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Synergistic effect of carbon nanotube and encapsulated carbon layer enabling high-performance SnS_2-based anode for lithium storage 被引量:1
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作者 Chunwei Dong Yongjin Xia +7 位作者 Zhijiang Su Zhihua Han Yang Dong Jingyun Chen Fei Hao Qiyao Yu Qing Jiang Jiaye Ye 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第10期700-709,I0015,共11页
Tin disulfide(SnS_(2)),due to large interlayer spacing and high theoretical capacity,is regarded as a prospective anode material for lithium-ion batteries.Nevertheless,the poor electron conductivity of SnS_(2) and hug... Tin disulfide(SnS_(2)),due to large interlayer spacing and high theoretical capacity,is regarded as a prospective anode material for lithium-ion batteries.Nevertheless,the poor electron conductivity of SnS_(2) and huge volumetric change during the lithiation/delithiation process lead to a rapid capacity decay of the battery,hindering its commercialization.To address these issues,herein,SnS_(2) is in-situ grown on the surface of carbon nanotubes(CNT)and then encapsulated with a layer of porous amorphous carbon(CNT/SnS_(2)@C)by simple solvothermal and further carbonization treatment.The synergistic effect of CNT and porous carbon layer not only enhances the electrical co nductivity of SnS_(2) but also limits the huge volumetric change to avoid the pulverization and detachment of SnS_(2).Density functional theo ry calculations show that CNT/SnS_(2)@C has high Li^(+)adsorption and lithium storage capacity achieving high reaction kinetics.Consequently,cells with the CNT/SnS_(2)@C anode exhibit a high lithium storage capacity of 837mAh/g after 100 cycles at 0.1 A/g and retaining a capacity of 529.8 mAh/g under 1.0 A/g after 1000 cycles.This study provides a fundamental understanding of the electrochemical processes and beneficial guidance to design high-performance SnS_(2)-based anodes for LIBs. 展开更多
关键词 Lithium-ion batteries Porous amorphous carbon Carbon nanotubes SnS_(2)-based anode Density functional theory calculations
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High-temperature BaTiO_(3) -based ceramic capacitors by entropy engineering design
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作者 Yan Song Min Zhang +4 位作者 Shun Lan Bingbing Yang Yiqian Liu Ce-Wen Nan Yuan-Hua Lin 《Journal of Advanced Ceramics》 SCIE EI CAS CSCD 2024年第9期1498-1504,共7页
High-performance BaTiO_(3)(BTO)-based dielectric ceramics have great potential for high-power energy storagedevices. However, its poor temperature reliability and stability due to its low Curie temperature impedes the... High-performance BaTiO_(3)(BTO)-based dielectric ceramics have great potential for high-power energy storagedevices. However, its poor temperature reliability and stability due to its low Curie temperature impedes the development ofmost electronic applications. Herein, a series of BTO-based ceramics are designed and prepared on the basis of entropyengineering. Owing to the incorporation of Bi(Mg_(0.5)Ti_(0.5))O_(3), relaxation behavior and low dielectric loss at high temperatureshave been achieved. Moreover, the high-entropy strategy also promotes lattice distortion, grain refinement and excellentresistance, which together increase the breakdown field strength. These simultaneous effects result in outstanding energystorage performance, ultimately achieving stable energy density (U_(e)) of 5.76 J·cm^(−3) and efficiency ( η) of 89%. Mostimportantly, the outstanding temperature stability makes high-entropy BTO-based ceramics realize a significant energystorage density of 4.90 0.14 J·cm^(−3) with the efficiency above 89%, spanning a wide temperature range of 25–250 ℃, aswell as cycling reliability with negligible performance deterioration after 3 105 cycles at 300 kV∙cm^(−1) and 200 ℃. Thisresearch presents an effective method for designing temperature-stable and reliable dielectrics with comprehensive energystorage performance. 展开更多
