Over-summering is a crucial period for Calanus sinicus in the southern Yellow Sea,where it is a key member of the zooplankton community.Lipids play an important role in copepod diapause,which is part of their over-sum...Over-summering is a crucial period for Calanus sinicus in the southern Yellow Sea,where it is a key member of the zooplankton community.Lipids play an important role in copepod diapause,which is part of their over-summering strategy.We investigated how different fatty acids and lipid classes,including wax esters,changed during over-summering of C.sinicus during three cruises in June and August 2011 and November 2010,corresponding to the pre-,during and post-diapause periods,respectively.Large amounts of lipids were accumulated,mainly wax esters as previously found in C.finmarchicus during its diapause,and most of the storage lipids were used during over-summering.Wax ester polyunsaturated fatty acids(PUFAs) showed the most variation of the fatty acids(FAs),while the percentage composition of FAs in polar lipids was relatively stable.Selective use of wax ester PUFAs has already been shown to play important roles in the winter diapause of Calanus species in other regions,and our FA results show that this is the case for the Yellow Sea Cold Bottom Water(YSCBW) population that diapauses in summer.展开更多
Nitrogen removal from media by microalgae provides the potential benefit of producing lipids for biodiesel and biomass. However, research is limited on algal growth and biomass under different nitrogen sources and pro...Nitrogen removal from media by microalgae provides the potential benefit of producing lipids for biodiesel and biomass. However, research is limited on algal growth and biomass under different nitrogen sources and provides little insight in terms of biofuel production. We studied the influences of nitrogen sources on cell growth and lipid accumulation of Chlamydomonas sp. ICE-L, one of a promising oil rich micro algal species. Chlamydomonas sp.ICE-L grown in NH_4 Cl medium had maximum growth rate. While the highest dry biomass of 0.28 g/L was obtained in media containing NH_4NO_3, the highest lipid content of 0.21 g/g was achieved under nitrogendeficiency condition with a dry biomass of 0.24 g/L. In terms of total polyunsaturated fatty acids（PUFAs）production, NH_4NO_3 and NH_4 Cl media performed better than nitrogen-deficiency and KNO_3 media.Furthermore, NH_4NO_3 and NH_4 Cl media elucidated better results on C18：3 and C20：5 productions while KNO_3and-N conditions were better in C16：0, C18：1 and C18：2, comparatively.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.41076099,41121064,41406159)the National Basic Research Program of China(973 Program)(No.2011CB403604)
文摘Over-summering is a crucial period for Calanus sinicus in the southern Yellow Sea,where it is a key member of the zooplankton community.Lipids play an important role in copepod diapause,which is part of their over-summering strategy.We investigated how different fatty acids and lipid classes,including wax esters,changed during over-summering of C.sinicus during three cruises in June and August 2011 and November 2010,corresponding to the pre-,during and post-diapause periods,respectively.Large amounts of lipids were accumulated,mainly wax esters as previously found in C.finmarchicus during its diapause,and most of the storage lipids were used during over-summering.Wax ester polyunsaturated fatty acids(PUFAs) showed the most variation of the fatty acids(FAs),while the percentage composition of FAs in polar lipids was relatively stable.Selective use of wax ester PUFAs has already been shown to play important roles in the winter diapause of Calanus species in other regions,and our FA results show that this is the case for the Yellow Sea Cold Bottom Water(YSCBW) population that diapauses in summer.
基金The National Natural Science Foundation of China under contract No.41576187the National Natural Science Foundation of China–Shandong Joint Fund under contract No.U1406402+6 种基金the Basic Scientific Fund for National Public Research Institutes of China under contract No.2015G10the Polar Strategic Foundation of China under contract No.20150303the Public Science and Technology Research Funds Projects of Ocean under contract No.201405015the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology and the Science under contract No.2015ASKJ02the Science and Technology Planning Project of Shandong Province under contract No.2014GHY115003the Major Projects of Independent Innovation Achievements Transformation in Shandong Province under contract No.2014ZZCX06202Qingdao Entrepreneurship and Innovation Pioneers Program under contract No.15-10-3-15-(44)-zch
文摘Nitrogen removal from media by microalgae provides the potential benefit of producing lipids for biodiesel and biomass. However, research is limited on algal growth and biomass under different nitrogen sources and provides little insight in terms of biofuel production. We studied the influences of nitrogen sources on cell growth and lipid accumulation of Chlamydomonas sp. ICE-L, one of a promising oil rich micro algal species. Chlamydomonas sp.ICE-L grown in NH_4 Cl medium had maximum growth rate. While the highest dry biomass of 0.28 g/L was obtained in media containing NH_4NO_3, the highest lipid content of 0.21 g/g was achieved under nitrogendeficiency condition with a dry biomass of 0.24 g/L. In terms of total polyunsaturated fatty acids（PUFAs）production, NH_4NO_3 and NH_4 Cl media performed better than nitrogen-deficiency and KNO_3 media.Furthermore, NH_4NO_3 and NH_4 Cl media elucidated better results on C18：3 and C20：5 productions while KNO_3and-N conditions were better in C16：0, C18：1 and C18：2, comparatively.
