Ultrastructural changes in secondary wall formation of Phyllostachys pubescens Mazel fiber were investigated with transmission electron microscopy. Fiber developed initially with the elongation of cells containing...Ultrastructural changes in secondary wall formation of Phyllostachys pubescens Mazel fiber were investigated with transmission electron microscopy. Fiber developed initially with the elongation of cells containing ribosomes, mitochondria and Golgi bodies in the dense cytoplasm. During the wall thickening, the number of rough endoplasmic reticulum and Golgi bodies increased apparently. There were two kinds of Golgi vesicles, together with the ones from endoplasmic reticulum formed transport vesicles. Many microtubules were arranged parallel to the long axis of the cell adjacent to the plasmalemma. Along with the further development of fiber, polylamellate structure of the secondary wall appeared, with concurrent agglutination of chromatin in the nucleus, swelling and disintegration of organelles, while cortical microtubules were still arranged neatly against the inner side of plasmalemma. Lomasomes could be observed between the wall and plasmalemma. The results indicated that the organelles, such as Golgi bodies together with small vesicles, rough endoplasmic reticulum and lomasomes, played the key role in the thickening and lignification of the secondary wall of bamboo fiber, though cortical microtubules were correlative with the process as well.展开更多
[Objective] This study aimed to explore the heterogeneity of chlorophyll flu- orescence of Phyllostachys edulis cv. Pachyloen leaves. [Method] Using the chloro- phyll fluorescence system and fluorescence imaging syste...[Objective] This study aimed to explore the heterogeneity of chlorophyll flu- orescence of Phyllostachys edulis cv. Pachyloen leaves. [Method] Using the chloro- phyll fluorescence system and fluorescence imaging system in the Mini-IMAGING- PAM, the chlorophyll fluorescence of Phyllostachys edulis cv. Pachyloen leaves were measured and the fluorescence parameters were calculated. [Result] The homo- geneities of the maximal quantum yield of PS II （Fv/Fm） and the leaf absorptivity （Abs） were higher, with CVs （Coefficient of Variation） of 1.58%-1.68% and 1.75%- 2.12% respectively, while the heterogeneities of the actual quantum yield （Y）, non- photochemical quenching （NPQ/4）, photochemical quenching（qP） and relative photo- synthetic rate （PS/50） were higher, with CVs of 9.60%-14.23%, 10.23%-13.02%, 11.92%-13.02% and 11.15%-17.74% respectively. The trends of heterogeneity change in Y, qP and PSI50 were basically the same with transversely higher het- erogeneity at two sides and lower heterogeneity in the middle, namely with larger CVs at the edges of leaves and smaller ones around the midrib. Longitudinally, the coefficients of variation of Y, qP and PS/50 decreased gradually （from top to bot- tom）, which indicated that the heterogeneity declined from the leaf tip to the base. The trends of heterogeneity change in PS/50 and NPQ/4 were opposite. [Conclusion] The fluorescence parameters of Phyllostachys edulis cv. Pachyloen leaves revealed different heterogeneity.展开更多
文摘Ultrastructural changes in secondary wall formation of Phyllostachys pubescens Mazel fiber were investigated with transmission electron microscopy. Fiber developed initially with the elongation of cells containing ribosomes, mitochondria and Golgi bodies in the dense cytoplasm. During the wall thickening, the number of rough endoplasmic reticulum and Golgi bodies increased apparently. There were two kinds of Golgi vesicles, together with the ones from endoplasmic reticulum formed transport vesicles. Many microtubules were arranged parallel to the long axis of the cell adjacent to the plasmalemma. Along with the further development of fiber, polylamellate structure of the secondary wall appeared, with concurrent agglutination of chromatin in the nucleus, swelling and disintegration of organelles, while cortical microtubules were still arranged neatly against the inner side of plasmalemma. Lomasomes could be observed between the wall and plasmalemma. The results indicated that the organelles, such as Golgi bodies together with small vesicles, rough endoplasmic reticulum and lomasomes, played the key role in the thickening and lignification of the secondary wall of bamboo fiber, though cortical microtubules were correlative with the process as well.
基金Supported by the Special Fund for Basic Research of International Center for Bamboo and Rattan(1632011005)~~
文摘[Objective] This study aimed to explore the heterogeneity of chlorophyll flu- orescence of Phyllostachys edulis cv. Pachyloen leaves. [Method] Using the chloro- phyll fluorescence system and fluorescence imaging system in the Mini-IMAGING- PAM, the chlorophyll fluorescence of Phyllostachys edulis cv. Pachyloen leaves were measured and the fluorescence parameters were calculated. [Result] The homo- geneities of the maximal quantum yield of PS II （Fv/Fm） and the leaf absorptivity （Abs） were higher, with CVs （Coefficient of Variation） of 1.58%-1.68% and 1.75%- 2.12% respectively, while the heterogeneities of the actual quantum yield （Y）, non- photochemical quenching （NPQ/4）, photochemical quenching（qP） and relative photo- synthetic rate （PS/50） were higher, with CVs of 9.60%-14.23%, 10.23%-13.02%, 11.92%-13.02% and 11.15%-17.74% respectively. The trends of heterogeneity change in Y, qP and PSI50 were basically the same with transversely higher het- erogeneity at two sides and lower heterogeneity in the middle, namely with larger CVs at the edges of leaves and smaller ones around the midrib. Longitudinally, the coefficients of variation of Y, qP and PS/50 decreased gradually （from top to bot- tom）, which indicated that the heterogeneity declined from the leaf tip to the base. The trends of heterogeneity change in PS/50 and NPQ/4 were opposite. [Conclusion] The fluorescence parameters of Phyllostachys edulis cv. Pachyloen leaves revealed different heterogeneity.