关键词 BaTiO_(3)-based ceramics high-entropy effects high-temperature energy storage cycling reliability
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RNAi在水稻病虫害防控中的应用研究进展
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作者 饶玉春 吴日成 +1 位作者 刘富远 戴若惠 《浙江师范大学学报(自然科学版)》 CAS 2024年第4期361-369,共9页
近年来,来自病原微生物和各类害虫的威胁限制了水稻的生长发育和稳产,亟需一种绿色可控的技术进行有效的生物防治.RNAi技术能够使病虫害相关基因表达下调,具有特异性强、安全性高等优点,相较于化学农药防治,RNAi技术大大降低了非靶标生... 近年来,来自病原微生物和各类害虫的威胁限制了水稻的生长发育和稳产,亟需一种绿色可控的技术进行有效的生物防治.RNAi技术能够使病虫害相关基因表达下调,具有特异性强、安全性高等优点,相较于化学农药防治,RNAi技术大大降低了非靶标生物(non-target organisms, NTOs)的危害.在农业领域,RNAi技术被寄予厚望,被誉为“农药史上的第三次革命”.通过查阅国内外相关文献,并分析、归纳后,综述了近年来RNAi在水稻抗病毒中的作用研究进展,重点阐述了水稻抗病虫害领域应用的最新研究成果,并针对RNAi沉默效率低、稳定性差等问题提出了不同RNAi的增强策略,为实现水稻病虫害精准高效的防治提供现实依据. 展开更多
关键词 rnai 水稻 病虫害防控 作用模型 策略
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基于RNAi的生物农药研究进展
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作者 路子琪 王静 +3 位作者 张震 王教瑜 孙国仓 林福呈 《浙江农业学报》 CSCD 北大核心 2024年第4期968-977,共10页
化学农药的大量使用产生的病虫害抗性、环境污染和农产品安全等一系列问题,使得新型可持续的植物保护策略成为农业绿色发展的必然要求。近年来,RNAi在植物病虫害防控中的应用潜力得到越来越多的关注,基于RNAi的生物农药(即核酸农药)的... 化学农药的大量使用产生的病虫害抗性、环境污染和农产品安全等一系列问题,使得新型可持续的植物保护策略成为农业绿色发展的必然要求。近年来,RNAi在植物病虫害防控中的应用潜力得到越来越多的关注,基于RNAi的生物农药(即核酸农药)的研发越来越迅速。核酸农药以喷雾诱导基因沉默(SIGS)为基础,通过外源施用针对靶标病原菌或害虫的dsRNA,即可触发RNAi从而抑制病原菌或害虫,实现对靶标病虫害特异性控制。由于核酸农药具靶标特异性、环境友好、不易产生抗性等优势,相关研究和开发进程日益加快,使之有望成为化学农药的重要补充或替代。文章对核酸农药的作用机制、研究进展、产品研发和应用现状,在商业化和使用过程中的风险以及需要注意的问题进行了综述,以期为相关研究提供参考。 展开更多
关键词 核酸农药 rnai DSRNA 病虫害防控
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甜菜BvPCNA基因RNAi载体构建
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作者 王希 张西宁 +3 位作者 陶艳昵 张琦 李志烨 赵春雷 《中国糖料》 2024年第2期9-16,共8页
【目的】增殖细胞核抗原(Proliferating cell nuclear antigen, PCNA)在植物中广泛存在,但是其在植物中的功能研究较少。本研究可以为BvPCNA-like基因的功能分析奠定基础。【方法】课题组前期在甜菜基因组中克隆获得一个基因位点,命名为... 【目的】增殖细胞核抗原(Proliferating cell nuclear antigen, PCNA)在植物中广泛存在,但是其在植物中的功能研究较少。本研究可以为BvPCNA-like基因的功能分析奠定基础。【方法】课题组前期在甜菜基因组中克隆获得一个基因位点,命名为BvPCNA-like基因,本研究以BvPCNA-like基因的克隆载体为初始材料,选取适宜的靶序列,利用植物表达载体pFGC5941作为基础载体,使靶序列上游启动子为35 S,植物筛选标记为抗除草剂基因BlpR,设计靶序列特异性引物,采取PCR-酶切-连接方法,构建BvPCNA-RNAi载体。【结果】通过RCR鉴定表明已获得了预期目标载体,插入片段由正向靶序列BvPCNA-F、中间片段、反向靶序列BvPCNA-R组成,靶序列长度为320 bp。【结论】本研究所得载体适用于对双子叶植物进行遗传转化,可以作为BvPCNA-like基因理论研究或应用研究的载体材料。 展开更多
关键词 甜菜 BvPCNA-like rnai 载体构建
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First-principles study of moderate phonon-mediated pairing in high-pressure monoclinic phase of BiS_(2)-based superconductors
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作者 程杰 程玉兰 +1 位作者 李斌 刘胜利 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第10期625-629,共5页
Isotope effect on superconductive transition temperature(T_c)is an essential indicator to examine whether the mechanism of superconductors is conventional.Unconventional isotope effect of BiS_(2)-based superconductors... Isotope effect on superconductive transition temperature(T_c)is an essential indicator to examine whether the mechanism of superconductors is conventional.Unconventional isotope effect of BiS_(2)-based superconductors has been previously reported in ambient-pressure tetragonal phase.However,to comprehensively ascertain the nature of superconductivity,the investigation of BiS_(2)-based system in high-pressure structure is highly desirable.In this work,we carried out the first-principles calculations of phonon spectra and superconductivity