A bacterium strain B26 capable of producing(R)-α-hydroxyphenylacetic acid [(R)-HPA](yield,47.5%;enantiomeric excess,99.1%) from phenylglyoxylic acid(PGA) with high optical purity was isolated and identified as Bacill...A bacterium strain B26 capable of producing(R)-α-hydroxyphenylacetic acid [(R)-HPA](yield,47.5%;enantiomeric excess,99.1%) from phenylglyoxylic acid(PGA) with high optical purity was isolated and identified as Bacillus sp.B26 by 16S rDNA(ribosomal DNA) sequencing.Phylogenic analysis showed that the strain was most similar to Bacillus sp.enrichment culture clone SYW5(FJ601635.1) and Bacillus cereus strain FM-4(EU794727.1).Efforts were made to further improve HPA-production and PGA-tolerance by UV irradiation and UV-LiCl cooperative mutagenesis.Among viable mutants,B.sp.UV-38 and B.sp.ULi-11 exhibited better productivities than the wild type.Comparisons of HPA production and time course among wild strain and two mutants showed that B.sp.ULi-11 was more competent than B.sp.UV-38.HPA production was increased by 39.1% with B.sp.ULi-11(yield,65.4%) compared to that with B.sp.B26(yield,47.0%) when cultured in fermentation broth(pH 7.2) at 32℃ with an agitation speed of 180 r·min-1 and PGA final concentration of 15 mmol·L-1 for 25 h.展开更多
应变-旋转(Strain-Rotation,S-R)和分解定理为分析几何非线性问题提供了合理可靠的理论基础,但用有限元求解时会遇到大变形发生后的网格畸变问题。近年提出的虚单元法(Virtual element method,VEM)适用于一般的多边形网格,因此,该文尝...应变-旋转(Strain-Rotation,S-R)和分解定理为分析几何非线性问题提供了合理可靠的理论基础,但用有限元求解时会遇到大变形发生后的网格畸变问题。近年提出的虚单元法(Virtual element method,VEM)适用于一般的多边形网格,因此,该文尝试使用一阶虚单元求解基于S-R和分解定理的二维几何非线性问题,以克服网格畸变的影响。基于重新定义的多项式位移空间基函数,推演获得一阶虚单元分析线弹性力学问题时允许位移空间向多项式位移空间的投影表达式;按照虚单元法双线性格式的计算规则,分析处理基于更新拖带坐标法和势能率原理的增量变分方程;进而建立离散系统方程及其矩阵表达形式,并编制MATLAB求解程序;采用常规多边形网格和畸变网格,应用该文算法分析均布荷载下的悬臂梁和均匀内压下的厚壁圆筒变形。结果与已有文献和ANSYS软件的对比表明:该文算法在两种网格中均可有效执行且具备足够数值精度。总体该文算法为基于S-R和分解定理的二维几何非线性问题求解提供了一种鲁棒方法。展开更多
基金Supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China (20103514110002)the Science and Technology Key Project of Fujian Province (2009N0046)
文摘A bacterium strain B26 capable of producing(R)-α-hydroxyphenylacetic acid [(R)-HPA](yield,47.5%;enantiomeric excess,99.1%) from phenylglyoxylic acid(PGA) with high optical purity was isolated and identified as Bacillus sp.B26 by 16S rDNA(ribosomal DNA) sequencing.Phylogenic analysis showed that the strain was most similar to Bacillus sp.enrichment culture clone SYW5(FJ601635.1) and Bacillus cereus strain FM-4(EU794727.1).Efforts were made to further improve HPA-production and PGA-tolerance by UV irradiation and UV-LiCl cooperative mutagenesis.Among viable mutants,B.sp.UV-38 and B.sp.ULi-11 exhibited better productivities than the wild type.Comparisons of HPA production and time course among wild strain and two mutants showed that B.sp.ULi-11 was more competent than B.sp.UV-38.HPA production was increased by 39.1% with B.sp.ULi-11(yield,65.4%) compared to that with B.sp.B26(yield,47.0%) when cultured in fermentation broth(pH 7.2) at 32℃ with an agitation speed of 180 r·min-1 and PGA final concentration of 15 mmol·L-1 for 25 h.
文摘热轧带钢是钢铁行业的重要产品,其表面缺陷是影响产品质量的重要因素。针对传统缺陷检测算法存在的过程繁琐、精度不足和效率低下等问题,提出一种基于改进更快速区域卷积神经网络(faster region-based convolutional neural network,Faster R-CNN)的检测算法,实现对热轧带钢表面缺陷的高效、高精度检测。首先,采用特征相加的方法对底层细节特征和高层语义特征进行融合;然后,采用精准的感兴趣区域池化(precise region of interest pooling,Precise ROI Pooling)获取固定大小的特征向量,避免特征出现位置偏差;最后,利用均值偏移聚类算法对带钢数据集进行聚类,获得适用于热轧带钢表面缺陷检测的先验框尺寸。实验结果表明,所提算法在热轧带钢表面缺陷检测数据集上的平均精度均值达到了85.34%,检测速度为23.5帧/s,且鲁棒性良好,满足实际的工业检测需求。
文摘应变-旋转(Strain-Rotation,S-R)和分解定理为分析几何非线性问题提供了合理可靠的理论基础,但用有限元求解时会遇到大变形发生后的网格畸变问题。近年提出的虚单元法(Virtual element method,VEM)适用于一般的多边形网格,因此,该文尝试使用一阶虚单元求解基于S-R和分解定理的二维几何非线性问题,以克服网格畸变的影响。基于重新定义的多项式位移空间基函数,推演获得一阶虚单元分析线弹性力学问题时允许位移空间向多项式位移空间的投影表达式;按照虚单元法双线性格式的计算规则,分析处理基于更新拖带坐标法和势能率原理的增量变分方程;进而建立离散系统方程及其矩阵表达形式,并编制MATLAB求解程序;采用常规多边形网格和畸变网格,应用该文算法分析均布荷载下的悬臂梁和均匀内压下的厚壁圆筒变形。结果与已有文献和ANSYS软件的对比表明:该文算法在两种网格中均可有效执行且具备足够数值精度。总体该文算法为基于S-R和分解定理的二维几何非线性问题求解提供了一种鲁棒方法。
