site. Research was performed to determine if an altered target site was responsible for GR in a Tennessee, United States goosegrass population （TennGR）. DNA sequencing revealed a mutation in TennGR plants conferring the Prol06Ser 5-enolpyruvylshikimate-3-phosphate synthase （EPSPS） substitution previously identified in other GR populations. F2 populations were derived from TennGR plants crossed with plants from a glyphosate-susceptible population （TennGS） and analyzed for their response to glyphosate and genotyped at the EPSPS locus. Plants from the F2 populations segregated 1：2：1 sensitive：intermediate：resistant in response to a selec- tive dose of glyphosate, and these responses co-segregated with the EPSPS genotypes （PP106, PS106, and SS106）. To separately investigate the effect of the Prol06Ser substitution on GR, glyphosate dose-response curves and 50% effective dose （EDso） values were compared among the three genotypes and the two parental populations. The SS106 genotype was 3.4-fold resistant relative to the PP106 genotype, identical to the resistance level obtained when comparing the resistant and susceptible parental populations. We conclude that the mutation conferring a Prol06Ser EPSPS mutation is solely responsible for GR in the TennGR goosegrass population.展开更多
A polyhedral active set algorithm PASA is developed for solving a nonlinear optimization problem whose feasible set is a polyhedron. Phase one of the algorithm is the gradient projection method, while phase two is any...A polyhedral active set algorithm PASA is developed for solving a nonlinear optimization problem whose feasible set is a polyhedron. Phase one of the algorithm is the gradient projection method, while phase two is any algorithm for solving a linearly constrained optimization problem. Rules are provided for branching between the two phases. Global convergence to a stationary point is established, while asymptotically PASA performs only phase two when either a nondegeneracy assumption holds, or the active constraints are linearly independent and a strong second-order sufficient optimality condition holds.展开更多
基金Supported by Doctoral Scientific Research Staring Foundation(YQ-20190010)Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(2019L0878)
文摘[Objectives] This study was conducted to establish the genetic marker Bi-PASA of STCH gene in Jinfen white pig and New Shanxi black pig. [Methods] The polymorphism of STCH gene was detected in Jinfen white pig and New Shanxi black pig by bidirectional PCR amplification of specific alleles. [Results] There were two alleles(A, B) and three genotypes(AA, AB and BB) in Jinfen white pig and New Shanxi black pig. The PopGene32 analysis showed that the polymorphic site of STCH gene in Jinfen white pig accorded with the Hardy-Weinberg equilibrium(P>0.05), while that of new Shanxi black pig did not meet the Hardy-Weinberg equilibrium(P<0.01). The results of chi-square test showed that the polymorphic site of STCH gene was significantly different in genotype distribution between the two pig breeds(P<0.01). [Conclusions] The establishment of the genetic marker Bi-PASA of STCH gene in Jinfen white pig and new Shanxi black pig could provide basic biological data for improving disease resistance of pigs and establishing disease-resistant populations.
文摘Previous studies have documented the occurrence of glyphosate-resistant （GR） goosegrass （Eleusine indica （L.） Gaertn.） and, in at least some cases, resistance is due to an altered target site. Research was performed to determine if an altered target site was responsible for GR in a Tennessee, United States goosegrass population （TennGR）. DNA sequencing revealed a mutation in TennGR plants conferring the Prol06Ser 5-enolpyruvylshikimate-3-phosphate synthase （EPSPS） substitution previously identified in other GR populations. F2 populations were derived from TennGR plants crossed with plants from a glyphosate-susceptible population （TennGS） and analyzed for their response to glyphosate and genotyped at the EPSPS locus. Plants from the F2 populations segregated 1：2：1 sensitive：intermediate：resistant in response to a selec- tive dose of glyphosate, and these responses co-segregated with the EPSPS genotypes （PP106, PS106, and SS106）. To separately investigate the effect of the Prol06Ser substitution on GR, glyphosate dose-response curves and 50% effective dose （EDso） values were compared among the three genotypes and the two parental populations. The SS106 genotype was 3.4-fold resistant relative to the PP106 genotype, identical to the resistance level obtained when comparing the resistant and susceptible parental populations. We conclude that the mutation conferring a Prol06Ser EPSPS mutation is solely responsible for GR in the TennGR goosegrass population.
基金supported by the National Science Foundation of USA(Grant Nos.1522629 and 1522654)the Office of Naval Research of USA(Grant Nos.N00014-11-1-0068 and N00014-15-12048)+1 种基金the Air Force Research Laboratory of USA(Contract No.FA8651-08-D-0108/0054)National Natural Science Foundation of China(Grant No.11571178)
文摘A polyhedral active set algorithm PASA is developed for solving a nonlinear optimization problem whose feasible set is a polyhedron. Phase one of the algorithm is the gradient projection method, while phase two is any algorithm for solving a linearly constrained optimization problem. Rules are provided for branching between the two phases. Global convergence to a stationary point is established, while asymptotically PASA performs only phase two when either a nondegeneracy assumption holds, or the active constraints are linearly independent and a strong second-order sufficient optimality condition holds.