in high-pressure monoclinic phase of LaO_(0.5)F_(0.5)BiS_(2)with ^(32)S and ^(34)S,and observed that the corresponding isotope coefficient is 0.13≤α≤0.20.This value is much greater than that of BiS_(2)-based superconductors in ambient-pressure phase,but slightly smaller than that of conventional MgB_2.Taking into account the calculated T_(c) lower than experimental results,we finally conclude that the moderate phonon-mediated pairing plays a significant role in forming superconductivity of BiS_(2)-based system in high-pressure phase,moreover,the cooperative multiple paring interactions should also be considered. 展开更多
关键词 BiS_(2)-based superconductor high pressure isotope effect phonon-mediated pairing
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Rational design of MoS_(2)-based catalysts toward lignin hydrodeoxygenation:Interplay of structure,catalysis,and stability
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作者 Xinyong Diao Na Ji 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2023年第2期601-631,I0015,共32页
The MoS_(2)-based materials are a vital class of heterogeneous catalysts for the hydrodeoxygenation of lignin and its model compounds to produce value-added chemicals especially because of their unique selectivity to ... The MoS_(2)-based materials are a vital class of heterogeneous catalysts for the hydrodeoxygenation of lignin and its model compounds to produce value-added chemicals especially because of their unique selectivity to aromatics.The rational design of MoS_(2)-based catalyst greatly depends on the comprehensive understanding of its structure-activity relationship.However,an intensive summary and critical analysis are still scarce to date.In this review,we attempt to provide an in-depth understanding of the interplay of structure,catalysis,and stability of MoS_(2)-based catalysts for lignin hydrodeoxygenation.The recognition of intrinsic active sites on MoS_(2) structure was firstly discussed,followed by the illustration of MoS_(2)-catalyzed hydrodeoxygenation structural models.Afterward,based on the studies on the MoS_(2)-catalyzed lignin model compounds hydrodeoxygenation,the current active site modification strategies including structural modification of monometallic MoS_(2) catalysts and collaborative modification were summarized and emphatically discussed,which aims to elucidate the structure-activity relationship at the atomic-level.The deactivation mechanism and stabilization strategies were also illustrated to provide instructive suggestion for the rational design of efficient and stable MoS_(2)-based catalysts.Finally,the real lignin depolymerization over MoS_(2)-based catalysts was summarized to point out the advantages and difficulties.This review attempts to highlight the remaining challenges and provide some perspectives for the future development of MoS_(2)-based catalysts for lignin hydrodeoxygenation. 展开更多
关键词 Lignin hydrodeoxygenation MoS_(2)-based catalysts Structure-activity relationship Modification strategies Deactivation mechanism
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Ferroelectricity of hafnium oxide-based materials:Current status and future prospects from physical mechanisms to device applications
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作者 Wanwang Yang Chenxi Yu +9 位作者 Haolin Li Mengqi Fan Xujin Song Haili Ma Zheng Zhou Pengying Chang Peng Huang Fei Liu Xiaoyan Liu Jinfeng Kang 《Journal of Semiconductors》 EI CAS CSCD 2023年第5期78-121,共44页
The finding of the robust ferroelectricity in HfO_(2)-based thin films is fantastic from the view point of both the fundamentals and the applications.In this review article,the current research status of the future pr... The finding of the robust ferroelectricity in HfO_(2)-based thin films is fantastic from the view point of both the fundamentals and the applications.In this review article,the current research status of the future prospects for the ferroelectric HfO_(2)-based thin films and devices are presented from fundamentals to applications.The related issues are discussed,which include:1)The ferroelectric characteristics observed in HfO_(2)-based films and devices associated with the factors of dopant,strain,interface,thickness,defect,fabrication condition,and more;2)physical understanding on the observed ferroelectric behaviors by the density functional theory(DFT)-based theory calculations;3)the characterizations of microscopic and macroscopic features by transmission electron microscopes-based and electrical properties-based techniques;4)modeling and simulations,5)the performance optimizations,and 6)the applications of some ferroelectric-based devices such as ferroelectric random access memory,ferroelectric-based field effect transistors,and the ferroelectric tunnel junction for the novel information processing systems. 展开更多
关键词 FERROELECTRICITY HfO_(2)-based thin films physical mechanism characterization modeling and simulation applications
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RNAi技术在烟粉虱基因功能和防控研究中的应用
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作者 韦焕文 苏润馨 +1 位作者 吴屿瞳 史晓斌 《生物灾害科学》 2024年第3期321-330,共10页
烟粉虱(Bemisia tabaci)是一种世界性蔬菜害虫,可传播多种植物病毒,影响植物生长发育,导致蔬菜产量下降。烟粉虱的防治以化学防治为主,但过度使用杀虫剂导致烟粉虱的抗药性不断增强,绿色防控的需求日益增加。RNA干扰(RNA interference,R... 烟粉虱(Bemisia tabaci)是一种世界性蔬菜害虫,可传播多种植物病毒,影响植物生长发育,导致蔬菜产量下降。烟粉虱的防治以化学防治为主,但过度使用杀虫剂导致烟粉虱的抗药性不断增强,绿色防控的需求日益增加。RNA干扰(RNA interference,RNAi)是一项特异性沉默靶标基因技术,广泛应用于昆虫的基因功能研究和绿色防控。通过介绍RNAi作用原理和RNAi在烟粉虱的基因功能,总结了RNAi在烟粉虱的防控应用和RNA农药的应用现状,为烟粉虱的新靶点研究和开发RNA农药进行烟粉虱防控应用提供参考。 展开更多
关键词 rnai 烟粉虱 基因功能 RNA农药 生物防治
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A spawning particle filter for defocused moving target detection in GNSS-based passive radar
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作者 ZENG Hongcheng DENG Jiadong +3 位作者 WANG Pengbo ZHOU Xinkai YANG Wei CHEN Jie 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2023年第5期1085-1100,共16页
Global Navigation Satellite System(GNSS)-based passive radar(GBPR)has been widely used in remote sensing applications.However,for moving target detection(MTD),the quadratic phase error(QPE)introduced by the non-cooper... Global Navigation Satellite System(GNSS)-based passive radar(GBPR)has been widely used in remote sensing applications.However,for moving target detection(MTD),the quadratic phase error(QPE)introduced by the non-cooperative target motion is usually difficult to be compensated,as the low power level of the GBPR echo signal renders the estimation of the Doppler rate less effective.Consequently,the moving target in GBPR image is usually defocused,which aggravates the difficulty of target detection even further.In this paper,a spawning particle filter(SPF)is proposed for defocused MTD.Firstly,the measurement model and the likelihood ratio function(LRF)of the defocused point-like target image are deduced.Then,a spawning particle set is generated for subsequent target detection,with reference to traditional particles in particle filter(PF)as their parent.After that,based on the PF estimator,the SPF algorithm and its sequential Monte Carlo(SMC)implementation are proposed with a novel amplitude estimation method to decrease the target state dimension.Finally,the effectiveness of the proposed SPF is demonstrated by numerical simulations and pre-liminary experimental results,showing that the target range and Doppler can be estimated accurately. 展开更多
关键词 Global Navigation Satellite System(GNSS)-based passive radar(GBPR) defocused target moving target detec-tion(MTD) likelihood ratio function(LRF) spawning particle fil-ter(SPF)
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Effect of VC/Cr_3C_2 on microstructure and mechanical properties of Ti(C,N)-based cermets 被引量:6
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作者 詹斌 刘宁 +2 位作者 金之铂 李其龙 石锦罡 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2012年第5期1096-1105,共10页
Effects of VC/Cr3C2 on the microstructure and mechanical properties of Ti(C,N)-based cermets were studied. The microstructure was investigated by means of optical microscopy, X-ray diffractometry as well as scanning... Effects of VC/Cr3C2 on the microstructure and mechanical properties of Ti(C,N)-based cermets were studied. The microstructure was investigated by means of optical microscopy, X-ray diffractometry as well as scanning electron microscopy in combination with energy dispersive spectrometry. Mechanical properties, such as transverse rupture strength, hardness and fracture toughness, were measured. The results show that there are black core-grey rim structure and white core-grey rim structure in the microstructure. The grains become fine due to the VC/Cr3C2, and the grains of cermet added with 0.75VC/0.25Cr3C2 are refined most remarkably. The black core becomes finer with the increase of VC addition and rim phase becomes thicker with the decrease of Cr3C2 addition. The porosity increases with the increase of VC addition in VC/Cr3C2. Compared with the cermet free of VC/Cr3C2, the transverse rupture strength and hardness of cermets with VC/Cr3C2 are both improved, and the maximum values are both found for the cermet with 0.25VC/0.75Cr3C2. The fracture toughness can be effectively promoted by adding VC/Cr3C2 with an appropriate ratio of VC to Cr3C2, and the maximum value is found for the cermet with 0.5VC/0.5Cr3C2. 展开更多
关键词 Ti(C N)-based cermet VC CR3C2 microstructure mechanical properties
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Research progress of Ag_3PO_4-based photocatalyst: Fundamentals and performance enhancement 被引量:3
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作者 马运柱 成帆 +2 位作者 刘文胜 王娟 王依锴 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2015年第1期112-121,共10页
Ag3PO4 is found to be a highly efficient photocatalyst and receives great attention. The high activity of the photocatalyst is credited to the intrinsic electronic structure. The morphology control and nano-composite ... Ag3PO4 is found to be a highly efficient photocatalyst and receives great attention. The high activity of the photocatalyst is credited to the intrinsic electronic structure. The morphology control and nano-composite fabrication are used to improve the performance and practicability. This paper reviews the structure, properties and some theoretical aspects of Ag3PO4 single crystal. Also, the major strategies, namely the morphology control and hetero-nanostructure construction, as ways to improve the performance of Ag3PO4-based photocatalysts, are summarized with the aid of some typical instances. 展开更多
关键词 Ag3PO4-based photocatalyst electronic structure morphology control hetero-structure construction preparation photocatalytic activity
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Design of high strength Fe-(P,C)-based bulk metallic glasses with Nb addition 被引量:1
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作者 郭胜锋 沈烨 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2011年第11期2433-2437,共5页
Bulk metallic glass(BMG) rods Fe71Mo5-xNbxP12C10B2(x=1,2,3,4 and 5) with diameter of 1 or 2 mm were synthesized by copper mold casting.The effects of Nb substitution for Mo on the structure,thermal and mechanical ... Bulk metallic glass(BMG) rods Fe71Mo5-xNbxP12C10B2(x=1,2,3,4 and 5) with diameter of 1 or 2 mm were synthesized by copper mold casting.The effects of Nb substitution for Mo on the structure,thermal and mechanical properties of Fe71Mo5-xNbxP12C10B2 alloys were studied by X-ray diffraction,differential scanning calorimetry and compressive testing.The results show that the substitution of Nb for Mo leads to a decreased glass forming ability,but with plasticity of 1.0%,the fracture strength of Fe71Mo2Nb3P12C10B2 alloy increases up to 4.0 GPa.The improvement of the fracture strength is discussed in terms of the enhancement of atomic bonding nature and the favorite formation of a network-like structure due to the substitution of Nb for Mo. 展开更多
关键词 Fe-(P C)-based bulk metallic glass NB forming ability mechanical properties
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SnO_2-based gas(hydrogen) anodes for aluminum electrolysis 被引量:3
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作者 肖赛君 Tommy MOKKELBOST +2 位作者 Ove PAULSEN Arne P.RATVIK Geir M.HAARBERG 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2014年第12期3917-3921,共5页
A novel SnO2-based gas anode was developed for aluminum electrolysis in molten cryolite at 850 &#176;C to reduce energy consumption and decrease CO2 emissions. Hydrogen was introduced into the anode, participating in... A novel SnO2-based gas anode was developed for aluminum electrolysis in molten cryolite at 850 &#176;C to reduce energy consumption and decrease CO2 emissions. Hydrogen was introduced into the anode, participating in the anode reaction. Carbon and aluminum were used as the cathode and reference electrodes, respectively. Cyclic voltammetry was applied in the cell to investigate the electrochemical behavior of oxygen ion on platinum and SnO2-based materials. The potential for oxygen evolution on these electrode materials was determined. Then, galvanostatic electrolysis was performed on the gas anode, showing a significant depolarization effect (a decrease of ~0.8 V of the anode potential) after the introduction of hydrogen, compared with no gas introduction or the introduction of argon. The results indicate the involvement of hydrogen in the anode reaction (three-phase-boundary reaction including gas, electrolyte and electrode) and give the possibility for the utilization of reducing gas anodes for aluminum electrolysis. 展开更多
关键词 SnO2-based gas anode hydrogen anode aluminum electrolysis
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LINK-A RNAi慢病毒载体构建及其抑制U251胶质瘤细胞增殖的研究 被引量:1
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作者 王军成 兰彦平 +3 位作者 赵岳阳 蒯涛 马东明 李敏 《宁夏医学杂志》 CAS 2023年第7期577-580,F0002,共5页
目的构建lncRNA LINK-A的特异性RNAi慢病毒载体,并借此敲减人U251胶质瘤细胞株内的LINK-A,探究LINK-A对U251胶质瘤细胞增殖的影响。方法针对人LINK-A设计siRNA序列,构建RNAi慢病毒载体质粒并进行测序鉴定;利用pHelper 1.0、pHelper 2.0... 目的构建lncRNA LINK-A的特异性RNAi慢病毒载体,并借此敲减人U251胶质瘤细胞株内的LINK-A,探究LINK-A对U251胶质瘤细胞增殖的影响。方法针对人LINK-A设计siRNA序列,构建RNAi慢病毒载体质粒并进行测序鉴定;利用pHelper 1.0、pHelper 2.0以及shRNAi 3种质粒分别转染293T细胞,筛选分离慢病毒并测定滴度;用PBS及构建好的慢病毒感染人U251胶质瘤细胞,实验分3组:空白组(PBS)、NC组(空载病毒)、LINK-A(-)组(LINK-A RNAi慢病毒),72 h后利用RT-PCR检测细胞中LINK-A的表达情况;取病毒感染成功后的U251胶质瘤细胞行平板克隆实验培养10 d,行CCK-8实验分别于24 h、48 h检测。结果RNAi慢病毒载体质粒测序结果与RNAi设计序列一致,提示LINK-A RNAi慢病毒载体构建成功,滴度为3×108 TU/mL;病毒感染U251胶质瘤细胞72h后,细胞生长状态良好,荧光持续稳定表达,LINK-A RNA相对表达量明显降低(P<0.05),表明病毒成功感染U251细胞;10 d后LINK-A(-)组细胞克隆形成数及形成率较空白组及NC组明显减少(P<0.05);与空白组及NC组相比,24 h后LINK-A(-)组OD值减少(P<0.05),72 h明显减少(P<0.05)。结论人LINK-A RNAi慢病毒构建成功;LINK-A表达降低可抑制U251胶质瘤细胞增殖。 展开更多
关键词 胶质瘤 增殖 LINK-A rnai
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Split-GFP双分子荧光互补技术在鸡MSTN基因RNAi检测中的应用
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作者 杨磊 朱正 +4 位作者 王博永 梁谦学 吴文德 李恭贺 郑喜邦 《南方农业学报》 CAS CSCD 北大核心 2023年第8期2444-2453,共10页
【目的】以Split-GFP双荧光互补技术检测鸡肌肉生长抑制素基因(MSTN)RNA干涉(RNAi)效果,并与其他常用检测方法比较,以验证Split-GFP双分子荧光互补技术在RNAi效果评估中的有效性和可行性。【方法】将合成的3个shRNA慢病毒载体(shRNA-a、... 【目的】以Split-GFP双荧光互补技术检测鸡肌肉生长抑制素基因(MSTN)RNA干涉(RNAi)效果,并与其他常用检测方法比较,以验证Split-GFP双分子荧光互补技术在RNAi效果评估中的有效性和可行性。【方法】将合成的3个shRNA慢病毒载体(shRNA-a、shRNA-b和shRNA-c)分别转染稳定表达GFP11-MSTN融合蛋白的HEK 293TGFP11-MSTN细胞,经实时荧光定量PCR筛选出最佳shRNA慢病毒载体并包装为慢病毒,然后以慢病毒感染HEK 293TGFP11-MSTN细胞,采用潮霉素B筛选mCherry阳性(mCherry+)细胞,再以实时荧光定量PCR和Western blotting检测RNAi效果;得到的mCherry+细胞再转染pcDNA3.1(+)-GFP1-10质粒,通过荧光显微镜观察和流式细胞术评估RNAi效果。【结果】3个shRNA慢病毒载体对MSTN基因表达均有极显著的抑制作用(P<0.01,下同),其中又以Anti-MSTN shRNA-a慢病毒载体的干涉效果最佳。Anti-MSTN shRNA-a慢病毒感染HEK 293TGFP11-MSTN细胞的最适MOI=3,该条件下MSTN基因相对表达量及GFP11-MSTN融合蛋白表达量均受到抑制;得到的mCherry+细胞再转染pcDNA3.1(+)-GFP1-10质粒,荧光显微镜观察和流式细胞术检测结果表明,GFP+细胞数量明显减少,GFP+细胞百分率由31.1%降至11.5%。Split-GFP检测结果与实时荧光定量PCR及Western blotting检测结果相符,说明Anti-MSTN shRNA-a慢病毒能有效抑制GFP11-MSTN融合蛋白表达,发挥了RNAi作用。【结论】以Anti-MSTN shRNA-a慢病毒对MSTN基因的干涉效果最佳,其感染HEK 293TGFP11-MSTN细胞后MSTN基因表达极显著下调,且再转染pcDNA3.1(+)-GFP1-10质粒后细胞中的GFP+细胞百分率明显下降,与实时荧光定量PCR和Western blotting检测结果相符,证实Slipt-GFP双分子荧光互补技术是一种可靠的可视化RNAi检测方法。 展开更多
关键词 肌肉生长抑制素(MSTN) RNA干涉(rnai) shRNA 慢病毒 Split-GFP双分子荧光互补技术
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对PKM2进行RNAi可提高环磷酰胺对PC3细胞的抑制作用
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作者 王潇然 于欣 +7 位作者 王永杰 王勇 廉吉虎 李震霄 董春丽 宋海涛 张靓靓 张海涛 《中国老年学杂志》 CAS 北大核心 2023年第20期5072-5075,共4页
目的目的:探讨对丙酮酸激酶同工酶(PK)M2进行RNAi提高环磷酰胺(CTX)对PC3细胞的抑制作用。方法通过联合CTX和表达PKM2的短发夹RNA(shRNA)质粒用于人PC3细胞株,观察其对PC3细胞生长的影响。结果pshRNA-Con组细胞增殖率明显高于其他3组,ps... 目的目的:探讨对丙酮酸激酶同工酶(PK)M2进行RNAi提高环磷酰胺(CTX)对PC3细胞的抑制作用。方法通过联合CTX和表达PKM2的短发夹RNA(shRNA)质粒用于人PC3细胞株,观察其对PC3细胞生长的影响。结果pshRNA-Con组细胞增殖率明显高于其他3组,pshRNA-PKM2+CTX组细胞增殖率明显低于pshRNA-PKM2组和CTX组(均P<0.05)。pshRNA-PKM2组和CTX组细胞增殖率差异无统计学意义(P>0.05)。pshRNA-PKM2+CTX组细胞凋亡率明显高于其他3组(均P<0.05)。第18天后,pshRNA-Con组同一时间点肿瘤体积均明显高于其他3组,pshRNA-PKM2+CTX组同一时间点肿瘤体积均明显低于其他3组(均P<0.05)。结论PKM2是调节肿瘤细胞有氧糖酵解的关键酶,抑制PKM2可抑制肿瘤生长。 展开更多
关键词 丙酮酸激酶M2 环磷酰胺 人前列腺癌细胞株 rnai
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家蚕RNAi效率相关核酸酶基因对RNAi效率的影响 被引量:1
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作者 陈咏琪 尹延萍 +4 位作者 冯嘉伟 白新宇 李庆荣 钟仰进 杨婉莹 《昆虫学报》 CAS CSCD 北大核心 2023年第3期303-311,共9页
【目的】鳞翅目(Lepidoptera)昆虫消化系统存在的核酸酶是导致RNAi低效的主要原因之一,本研究旨在探索家蚕Bombyx mori RNAi效率相关核酸酶(RNAi efficiency-related nuclease, REase)BmREase对家蚕RNAi低效的影响。【方法】利用RT-PCR... 【目的】鳞翅目(Lepidoptera)昆虫消化系统存在的核酸酶是导致RNAi低效的主要原因之一,本研究旨在探索家蚕Bombyx mori RNAi效率相关核酸酶(RNAi efficiency-related nuclease, REase)BmREase对家蚕RNAi低效的影响。【方法】利用RT-PCR对家蚕BmREase cDNA全长序列进行同源克隆和生物信息学分析,并采用最大似然法进行系统发育分析;利用qRT-PCR检测BmREase在游走期家蚕不同组织(头、表皮、脂肪体、中肠、气管、马氏管和丝腺)中的特异性表达。通过向游走期家蚕注射BmREase,家蚕蜕皮激素受体(ecdysone receptor, EcR)基因BmEcR,超气门蛋白(ultraspiracle, USP)基因BmUSP和细胞因子sp?tzle1(BmSpz1)基因BmSpz1的dsRNA进行RNAi,分析干扰BmREase表达后是否可以提高靶基因dsRNAs的干扰效率。【结果】RT-PCR扩增得到家蚕BmREase(GenBank登录号:XM_021350017.2)全长cDNA序列,其ORF长2 241 bp,编码746个氨基酸残基;生物信息学分析发现,BmREase与人核酸外切酶I 3qe9.1具有极其相似的结构,其Thr7, His33, Ala37, Arg93, Lys97, Tyr159, Asp160, Ser161和Asn174组成的活性结构域能够与核苷酸序列结合,暗示BmREase具有核酸酶活性。qRT-PCR结果显示,BmREase在家蚕游走期的中肠和马氏管中高表达,说明BmREase主要在家蚕消化系统中表达。在家蚕游走期注射dsRNA,BmREase的表达量比空白对照组的高,RNAi干扰BmREase提高了dsBmEcR, dsBmUSP和dsBmSpz1的干扰效率。【结论】家蚕体内存在与人类核酸外切酶相似功能的酶BmREase,其存在可能影响dsRNA在家蚕体内的干扰效果。本研究为利用RNAi进行家蚕基因功能的研究以及进一步开发RNAi防治害虫具有指导性意义。 展开更多
关键词 家蚕 RNA干扰 DSRNA rnai效率相关核酸酶 BmREase dsRNA外切酶
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;~lasmid-based Survivin shRNA and GRIM-19 carried by attenuated Salmonella suppresses tumor cell growth 被引量:7
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作者 Yan-Bo Liu Ling Zhang +7 位作者 Ya-Xiong Guo Li-Fang Gao Xi-Chun Liu Li-Juan Zhao Bao-Feng Guo Li-Jing Zhao Xue-Jian Zhao De-Qi Xu 《Asian Journal of Andrology》 SCIE CAS CSCD 2012年第4期536-545,共10页
Persistent activation of Survivin and its overexpression contribute to the formation, progression and metastasis of several different tumor types. Therefore, Survivin is an ideal target for RNA interference mediated-g... Persistent activation of Survivin and its overexpression contribute to the formation, progression and metastasis of several different tumor types. Therefore, Survivin is an ideal target for RNA interference mediated-growth inhibition. Blockade of Survivin using specific short hairpin RNAs (shRNA) can significantly reduce prostate tumor growth. RNA interference does not fully ablate target gene expression, owing to the idiosyncrasies associated with shRNAs and their targets. To enhance the therapeutic efficacy of Survivin-specific shRNA, we employed a combinatorial expression of Survivin-specific shRNA and gene associated with retinoid-interferon-induced mortality-19 (GRIM-19). Then, the GRIM-19 coding sequences and Survivin-specific shRNAs were used to create a dual expression plasmid vector and were carried by an attenuated strain of Salmonella enteric serovar typhimurium (S. typhimurium) to treat prostate cancer in vitro and in vivo. We found that the co-expressed Survivin-specific shRNA and GRIM-19 synergistically and more effectively inhibited prostate tumor proliferation and survival, when compared with treatment with either single agent alone in vitroand in vivo. This study has provided a novel cancer gene therapeutic approach for prostate cancer. 展开更多
关键词 GRIM-19 prostate cancer rnai Salmonella enterica serovar typhimurium SURVIVIN tumor cell growth
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Lentinan prolonged survival in patients with gastric cancer receiving S-1-based chemotherapy 被引量:10
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作者 Kenji Ina Ryuichi Furuta +5 位作者 Takae Kataoka Satoshi Kayukawa Takashi Yoshida Takaya Miwa Yoshitaka Yamamura Yuuki Takeuchi 《World Journal of Clinical Oncology》 CAS 2011年第10期339-343,共5页
AIM:To examine whether administration of lentinan,purifiedβ-1,3-glucan,can prolong survival in advanced gastric cancer patients receiving S-1-based chemotherapy.METHODS:Since 2004,78 patients with metastatic or recur... AIM:To examine whether administration of lentinan,purifiedβ-1,3-glucan,can prolong survival in advanced gastric cancer patients receiving S-1-based chemotherapy.METHODS:Since 2004,78 patients with metastatic or recurrent gastric cancer have received S-1-based chemotherapy as first-line treatment.Survival,side effects,and the ratio of granulocytes/lymphocytes(G/L ratio)were compared between 2 groups of patients who received chemo-immunotherapy using lentinan and chemotherapy alone.RESULTS:Median overall survival was significantly longer in the former group than in the latter group[689 d(95%CI:431-2339 d)vs 565 d(95%CI:323-662 d),P=0.0406].In addition,the G/L ratio in patients who received lentinan was maintained around or below 2,which was significantly lower than that in patients who received chemotherapy alone(P<0.001).CONCLUSION:Chemo-immunotherapy with lentinan offers a significant advantage over S-1-based chemotherapy alone in terms of survival in patients with advanced gastric cancer. 展开更多
关键词 Gastric cancer LENTINAN S-1-based CHEMOTHERAPY
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